Recent Articles

Below are the titles and abstracts from the most recent articles published in In Vitro Cellular and Developmental Biology – Plant. Click on the title to view the full article at Springerlink.


  • Induction of somatic embryogenesis and ectopic proliferation in Tecoma stans (L.) Juss. ex Kunth cell suspension culture
    on April 17, 2024 at 12:00 am

    Abstract Somatic embryogenesis is a developmental pathway where somatic cells of plants generate embryogenic cells that subsequently mature into somatic embryos under favorable conditions. This process is one of the most important in vitro techniques for plant propagation, with diverse practical implications. In this study, ectopic proliferation and somatic embryos from Tecoma stans (L.) Juss. ex Kunth cell cultures were induced by employing primary conditioning Murashige and Skoog medium supplemented with 1.0 mg L−1 2,4-dichlorophenoxyacetic acid. Subsequently, a secondary induction medium supplemented with a combination of 1.0 mg L−1 2,4-dichlorophenoxyacetic acid with various concentrations of 6-benzyladenine cytokinin (1 to 5 mg L−1) was used to promote embryogenesis. The results revealed the successful formation of pre-embryonic and embryonic stages, including globular, heart, torpedo, and cotyledon stages within a 2-wk incubation period under the […]

  • Alternative system for micropropagation of Prunus campanulata (Maxim.)
    on April 9, 2024 at 12:00 am

    Abstract Herein, an in vitro multiplication protocol for Prunus campanulata was evaluated. The effects of 15.0, 30.0, and 45.0 g L−1 sucrose and three flask sealing methods (PVC film, rigid polypropylene lid, and rigid lid with membrane) were analyzed at the multiplication, rooting, and acclimatization stages. For in vitro multiplication, shoot tips of approximately 1 cm in length containing two pairs of leaves from seedlings germinated in vitro in Woody Plant Medium (WPM) were used. Evaluations considered the multiplication of shoot apexes in three subcultures (at 40, 80, and 120 d) for survival, number of shoots, shoot length, number of leaves, and multiplication rate. In the rooting phase, the rooting percentage, number of roots, and root length were assessed. In the acclimatization phase under shaded and full sun conditions, seedling survival and biometric characteristics were evaluated, including stem diameter, height, and number of leaves. The addition of […]

  • Correction to: Soybean androgenesis II: non-gametophytic morphologies in isolated microspore culture
    on April 5, 2024 at 12:00 am
  • Growth of in vitro–regenerated plants of Gerbera jamesonii following micropropagation in temporary immersion bioreactors
    on April 1, 2024 at 12:00 am

    Abstract Gerbera represents one of the top five most important traditional cut flowers. A major factor influencing the success of the cut flower industry is vase life which in turn has a significant influence on consumer preference. Therefore, the present study considered the effect of silver nanoparticles (AgNPs) supplied in temporary immersion bioreactors (TIBs) on the growth and subsequent shelf life of gerbera flowers. The results showed an initial lag in the growth of plantlets produced from tissue culture, but this effect was reversed by the end of the study, particularly for plants exposed to AgNPs. Although AgNPs did not shorten the time to flowering (67 d in control plants compared with 73 d in AgNPs-treated plants), flower characteristics were not adversely affected by this delay. A significant finding from this study was the observation of improved vase life of flowers grown from plants treated with AgNPs (9.8 d) relative to control plants (8.2 d). This […]

  • Standardization of in vitro multiplication technique for Areca concinna Thwaites, an endangered palm species for its conservation and utilization
    on March 27, 2024 at 12:00 am

    Abstract Areca concinna Thwaites (Arecaceae) is an endangered plant species endemic to South-Western Sri Lanka and distributed primarily in Sri Lanka, India, and Southeast Asia. This palm was listed under endangered plants species by IUCN (The International Union for Conservation of Nature) due to habitat loss. Clonal propagation is essential for conservation and maintenance of this endangered species. In an effort to conserve this plant species, a protocol was developed for clonal multiplication of A. concinna Thwaites. Immature inflorescence, immature embryos, and mature embryos were tested for callogenesis and subsequent somatic embryogenesis in M72 basal medium supplemented with the auxins 2,4-D and picloram. Of the three explants, somatic embryos were obtained only from mature embryos. Callus multiplication and somatic embryo formation in M72 basal medium supplemented with 2,4-D were found to be better over picloram media. Serial transfer of explants from higher […]

  • Low-temperature storage in dark condition improved the in vitro regeneration of Plumbago zeylanica synthetic seeds: a medicinally valuable species
    on March 11, 2024 at 12:00 am

