Tuesday, June 13

For your viewing convenience, the 2017 In Vitro Biology Meeting Final Program has been broken down by day.

Daily Program
2017 In Vitro Biology Meeting
Saturday
June 10
Keynote Symposium Plant Contributed Papers
Sunday
June 11
Plenary Symposia Animal Posters
Monday
June 12
Animal Symposia and Workshops Education Silent Abstracts
Tuesday
June 13
Education Symposia and Workshops Plant Posters
Wednesday
June 14
Plant Symposia and Workshops Abstract Issue Index
Poster Sessions Animal Contributed Papers Late Submission Abstracts and Index

Daily Program-at-a-Glance

Time Event Location
7:00 am – 5:00 pm Registration Ballroom C Lobby
10:00 am – 3:30 pm Exhibits and Posters Ballroom C
Morning 7:00 am – 12:30 pm
7:00 am – 8:00 am In Vitro – Animal Editorial Board Meeting University AB
Student Affairs Breakfast Room 304
8:00 am – 10:00 am Scaffolds from Plants and Synthetic Materials for Human Tissue Engineering Room 306BC
 10:00 am – 10:30 am Coffee Break Ballroom C
Education Committee Meeting Governors Board Room
10:30 am Closing of City of Oaks Silent Auction Ballroom C
10:30 am – 12:30 pm Practical Media Improvement Using DOE: Case Study Comparisons of a Commercially Available MS Media Improvement Kit Room 306BC
Medicinal Plants Room 305
Microfluidics for Applied Biology            Room 306A
Afternoon 12:30 pm – 6:00 pm
12:30 Announcement of the City of Oaks Silent Auction Winners Ballroom C Foyer
12:30 pm – 1:30 pm 2018 Program Planning Committee Meeting University AB
1:30 pm – 2:30 pm Interactive Poster Sessions
Germplasm Preservation and Others
In Vitro Animal Cell Sciences Interactive Poster Session
Metabolic Engineering & Others
Ballroom C
2:30 pm – 3:30 pm Odd Poster Authors will be present Ballroom C
3:30 pm – 5:00 pm Posters to be removed from Exhibit Hall
Exosomes/Secretomes Room 306A
Start Up for Commercialization, Idea Development, and IP Capture Room 306BC
Secondary Metabolites Room 305
 5:00 pm – 5:30 pm SIVB Business Meeting
(All Members Are Urged to Attend)
Student Award Presentations
Room 306 BC
 5:45 pm – 9:00 pm A Night at the Museum
Admittance by Advance Ticket Holders Only
 Off Property

Tuesday, June 13

7:00 am – 5:00 pm Registration Ballroom C Lobby

SCAFFOLDS FROM PLANTS AND SYNTHETIC MATERIALS FOR HUMAN TISSUE ENGINEERING

Conveners:     Joshua Z. Gasiorowski, Midwestern University, and Pamela J. Weathers, Worcester Polytechnic Institute

8:00 am – 10:00 am Plenary Symposium Room 306BC

Advanced biomanufacturing is an important emerging discipline to generate new, biologically-relevant materials and devices. The process of biomanufacturing relies on exploiting cell-derived building blocks, materials and synthesis systems – most commonly from a single organism (i.e. plant, animal, or bacterial cell). Consequently, the scope of biomanufacturing has been limited by the physical and intellectual isolation of basic research in different organisms and systems to their respective biological “kingdoms.” Current limitations in biomanufacturing can be overcome through the development of novel engineering tools, and also by exploiting contributions across living kingdoms to use naturally occurring materials and/or bioinspired architecture. In this session new approaches will described for generating the architectural scaffolds for eventual use in tissue and organ engineering.

