For your viewing convenience, the 2017 In Vitro Biology Meeting Final Program has been broken down by day.
Daily Program-at-a-Glance
| Time | Event | Location | ||||||
|---|---|---|---|---|---|---|---|---|
| 7:00 am – 5:30 pm | Registration | Ballroom C Lobby | ||||||
| 10:00 am – 2:00 pm 5:30 pm – 7:30 pm |
Exhibits and Posters | Ballroom C | ||||||
| Morning | 7:00 am – 12:30 pm | |||||||
| 7:00 am – 9:00 am | In Vitro Animal Cell Sciences Program Committee Meeting | Room 306A | ||||||
| Plant Biotechnology Program Committee Meeting | Room 304 | |||||||
| 8:00 am – 10:00 am | Innovative Advances in Flow Cytometry and Cell Sorting for Plant & Mammalian Cells | Room 306BC | ||||||
| 10:00 am – 10:30 am | Coffee Break | Ballroom C | ||||||
| Nominating Committee Meeting | University AB | |||||||
| Public Policy Committee Meeting | Governors Board Room | |||||||
| 10:30 am – 12:30 pm | Cannabis: Past, Present and Future | Room 306BC | ||||||
| Role of Secondary Metabolites/Antioxidants In Vitro | Room 305 | |||||||
| From Single Cell Analysis to Multiplexed Screening Assays of Cell | Room 306A | |||||||
| 11:00 am – 12:00 pm | SIVB/IAPB/Springer Business Meeting | University AB | ||||||
| Afternoon | 12:30 pm – 6:00 pm | |||||||
| 12:30 pm – 1:30 pm | Membership Committee Meeting | Governors Board Room | ||||||
| In Vitro – Plant Editorial Board Meeting | University AB | |||||||
| Exhibitors/SIVB Refreshment Break | Ballroom C | |||||||
| 1:30 pm – 3:00 pm | Advances in Woody and Ornamental Plant Tissue Culture | Room 305 | ||||||
| Biosafety Best Practices for GMOs and Regulatory Challenges with Genome Editing | Room 306BC | |||||||
| Expert’s Corner | Room 306A | |||||||
| 3:00 pm – 5:00 pm | Cell Culture Systems for Agricultural and Environmental Research | Room 306A | ||||||
| Plant Biotechnology Student Oral Presentation Competition | Room 305 | |||||||
| Genetic Engineering I and Plant Biotechnology Post Doctoral Oral Presentation Competition | Room 304 | |||||||
| 5:00 pm – 6:00 pm | 2017 In Vitro Biology Meeting Opening Ceremony
|
Room 306BC | ||||||
| Evening | 6:00 pm – 9:30 pm | |||||||
| 6:00 pm – 7:30 pm | Opening Ceremony Reception | Ballroom C | ||||||
| 6:30 – 7:30 pm | Poster Session Odd Poster Authors will be present |
Ballroom C | ||||||
| 7:30 pm – 9:30 pm | Student Symposium: Persuasive Presentations: Tips and Techniques for Public Speaking | Room 304 |
Sunday, June 11
| 7:00 am – 5:30 pm | Registration | Ballroom C Lobby |
INNOVATIVE ADVANCES IN FLOW CYTOMETRY AND CELL SORTING FOR PLANT & MAMMALIAN CELLS
Conveners: Michael K. Dame, University of Michigan Medical School, and Tobias M. Cicak, Dow AgroSciences
| 8:00 am – 10:00 am | Plenary Symposium | Room 306BC |
Flow cytometry is an established research and clinical workhorse for interrogating single cell biology for humans and other animal systems, and has emerged as an invaluable tool for high throughput multiparametric analysis of plant single cells. Fluorescent labeling and microscopic analysis of plant cell markers has been critical for the development of transformation methods and understanding plant cell biology, but these methods can be time consuming and provide only a limited representation of the total cell population. Flow cytometry can now provide quantitative statistical analysis of cellular activity, the determination of ploidy level, and a robust and rapid platform for testing genome editing techniques. This session will reveal the effects of methyl jasmonate elicitation on cell cycle of Taxus cultures through flow cytometric examination of 5-ethynyl-20-deoxyuridine (EdU) pulse labeled cells. Finally this session will introduce a novel flow cytometry technology which revolutionizes the analysis of fragile cells, such as animal and plant stem cells, and patient-derived cells for disease therapy.
