For your viewing convenience, links to the 2022 In Vitro Biology Meeting Final Program, Posters and Abstracts are provided below.
SATURDAY, JUNE 4
| 8:30 pm – 10:00 pm |
DESIGN OF EXPERIMENTS WORKSHOP* |
Pacific E |
| PLANT WORKSHOP | Conveners: Uyen Cao Chu, Corteva Agriscience, Randall P. Niedz, USDA, and Todd J. Jones, Corteva Agriscience |
Determining optimal formulations and conditions for improved in vitro growth is often done by testing one ingredient or set of conditions at a time. Design of Experiment (DoE) methodology is a particularly useful set of techniques that allows testing of multiple compounds, processes, or environmental conditions simultaneously in multiplex systems. The principles and statistics behind DoE are complex and require DOE software to design and analyze the experiments. In this workshop, the audience will get an introduction to the use of DOE principles and tools by participating in the design and data analysis of prepared examples relevant to various in vitro culture scenarios.
| 8:30 | Introduction (U. C. Chu and T. J. Jones) | |
| 8:35 | P-30 | DoE Methodology vs. “Standard Stats” – Software for DoE Methodology – DOE Principles and Types of Experiments Randall P. Niedz, USDA |
| 9:00 | PS-31 | DOE Experiment Walkthrough Uyen Cao Chu, Corteva Agriscience |
| 9:25 | Discussion |
SUNDAY, JUNE 5
| 7:30am – 5:30 pm | Registration | Pacific Foyer |
| 7:00 am – 8:00 am | In Vitro Animal Cell Sciences Program Committee Meeting | Pacific H&I |
| 8:00 am – 10:00 am |
ADVANCED APPLICATIONS OF SITE DIRECTED NUCLEASES* |
Pacific C&D |
| PLENARY SYMPOSIUM |
Conveners: Durga Attili, University of Michigan Medical School, Pierluigi Barone, Corteva Agriscience, Raj Deepika Chauhan, Pairwise, and Michael Dame, University of Michigan Medical School |
In the last two decades the development of different site directed nucleases (e.g. ZFNs, TALENs and CRISPR-Cas) has provided tools to precisely and efficiently introduce a variety of genetic modifications in a broad spectrum of cell types and organisms. The most recent deployment of these programmable nucleases is represented by new applications including CRISPR gene drive, targeted induction of chromosomal rearrangements and diagnostic screening for genetic mutations and development of nutritious, flavorful new varieties of fruits and vegetables. In this session a comprehensive review and exciting recent developments in this area will be presented.
| 8:00 | Introduction (D. Attili, P. Barone, R.D. Chauhan, and M. Dame) | |
| 8:05 | PS-1 | Applications of and Considerations for CRISPR/Cas9 Mediated Gene Conversion in Rodents Kimberly Cooper, University of California, San Diego |
| 8:35 | PS-2 | Chromosome Engineering for Crop Improvement Sergei Svitashev, Corteva Agriscience |
| 9:05 | PS-3 | CRISPR-Powered Microchips: A Paradigm Shift for Amplification-free DNA Detection Kiana Aran, Keck Graduate Institute; Cardea Bio |
| 9:35 | PS-11 | Application of Genome Editing to Improve Nutritious, Fresh Produce Crops for the Consumer Ryan Rapp, Pairwise |
| 10:00 am – 10:30 am | Coffee Break | Pacific A |
| 10:00 am – 10:30 am | Public Policy Committee Meeting | Palm 8 |
| 10:30 am – 12:30 pm |
FRONTIERS IN GENE EDITING FOR CROP DEVELOPMENT* |
Pacific C&D |
| PLANT SYMPOSIUM | Conveners: Fredy Altpeter, University of Florida – IFAS, and Yiping Qi, University of Maryland-College Park |
| 10:30 | Introduction (F. Altpeter and Y Qi) | |
| 10:35 | P-1A | Engineering the “Engineers”: Efforts in the Biolistic Gun Improvement and Agrobacterium Genome Engineering Kan Wang, Iowa State University |
| 11:00 | P-2 | Developing Highly Efficient Base Editing and Prime Editing Tools in Plants Yiping Qi, University of Maryland – College Park |
| 11:25 | P-3 | Gene Editing in Plants Using Plant Viruses Savithramma Dinesh-Kumar, University of California – Davis |
| 11:50 | P-4 | Transforming the Food System with the Inari SEEDesignTM Platform Helene Berges, Inari Agriculture Inc. |
| 12:15 | Discussion | |
| P-1* | Synthetic Directed Evolution in Plants: Unlocking Trait Engineering and Improvement Magdy M. Mahfouz, KAUST, Saudi Arabia *Presentation will be available online only |
| 10:30 am – 12:30 pm |
ADVANCES IN DOUBLE HAPLOID TECHNOLOGY |
Pacific E |
| PLANT SYMPOSIUM | Conveners: Cliff Hunter, Consultant, and Charles L. Armstrong, Plastomics |
Doubled haploid technology is now a cornerstone of plant improvement programs for many of the world’s most important crops, including maize, wheat, oilseed rape, and a wide variety of vegetable / horticultural species. Significant, steady improvement has been made over the years in improving the rate of haploid induction, the identification and selection of haploids, and the efficiency of chromosome doubling and recovery of dihaploid seed. In recent years, important breakthroughs have been made in understanding the molecular basis of haploid induction. These include cloning of a major gene involved in haploid induction in maize and development of a novel chromosome elimination mechanism (modified CENH3), each of which has been demonstrated to work across species. Haploid induction has also been combined with gene editing to increase the speed with which edited traits can be fixed and evaluated in relevant germplasm. In this session, we will bring together representatives of several major seed companies to present selected highlights of recent work on dihaploid technology within their organizations.
| 10:30 | Introduction (C. Hunter and C. L. Armstrong) | |
| 10:35 | P-5 | Accelerating Precision Breeding through Double Haploids Lorena Moeller, Bayer Crop Science |
| 11:10 | P-6 | Breeding Technology & Discovery Progress at Syngenta Weiguo Liu, Syngenta Crop Protection, LLC |
| 11:45 | P-7 | Deciphering In Planta Haploidization In Maize Nathanaël Jacquier, Limagrain |
| 12:20 | Discussion |
| 10:30 am – 12:30 pm |
ORGANOID TECHNOLOGY |
Pacific H&I |
| ANIMAL SYMPOSIUM |
Conveners: Terry Riss, Promega Corporation, and Kristina Martinez-Guryn, Midwestern University |
The use of organoids has grown rapidly over the past several years. As a 3D culture model system, they have been demonstrated to more closely represent in vivo biology compared to cells cultured as a monolayer on plastic. The technology to produce organoids has improved greatly, resulting in protocols to generate organoids representing a variety of tissue types. There are many definitions of organoids; but a key element is they are derived from stem cells or organ progenitors that are driven to differentiate into several cell types that self-organize similar to the process in vivo. The speakers in this Symposia will present a general overview of organoids and provide examples of their generation and practical applications to advance our understanding of biology.
