Recent Articles

Below are the titles and abstracts from the most recent articles published in In Vitro Cellular and Developmental Biology – Plant. Click on the title to view the full article at Springerlink.


  • Correction to: An efficient micropropagation protocol for Monochasma savatieri Franch. ex Maxim through seed germination and direct shoot regeneration
    on January 20, 2021 at 12:00 am

    A Correction to this paper has been published:

  • In vitro morpho-physiological performance and DNA stability of banana under cadmium and drought stresses
    on January 15, 2021 at 12:00 am

    Abstract The combined influence of cadmium (Cd) and drought was evaluated on in vitro regenerated plants of banana ‘Grand Nain’ at morpho-physiological and molecular levels. A significant reduction was observed due to separate or combined treatments of Cd and polyethylene glycol (PEG) in total chlorophyll, carotenoid content, and all growth parameters except root length, which was increased under Cd treatment. Meanwhile, other traits were increased significantly under combined treatment of Cd and PEG (1 and 3%), including malondialdehyde, phenolic content, and peroxidase specific activity. Furthermore, molecular analysis using the inter-simple sequence repeats (ISSR) markers confirmed the harmful effect of Cd. Cadmium caused DNA damage reflected by 18.4% polymorphism compared to the control. Out of 14 polymorphic bands, 12 were absent due to Cd treatment and two bands existed only in Cd-treated samples compared with control. However, the addition of PEG was […]

  • In vitro regeneration, antioxidant potential, and genetic fidelity analysis of Asystasia gangetica ( L. ) T.Anderson
    on January 14, 2021 at 12:00 am

    Abstract An effective protocol for the plant regeneration via direct and indirect organogenesis has been developed from leaf explants of Asystasia gangetica (L.), cultured on Murashige and Skoog (MS) medium supplemented with various concentrations and combinations of auxin and cytokinins. Approximately 86% of explants produced direct shoots on MS medium containing 0.5 mg L−1 6-benzyladenine (BA) and 10 μg L−1 Triacontanol (TRIA) with a maximum of 4.82 ± 0.29 shoots per leaf segment. For production of callus-mediated plantlets (indirect), primarily callus was induced on MS medium containing 2 mg L−1 2,4-dichlorophenoxyacetic acid (2,4-D), which was then subcultured on medium with 0.1 mg L−1 naphthaleneacetic acid (NAA), 0.5 mg L−1 BA, and 1 to 8 mg L−1 2-isopentenyl adenine (2iP) in order to develop organogenic callus and subsequent shoot induction. A maximum of 6.84 ± 0.05 shoots per callus clump was obtained on MS media […]

  • Induction and early identification of tetraploid black locust by hypocotyl in vitro
    on January 14, 2021 at 12:00 am

    Abstract Tetraploid black locust (Robinia pseudoacacia L.) has advantages over diploid varieties in growth and stress resistance. Previous studies had shown that tetraploid black locust can be induced only by treating shoot tips of the germinating seeds with colchicine. In order to establish a simpler and more efficient tetraploid induction method in black locust, we induced tetraploid plants by treating the hypocotyls from in vitro–grown seedlings with different concentrations of colchicine, and then provided a method for tetraploid identification based on stomatal size and density in this study. The highest induction efficiency was 53.33% by treating hypocotyl explants with 70 mg·L−1 colchicine for 2 d. Tetraploid black locust was significantly different from diploid in morphological characteristics. Leaf area, leaf thickness, chlorophyll content, stomatal length, and stomatal width were greater in tetraploid plants than in diploid plants, whereas the […]

  • Effect of coconut water and cytokinins on rapid micropropagation of Ranunculus wallichianus Wight & Arnn—a rare and endemic medicinal plant of the Western Ghats, India
    on January 8, 2021 at 12:00 am

    Abstract Ranunculus wallichianus is a rare medicinal plant endemic to Western Ghats, India. Nodal explants were inoculated on MS medium with 1.0 to 5.0 mg L−1 of 6-benzyladenine (BA), kinetin (KIN), or thidiazuron (TDZ) resulting in a low percentage of shoot regeneration. Coconut water (CW) was added to MS medium containing the above mentioned cytokinins to promote in vitro plantlet growth. Multiple shoots were regenerated on half-strength Murashige and Skoog (MS) medium containing 1.0 to 5.0 mg L−1 BA, KIN, or TDZ in combination with 5%, 10%, and 20% CW. The highest percentage of shoot multiplication was observed from cultures incubated on half-strength MS medium supplemented with 3.0 mg L−1 TDZ in combination with 10% CW. The cut ends of well-elongated shoots were transferred to medium containing 0.5 to 3.0 mg L−1 indole-3-acetic acid (IAA) or indole-3-butyric acid (IBA) to induce in vitro root induction. Half-strength MS medium with 2.5 mg L−1 […]

