Sunday, June 11

For your viewing convenience, the 2017 In Vitro Biology Meeting Final Program has been broken down by day.

Daily Program
2017 In Vitro Biology Meeting
June 10
Keynote Symposium Plant Contributed Papers
June 11
Plenary Symposia Animal Posters
June 12
Animal Symposia and Workshops Education Silent Abstracts
June 13
Education Symposia and Workshops Plant Posters
June 14
Plant Symposia and Workshops Abstract Issue Index
Poster Sessions Animal Contributed Papers Late Submission Abstracts and Index

Daily Program-at-a-Glance

Time Event Location
7:00 am – 5:30 pm Registration Ballroom C Lobby
10:00 am – 2:00 pm
5:30 pm – 7:30 pm
Exhibits and Posters Ballroom C
Morning 7:00 am – 12:30 pm
7:00 am – 9:00 am In Vitro Animal Cell Sciences Program Committee Meeting Room 306A
Plant Biotechnology Program Committee Meeting Room 304
8:00 am – 10:00 am Innovative Advances in Flow Cytometry and Cell Sorting for Plant & Mammalian Cells Room 306BC
10:00 am – 10:30 am Coffee Break Ballroom C
Nominating Committee Meeting University AB
Public Policy Committee Meeting Governors Board Room
10:30 am – 12:30 pm Cannabis: Past, Present and Future Room 306BC
Role of Secondary Metabolites/Antioxidants In Vitro Room 305
From Single Cell Analysis to Multiplexed Screening Assays of Cell Room 306A
11:00 am – 12:00 pm SIVB/IAPB/Springer Business Meeting University AB
Afternoon 12:30 pm – 6:00 pm
12:30 pm – 1:30 pm Membership Committee Meeting Governors Board Room
In Vitro – Plant Editorial Board Meeting University AB
Exhibitors/SIVB Refreshment Break Ballroom C
1:30 pm – 3:00 pm Advances in Woody and Ornamental Plant Tissue Culture Room 305
Biosafety Best Practices for GMOs and Regulatory Challenges with Genome Editing Room 306BC
Expert’s Corner Room 306A
3:00 pm – 5:00 pm Cell Culture Systems for Agricultural and Environmental Research Room 306A
Plant Biotechnology Student Oral Presentation Competition Room 305
Genetic Engineering I and Plant Biotechnology Post Doctoral Oral Presentation Competition Room 304
5:00 pm – 6:00 pm 2017 In Vitro Biology Meeting Opening Ceremony

5:00 Welcome and Opening Remarks:
5:05 2017 Fellow, Distinguished Scientist, and Young Scientist Award Recipients
5:30 2017 Lifetime Achievement Award Presentations:
Room 306BC
Evening 6:00 pm – 9:30 pm
6:00 pm – 7:30 pm Opening Ceremony Reception Ballroom C
6:30 – 7:30 pm Poster Session
Odd Poster Authors will be present
Ballroom C
7:30 pm – 9:30 pm Student Symposium: Persuasive Presentations: Tips and Techniques for Public Speaking Room 304

Sunday, June 11

7:00 am – 5:30 pm Registration Ballroom C Lobby


Conveners:     Michael K. Dame, University of Michigan Medical School, and Tobias M. Cicak, Dow AgroSciences

8:00 am – 10:00 am Plenary Symposium Room 306BC

Flow cytometry is an established research and clinical workhorse for interrogating single cell biology for humans and other animal systems, and has emerged as an invaluable tool for high throughput multiparametric analysis of plant single cells. Fluorescent labeling and microscopic analysis of plant cell markers has been critical for the development of transformation methods and understanding plant cell biology, but these methods can be time consuming and provide only a limited representation of the total cell population. Flow cytometry can now provide quantitative statistical analysis of cellular activity, the determination of ploidy level, and a robust and rapid platform for testing genome editing techniques. This session will reveal the effects of methyl jasmonate elicitation on cell cycle of Taxus cultures through flow cytometric examination of 5-ethynyl-20-deoxyuridine (EdU) pulse labeled cells. Finally this session will introduce a novel flow cytometry technology which revolutionizes the analysis of fragile cells, such as animal and plant stem cells, and patient-derived cells for disease therapy.