8:00

Introduction (J. Z. Gasiorowski and P. J. Weathers)

8:05  PS-6 Plantimals: Plant Tissues as Scaffolds for Human Tissue Engineering
Glenn Gaudette,
Worcester Polytechnic Institute
9:00  PS-7 TBD
Jennifer West, Duke University
9:55 Discussion
10:00 am – 10:30 pm Coffee Break Ballroom C

PRACTICAL MEDIA IMPROVEMENT USING DOE: CASE STUDY COMPARISONS OF A COMMERCIALLY AVAILABLE MS MEDIA IMPROVEMENT KIT

Conveners:     Randall P. Niedz, USDA-ARS U.S. Horticultural Research Laboratory, and Michael E. Kane, University of Florida

10:30 am – 12:30 pm Plant Symposium Room 306BC

Media improvement is critical for improving the growth and regeneration of plant tissues in vitro.  During the 2015 SIVB Meeting, a symposium was organized to discuss the benefits, challenges and the “nuts and bolts” of using a statistics-based approach, known as Design of Experiments (DOE) to improve plant tissue culture media. This approach provides researchers with a method to efficiently screen multiple media factors, simultaneously. However, systematic testing of the effects of the more than a dozen mineral salts included in most plant culture media, is sometimes perceived by laboratories as daunting and beyond their capabilities. To overcome this limitation PhytoTechnology Laboratories recently developed a DOE-based media kit, the Deconstructed MS Media Kit, to facilitate the use of DOE for media improvement. This session will review the DOE approach to media development, the DOE principles used by the MS Media Kit, and the kit’s utility. Research and commercial micropropagation laboratories will present case studies of the kit’s usage.  A discussion will follow the presentations.

10:30 Introduction (R. P. Niedz and M. E. Kane)
10:40 P-23 MS Media Kit and Design of Experiments (DOE) Overview
Randall P. Niedz, USDA-ARS U.S. Horticultural Research Laboratory
11:10 P-24 The PhytoTechnology Laboratories Deconstruction MS Media Kit
David Hart, PhytoTechnology Laboratories
11:30 -25 Case Study #2: Using the Deconstruction MS Media Kit to Evaluate Factors Influencing In Vitro Growth in Endangered Exceptional Species
Valerie Pence, Cincinnati Zoo and Botanical Garden
11:50 P-26 Screening Experiments for Mineral Nutrition Using De-constructed MS:  Observing Subsequent Effects on Rooting and Acclimatization
Jeffery Adelberg, Clemson University
12:10  Discussion


MEDICINAL PLANTS

Conveners:     Debora A.  Esposito, North Carolina State University, and Nirmal Joshee, Fort Valley State University

10:30 am – 12:30 pm Plant Symposium Room 305

From Alaskan berries to chicory roots, there are hundreds of remarkably common herbs, flowers, berries and plants that serve all kinds of important medicinal and health purposes that might surprise you. Medicinal Plants 2017 session will showcase recent discoveries and developments in Natural Products drugs discovery and development.

10:30 Session Overview (D. A. Esposito and N. Joshee)
10:35 P-27 Technological and Health Relevant Attributes of Spray Dried Blueberry Polyphenol-protein Aggregates
Roberta Targino Pinto Correia, Universidade Federal do Rio Grande do Norte
11:00 P-28 Dietary Long-chain PUFAs Enrich Porcine Alveolar Macrophages and Modulate Inflammatory Response
Kathleen Walter, North Carolina State University
11:25 P-29 Berry Extracts to Protect Skin from Inflammation
Sierra Bonney, North Carolina State University
11:50 P-30 Bitter Receptors Control Glucose Absorption in the Gut by Modifying the GPCR Signaling Cascade
Kimberly M. Palatini-Jackson, North Carolina State University
12:15 Discussion

MICROFLUIDICS FOR APPLIED BIOLOGY

Conveners:     Jessica Monserrate, Bayer CropScience, Miguel Acosta, Arbiom, and Sara Lenzen, Bayer CropScience

10:30 am – 12:30 pm Animal Symposium Room 306A

Microfluidics is a popular field in the life sciences and biotechnology because it can be customized for a wide range of application including: drug delivery, point of care devices and diagnostics as well affording researchers the ability to analyze single cell responses to soluble cues.  This is but a small sliver of possibilities and the diversity of applications is only growing. In this session we touch on some of the microfluidic tools being developed for applied biology.