| 8:00 |
Introduction (M. K. Dame and T. M. Cicak) |
|
| 8:05 | PS-1 |
Quantifying Heterogeneity in Plant Cell Culture Using Flow Cytometric Methods |
| 9:00 | PS-2 | Sterile Sorting of Human Cells for In Vitro Manipulation and Expansion Prior to Clinical Transplantation Kevin Beck, Miltenyi Biotec |
| 9:55 | Discussion |
| 10:00 am – 10:30 am | Coffee Break | Ballroom C |
CANNABIS: PAST, PRESENT AND FUTURE
Conveners: Hemant Lata, University of Mississippi, and Theodore M. Klein, Pioneer Crop Genetics
| 10:30 am – 12:30 pm | Plant Symposium | Room 306BC |
In recent years, cannabis has gained considerable attention among researchers and the general public, in large part because its medicinal properties are becoming more widely recognized. And although there is the potential for abuse of cannabis and it is still not considered legal by the federal government, cannabis has been deemed legal for medicinal or recreational use in many states.Cannabis produces a wide range of valuable secondary metabolites that include the cannabinoids, a unique class of terpenophenolic compounds found in its glandular trichromes. So far, out of 565 seconday compounds identified in Cannabis, 120 are cannabinoids. The plant’s behavioral and psychotropic effects are attributed to Δ9-Tetrahydrocannabinol (Δ9-THC) commonly known as THC. Other major cannabinoids include cannabigerol (CBG), cannabichromene (CBC), cannabidiol (CBD), tetrahydrocannabivarin (THCV) and cannabinol (CBN). The recent discoveries of medicinal properties of cannabis and the cannabinoids and their potential applications in the treatment of a number of serious illnesses such as glaucoma, depression, neuralgia, multiple sclerosis, Alzheimers, alleviation of symptoms of HIV/AIDS, cancer and childhood epilepsy, have given momentum to the quest for understanding the botany, chemistry and biology of this plant. In this session, the evolution of cannabis, its chemistry, potency as a psychotropic drug, and the current status and future development as a phytopharmaceutical will be discussed.
| 10:30 |
Introduction (H. Lata and T. M. Klein) |
|
| 10:35 | P-1 | Evolution and Germplasm Conservation of Economic Groups of Cannabis Ernest Small, Agriculture and Agri-Food Canada |
| 11:10 | P-2 | Cannabis sativa L: Botany, Chemistry and Drug Development Mahmoud A. ElSohly, The University of Mississippi |
| 11:45 | P-3 | Cannabinoid Dosage Formulations – From the Field, Pharmacy, Dispensary and Street Brian F. Thomas, RTI International |
| 12:30 | Discussion |
ROLE OF SECONDARY METABOLITES/ANTIOXIDANTS IN VITRO
Convener: Maria M. Jenderek, ARS – USDA
| 10:30 am – 12:30 pm | Plant Symposium | Room 305 |
In literature, secondary metabolites are described as natural products, waste, phytopharmaceuticals, bioactive constituents or by-products of the primary metabolism. They occur in many plant genera and microorganisms in vivo and in vitro, and have complex chemical structures specific to the plants where they are found. Some of them are used in production of healthcare substances, food additives, flavor and color enhancers, nutraceuticals, fragrances and agrochemicals. Previously thought of as not being critical in plant functioning, they have a significant role in plant survival in their environment and in regulation of primary metabolic pathways. A large number of unique secondary metabolites have been identified; however, the function of many and their metabolic pathways are yet to be characterized. The session will discuss research on describing biosynthesis pathways of auxins and anthocyanins and the role of specific antioxidants in in-vitro plant cultures.