| 10:30 | Introduction (T. Riss) | |
| 10:35 | A-1 | Fitting Organoids into the Spectrum of Available 3D Culture Models Terry Riss, Promega Corporation |
| 11:00 | A-2 | Introducing a ‘Phase 0’ in Clinical Trials with Precise Organoid-based Disease Models Courtney Tindle, University of California, San Diego |
| 11:25 | A-3 | Initiation, Expansion, and Cryopreservation of Patient-derived Organoids from the Human Cancer Models Initiative James M. Clinton, American Type Culture Collection |
| 11:50 | A-4 | Brain Organoid Technology: a Versatile Tool to Study the Human Brain Francesca Puppo, University of California San Diego |
| 12:15 | Discussion |
| 11:00 am – 12:00 pm | SIVB/IAPB/Springer Meeting | Palm 7 |
| 12:30 pm – 1:30 pm | Exhibitors Refreshment Break | Pacific A |
| 12:30 pm – 1:30 pm | Membership Committee Meeting | Palm 8 |
| 12:30 pm – 1:30 pm | In Vitro – Plant Editorial Board Meeting | Palm 7 |
| 1:30 pm – 3:00 pm |
EXTRACELLULAR MATRIX AND TISSUE ENGINEERING |
Pacific H&I |
| ANIMAL SYMPOSIUM |
Conveners: Joshua Z. Gasiorowski, Midwestern University, and Michael K. Dame, University of Michigan Medical School |
Tissue engineering has had exciting clinical successes, but new translational breakthroughs in the field will be dependent upon researchers fully understanding the properties of endogenous extracellular matrices so they can design biologically relevant mimics. The extracellular matrix (ECM) is a three-dimensional proteinaceous meshwork that provides tensile strength and connective, anchoring material for all tissues. However, the ECM is also a rich source of signals that influence and control cell behaviors. The biochemical composition of collagens, laminins, and other fibrous proteins act as ligands for cell signaling receptors while the biophysical cues in the form of compliance, elasticity, and topography directly mediate independent cellular responses. This session will highlight recent advances in understanding the basic science properties of the extracellular matrix, in addition to discussing ECM mimics that are designed to serve as tissue engineering scaffolds for regenerative medicine.
| 1:30 | Introduction (J. Z. Gasioiorowski and M. K. Dame) | |
| 1:35 | A-5 | Beyond Cultrex BME: Ultimatrix and Other Matrices for Stem Cells and Organoids Kevin Flynn, Bio-Techne |
| 2:15 | A-6 | Dentin Extracellular Matrix: Biological and Mechanical Properties for Bioinspired Tissue Engineering Marcela R. O. Carrilho, Midwestern University |
| 2:55 | Discussion |
| 1:30 pm – 3:00 pm |
NOVEL DELIVERY TECHNOLOGIES – OVERCOMING BOTTLENECKS |
Pacific E |
| PLANT SYMPOSIUM | Convener: Gözde Demirer, California Institute of Technology |
| 1:30 | Introduction (G. Demirer) | |
| 1:35 | P-8 | Overcoming Bottlenecks that Prevent Efficient Foliar Uptake and Translocation of Polymer-based Nanoparticles in Crop Plants Gregory Lowry, Carnegie Mellon University |
| 2:00 | P-9 | Methods for the Generation of Genetically Engineered Dicotyledonous Plants Using Developmental Regulators Michael Maher, Invaio Sciences |
| 2:25 | P-10 | Nanomaterials for Plant Genetic Engineering Gözde Demirer, California Institute of Technology |
| 2:50 | Discussion |
| 1:30 pm – 3:00 pm |
PLANT GROWTH REGULATORS |
Pacific F&G |
| PLANT SYMPOSIUM | Convener: Geny Anthony, Corteva Agriscience, and Kristian Adamek, University of Guelph |
| 1:30 | Introduction (G. Anthony and K. Adamek) | |
| 1:35 | P-11 | Control Plant Growth by Modulating Auxin Biosynthesis and Degradation Yunde Zhao, University of California, San Diego |
| 2:15 | P-12 | ABA Receptors: Agonists, Antagonists, and Scaffolds for Biosensors Development Sean Cutler, University of California, Riverside |
| 2:55 | Discussion |
| 3:10 pm – 5:30 pm |
2022 IN VITRO BIOLOGY MEETING OPENING CEREMONY |
Pacific C&D |
| KEYNOTE SYMPOSIUM | Conveners: Mae Ciancio, Midwestern University, and Addy Alt-Holland, Tufts University |
| 3:10 | Welcome and Opening Remarks Mae Ciancio, Midwestern University Addy Alt-Holland, President, Society for In Vitro Biology |
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| 3:20 | ROBERT H. LAWRENCE, JR. KEYNOTE SYMPOSIUM | |
| Introduction (J. W. Harbell) | ||
| KS-1 | Advancing Cell Culture to Meet Scientific and Societal Needs Thomas Hartung, Director of the Center for Alternatives to Animal Testing (CAAT) and Inaugural Holder of the Doerenkamp-Zbinden Endowed Chair in Evidence-based Toxicology in the Department of Environmental Health Sciences at the Johns Hopkins Bloomberg School of Public Health |
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| 4:10 | Questions and Answers | |
| 4:20 | 2022 SOCIETY FOR IN VITRO BIOLOGY AWARDS CEREMONY Addy Alt-Holland, President, Society for In Vitro Biology |
|
| 2022 Lifetime Achievement Award Presentations | ||
| 4:25 | (Introduction by Sandra L. Schneider, Acknowledgement by Dr. Pomponi to follow) Shirley A. Pomponi, Harbor Branch Oceanographic Institute, Florida Atlantic University |
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| 4:40 | (Introduction by Wayne A. Parrott; Acknowledgement by Dr. Wang to follow) Kan Wang, Iowa State University |
|
| 4:55 | Acknowledgement of the 2020 and 2021 Lifetime Achievement Award Recipients Dwight T. Tomes, 2020 Lifetime Achievement Award Recipient Cynthia L. Goodman, 2021 Lifetime Achievement Award Recipient |
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| 5:10 | 2022 Fellow Award Recipients (Awards to be presented at Section Meetings) Mae Ciancio, Midwestern University Maria M. Jenderek, USDA Kolbrun Kristjansdottir, Midwestern University J. Pon Samuel, Corteva Agriscience |
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| 5:15 | 2022 Distinguished Service Award Presentations (Awards to be presented by Allan R. Wenck): Ad Hoc Webinar Committee Rakhi Chaturvedi, Indian Institute of Technology-Guwahati Ian Scott Curtis, Consultant Ahmad Al-Sayed Omar, University of Florida Brad L. Upham, Michigan State University |
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| Ad Hoc Social Engagement Committee Christopher Bagley, INARI Anissa Belfemi, Harvard University Sarbesh Das Dangol, Tribhuvan University |
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| Grant Support Sadanand A. Dhekney, University of Maryland, Eastern Shore |
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| Outgoing Board of Directors John W. Harbell, JHarbell Consulting Sukhpreet Sandhu, HMClause Kan Wang, Iowa State University |
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| 5:25 | Acknowledgement of the 2020 and 2021 Distinguished Service Award Presentations | |
| Barbara B. Doonan, New York Medical College John J. Finer, The Ohio State University Sukhpreet Sandhu, HMClause Dwight T. Tomes Brad L. Upham, Michigan State University Harold N. Trick, Kansas State University Kan Wang, Iowa State University Allan R. Wenck, Syngenta Crop Protection LLC |
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| 2021 Distinguished Service Award Recipients Addy Alt-Holland, Tufts University Pierluigi Barone, Corteva Agriscience Barbara B. Doonan,New York Medical College Raj Deepika Chauhan, Pairwise John W. Harbell, JHarbell Consulting, LLC Evan M. Hill, University of Michigan Max Jones, University of Guelph Angela Labrum, Bailey Nursery Kristina Martinez-Guryn, Midwestern University Alperen Ozturk, Nigde Omer Halisdemir University Wayne A. Parrott, University of Georgia M. Annie Saltarikos, Bayer U.S. – Crop Science Sukhpreet Sandhu, HMClause Marietta Wheaton Saunders, New Beginnings Management, Inc. Michele G. Schultz, New Beginnings Management, Inc. Brad L. Upham, Michigan State University |