    Abstract Medicinal applications of Plumbago zeylanica and its metabolites on various diseases and low viability and inconsistent germination of its seeds are the reasons behind the loss of its genetic diversity. Hence, an efficient protocol for the short-term storage of P. zeylanica synthetic seeds, which is an overexploited medicinally valuable plant, was developed. Initially, in vitro culture was performed from nodal explants to develop synthetic seeds from its proliferated shoots. Murashige and Skoog (MS) medium augmented with 0.5 mg L−1 6-benzylaminopurine (BAP) resulted in the best morphogenetic response. Thereafter, the developed synseeds were stored for 2 wk at a temperature of 10 or 25°C in different conditions and further evaluated for regeneration. Higher re-growth rate (80%) and the identical morphogenetic response were recorded for the P. zeylanica synthetic seeds, which were stored at a temperature of 10°C in dark condition after its storage period. […]

  • Effects of different healing agents on acclimatization success of in vitro rooted Garnem (Prunus dulcis × Prunus persica) rootstock
    on March 11, 2024 at 12:00 am

    Abstract Continuing productivity of the acclimatization stage of plantlets means successful micropropagation. Due to the free water and high humidity in the culture container environment, poorly developed cuticle results in rapid water loss and drying of plantlets with watery stems and leaves, incomplete stomata, and large intercellular space. When plantlets are exposed to an environmental condition different from the culture medium, problems, such as rapid water loss and drying, may be encountered, and the survival rate of plantlets in vitro may be reduced. The aim of this study was to reduce the stress factors that occur during the acclimatization phase. For this reason, various healing agents have been used. Some of these compounds are ascorbic acid (AsA), salicylic acid (SA), and sodium nitroprusside (SNP). In the study, the response of AsA (100.0 and 200.0 mg L−1), SA (100.0 and 200.0 mg L−1), and SNP (100.0 and 200.0 µM) applications on growth […]

  • Silver nitrate mediated improvement in micropropagation and amelioration of micro-morpho-anatomical structures in Oxystelma esculentum (L.f.) Sm.
    on March 5, 2024 at 12:00 am

    Abstract The present study highlights the effect of silver nitrate (AgNO3) in the promotion of in vitro regeneration and morpho-structural growth and developments in Oxystelma esculentum (L.f.) Sm. The incorporation of AgNO3 into the Murashige and Skoog (MS) medium resulted in a favorable enhancement of shoot proliferation. Among the different concentrations of AgNO3 screened, 0.5 mg L−1 AgNO3 along with 0.5 mg L−1 meta-Topolin (mT) and 0.25 mg L−1 indole-3-acetic acid (IAA) stimulated the highest count, and length of shoots (143 shoots and 14.0 cm length) after 3 mo of incubation and the 3rd subculture. The in vitro–induced deformities in the tissues of the control stem and foliages were improved when the shoots were grown in AgNO3-enriched medium. Silver nitrate increased the photosynthetic pigments and improved the formation of functional stomata. The AgNO3-supplemented medium-derived shoots exhibited accelerated adventitious root induction (100% […]

  • Establishment of tissue culture regeneration system of Ficus tikoua
    on March 5, 2024 at 12:00 am

    Abstract Ficus tikoua Bureau is an ecologically functional perennial vine that belongs to the Moraceae family. Tissue culture is a common method for the rapid propagation of plants and is suitable for the rapid propagation of F. tikoua. The objective of the current study was to evaluate the process of F. tikoua organogenesis in vitro utilizing stem explants. An efficient tissue culture propagation method was developed by manipulating several plant growth regulators (PGRs) and their proportions, as well as evaluating alternative medium options for specific phases of differentiation. Explants were cultured on MS medium, supplemented with various thidiazuron (TDZ) concentrations with 6-benzylaminopurine (BA) and naphthaleneacetic acid (NAA), to investigate their efficacy during in vitro shoot organogenesis. The results demonstrated that the combination of 0.5 mg L−1 BA, 1.0 mg L−1 NAA, and 1.0 mg L−1 TDZ was the most effective for callus formation (91.1%) and […]

  • Validation of endogenous U6 promoters for expanding the CRISPR toolbox in Nicotiana tabacum
    on March 5, 2024 at 12:00 am
  • Establishment of first protocol of hypocotyl-based regeneration and callus transformation in waterhemp (Amaranthus tuberculatus)
    on February 28, 2024 at 12:00 am

    Abstract Waterhemp (Amaranthus tuberculatus (Moq.) Sauer) is one of the most problematic weeds in the Midwestern agricultural systems in the USA. As a consequence of extensive herbicide selection pressure, many waterhemp populations across the USA evolved resistance to multiple herbicides, leading to limited options for chemical control. Genome editing tools, including CRISPR/Cas9, have great potential in weed science, especially when coupled with a gene drive system for a sustainable weed management. The application of modern biotechnological tools requires availability of an efficient regeneration and genetic transformation system. The objectives of this research were to develop an efficient protocol for successful regeneration of waterhemp via callus culture and optimize an Agrobacterium-mediated transformation method for waterhemp. Hypocotyl explants cultured on callus induction medium (CIM), C6 containing 1.0 mg L−1 kinetin and 4.0 mg L−1 2,4-D for 2 wk […]