| 10:30 | Introduction (M. M. Jenderek) | |
| 10:35 | P-4 | The Role of Local Auxin Biosynthesis in Plant Development Anna Stepanova, North Carolina State University |
| 11:00 | P-5 | Regulation of Anthocyanin Biosynthesis in the WD40-bHLH-MYB Complex-Programmed Arabidopsis Cells Deyu Xie, North Carolina State University |
| 11:25 | P-6 | A New Balancing Act: Melatonin and Serotonin as Mediators of Plant Morphogenesis Lauren AE Erland, University of Guelph |
| 11:50 | P-7 | Role of Antioxidants in In Vitro Plant Culture Systems Praveen Saxena, University of Guelph |
| 12:15 | Discussion |
FROM SINGLE CELL ANALYSIS TO MULTIPLEXED SCREENING ASSAYS OF CELL CULTURES
Conveners: Justin Colacino, University of Michigan School of Public Health, and Addy Alt-Holland, Tufts University
| 10:30 am – 12:30 pm | Animal Symposium | Room 306A |
The number of assays designed to collect data from cell cultures and individual cells are rapidly expanding and are poised to revolutionize our understanding of cellular biology. However, there are also unanticipated technical and analytical challenges in the implementation of single cell analytics into typical in vitro biology workflows. Here we will highlight new applications in single cell analytics and heterogeneous cell cultures, ranging from cell health and viability to the generation high throughput ‘omics data while focusing on the generation of highly reproducible data. The first presentation will outline the utility of single cell assays, including flow cytometry and single cell transcriptomics, to identify and characterize rare cell populations, such as stem cells or circulating tumor cells. The second presentation will focus on ways to improve the reproducibility of cell-based assays and approaches to multiplex distinct cell health measurements to simultaneously analyze multiple parameters in a given sample of cells.
| 10:30 | Introduction (J. Colacino and A. Alt-Holland) | |
| 10:35 | A-1 | Single Cell Transcriptomics: Unraveling Heterogeneity in Environmental Health and Cancer Justin A. Colacino, University of Michigan School of Public Health |
| 11:10 | A-2 | Treating Cells as Reagents to Achieve Assay Reproducibility Terry Riss, Promega Corporation |
| 11:45 | A-3 | In Vitro Methods for Measuring Cell Health in Real-Time Terry Riss, Promega Corporation |
| 12:20 | Discussion |
ADVANCES IN WOODY AND ORNAMENTAL PLANT TISSUE CULTURE
Convener: Paula M. Pijut, USDA Forest Service
| 1:30 pm – 3:00 pm | Plant Symposium | Room 305 |
Research on in vitro ploidy manipulation of nursery and ornamental woody species, as well as ornamental and bioenergy grasses, and the use of molecular markers to assess hybridity will be discussed. In addition, a high-throughput transformation system in Populus that enables the testing of large numbers of candidate genes will be described. This system has been used to produce transgenic lines representing over 600 gene constructs in a joint program focused on understanding and eliminating biomass recalcitrance in order to improve biofuel yields.