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| 5:30 | Adjourn Group photo with Dr. Hartung and Student attendees |
| 5:30 pm – 6:30 pm | 2022 IN VITRO BIOLOGY MEETING OPENING CEREMONY RECEPTION | Pacific A |
| 6:30 pm – 7:30 pm | Poster Session Odd Poster Authors will be present. |
Pacific A |
| 7:30 pm – 9:30 pm |
STUDENT WORKSHOP: STATE-OF-THE-ART CELL IMAGING TECHNOLOGIES |
Pacific F&G/Pacific A |
| EDUCATION WORKSHOP | Mubeen ul Hasan, Nigde Omer Halisdemir University, Muneeb Hassan Hashmi, Nigde Omer Halisdemir University and Brad Upham, Michigan State University |
| 7:30 | Introduction (M ul Hasan and M. H. Hashmi) |
| 7:35 | Panel Discussion Panelists: Addy Alt-Holland, Tufts University Michael J. Fay, Midwestern University Brad L. Upham, Michigan State University Kan Wang, Iowa State University |
MONDAY, JUNE 6
| 7:00 am – 6:00 pm | Registration | Pacific Foyer |
| 7:00 am – 8:00 am | Plant Biotechnology Program Committee Meeting | Pacific F&G |
| 7:00 am – 8:00 am | Publications Committee Meeting | Palm 7 |
| 8:00 am – 10:00 am |
MICHAEL E. HORN EMERGING TECHNOLOGIES SYMPOSIUM: FUTURE FOODS |
Pacific C&D |
| PLENARY SYMPOSIUM |
Vivian R. Dayeh, University of Waterloo, Veena Veena, Donald Danforth Plant Science Center, and Sukhpreet Sandhu, HMClause |
Meats grown in a petridish, chocolate produced without cacao trees, and milk produced without ever stepping on a farm. Is this the future of the foods we eat? Increasing threats to world’s food production systems and the need to creating sustainable food products and reducing carbon emissions from agriculture, has been driving the growth of plant based and cell based foods. One of these emerging areas is cellular agriculture that relies on in vitro methods instead of whole plants or animals for food production. The Future Foods plenary session will present cutting edge research on alternative food sources including plant and animal cell cultures and how the technology will change the food system in the future. The speakers in this symposium will excite the audience on the future of alternative seafoods, chocolate, and milk products, and how these innovations will revolutionize agriculture.
| 8:00 | Announcement of the Michael E. Horn Emerging Technologies Symposium Addy Alt-Holland, President, Society for In Vitro Biology |
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| 8:05 | Introduction (V. R. Dayeh, V. Veena, and S. Sandhu) | |
| 8:10 | PS-4 | Protein Reimagined: Transforming the Global Food System Bruce Friedrich, The Good Food Institute |
| 8:35 | PS-5 | Plant-based and Cell-cultured Seafood for a More Sustainable Future and Healthier Ocean Brandon Chen, Finless Foods |
| 9:00 | PS-6 | Leveraging Bioprocessing and Fermentation Technologies for the Generation of Cell-based Milk and Bioactive Ingredients Aletta Schnitzler, Turtle Tree |
| 9:25 | PS-7 | The Fall of Specialty Monoculture Farming, and the Rise of Cell Cultured Foods Alan Perlstein, California Cultured |
| 9:50 | Discussion |
| 10:00 am – 10:30 am | Coffee Break | Pacific A |
| 10:00 am – 10:30 am | Awards Committee Meeting | Palm 7 |
| 10:30 am – 12:30 pm |
MOVING THE FIELD FORWARD: APPLICATION OF NEW TECHNOLOGIES IN ACADEMIC, INDUSTRY AND REGULATORY AGENCIES PERSPECTIVES |
Pacific H&I |
| ANIMAL SYMPOSIUM |
Conveners: Addy Alt-Holland, Tufts University, Kenneth Kandaras, International Foundation for Ethical Research, and John W. Harbell, JHarbell Consulting LLC |
Academic institutions are centers not only for learning but also for innovation that varies from highly exploratory framework to a more commercially aligned approach. With increasing frequency, academic researchers are interested in moving their ”basic” research program discoveries along the innovation pathway to commercial translation and development of new technologies for the benefit of humankind. The original basis of the biopharmaceutical industry, 3-D bioengineered tissue models, and organs-on-a-chip technologies are only a few examples of this transition, which started as basic science questions that grew into applied technologies. However, although numerous invention disclosures, intellectual property, and patents are generated in academic institutions, they are not always translated to licenses and spin-outs of successful start-up companies. The translation of research to commercialized innovations is often hindered by multiple factors, such as professional motivations and interest, knowledge of the innovation process, and expectations of outcomes. Moreover, the gap from conceiving research-based ideas in the academic arena to their translation into industry-based, innovative and applicable products in the healthcare settings consists of multiple barriers. These span from inadequate design of research programs, to insufficient marketing of the ideas, to technical development and manufacturing (including the reliability, intended objective efficacy and predictive capacity of the innovative end product), to complex regulatory procedures, approvals and policies that can influence the acceptance and commercial viability of new technologies. As moving from research idea towards a commercial product is a complex process, innovation in healthcare requires multidisciplinary teams that are based on engaged stakeholders from both academic and industry. Do you have a technology that you feel is worth commercializing? This symposium will examine the factors that influence moving the innovation filed forward through the experiences of three speakers from organizations that are bringing new technologies to the scientific academic, industrial, and regulatory communities. Our speakers will discuss their experience, challenges and perspectives on the translation of research to commercialized innovations, how academic motivations and work practices can shape successful research translation, as well as the characteristics and expectations involved in healthcare innovation in industry.
| 10:30 | Introduction (J. W. Harbell, K. Kandaras and A. Alt-Holland) | |
| 10:35 | A-7 | From Assay to Products – Lessons Learned Thomas Hartung, Center for Alternatives to Animal Testing |
| 11:10 | A-8 | From Lab to Product: Commercialization Paths for Medical Diagnostic Tests Hadley Sikes, Massachusetts Institute of Technology |
| 11:45 | A-9 | From Academia to Industry – the Importance of Good Laboratory Practices Guidelines for Developing Consistent Study Execution John W. Harbell, JHarbell Consulting LLC |
| 12:20 | Discussion |
| 10:30 AM –12:10 PM |
PLANT BIOTECHNOLOGY POST-DOCTORAL ORAL PRESENTATION COMPETITION |
Pacific E |
| PLANT CONTRIBUTED PAPER SESSION |
Moderator: Ahmad Al-Sayed Omar, University of Florida | |
| Panel of Plant Biotechnology Experts Evaluating the Contestants: Pal Maliga, Rutgers University, Carlos M. Hernandez-Garcia, CTC Gemonics, and Terrence Frett, Sun World Innovations |
To support the Society’s vision to encourage education and scientific informational exchange and recognize outstanding post docs, the Plant Biotechnology Section is pleased to announce the 2022 Post-Doctoral Oral Presentation Competition. Competition finalists were selected based on the quality of the abstracts. A panel of judges will evaluate the presentations at the meeting. Criteria for the evaluation include experimental design, data analysis, proper interpretation of the results, originality of the study, technical difficulty, appearance and ability of the Post-Doctoral candidate to present it. Winners will be presented with a certificate and a cash award at the meeting.