  • Immunolocalization of some arabinogalactan protein epitopes during indirect somatic embryogenesis and shoot organogenesis in leaf culture of centaury ( Centaurium erythraea Rafn)
    on January 8, 2021 at 12:00 am

    Abstract Common centaury (Centaurium erythraea Rafn) is a medicinal plant of great importance for both pharmaceutical and food industries. Distribution of arabinogalactan proteins (AGPs) with specific epitopes recognized by seven monoclonal antibodies (mAbs) was investigated during indirect somatic embryogenesis (ISE) and shoot organogenesis (ISO) in centaury leaf culture. Dynamic changes were observed in localization of JIM4, JIM8, JIM13, JIM15, LM2, LM14, and MAC207 epitopes during somatic embryo (SE) and adventitious bud (AB) development. AGPs responded to all tested mAbs and expressed in numerous meristematic centers that were formed on leaf explants. In globular SEs, the distribution pattern of JIM4, JIM13, JIM15, LM2, and MAC207 epitopes was observed, while during the progression of somatic embryo development, the number of detected AGPs decreased. During formation of ABs, the number of detected AGPs also decreased. In fully formed ABs, only JIM4 and MAC207 […]

  • Comparative transcriptome analysis reveals molecular regulators underlying pluripotent cell induction and callus formation in Anthurium andraeanum “Alabama”
    on January 8, 2021 at 12:00 am

    Abstract Shoot regeneration from pluripotent cell masses is an important process for genetic improvement of Anthurium andraeanum through Agrobacterium-mediated transformation. However, the mechanisms underlying pluripotent cell induction are not well understood. Here, three half-leaf explant samples collected after 0-, 2-, and 30-d culture on callus-inducing medium were used for RNA isolation and transcriptome profiling. In total, 225,752 non-redundant unigenes were assembled with a mean length of 1299 bp. Differentially expressed genes among the three samples were identified by comparative transcriptome analysis. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis showed the greatest number of differentially expressed genes in the comparison between 0- and 2-d cultures were related to the “ribosome.” Many differentially expressed genes in the comparison between 2- and 30-d cultures were involved in “plant hormone signal transduction,” “starch and […]

  • The effect of alternative membrane system, sucrose, and culture methods under photosynthetic photon flux on growth and volatile compounds of mint in vitro
    on January 8, 2021 at 12:00 am

    Abstract The aims of the present study were to evaluate the effects of different ventilation systems and sucrose concentrations on the growth, photosynthetic pigment content, and chemical composition of Mentha arvensis L. and M. viridis L. Photon flux (PPF) and growth were also evaluated based on M. arvensis heterotrophic, photomixotrophic, and photoautotrophic culture systems. Nodal segment explants were cultured on medium with 0, 15, and 30 g L−1 sucrose and four alternative membrane systems (AMS) in culture medium, namely no membrane (NMS), one (AMS1), two (AMS2), and four (AMS4) membranes. Mentha arvensis cultivation on medium containing 15 g L−1 of sucrose and four membrane (AMS4) led to the highest dry weight accumulation. Mentha viridis reached the same result with medium containing 30 g L−1 sucrose and AMS2 and AMS4. Mentha arvensis produced higher chlorophyll a, b and total chlorophyll, as well as carotenoids when cultured on medium with […]

  • In vitro co-culture system for Prunus spp. and Armillaria mellea in phenolic foam rooting matric
    on January 6, 2021 at 12:00 am

    Abstract Armillaria and Desarmillaria spp. are causal agents of a devastating root-borne disease of peach. Breeding resistant rootstock requires a reliable screening tool. An in vitro co-culture screen designed for almond was modified by replacing agar-gelled medium with a more aerated phenolic foam and combining resistant and susceptible rootstocks (i.e., common garden experiment) and minimizes variation in inoculum pressure or rooting substrate among replicate vessels. Eight Prunus rootstocks tested (peach, plum, peach × plum, and choke cherry) were rooted and had no decline in health. Susceptible peach rootstock, ‘GF 305’, was cultured for 15 wk in phenolic foam in the same vessel with a resistant peach × plum hybrid, ‘MP-29’, inoculated with Armillaria mellea at week 5, that led to more severe shoot symptoms in the former after an additional 8 wk. This method accommodated peach genotypes that were difficult to root in agar medium. The difference […]

  • Enhancement of galanthamine production through elicitation and NMR-based metabolite profiling in Narcissus pseudonarcissus cv. Carlton in vitro callus cultures
    on January 5, 2021 at 12:00 am