| 1:30 | Introduction (P. M. Pijut) | |
| 1:35 | P-8 | In Vitro Ploidy Manipulation for Ornamental and Bioenergy Crop Improvement Darren Touchell, North Carolina State University |
| 2:10 | P-9 | High Throughput Transformation Systems J. Eric Gulledge, ArborGen, Inc. |
| 2:55 | Discussion |
BIOSAFETY BEST PRACTICES FOR GMOS AND REGULATORY CHALLENGES WITH GENOME EDITING
Conveners: Annie Saltarikos, Monsanto Company, and Marceline Egnin, Tuskegee University
| 1:30 pm – 3:00 pm | Plant Symposium | Room 306BC |
As the world’s population continues to rise and the amount of arable farmland remains static, there is a vested interest to continue to develop high-yielding crops for food and feed, while using less inputs. The last several decades have seen a rapid adoption and deployment of biotechnology practices across the major crop species to deliver genes or favorable traits (both foreign and plant-derived) to increase yield, quality, and provide resistance to both biotic and abiotic stresses. Alongside these advances, best practices in biosafety and regulatory pathways guiding the risk assessment of GM crops to their commercialization have been gained since the 90’s. However, public perception of these technologies’ safety has been mixed, and the regulatory trigger pathway for these products is complex but limited in its current practices. Most recently, the advent and relative ease and speed of use of genome engineering tools such as Clustered Regularly Interspersed Short Palindromic Repeats (CRISPR)-Cas9 has dramatically cut down the previous bottlenecks of having to provide protein engineering to target specific DNA sequences within the genome; thus lowering the barrier to rapid technical advancements in this field of precision genome engineering for crops. However, just as quickly as the number of technical advancements in this emerging field arise, so do the questions and challenges of how these precision engineered plant products will be further regulated and perceived by the public in terms of safety and acceptance. The advantages of genome editing are multi fold, as it can provide non-transgenic methods of creating desirable traits through direct manipulation of native alleles, as well as can accelerate deployment of genome edited products across crops through potentially shortening US regulation oversight triggers that have limited deployment of traditional GM crops in distinct regulation boundaries. This sessions’ speakers from industry, Institutions, academia, and government will share both the current regulatory and biosafety landscape for genome edited products as well as discuss the challenges and best regulatory practices.
| 1:30 | Introduction (A. Saltarikos and M. Egnin) | |
| 1:35 | P-10 | Biotech and Genome Editing Regulations Alan McHughen, University of California – Riverside |
| 2:00 | P-11 | Engineering the DNA of Our Governance Systems: Biosafety Best Practices and Issues for GMOs Todd Kuiken, North Carolina State University |
| 2:25 | P-12 | Regulatory Considerations for Genome Editing in Crops Miguel Vega-Sanchez, Monsanto Company |
| 2:50 | Discussion |
EXPERT’S CORNER
Moderator: Addy Alt-Holland, Tufts University
| 1:30 pm – 3:00 pm | Animal Contributed Paper Session | Room 306A |
| 1:30 | Introduction (A. Alt-Holland) | |
| 1:35 | A-1003 | In Bioassays, Blind Faith Is a Fool’s Errand-Run the Controls Every Time John W. Harbell, JHarbell Consulting LLC |
| 1:55 | A-1004 | An Adventure in Serum-free Cell Culture Denry Sato, Manzanar Project Foundation |
| 2:15 | A-1005 | Gap Junctional Intercellular Communication, the Archtypal Cell Phone of Tissues, Is an Excellent Biomarker for Assessing Environmental Stressors and Chemopreventive Compounds Brad L. Upham, Michigan State University, Iva Sovadinova, and Pavel Babica |
| 2:35 | A-1006 | Intrinsic Electrical Signal Characteristics of Rat Embryonic Cortex Tissue Nurdan Ozkucur, Tufts University |
| 2:55 | Discussion |
CELL CULTURE SYSTEMS FOR AGRICULTURAL AND ENVIRONMENTAL RESEARCH
Conveners: Vivian R. Dayeh, University of Waterloo, and Cynthia L. Goodman, USDA-ARS-BCIRL
| 3:00 pm – 5:00 pm | Animal Symposium | Room 306A |
In vitro approaches play an important role in dissecting and understanding complex relationships within agricultural and environmental systems. Continuously replicating cell lines as well as primary cell cultures from an array of species offer meaningful insights into physiological and toxicological impacts of agricultural, industrial, and other human-based inputs into the environment. Likewise, cell cultures are valuable tools for both screening and mechanism of action studies in AgDiscovery programs aimed at developing pest-specific products. Our session will showcase cell-based assays that are being used to investigate xenobiotic impacts on human lung function, amphibian immunity, insect digestive processes, and invertebrate cell viability.