| 10:30 | P-1000 | Field Evaluation of Metabolically Engineered Energycane for Hyperaccumulation of Triacylglycerol Viet Dang Cao, DOE Center for Advanced Bioenergy and Bioproducts Innovation, Baskaran Kannan, Shelby Korynta, Hui Liu, Steve Long, John Shanklin, and Fredy Altpeter |
| 10:50 | P-1001 | Author is unable to attend. |
| 11:10 | P-1002 | Optimizing CRISPR/Cas9-mediated Genome Editing in Vitis Papaiah Sardaru, University of Maryland Eastern Shore C. Jackson, A. Junior, B. Khatabi, X. Dai, Y. Zhao and S. A. Dhekney |
| 11:30 | P-1003 | Comparison of Constitutive Promoters for Spatial Regulation of Transgenes in Different Vegetative Tissues of Sugarcane Rajesh Yarra, University of Florida, Evelyn Zuniga-Soto, and Fredy Altpeter |
| 11:50 | P-1004 | Author is unable to attend. |
| 10:30 am – 12:30 pm |
PLANT BIOTECHNOLOGY STUDENT ORAL PRESENTATION COMPETITION |
Pacific C&D |
| PLANT CONTRIBUTED PAPER SESSION |
Moderator: Alex da Silva Conceicao, Calyxt | |
| Panel of Plant Biotechnology Experts Evaluating the Contestants: Max Jones, University of Guelph, Satya Swathi Nadakuduti, University of Florida, Jeff Beringer, Inari Agriculture, and Uyen Cao Chu, Corteva Agriscience |
To support the Society’s vision to encourage education and scientific informational exchange, the Plant Biotechnology Section is pleased to announce the 2022 Student Oral Presentation Competition. Student competition finalists were selected based on the quality of the abstracts. A panel of judges will evaluate the presentations at the meeting. Criteria for the evaluation include experimental design, data analysis, proper interpretation of the results, originality of the study, technical difficulty, appearance and ability of the student candidate to present it. Winners will be presented with a certificate and a cash award at the meeting.
| 10:30 | P-1005 | Highly Conserved sgRNA Target Sequences Support Cas9-mediated Mutagenesis of LIGULELESS1 in Both Sorghum and Sugarcane Eleanor J. Brant, University of Florida, Ayman Eid, Mehmet Cengiz Baloglu, Aalap Parikh, and Fredy Altpeter |
| 10:50 | P-1006 | Knock-down of Vital Gene(s) of Tuta absoluta (Meyrick) (Lepidoptra: Gelechiidae) Using in Planta RNAi M. H. Hashmi, Niğde Ömer Halisdemir University, U. Demirel, and A. Bakhsh |
| 11:10 | P-2000 | Comparative Assessment of Antioxidant Activity of Prenylated Stilbenoid-Rich Extracts from Elicited Hairy Root Cultures of Three Different Cultivar of Peanut G. Gajurel, Arkansas Biosciences Institute, R. Hasan, and F. Medina-Bolivar |
| 11:30 | P-1008 | Efficient, Multi-allelic Editing for the Genetic Improvement of Bahiagrass (Paspalum notatum Flüggé) David May, University of Florida, Jennifer Gilby, Sara Sanchez, and Fredy Altpeter |
| 11:50 | P-1009 | Targeted Mutagenesis of Vacuolar H+ Translocating Pyrophosphatase (V-PPase) Promoter Limits Sucrose Formation and Disturbs Cytosolic pH During Germination in Rice D. Dharwadker, University of Arkansas, P. J. I. Gann, C. Maurya, S. Nandy, S. Zhao and V. Srivastava |
| 12:10 | P-1010 | Deletion in the GATA Promoter Element of Vacuolar H+ Translocating Pyrophosphatase (V-PPase) by CRISPR/Cas9 Reduces Chalkiness in Rice Peter James Icalia Gann, University of Arkansas, Dominic Dharwadker, Chandan Mayura, Soumen Nandy, Shan Zao, and Vibha Srivastava |
| 12:30 pm – 1:30 pm | Long Range Planning Committee Meeting | Palm 7 |
| 12:30 pm – 1:30 pm | Refreshment Break | Pacific A |
| 12:30 pm – 1:30 pm |
STUDENT NETWORKING LUNCHEON: RESEARCH PROPOSAL AND GRANT WRITING |
Pacific F&G |
| EDUCATION WORKSHOP | Conveners: Mubeen ul Hasan, Nigde Omer Halisdemir University, Muneeb Hassan Hashmi, Nigde Omer Halisdemir University, and Brad L. Upham, Michigan State University |
Have you been looking for how to write a successful research proposal and how to get funding for your research as a graduate student? Have you been searching for how to find the right funding agency for your research or even thought about writing a proposal that will be accepted by your funding agency or your supervisor? Or are you confused about how to target the key areas of a topic that will make your research proposal interesting for the jury and funding agency? If that’s the case, this is your golden opportunity! Come to the student networking luncheon to find all the answers you have been waiting for. We are putting together a panel of researchers that will answer all of your questions and will help alleviate any confusion. If you are a new Master’s or Ph.D. student who needs to write a proposal, then researchers will be available to assist you and provide you with information about how to find the perfect topic for your research as well as all the material that you need to write a proposal. If you are a student and you need to find a public or private funding agency to fund your research, our professionals will help you understand the requirements of the various funding agencies. Similarly, if you want to apply for a scholarship to study abroad and your scholarship requires a strong research proposal, this panel will tell you how they chose students for these positions which can help you get answers to your questions. Professionals will also share their academic experiences and discuss how they coped while pursuing their graduate degrees. This workshop may also introduce you to your new research mentor, supervisor, collaborator, and/or external internship opportunity.