    Abstract Plants are the unique sources of secondary metabolites (terpenes, phenolic and alkaloid) used as pharmaceuticals, food additives and industrial produces. However, very limited information is known about their biosynthesis in plants. Elicitors are chemicals often used to activate the accumulation of such metabolites in plant in vitro cultures. Since the ancient past, Narcissus is well-known for its ornamental purposes and medicinal versatility with diverse pharmaceutically important alkaloids including galanthamine, lycorine, haemanthamine and narciclasine. In this research, callus cultures induced from N. pseudonarcissus cv. Carlton bulb explants were subjected to MS media supplemented with growth regulators, yeast extract, methyl jasmonate, chitosan and trans-cinnamic acid for the determination of galanthamine content using GC-MS. Bulbs showed 538 to 1109 μg g−1 FW (fresh weight), and callus cultures subjected to MS medium without elicitors produced […]

  • Responses of in vitro cell cultures to elicitation: regulatory role of jasmonic acid and methyl jasmonate: a review
    on January 5, 2021 at 12:00 am

    Abstract Plants are regarded as resources to obtain various industrially important compounds such as pharmaceutical, agrochemical and aromatic products which are produced in their roots, stem, leaves and other aerial parts and mostly extracted from field-grown plants. However, the over-exploitation of plants for secondary metabolites often leads to becoming endangered and in threat of extinction. Owing to these constraints, in vitro culture techniques provide an alternative to overcome problems associated with field production and promotes their conservation. The use of elicitation for the enhancement of secondary metabolite production is gaining importance. Elicitation is defined as an increased production of biomass and secondary metabolites in both in vitro and ex vivo grown plants, subjected to the exposure of different elicitors. The process of elicitation is influenced by culture conditions like elicitor concentration, duration, age and composition of culture. […]

  • Soybean androgenesis II: non-gametophytic morphologies in isolated microspore culture
    on January 5, 2021 at 12:00 am

    Abstract Gametophytic embryogenesis is a process capable of accelerating trait discovery and cultivar development in plant-breeding programs. However, recalcitrance to morphogenic stimuli has limited such work in many crops of interest, especially among fabaceous species such as soybean (Glycine max [L.] Merrill). In complementary studies, donor plant and culture conditions were documented to successfully divert isolated soybean microspores from a gametophytic pathway; these findings were reinforced through transcriptomic analysis of microspores subjected to pretreatment temperature stress, suggesting cellular dedifferentiation through suppression of the pollen developmental program. In the present study, cytological characteristics were observed in isolated microspore culture, which evidenced non-gametophytic development. These phenotypes included (i) inhibition of daughter cell specialization, (ii) pollen dimorphism, and (iii) exine rupture, among others. The […]

  • Shoot organogenesis from leaf discs of the African ginger ( Mondia whitei (Hook.f.) Skeels), an endangered medicinal plant
    on January 5, 2021 at 12:00 am

    Abstract The African ginger (Mondia whitei) is an endangered, endemic African medicinal plant, widely used as an aphrodisiac and to manage gastrointestinal problems and anorexia. Due to high demand, it is over-utilized from the wild without replenishment and destroyed by inappropriate harvesting methods. Therefore, there is a need to develop an efficient conservation system for the African ginger. This study focused on developing an in vitro propagation procedure for the African ginger through indirect shoot organogenesis. Optimal callus formation was induced from leaf explants cultured on Murashige and Skoog (MS) basal medium fortified with 1.5 mg L−1 2,4-dichlorophenoxyacetic acid (2,4-D). The 1.5 mg L−1 6-benzylaminopurine (BAP) concentration was optimal with 50% of the callus-forming buds. For shoot formation, media with 2.0 mg L−1 BAP and 1.0 mg L−1 NAA resulted in 60% of the buds developing shoots. Regenerated shoots were rooted on woody plant […]

  • Cardenolide and glucosinolate accumulation in shoot cultures of Erysimum crepidifolium Rchb .
    on December 4, 2020 at 12:00 am

    Abstract Erysimum crepidifolium Rchb. is one of the few Brassicaceae species accumulating glucosinolates as well as cardenolides. This is possibly providing a selective advantage in evolution as both compounds are part of a chemical defense system. In order to study the biosynthesis of these compounds, a regeneration protocol for E. crepidifolium using in vitro shoot cultures derived from seeds has been developed. Murashige and Skoog (MS) culture medium supplemented with various combinations of cytokinins and auxins was used. MS medium containing NAA (naphthaleneacetic acid, 0.04 mg mL−1) and BAP (6-benzylaminopurine, 0.2·10−2 mg mL−1) proved to be optimal for root formation. Plantlets developed well on modified MS medium without the use of phytohormones. About 80% of the plantlets rooted in vitro developed into intact plants after transfer to the greenhouse. Cardenolides (1.75 mg g−1 dry weight (DW)) were detected in cultured shoots on solid DDV media […]