| 3:00 | Introduction – Environmental Presentations (V. R. Dayeh) | |
| 3:05 | A-4 | Modeling Human Lung Fibroblast Responses in Asthma and Airway Disease Jennifer Leigh Ingram, Duke University Medical School |
| 3:30 | A-5 | Expanding the Xenopus laevis Invitrome: Establishing and Characterizing Cell Lines For Use in Examining Host-Pathogen-Environment Interactions Barb Katzenback, University of Waterloo |
| 3:55 | Introduction – Agricultural Presentations (C. L. Goodman) | |
| 4:00 | A-6 | Insights on the Mode of Action of Insecticidal Proteins from Bacillus thuringiensis Toxins from Cell Cultures Juan Luis Jurat-Fuentes, University of Tennessee |
| 4:25 | A-7 | Establishing Cell Lines from Pest Insects: Studies of Prostaglandin Actions David Stanley, USDA, ARS, BCIRL |
| 4:50 | Roundtable discussion including all speakers |
PLANT BIOTECHNOLOGY STUDENT ORAL PRESENTATION COMPETITION
Moderator: Jeffrey Beringer, Dow AgroSciences
Panel of Plant Biotechnology Experts Evaluating the Contestants: Barbara M. Reed, USDA-ARS Retired; Terry Hu, DuPont Pioneer, and Sergei F. Krasnyanski, North Carolina State University
| 3:00 pm – 5:00 pm | Plant Contributed Paper Competition | Room 305 |
To support the Society’s vision to encourage education and scientific informational exchange and recognize outstanding students, the Plant Biotechnology Section is pleased to announce the 2017 Student Oral Presentation Competition. Student competition finalists were selected based on the quality of the abstracts. A panel of judges will evaluate the presentations at the meeting. Criteria for the evaluation include experimental design, data analysis, proper interpretation of the results, originality of the study, technical difficulty, appearance and ability of the student candidate to present it. Winners will be presented with a certificate and a cash award at the meeting.
| 3:00 | P-6 | A New Balancing Act: Melatonin and Serotonin as Mediators of Plant Morphogeneis Lauren A. E. Erland, University of Guelph, and Praveen K. Saxena |
| 3:20 | P-1000 | Effect of Dessication Stress and Subsequent Recovery on the in Vitro Growth of the Epiphytic Orchid Dendrophylax lindenii Jameson Coopman, University of Florida, and Michael Kane |
| 3:40 | P-1001 | Soybean (Glycine max) Promoter Characterization Through CRISPRi Andika Gunadi, The Ohio State University, and John J. Finer |
| 4:00 | P-1002 | A CRISPR Toolkit for Plants Jacob W. Hoyle, University of Georgia, Peter LaFayette, and Wayne Parrott |
| 4:20 | P-1003 | This abstract has been withdrawn |
| 4:40 | P-1004 | Enhancement of Halotolerance in Fremyella diplosiphon by Electroporation-mediated Overexpression of Malate Dehydrogenase Gene Behnam Tabatabai, Morgan State University, AnithaChristy Arumanayagam, and Viji Sitther |
GENETIC ENGINEERING I AND PLANT BIOTECHNOLOGY POST DOCTORAL ORAL PRESENTATION COMPETITION
Moderator: Piero Barone, Dow AgroSciences
| 3:00 pm – 5:00 pm | Plant Contributed Paper Session and Plant Contributed Paper Competition |
Room 304 |
| 3:00 | P-1005 | Global Scale Investigation of Soybean MicroRNAs Responsive to Soybean Cyst Nematodes Infection Bin Tian, Kansas State University, Shichen Wang, Charles D. Johnson, Guiliang Tang, and Harold N. Trick |
| 3:20 | P-1006 | It’s About Time; Rapid and Simple Maize Transformation George J. Hoerster, DuPont/Pioneer, Keith Lowe, Keith Lowe, Mauricio La Rota, Craig Hastings, Ning Wang, Emily Wu, Ajith Anand, Maren Arling, Brian Lenderts, Mark Chamberlin, Todd Jones, and William Gordon-Kamm |
| 3:40 | P-1007 | Under the Radar: Enhanced Legume Transformation by Altering the Host Receptor – Agrobacterium Effector Interactions Timothy M Chappell, University of Georgia, Brian H Kvitko, and Wayne Parrott |
| 4:00 | P-1008 | Employing Linear Minimal DNA Expression Cassettes for Sugarcane Genetic Transformation Using Biolistics Approach Muhammad Sohail Akram, Government College University, and Javed Iqbal |
Panel of Plant Biotechnology Experts Evaluating the Contestants: Prakash Kumar, National University of Singapore, Dennis Gray, University of Florida, and Geny Anthony, Dow AgroSciences
To support the Society’s vision to encourage education and scientific informational exchange and recognize outstanding post docs, the Plant Biotechnology Section is pleased to announce the 2017 Post-Doctoral Oral Presentation Competition. Postdoctoral competition finalists were selected based on the quality of the abstracts. A panel of judges will evaluate the presentations at the meeting. Criteria for the evaluation include experimental design, data analysis, proper interpretation of the results, originality of the study, technical difficulty, appearance and ability of the post-doctoral candidate to present it. Winners will be presented with a certificate and a cash award at the meeting.