| 12:30 | Introduction (M ul Hasan and M. H. Hashmi) |
| 12:35 | Discussion |
| 1:30 pm – 2:30 pm |
IN VITRO ANIMAL CELL SCIENCES STUDENT AND POST DOCTORAL ORAL PRESENTATION COMPETITION |
Pacific H&I |
| ANIMAL CONTRIBUTED PAPER SESSION |
Moderators: Addy Alt-Holland, Tufts University, and Kolla Kristjansdottir, Midwestern University | |
| Panel of In Vitro Animal Cell Sciences Experts Evaluating the Contestants: Addy Alt-Holland, Tufts University, Kolla Kristjansdottir, Midwestern University, John W. Harbell, JHarbell Consulting, LLC, Barbara Doonan, New York Medical College, Michael J. Fay, Midwestern University, Brad L. Upham, Michigan State University, Michael Dame, University of Michigan, Mae Ciancio, Midwestern University, Anissa Belfetmi, Harvard University, and Zoe Xiaofang Zhu, Tufts University |
| 1:30 | A-1000 | Habitat Restoration and Production of Bioactive Compounds from 3D Sponge Cell Cultures Megan Conkling, Harbor Branch Oceanographic Institute, Elizabeth Urban-Gedamke, Amy E. Wright, and Shirley A. Pomponi |
| 1:50 | A-1001 | Assessing the Apoptosis Effect of Prenylated Stilbenoids Combined with Paclitaxel in Triple-negative Breast Cancer Cells Sepideh Mohammadhosseinpour, Arkansas State University, A. Weaver, L-C. Ho, and F. Medina-Bolivar |
| 2:10 | A-1002 | A Flavonoid-rich Extract of Moringa oleifera (Moringaceae) Leaf Cultivated in Brazil Inhibited Inflammatory Mediators in Lipopolysaccharide-treated Macrophages Larissa Marina Pereira Silva, North Carolina State University, Jade Schlam, Leandro Ferreira, Silvana Zucolotto, and Debora Esposito |
| 1:30 pm – 2:30 pm |
BIOTECHNOLOGY |
Pacific A |
| PLANT INTERACTIVE POSTER SESSION |
Moderator: Baskaran Kannan, University of Florida |
| P-2000 | Comparative Assessment of Antioxidant Activity of Prenylated Stilbenoid-Rich Extracts from Elicited Hairy Root Cultures of Three Different Cultivar of Peanut G. Gajurel, Arkansas Biosciences Institute, R. Hasan, and F. Medina-Bolivar |
| P-2001 | In Vitro Selection for Fusaric Acid Resistant Egyptian Cumin Plants (Cuminum cyminum L.). Hossam M. Kamel, Minia University, Kasem Z. Ahmed, and Mohamed K. A. Aly |
| P-2002 | RNAi Mediated Suppression of Flowering in Energycane to Enhance Biocontainment. Baskaran Kannan, University of Florida, Thaibinhduong Nguyen, Niki Koukoulidis, Qasim Ali, Frico Situmeang, and Fredy Altpeter |
| 1:30 pm – 2:30 pm |
BIOTECHNOLOGY, GENOMICS, AND PLANT PHYSIOLOGY |
Pacific A |
| PLANT INTERACTIVE POSTER SESSION |
Moderator: Savanah Senn, Los Angeles Pierce College |
| P-3000 | A Rapid and Simplified Transformation and Genome Editing Method for Maize Inbred B104 Using Agrobacterium Ternary Vector System and Immature Embryos Minjeong Kang, Iowa State University, Keunsub Lee, Todd Finley, Hal Chappell, Veena Veena, and Kan Wang |
| P-3001 | CRISPR/Cas9 Mediated Cell Wall Engineering of Plant Cells for Enhanced Recombinant Protein Production Uddhab Karki, Arkansas State University, Hong Fang, and Jianfeng Xu |
| P-3002 | Post-wildfire Soil Microbiome Analysis of eDNA from the Angeles National Forest Savanah Senn, Los Angeles Pierce College, Gerald Presley, and Sharmodeep Bhattacharyya |
| P-3003 | Advanced Plant Reporter Genes for Transient Expression Nathan Vorodi, Pennsylvania State University, Natalie Thompson, David Samson, Rekha Kandaswamy, Vijay Sheri, Aliya F. Anwar, Michael Ream, Anna Filipkowski, Maia Clipsham, Sairam Rudrabhatla, and Wayne R. Curtis |
| P-3004 | Establishment of an Axenic Whitefly Colony for In Vitro Virus Transmission Studies Wayne R. Curtis, The Pennsylvania State University, Natalie Thompson, David Krum, Yun-Ru Chen, Mariela Torres, Marena Trauger, Dalton Strike, Zach Weston, April Hile, and Jane Polston |
| P-3005 | Genome-wide Analysis of Alpha-amylase Gene Family in Major Cereal Crops Shabda Verma, Punjab Agricultural University, Kanwardeep S. Rawale, Navraj Kaur Sarao, Johar Singh Saini, Gagandeep Singla, and Kulvinder S Gill |
| 2:30 pm – 3:30 pm | Poster Session Even Poster Authors will be present. |
Pacific A |
| 3:30 pm – 5:00 pm |
ALTERNATIVE APPROACHES TO PLASTID ENGINEERING |
Pacific E |
| PLANT SYMPOSIUM | Convener: Jeffrey Staub, Plastomics |
| 3:30 | Introduction (J. Staub) | |
| 3:35 | P-13 | Agrobacterium-mediated Transformation of the Plastid GenomePal Maliga, Rutgers University |
| 4:00 | P-14 | Modification of the Plastid Genome Using Base Editing Shin-Ichi Arimura, The University of Tokyo |
| 4:25 | P-15 | Targeted Delivery of Plasmid DNA to Chloroplasts by Nanomaterials Juan Pablo Giraldo, University of California Riverside |
| 4:50 | Discussion |
| 3:30 pm – 5:00 pm |
NEW APPROACHES TO VACCINE DEVELOPMENT |
Pacific C&D |
| JOINT SYMPOSIUM | Conveners: Julie Swartzendruber, Midwestern University, Barbara B. Doonan, New York Medical College, and J. Pon Samuel, Corteva Agriscience |
This joint symposium will focus on new developments and issues related to the use of vaccines. The rapid development and deployment of the COVID-19 vaccine has generated a lot of interest around how vaccines are developed. This session will invite expert faculty to discuss the latest information on vaccines including the future of vaccine development and surveillance.