  • In vivo regeneration efficiency of enset (Ensete ventricosum (Welw.) Cheesman) using indigenous shoot induction method developed in Ethiopia
    on February 27, 2024 at 12:00 am

    Abstract Enset (Ensete ventricosum [Welw.] Cheesman) is native to Ethiopia, valued for its versatility and drought tolerance. Farmers have developed a vegetative method for regenerating multiple shoots that produce plantlets derived from wound callus, a process similar to shoot production in in vitro tissue culture. This in vivo regeneration method, however, is a low-cost, tissue culture-free technique that is executed in a field context. The objectives of this study were to investigate the efficiency of in vivo regeneration in different enset landraces using the indigenous method of shoot induction and to compare the capability of wild and domesticated enset landraces to regenerate shoots. Three corm treatments (whole, halved, and quartered) from wild and from three domesticated landraces (Gudiro, Bosso, and Yekko), each of which corm or corm pieces with the apical meristem was removed, were buried in a randomized complete block design with three replicates. The […]

  • Direct organ regeneration from apical shoot buds of adult Pinus massoniana Lamb
    on February 21, 2024 at 12:00 am

    Abstract   Direct organogenesis is an important technique for plant rapid propagation, which is mainly controlled by the balance of auxin and cytokinin. Pinus massoniana (Lamb.) is a perennial tree species with high application value. Previous studies have shown that direct organ regeneration of young P. massoniana is feasible. However, there are few reports on direct organogenesis of adult P. massoniana. This research studied the effects of apical shoot bud disinfection, plant growth regulators of axillary bud induction, and adventitious root induction of adult P. massoniana. The results showed that the survival rate could reach 50% after removing the outer coat scales and soaking in 500.0 mg L−1 carbendazim solution for 10 min, 75% alcohol for 30 s, and 2.0% NaClO with one drop of Tween for 25 min. The addition of 0.2% plant preservative mixture (PPM) to the medium effectively inhibited contamination of endogenous bacteria, and the survival rate was 90%. […]

  • In vitro micropropagation of a novel non-flowering radiation mutant of Platanus × acerifolia Willd.
    on February 20, 2024 at 12:00 am

    Abstract An efficient in vitro micropropagation system for Platanus × acerifolia ‘F03’, a radiation mutant that has not flowered for 19 yr, has been established. Axillary buds were used as explants. After sprouting on Murashige and Skoog medium (MS) supplemented with 0.3 mg·L−1 6-benzylaminopurine (6-BA) together with 0.05 mg·L−1 α-naphthaleneacetic acid (NAA), the best medium for multiplication and elongation was MS basal medium supplemented with 0.1 mg·L−1 6-benzylaminopurine and with a multiplication rate of 3.43 ± 0.57. Plantlets over 1.5 cm could root on McCown Woody Plant Medium (WPM) supplemented with 0.01 mg·L−1 α-naphthaleneacetic acid and 7.0 g·L−1 agar with an ideal rooting rate and transplanting survival rate of 100 ± 0%. The optimum survival rate was possible when trays were covered with a thin plastic lid for more than 14 d. After transplanting, air humidity and temperature were crucial for young plant […]

  • Status and prospects of yam somatic embryogenesis: a pathway for biotechnology applications
    on February 20, 2024 at 12:00 am

    Abstract Yam is an important staple in sub-Saharan Africa, but the availability of quality seed yam is majorly constrained by the low propagation ratio. This is because the propagating explant is limited to the tuber and nodal parts as yam rarely flowers. There are several reports of the use of somatic embryogenesis (SE) in the rapid propagation of different crop species and as a regenerative pathway in plant genetic engineering. However, SE deployment in yam is still at the protocol development stage. This review thus exploits the status of SE application in improving the yam propagation rate. This article reviews the potential of the various yam propagation techniques in rapidly multiplying disease-free yam with their propagating explants. The advantages SE offers are rapidly propagating yam, the factors to consider in the protocol optimization of SE application in rapidly multiplying different yam varieties, and as a platform for full utilization of genetic […]

  • Induction, growth, and characteristics of embryonic cell suspension culture of wild bananas (Musa acuminata ssp.)
    on February 14, 2024 at 12:00 am