| 4:20 | P-1009 | Improving Agrobacterium-mediated Transformation of Cowpea (Vigna unguiculata L.) Zhifen Zhang, University of Georgia, Kathleen Monfero Marasigan, Yinping Guo, and Peggy Ozias-Akins |
| 4:40 | P-1010 | Enhanced Production of Prenylated Stilbenoids and Elucidation of Their Biosynthetic Pathway in Hairy Root Cultures of Peanut Tianhong Yang, Arkansas State University, Lingling Fang, Keithanne Mockaitis, and Fabricio Medina-Bolivar |
2017 IN VITRO BIOLOGY MEETING OPENING CEREMONY
Program Chair: Addy Alt-Holland, Tufts University
| 5:00 pm – 6:00 pm | Opening Ceremony | Room 306BC |
Conveners: Addy Alt-Holland, Tufts University, and Dwight T. Tomes
| 5:00 | Welcome and Opening Remarks: Dwight T. Tomes, President, Society for In Vitro Biology |
|||||||
| 5:05 | 2017 Fellow, Distinguished Scientist, and Young Scientist Award Recipients (Awards to be presented at Section Meetings)
|
|||||||
| 5:15 | 2017 Distinguished Service Award Presentations (Awards to be presented by Dwight Tomes): Vadim Beilinson, AgBiome |
|||||||
| 5:30 | 2017 Lifetime Achievement Award Presentations (Introduction by Sandra Schneider; Acknowledgement by Dr. Sato to follow) J. Denry Sato, Manzanar Project Foundation |
|||||||
| 5:45 | (Introduction by Baochun Li, Acknowledgement by Dr. Phillips to follow) Gregory C. Phillips, Arkansas State University |
|||||||
| 6:00 | Adjourn |
| 6:00 pm – 7:30 pm | 2017 IN VITRO BIOLOGY MEETING OPENING CEREMONY RECEPTION | Ballroom C |
| 6:00 pm – 7:30 pm | Exhibits and Posters | Ballroom C |
Sunday, June 11
Odd Poster Authors will be present
6:30 pm – 7:30 pm
STUDENT SYMPOSIUM: PERSUASIVE PRESENTATIONS:
TIPS AND TECHNIQUES FOR PUBLIC SPEAKING
Conveners: Elena Arthur, North Carolina Central University, and Whitney Harchenko, Montana State University
| 7:30 pm – 9:30 pm | Education Symposium | Room 304 |
Public speaking is frequently ranked as the number one fear for adults — coming in higher than fear of death! Yet speaking publicly is increasingly becoming a requirement for many jobs. Luckily, effective public speaking is not magic. There are specific skills that enhance presentation delivery, strategies for program development, and tips for converting speaker anxiety into energy. This interactive workshop provides participants with practical tools for public speaking in any setting.
| 7:30 | Introduction (E. Arthur and W. Harchenko) | |
| 7:35 | E-1 | Persuasive Presentations: Tips and Techniques for Public Speaking Cally Ritter, AllOne Health |
| 8:35 | Discussion |