| 3:30 | Introduction (J. Swartzendruber, B. B. Doonan and J. P. Samuel) | |
| 3:35 | J-1 | Plant-made Vaccines and Therapeutics for COVID-19 Henry Daniell, University of Pennsylvania |
| 4:15 | J-2 | Biotechnology for the Next Generation of Veterinary Antiviral Vaccines Hiep Vu, Nebraska Center for Virology |
| 4:55 | Discussion |
| 3:30 pm – 5:00 pm |
NON-COMPETITIVE STUDENT ORAL PRESENTATIONS |
Pacific F&G |
| EDUCATION SYMPOSIUM | Moderators: Mubeen ul Hasan, Nigde Omer Halisdemir University, and Muneeb Hassan Hashmi, Nigde Omer Halisdemir University |
| 3:30 | Introduction (M. ul Hasan, and M.H. Hashmi) | |
| 3:35 | A-2007 | Novel Mammalian Fibroblast Cell Culture Media Technique for Ultraviolet Cell Reduction J. Hoeger, University of Dubuque |
| 3:55 | A-3013 | Sex linked Aberrant Behavior and Hippocampal Gene Expression in Egr1 Conditional Knockout Mice C. Swilley, Virginia-Maryland College of Veterinary Medicine, and H. Xie |
| 4:15 | P-3013 | Inducible Expression for Acceleration of Design-Build-Test-Learn Cycles in the Metabolic Engineering of Oilcane Moni Qiande, University of Florida – IFAS, Dang Viet Cao, Hui Liu, John Shanklin, and Fredy Altpeter |
| 4:35 | A-3007 | Cell Cycle Analysis of Vismodegib-treated Human Basal Cell Carcinoma Cells In-vitro B. Senfi, Tufts University School of Dental Medicine, T. Mendez, M. Evers, S. Pagni, J. Cowan, and A. Alt-Holland |
| 4:55 | Discussion |
| 5:00 pm – 6:00 pm |
IN VITRO ANIMAL CELL SCIENCES CONTRIBUTED PAPERS |
Pacific H&I |
| ANIMAL CONTRIBUTED PAPER SESSION |
Moderator: Mae Ciancio, Midwestern University |
| 5:00 | A-1003 | Successful Scale Up of Marine Sponge Cell Cultures in 3D Bioprinted Hydrogel Microdroplets Elizabeth H. Urban-Gedamke, Harbor Branch Oceanographic Institute, Megan Conkling, and Shirley A. Pomponi |
| 5:12 | A-1004 | Antimicrobial Studies of 1,3-Diphenylpyrazole-derived Anilines Against Methicillin-resistant Staphylococcus aureus Hansa Raj KC, Arkansas State University, David Gilmore, and Mohammad A. Alam |
| 5:24 | A-1005 | Moderate Heat Assisted Electrotransfer as an Effective Means for Delivering Molecules to Cells and Tissue Richard Heller, University of South Florida, Jody Synowiec, Samantha Mannarino, Julie Singh, and Guilan Shi |
| 5:36 | A-1006 | LncRNA APDC, a Long Non-coding RNA, Plays Important Regulatory Roles in Metabolism of Bone and Adipose Tissues Zoe Zhu, Tufts University School of Dental Medicine, Yao Liu, Elissa K. Zboinski and Jake Chen |
| 5:48 | A-1007 | Developing Cell Lines from Fish and Shellfish – Cellular Aquaculture Invitromatics Lucy E. J. Lee, University of the Fraser Valley |
| 5:00 pm – 6:00 pm |
BIOTECHNOLOGY |
Pacific C&D |
| PLANT CONTRIBUTED PAPER SESSION |
Moderator: Nagesh Sardesai, Corteva Agriscience |
| 5:00 | P-1011 | Somatic Embryogenesis in Cell Suspension Cultures of Sweetpotato (Ipomoea batatas) Kedong Da, North Carolina State University, H. Leng, W. Liu, and G. C. Yencho |
| 5:15 | P-1012 | Agrobacterium-mediated Genetic Transformation of Rose Embryogenic Cell Suspension Cultures Kedong Da, North Carolina State University, H. Leng, D. Harmon, A. Nelson, N. Maren, W. Liu, and T. Ranney |
| 5:30 | P-1013 | Identification and Validation of an Embryogenic Tissue Culture Response Gene from Maize Inbred Line A188 That Induces Embryogenic Callus Formation and Somatic Embryogenesis in Recalcitrant Maize Inbred B73 Frank McFarland, University of Wisconsin, Ray Collier, Kaitlyn Vondracek, Dalia Macias Martinez, and Heidi Kaeppler |
| 5:45 | P-1014 | Reproduction Control Tools for Eucalyptus: Knockout of Flowering and Meiosis Genes Using CRISPR/Cas9 Mitigates Concerns about Gene Dispersal While Maintaining Normal Vegetative Development Steven H, Strauss, Oregon State University, Michael F. Nagle, Surbhi Nahata, Bahiya Zahl, Alexa Niño de Rivera, Xavier V. Tacker, Estefania Elorriaga, Cathleen Ma, Greg Goralogia, Amy Klocko, Ellis Kline, and Michael Gordon |
| 5:00 pm – 6:00 pm |
BIOTECHNOLOGY, GENOME EDITING, AND GENETIC ENGINEERING |
Pacific C&D |
| PLANT CONTRIBUTED PAPER SESSION |
Moderator: Chhandak Basu, California State University, Northridge |
| 5:00 | P-1015 | Molecular Physiological Responses of Paulownia and Turmeric Under Various Abiotic Stress Conditions C. Basu, California State University, Northridge, M. Chaires, K. Cooper, D. Gupta, N. Joshee, N. Katiyar, S. Pakala, N. Ramadoss, B. Bharadwaj, and K. Musaev |
| 5:15 | P-1016 | DNA-Free Genome Editing in Hexaploid Sweetpotato Directed by Preassembled CRISPR-Cas9 Ribonucleoprotein Complexes Adrianne Brown, Tuskegee University Plant Biotechnology and Genomics Research Laboratory, M. Egnin, F. Bukari, D. Mortley, C. Bonsi, O. Idehen, D. Alexander, and G. Bernard |
| 5:30 | P-1017 | Constitutive Expression of a miR169 Gene Alters Plant Development and Enhances Drought and Salt Tolerance in Transgenic Creeping Bentgrass Xiaotong Chen, Clemson University, Jason Yeung, Andrew Fiorentino, Qian Hu, Morgan Kuess, and Hong Luo |
| 5:45 | P-1018 | T-DNA-Free Gene Editing Through Transient Suppression of the POLQ Gene in Plants Heqiang Huo, University of Florida, Guiluan Wang, and Zhanao Deng |
| 6:00 pm – 7:30 pm | In Vitro Animal Cell Sciences Section Business Meeting | Pacific H&I |
| 6:00 pm – 7:30 pm | Plant Biotechnology Section Business Meeting | Pacific F&G |
| 7:30 pm – 10:00 pm | Joint Sections’ Social | Pacific Foyer and Flamingo Lawn |
| 8:30 pm – 10:00 pm |
PUBLIC-PRIVATE COLLABORATIONS THAT SPUR INNOVATION WORKSHOP |
Pacific C&D |
| PLANT WORKSHOP | Moderators: Sukhpreet Sandhu, HMClause, and Kathy Munkvold, Corteva Agriscience |
| 8:30 | Introduction (S. Sandhu and K. Munkvold) |
| 8:35 | Panel Discussion Panelists: Sukhpreet Sandhu, HMClause Kathy Munkvold, Corteva Agriscience Barbara Mazur, Pontifax AgTech David D. Songstad, Songstad Consulting LLC Brigitte Weston, Gates Ag One Joyce Van Eck, Boyce Thompson Institute |
TUESDAY, JUNE 7
| 7:00 am – 5:00 pm | Registration | Pacific Foyer |
| 7:00 am – 8:00 am | In Vitro – Animal Editorial Board Meeting | Palm 7 |
| 7:00 am – 8:00 am | Student Affairs Breakfast | Pacific F&G |
| 8:00 am – 10:00 am |
ARTIFICIAL INTELLIGENCE AND MACHINE LEARNING IN PLANT AND ANIMAL SCIENCES |
Pacific C&D |
| PLENARY SYMPOSIUM | Conveners: Mae Ciancio, Midwestern University, M. Annie Saltarikos, Bayer U.S. – Crop Science, and Evan M. Hill, University of Michigan Student Convener: Babak Senfi, Tufts University |
Employing emergent technologies to optimize both human and plant health are important to the progress of our society and the world. Artificial intelligence and machine learning are changing the way scientists diagnose disease, identify effective drug modalities, analyze large datasets, and report outcomes. In this session, leaders in the field of AI and machine learning with specific interests in cancer, pharmacology, and crop sustainability will discuss current and ongoing efforts in this very exciting and dynamic field of investigation.