  • Comparison of shoot tip culture and cryotherapy for eradication of ampeloviruses associated with Pineapple mealybug wilt in wild varieties
    on December 1, 2020 at 12:00 am

    Abstract The Pineapple mealybug wilt-associated virus (PMWaV) provokes a disease that causes considerable losses to pineapple growers due to the reduced leaf tissue turgescence, resulting in leaf necrosis and death in severe cases. In this work, we describe the comparison of shoot tip culture and cryotherapy methods to eradicate the pineapple wilt disease-associated ampeloviruses. Plants from the accessions Ananas comosus var. comosus (AGB-009), var. bracteatus (AGB-119), and var. parguazensis (AGB-376) were indexed by RT-PCR, confirming mixed infections of PMWaV-1, PMWaV-2, and PMWaV-3. The accessions were cultured in vitro and their shoot tips treated by cryotherapy following a droplet vitrification protocol. The regeneration rate from shoot tip culture was 93% for AGB-376 and 100% for the other two accessions. After freezing, AGB-376 had 100% regeneration subsequent to exposure to PVS2 for 45 min, followed by 95% for AGB-009, while for AGB-119 the optimal […]

  • Efficient Chromosome Doubling of an Interspecific Hybrid Dendrobium Stardust ‘Fire Bird’ by Treatment of Amiprofos-methyl to Protocorm-Like Body
    on December 1, 2020 at 12:00 am

    Abstract Dendrobium Stardust ‘Fire Bird’ is an interspecific hybrid between D. unicum and D. Ukon ‘Arai’ and is known to be sterile and prevents further breeding. Chromosome doubling is an essential method to increase the size of plant organs, such as flowers, and restore fertility of interspecific hybrids, which enables further breeding for ornamental traits. To induce chromosome doubling, the protocorm-like bodies (PLBs) of a given variety were treated in vitro for 6 to 48 h with 10 to 160 mg L−1 amiprofos-methyl (APM). Higher APM concentrations resulted in lower rates of survival of treated PLBs. Furthermore, subsequent growth of PLBs was hampered with prolonged APM treatment, although 10 and 20 mg L−1 APM was optimal for doubling chromosomes. Plantlets with a swollen and shorter stem were observed after the APM treatment and these plants were also chromosome-doubled and became a distinctive morphological marker for the primary selection of […]

  • Physical and biochemical differences in Agrobacterium rhizogenes -mediated transgenic hairy root lines of Echinacea purpurea
    on December 1, 2020 at 12:00 am

    Abstract Echinacea (Echinacea purpurea L.), also known as purple coneflower, is one of the important medicinal plants commonly used for respiratory infections. The aim of this study was to compare different transgenic hairy root lines for growth and secondary metabolite biosynthesis and to select a superior hairy root line for the production of caffeic acid derivatives (CADs). Agrobacterium rhizogenes-mediated root lines showing hairy root phenotypic characteristics were analyzed by the Southern blot analysis and the copy number of the rolC gene determined. Significant differences were found among seven hairy root lines evaluated for the copy number of rolC gene, root growth, total phenolic, and CAD contents. It was concluded that these differences may be due to the position and copy number of rolC gene in the genome. Across the parameters compared, it was found that line 7 was superior to the other transgenic lines. The results show that the selection of superior […]

  • Effect of Cytokinins on In Vitro Propagation of Ajuga xylorrhiza Kit Tan (Critically Endangered), Endemic to Turkey
    on December 1, 2020 at 12:00 am

    Abstract Ajuga xylorrhiza Kit Tan is a rare endemic perennial species growing in the stony habitats in Southeastern Anatolia region, Turkey. An efficient and reproducible protocol has been developed for micropropagation of endangered Ajuga xylorrhiza. Shoots were cultured on Murashige and Skoog (MS) basal medium, supplemented with various concentrations of 6-benzyl amino purine (BAP), thidiazuron (TDZ), and kinetin (Kin). The optimal shoot formation was with the medium containing 0.125 mg L−1 BAP (7.91 shoots per explant), and 0.25 mg L−1 Kin (4.9 shoots per explant) was recorded. The best result in terms of shoot length (3.43 to 3.63 cm) was obtained from the Kin treatments. Kin parameters were more effective on shoot length and multiple shoot production than BAP and TDZ. For rooting, regenerated shoots were transferred to 1/4 strength MS medium containing two different concentrations of indole acetic acid (IAA) and α-naphthalenetic acid (NAA) at 0 […]

  • Correction to: Influence of explant types, non-embryogenic synseed and reduced oxygen environment on in vitro conservation of Bacopa monnieri (L.) Wettst
    on December 1, 2020 at 12:00 am

    Readers should note the following error in the original version of this article:

  • A Dedication to Trevor Alleyn Thorpe
    on December 1, 2020 at 12:00 am

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