10:30 Introduction (J. Monserrate and M. Acosta)
10:35 A-11 Development of a Tumor-on-a-Chip for Hypoxia Studies
Glenn Walker, North Carolina State University
11:00 A-12 Liquid Metals for Microfluidics
Michael Dickey, North Carolina State University
11:35 A-13 Programming Bacteria in Time and Space
Lingchong You, Duke University
12:10 Discussion

IN VITRO ANIMAL CELL SCIENCES INTERACTIVE POSTER SESSION

Moderator:             Durga Attili, University of Michigan Medical School

1:30 pm – 2:30 pm Animal Interactive Poster Session Ballroom C
A-2000 Protective Effects of Oat Bioactives on Biomarkers of Gastrointestinal Cell Injury
Mikayla M. Bowen, University of North Carolina Chapel Hill, Ashley Buige, Weston Bussler, and Slavko Komarnytsky
A-2001 Absorption and Distribution of the Antimalarial Drug Artemisinin Delivered Orally as Dried Leaves of Artemisia annua
Matthew Desrosiers, Worcester Polytechnic Institute, and P. J. Weathers
A-2003 Identifying the Role of Phosphorylation on Interdomain Communication in Hsp70
Laura Knighton, University of North Carolina at Charlotte, and Andrew W. Truman 
A-2004 A Histological Comparison of Ear Skin Regeneration in Acomys and Mus.
A. Sandoval, University of Florida, J. Brant, and M. Maden
 A-2007 Opportunities for Translating Large-scale Cell Culture Technologies to the Production of Sustainable Clean Meat
Christie Lagally, The Good Food Institute, and Liz Specht

GERMPLASM PRESERVATION AND OTHERS

Moderator:      Hong Luo, Clemson University

1:30 pm – 2:30 pm Plant Interactive Poster Session Ballroom C
P-2003 A Lab-to-Land Approach for Conservation of Shirui Lily (Lilium macklineae) – An Endangered Heritage Flower of Manipur, India.
M. R. Sahoo, ICAR Research Complex for North Eastern Hill Region, M. P. Devi, M. Dasgupta, N. Prakash, and S. V. Ngachan
P-2004 Plant Production Through Somatic Embryogenesis of Theobroma cacao L. Leaf Cultures
Esther E. Uchendu,
University of Ibadan, Omolola O. Oso, and Victor O. Adetimirin
P-2005 Improving Resistance to Potato Common Scab by the Production of Somaclones Habituated to Thaxtomin A
Safa Labidi, University of Sherbrooke, and Nathalie Beaudoin

METABOLIC ENGINEERING AND OTHERS

Moderator:      Margaret M. Young, Elizabeth City State University

1:30 pm – 2:30 pm Plant Interactive Poster Session Ballroom C
P-2010 Red and White PAP1-controlled Arabidopsis Cells Are Dependent Upon TT8
Yue Zhu, North Carolina State University, and De-Yu Xie
P-2011 Suspension Culture of Programmed Red vs. Wild-type Cells of Tobacco
Seyit Yuzuak, North Carolina State University, and De-Yu Xie
P-2012 Generation of Chemically Induced Mutations Using Embryogenic Coffee Cell Suspensions and In Vitro Selection for Salt Tolerance
Andrés M Gatica-Arias, Universidad de Costa Rica, Alejandro Bolivar-González, and Marta F Valdez-Melara

Tuesday, June 13
Odd Poster Authors will be present
2:30 pm – 3:30 pm

EXOSOMES/SECRETOMES

Conveners:     Michael J. Fay, Midwestern University, and Kolla Kristjansdottir, Midwestern University

3:30 pm – 5:00 pm Animal Symposium Room 306A

This session will focus on two methods of intercellular communication, expression of intracellular proteins ectopically on the cell surface and secretion of exosomes that contain proteins, lipids, and RNAs. Hsp90 is an intracellular protein that has been extensively studied as a chemotherapeutic drug target. Recently it has been shown that a unique form of Hsp90 is ectopically expressed on the cell surface of cancer cells and may mediate migration from the primary tumor site. In this session, a tethered Hsp90 inhibitor will be introduced that recognizes only the extracellular form of Hsp90 and results in specific labeling of cancerous cells. Another method of intercellular communication involves secretion of exosomes that can contain proteins, lipids, and RNAs. The involvement of exosomes in intercellular communication has become a major focus of research, and investigators are trying to determine the role of exosomes in physiological and pathophysiological processes. In this session we will learn about the potential for exosomes to be used to transport chemotherapeutic drugs to cancer cells. We will also learn about the role of exosomes in ocular health and disease.