| 8:00 | PS-8 | The State of the Art of Artificial Intelligence in Healthcare Aziz Nazha, Sidney Kimmel Cancer Center, Thomas Jefferson University |
| 8:40 | PS-9 | Transfer Learning in Plant Biology William Link, Bayer U.S. – Crop Science |
| 9:20 | PS-10 | TBD Gabriel A. Silva, University of California San Diego |
| 10:00 am – 10:30 am | Coffee Break | Pacific A |
| 10:00 am – 10:30 am | Education Committee Meeting | Palm 7 |
| 10:30 am | Closing of the Mission Bay Silent Auction | Pacific A |
| 10:30 am – 12:30 pm |
ADVANCES IN CANNABIS BIOTECHNOLOGY |
Pacific C&D |
| PLANT SYMPOSIUM | Convener: Max Jones, University of Guelph |
The application of tissue culture and biotechnology is becoming a vital component of Cannabis businesses, especially as the market grows globally. Recent studies have found that plant pathogens have led to billions of dollars in damages to the Cannabis industry in North America, and advances in Cannabis Biotechnology have been identified as a potential solution to some of these problems. Additionally, the industry has had to innovate and draw from research and academia to fulfill the skyrocketing demand for healthy plants. This session brings together industry and academic researchers who are actively attempting to understand the challenges faced by current Cannabis businesses and the gaps that remain in our understanding of the horticulture and biology of Cannabis in the modern context. We hope to present information on treating pathogens (especially hop latent viroid), advances to Cannabis tissue culture techniques and advances in acclimatization of lab-grown plants back to greenhouses to be ready for cultivation.
| 10:30 | Introduction (M. Jones) | |
| 10:35 | P-16 | Controlled Ex Vitro Environments to Root Cannabis Microcuttings Following Multiple Harvest in a Hedging System Jeffrey Adelberg, Clemson University |
| 11:00 | P-17 | TBD Hope Jones, Emergent Crop Sciences (ECS) |
| 11:25 | P-18 | Advances in Cannabis Biotechnology- innovations in Cleaning, Screening, and Germplasm Maintenance Chris Leavitt, Node Labs |
| 11:50 | P-19 | Cannabis Virology in 2022 – Diagnostic and Remediation Options Joseph Ramahi, Apikal Biotek |
| 12:15 | Discussion |
| 10:30 am – 12:30 pm |
THE ART AND SCIENCE OF CELL IMAGING |
Pacific H&I |
| ANIMAL SYMPOSIUM | Conveners: Brad L. Upham, Michigan State University, Debora Esposito, North Carolina State University, and Cynthia L. Goodman, USDA/ARS/BCIRL Student Conveners: Muneeb Hassan Hashmi, Nigde Omer Halisdemir University and Mubeen ul Hasan, Nigde Omer Halisdemir University |
| 10:30 | Introduction (B. L. Upham, C. L. Goodman, M. H. Hashimi, and M. ul Hasan) | |
| 10:40 | A-10 | Discovery and Development of Wound Healing Agents from Natural Sources- Brassinosteroids and Beyond Debora Esposito, North Carolina State University |
| 11:15 | A-11 | Illuminating the Biochemical Activity Architecture of the Cell Jin Zhang, University of California, San Diego |
| 11:50 | A-12 | Image Cytometry–based Method Used to Measure Cellular and Subcellular Biology for High-content Screening Nicholas Radio, Thermo Fisher Scientific |
| 12:25 | Discussion |
| 10:30 am – 12:30 pm |
MODEL SYSTEMS FOR DEVELOPING CRISPR/CAS TECHNOLOGY IN PLANTS |
Pacific E |
| PLANT SYMPOSIUM | Conveners: Shubha Subbarao, Bayer U. S. – Crop Science, and Jeff Beringer, Inari Agriculture |
Genome editing has the potential to speed up the pace of plant breeding and trait development. As researchers seek to gain a greater understanding of CRISPR/Cas and related technologies the need to rapidly test the effectiveness of a multitude of editing concepts has become a pressing need. Rapid assay systems provide the opportunity to evaluate large numbers of constructs without engaging in the lengthy regeneration of mature plants, thereby helping manage costs and resources effectively.
| 10:30 | Introduction (S. Subbarao and J. Beringer) | |
| 10:35 | P_20 | Application of Plant Rapid Expression Systems in Accelerating Gene Expression and Gene Editing Research Peizhen Yang, Bayer Crop Science |
| 11:00 | P-21 | Pre-selection of Guide RNAs Enables Efficient Genome Editing in Planta Yan Liang, Joint Genome Institute |
| 11:25 | P-22 | CoverCress – A Novel Oilseed Winter Crop with Canola-like Composition That Helps Sequester Carbon and Prevent Soil Erosion Tim Ulmasov, CoverCress Inc. |
| 11:50 | P-23 | Transforming Blackberry from Niche Crop to High Throughput Platform for Precision Breeding Technologies Raj Deepika Chauhan, Pairwise |
| 12:15 | Discussion |
| 12:30 pm | Announcement of the Mission Bay Silent Auction Winners | Pacific Foyer |
| 12:30 pm – 1:30 pm | 2023 Program Planning and Development Committee Meeting | Palm 7 |
| 1:30 pm – 2:30 pm |
GENETIC ENGINEERING AND METABOLIC ENGINEERING |
Pacific A |
| PLANT INTERACTIVE POSTER SESSION |
Moderator: Heqiang Huo, University of Florida |
| P-3012 | Increased Engineering and Editing Efficiency of Sorghum bicolor Using Morphogene-assisted Transformation Kiflom Aregawi, University of California, Berkeley, Jianqiang Shen, Grady Pierroz, Manoj K. Sharma, Jeffery Dahlberg, Judith Owiti, and Peggy G. Lemaux |
| P-3013 | Inducible Expression for Acceleration of Design-Build-Test-Learn Cycles in the Metabolic Engineering of Oilcane Moni Qiande, University of Florida – IFAS, Dang Viet Cao, Hui Liu, John Shanklin, and Fredy Altpeter |
| P-3014 | Application of Developmental Regulators to Improve In-Planta or In Vitro Transformation in Plants Heqiang Huo, University of Florida, Zhaoyuan Lian, Chi Dinh Nguyen, Jianjun Chen, Sandra Wilson, and Peggy Ozias-Akins |
| P-3015 | Assessment of Maize Lethal Necrosis Disease Severity at Various Stages of Five cv. of Maize Crop M. Qais, Georg-August-University, and A. Abbas |
| P-3016 | Protein Engineering in Chlamydomonas reinhardtii for Improved Production of Recombinant Proteins Corbin England, Arkansas State University, and Jianfeng Xu |
| P-3017 | Development of an In Vitro Regeneration and Transformation System for Hop (Humulus lupulus) Christopher J. Willig, Oregon State University, Michele S. Wiseman, John A. Henning, David H. Gent, and Steven H. Straus |
| 1:30 pm – 2:30 pm |
IN VITRO ANIMAL CELL SCIENCES |
Pacific A |
| ANIMAL INTERACTIVE POSTER SESSION |
Moderator: Vivian Dayeh, University of Waterloo |
| A-2000 | Signaling Pathways of Modulated Electro-hyperthermia Induced Tumor Cell Killing Enikő Major, Semmelweis University, Andrea Balogh, Balázs Besztercei, Anett Benedek, and Zoltán Benyó |
| A-2001 | Toxic Assessment of Environmental Contaminants Using a Novel In Vitro High Throughput Bioassay Screening System of Gap Junctional Intercellular Communication Brad L. Upham, Michigan State University, Lizbeth Lockwood, Jamie Liebold, Joo Hye Yeo, Jinu Lee, Alison K. Bauer, Erika Lisabeth, and Richard Neubig |