    Abstract Wild bananas and their relatives are potentially utilized for pre-breeding due to their genetic diversity, disease resistance, and tolerance to abiotic stress, and other desirable traits. The embryonic suspension culture of wild bananas provides a means to harness this genetic diversity for banana genetic improvement. This paper elucidates the response of different subspecies M. acuminata (ssp. malaccensis, microcarpa, sumatrana, and breviformis) to the induction, growth, and behavior of suspension cultures and their regeneration into plantlets. Different subspecies exhibit varied responses starting from the embryogenic culture induction stage, culture proliferation, to plantlet formation. The highest competence for plant regeneration through somatic embryogenesis was found in ssp. malaccensis, followed by microcarpa, sumatrana, and breviformis. The wild banana embryogenic culture consists of somatic embryos, somatic embryo masses, proembryos, and […]

  • Photoautotrophic potential and photosynthetic competence in Ananas comosus [L]. Merr. cultivar Turiaçu in in vitro culture systems
    on February 1, 2024 at 12:00 am

    Abstract The Turiaçu pineapple cultivar produces fruits of high organoleptic value but has few biotechnological studies on seedling production. However, conventional in vitro propagation can affect the photosynthetic potential of plants when transferred to the field, thus mitigating measures should be undertaken to solve this limitation, for example by decreasing carbohydrate concentration in the growth medium, adopting bioreactors of temporary immersion with forced ventilation, and using gas permeable membranes in the culture flask. The present work focused on evaluating the growth and development of plantlets from Ananas comosus [L]. Merr. cultivar Turiaçu, an important but neglected pineapple cultivar, under different sucrose concentrations and cultivation systems. For that, the impact of the photomixotrophic and photoautotrophic growth on morphophysiological responses of plants and survival during the ex vitro acclimatization was assessed. The plants were […]

  • Synseed germination, conversion, and acclimatization of seedless watermelon ‘Quality’
    on February 1, 2024 at 12:00 am

    Abstract Seedless watermelons are triploid hybrids (3n) that have been growing in popularity since the 1990s. However, the high production cost and low germination rate of the hybrid seeds pose a major problem in their production. The synseed technology could make a promising alternative for seedless watermelon propagation; however, this alternative has not yet been explored. This study aimed at producing synseeds of seedless watermelon ‘Quality’ using shoot tips as the propagules after which their germination and conversion were evaluated under in vitro and ex vitro conditions. In vitro shoot multiplication with different concentrations of 6-benzylaminopurine (BAP), synseed production from BAP-multiplied shoot tips, synseed sowing in vitro using several substrates and medium supplements, and synseed sowing ex vitro using different antimicrobial agents were investigated. Results indicated that 1.0 mg L−1 BAP resulted in the highest number of shoots per shoot […]

  • Somatic embryogenesis from the thin cell layer cultures of zygotic and somatic embryos of Aesculus hippocastanum
    on February 1, 2024 at 12:00 am

    Abstract The thin cell layers (TCLs) of zygotic embryos of Aesculus hippocastanum were cultured on Murashige and Skoog (MS) medium with and without 2,4-D. Somatic embryos developed directly on the TCLs of zygotic embryos at the highest frequency on hormone-free medium. The frequency of direct somatic embryo formation was suppressed by 2,4-D treatment. Microscopic observation revealed that compact and nodular structures formed on epidermal surfaces of TCLs, and these structures further developed into somatic embryos without the intervention of the callus stage, indicating direct somatic embryogenesis. Somatic embryos were not formed on the internal excised portion of zygotic embryos. Similar to zygotic embryo culture, direct somatic embryogenesis occurred from the culture of TCLs excised from cotyledonary somatic embryos on hormone-free MS medium. A similar suppression of direct somatic embryo development was observed in the presence of 2,4-D. Instead, friable […]

  • In vitro double haploid production through anther culture in niger (Guizotia abyssinica L.F. Cass)
    on February 1, 2024 at 12:00 am

    Abstract   To keep pace with the skyrocketing demand of vegetable oil and reduced dependency on import, there is a need to focus on indigenous oilseed crops, such as niger (Guizotia abyssinica L.F. Cass). Niger is a minor oilseed crop that consists of 32 to 40% quality oil and 18 to 24% protein in the seeds. Despite its considerable importance, self-incompatibility and high outcrossing pose difficulty in breeding through conventional methods. Double haploid (DH) technology is one of the alternatives to overcome the problem and develop inbred lines in a shorter time. In context to this, a highly efficient protocol for DH production through anther culture in Indian niger varieties JNS 9 and JNS 28 was developed. The investigation was conducted to identify the suitable microspore stage for anther culture, cold pre-treatment, hormonal combinations for callus induction (T1–T4), shooting (S1–S26), rooting (R1–R3), hardening, ploidy index, and the effect of […]

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