3:30 Introduction (M. J. Fay and K. Kristjansdottir)
3:35  A-15 Fluor-Tethered Inhibitors of Hsp90 Reveal Secretion and Reinternalization of Hsp90 Is Associated with Metastatic Progression
Timothy Haystead, Duke University School of Medicine
4:00  A-16 Using Exosomes for Drug Delivery
Elena Batrakova, University of North Carolina at Chapel Hill
4:25  A-17 The Potential of Ocular Exosomal Biomarkers as Therapeutic Targets, and as Diagnostic and Prognostic Indicators
Mikael Klingeborn, Duke University Medical Center
4:50 Discussion

START UP FOR COMMERCIALIZATION, IDEA DEVELOPMENT, AND IP CAPTURE

Conveners:     Allan R. Wenck, Bayer CropScience, and Hoang Nguyen, University of California, Davis

3:30 pm – 5:00 pm Plant Symposium Room 306BC

The contributions of biological scientists to society have surpassed the boundary of academia. There are many successful biotechnology companies created or operated by biological scientists. The advancement of the internet-shared information, new biological technologies and especially start-up incubators has effectively nurtured many research ideas into biological businesses. Various case studies indicated that the process of turning ideas to fruitful companies may or may not be generalized by a linear pathway. Typically, a biological startup begins with (1) preliminary research and idea development, or opportunity recognition, (2) intellectual property rights protection, (3) fund raising by obtaining venture capital, (4) team building toward technology and product development, (5) product marketing and profit making. At the Research Triangle Park (RTP) in North Carolina, a startup hub has been created to support agricultural scientists and businesses. Biological scientists and inventors, from various startup backgrounds at the RTP, will share their experience on the process of establishing and running a biotechnology start-up as described above. This session will provide a comprehensive picture that would motivate young scientists to view biological entrepreneurship as an essential future skill.

3:30 Introduction (A. R. Wenck and H. Nguyen)
3:35 P-31 Underground Signaling Networks
Philip Benfey, Duke University
3:55 P-32 How to Build a Business While You are Deciding on the Perfect Product or Technology to Commercialize?
David Reed, Mimetics
4:15 P-33 Start Up for Commercialization, Idea Development, and IP Capture: How to Obtain Venture Capital
Jeffrey L. Rosichan, AgTech Accelerator
4:35 P-34 Intellectual Property Protection for Early Stage Companies
Alice M. Bonnen, Myers Bigel, P. A.
4:55 Discussion

SECONDARY METABOLITES

Moderator:      Jeffrey W. Adelberg, Clemson University

3:30 pm – 4:10 pm Plant Contributed Paper Session Room 305
3:30 P-1015 A Novel Approach to Cell Selection from Taxus Plant Cell Culture Via an Engineered Mammalian Caspase.
Michelle Mckee, Worchester Polytechnic Institute, Maureen Hill, Jeanne Hardy, and Susan Roberts
3:50 P-1016 In Vitro Anther Cultures of Camellia assamica ssp. assamica (Masters) for Haploid Plant Production and Possibilities of Accumulation of Catechins, Caffeine and Theophylline in Them
Rakhi Chaturvedi, Indian Institute of Technology Guwahati, Vijay Kumar Mishra, and Ruchira Bajpai

 

5:00 pm – 5:30 pm SIVB Business Meeting
(All Members Are Urged to Attend)
Student Award Presentations
Room 306BC
5:45 pm – 9:00 pm A Night at the Museum
Admittance by Advance Ticket Holders Only
Off Property