| A-2002 | Effects of Sodium Butyrate and D- and L-lactate on Rainbow Trout Intestinal Epithelial Cells Daylan Pritchard, University of the Fraser Valley, Eryn Braley, Patrick G. Pumputis, Vivian R. Dayeh, Niels C. Bols, and Lucy E. J. Lee |
| A-2003 | Explant Cultures of Rainbow Trout Olfactory Rosettes (ORs): Cell Outgrowth, Degeneration, Propagation, and Characterization R. Goldbach, University of the Fraser Valley, J. B. Lee, N. C. Bols, and L. E. J. Lee |
| A-3000 | The Establishment of a Sponge Cell Hybridoma Cassady Dougan, Florida Atlantic University, Megan Conkling, Peter J. McCarthy, Amy E. Wright, and Shirley A. Pomponi |
| A-3001 | ECEL1, PIEZO1 and NAV2 Genes Are Altered in the Enhanced and Directed Axonal Growth in Dorsal Root Ganglia Grown Ex Vivo on Nano-scale Grooved Topographical Surfaces Julie Ellen Tamayo, Midwestern University, Kelly Keeler, Joshua Z. Gasiorowski, Michele Fornaro, and Kolla Kristjansdottir |
| 1:30 pm – 2:30 pm |
MICROPROPAGATION/MEDICINAL PLANTS |
Pacific A |
| PLANT INTERACTIVE POSTER SESSION |
Moderator: Kristian Adamek, University of Guelph |
| P-3006 | Mutation Rates in Micropropagated Cannabis sativa Detected Through Genotyping by Sequencing, Are SSRs Antiquated? Kristian Adamek, University of Guelph, Andrew Maxwell Phineas Jones, and Davoud Torkamaneh |
| P-3007 | Direct Field-to-Lab Introduction of Shoot Tips and Nodal Sections from Wild Nuttal’s Scrub Oak (Quercus dumosa) for the Purpose of Ex Situ Conservation Via Micropropagation J. Ree, San Diego Zoo Wildlife Alliance, and C. Powell |
| P-3008 | Clonal Propagation of Avocado (Persea americana) from Adult Trees: Establishment In Vitro J. Tin, The Huntington Library, Art Museum, and Botanical Gardens, and R. Folgado |
| P-3009 | Isolation and Characterization of Two Novel Chryseobacterium sp. Genomotypes from the Rootzone of the Medicinal Plant Datura inoxia by Whole Genome Sequencing Savanah Senn, Los Angeles Pierce College, Kelly Pangell, Maryam Saraylou, Adrianna Bowerman, Karu Smith, and Ryan O’halloran |
| P-3010 | Moringa oleifera Leaf Extract as a Potential Plant Growth Stimulant to Enhance Salt Stress Tolerance in Wheat Talaat Ahmed, Qatar University, Mustafa Elshiekh, and Muhammad Fasih Khalid |
| 2:30 pm – 3:30 pm | Poster Session Odd Poster Authors will present |
Pacific A |
| 3:30 pm – 4:00 pm | Poster Breakdown and Removal | Pacific A |
| 3:30 pm – 5:00 pm |
GENETIC TRANSFORMATION AND REGENERATION OF RECALCITRANT SPECIES (I.E., FRUIT TREES, ORPHAN CROPS), CHALLENGES AND WAY FORWARD |
Pacific C&D |
| PLANT SYMPOSIUM | Conveners: Carlos M. Hernandez-Garcia, CTC Genomics, Juan Debernardi, University of California, Davis, and Pamela Vogel, Pairwise |
| 3:30 | Introduction (C. M. Hernandez-Garcia, J. Debernardi, and P. Vogel) | |
| 3:35 | P-24 | Of Media and Miracles: Successes and Frustrations in the Search for Efficient Regeneration and Transformation Methods for Trees and Crops Steven Strauss, Oregon State University |
| 4:00 | P-25 | A CRISPR-combo Approach for Speed Breeding and Regeneration of Genome-edited Plants Yiping Qi, University of Maryland-College Park |
| 4:25 | P-26 | Efficient Plant Regeneration from Protoplasts Isolated from Multiple Grape Genotypes and Demonstration of PEG-mediated Gene Editing Using CRISPR/Cas9 David Tricoli, University of California-Davis |
| 4:50 | Discussion |
| 3:30 pm – 5:00 pm |
IN VITRO TECHNOLOGIES FOR PLANT CONSERVATION AND GENE BANKING |
Pacific E |
| PLANT SYMPOSIUM | Convener: Valerie C. Pence, Cincinnati Zoo & Botanical Garden, and Lori Marcum, Corteva Agriscience |
With the increasing threats of habitat loss, unsustainable use, and climate change to the world’s plant biodiversity, the need for ex situ conservation of plant genetic resources is also increasing. This is most efficiently accomplished with seed banks, but there are predicted to be tens of thousands of species for which conventional seed banking will be unworkable. These species are collectively known as exceptional plants and most will require cryopreservation methods in order to conserve them long-term. In vitro methods will be critical for many of these species, for providing tissues for cryopreservation and for propagating and recovering plants to restore species in the wild. Thus, there is an urgent need for developing in vitro protocols for a wide range of threatened plant taxa. This session provides a look at the work of three laboratories that are focused on this goal, representing a variety of species and illustrating the approaches and challenges of this work.
| 3:30 | Introduction (V. C. Pence and L. Marcum) | |
| 3:35 | P-27 | In Vitro Methods for the Conservation of Rare Plants of the Southeastern U.S. Emily Coffey, Atlanta Botanical Garden |
| 4:00 | P-28 | The Creation of In Vitro Collections to Conserve Forest Genetic Resources: The Magnolia and Avocado Cases Raquel Folgado, The Huntington Library, Art Museum, and Botanical Garden |
| 4:25 | P-29 | Ex-Situ Conservation of Critically Endangered Species at the Hawaiian Rare Plant Program Devon Gordon, The University of Hawai’i at Mãnoa |
| 4:50 | Discussion |
| 3:30 pm – 5:00 pm |
SINGLE CELL TECHNOLOGY AND APPLICATION IN BIOMEDICAL RESEARCH |
Pacific H&I |
| ANIMAL SYMPOSIUM | Conveners: Kristina Martinez-Guryn, Midwestern University and Rosa Ventrella, Midwestern University |
Single-cell omics technologies, such as genomics, epigenomics, transcriptomics, proteomics, and metabolomics have undergone rapid growth over the past decade. These technologies can analyze many cells at a high resolution making it a valuable tool in characterizing rare cell types and better understanding diverse cell states. Studying the cell-cell variation within cell populations can reveal unique mechanisms related to disease pathogenesis. The goal of this session is to describe the current state of research utilizing single-cell technologies and provide key examples of how these technologies are being used to identify how cellular heterogeneity contributes to human disease.
| 3:30 | Introduction (R. Ventrella) | |
| 3:35 | A-13 | Use of Single-cell Approaches to Investigate the Role of T Cell Memory in Health and Disease John Chang, University of California, San Diego |
| 4:00 | A-14 | Defining Epithelial Development at the Single Cell Level Scot Atwood, University of California, Irvine |
| 4:25 | A-15 | Utilizing Single Cell Analyses to Characterize Models of Human Cortical Organoids Aparna Bhaduri, University of California, Los Angeles |
| 4:50 | Discussion |
| 5:00 pm – 5:30 pm | SIVB Business Meeting (All Members Are Urged to Attend) Student Award Presentations |
Pacific F&G |
| 5:45 pm – 9:30 pm | Ships of the Seven Seas, Tuesday Evening at the Maritime Museum Admittance by Advanced Ticket Holders only |
Off Property |