Introduction (M. K. Dame and T. M. Cicak)

8:05  PS-1

Quantifying Heterogeneity in Plant Cell Culture Using Flow Cytometric Methods
Susan Roberts, Worcester Polytechnic Institute

9:00  PS-2 Sterile Sorting of Human Cells for In Vitro Manipulation and Expansion Prior to Clinical Transplantation
Kevin Beck, Miltenyi Biotec
9:55 Discussion
10:00 am – 10:30 am Coffee Break Ballroom C


Conveners:     Hemant Lata, University of Mississippi, and Theodore M. Klein, Pioneer Crop Genetics

10:30 am – 12:30 pm Plant Symposium Room 306BC

In recent years, cannabis has gained considerable attention among researchers and the general public, in large part because its medicinal properties are becoming more widely recognized.  And although there is the potential for abuse of cannabis and it is still not considered legal by the federal government, cannabis has been deemed legal for medicinal or recreational use in many states.Cannabis produces a wide range of valuable secondary metabolites that include the cannabinoids, a unique class of terpenophenolic compounds found in its glandular trichromes. So far, out of 565 seconday compounds identified in Cannabis, 120 are cannabinoids. The plant’s behavioral and psychotropic effects are attributed to Δ9-Tetrahydrocannabinol (Δ9-THC) commonly known as THC. Other major cannabinoids include cannabigerol (CBG), cannabichromene (CBC), cannabidiol (CBD), tetrahydrocannabivarin (THCV) and cannabinol (CBN). The recent discoveries of medicinal properties of cannabis and the cannabinoids and their potential applications in the treatment of a number of serious illnesses such as glaucoma, depression, neuralgia, multiple sclerosis, Alzheimers, alleviation of symptoms of HIV/AIDS, cancer and childhood epilepsy, have given momentum to the quest for understanding the botany, chemistry and biology of this plant. In this session, the evolution of cannabis, its chemistry, potency as a psychotropic drug, and the current status and future development as a phytopharmaceutical will be discussed.


Introduction (H. Lata and T. M. Klein)

10:35  P-1 Evolution and Germplasm Conservation of Economic Groups of Cannabis
Ernest Small, Agriculture and Agri-Food Canada
11:10  P-2 Cannabis sativa L: Botany, Chemistry and Drug Development
Mahmoud A. ElSohly, The University of Mississippi
11:45  P-3 Cannabinoid Dosage Formulations – From the Field, Pharmacy, Dispensary and Street
Brian F. Thomas, RTI International
12:30 Discussion



Convener:      Maria M. Jenderek, ARS – USDA

10:30 am – 12:30 pm Plant Symposium Room 305

In literature, secondary metabolites are described as natural products, waste, phytopharmaceuticals, bioactive constituents or by-products of the primary metabolism. They occur in many plant genera and microorganisms in vivo and in vitro, and have complex chemical structures specific to the plants where they are found. Some of them are used in production of healthcare substances, food additives, flavor and color enhancers, nutraceuticals, fragrances and agrochemicals. Previously thought of as not being critical in plant functioning, they have a significant role in plant survival in their environment and in regulation of primary metabolic pathways. A large number of unique secondary metabolites have been identified; however, the function of many and their metabolic pathways are yet to be characterized. The session will discuss research on describing biosynthesis pathways of auxins and anthocyanins and the role of specific antioxidants in in-vitro plant cultures.

10:30 Introduction (M. M. Jenderek)
10:35  P-4 The Role of Local Auxin Biosynthesis in Plant Development
Anna Stepanova, North Carolina State University
11:00  P-5 Regulation of Anthocyanin Biosynthesis in the WD40-bHLH-MYB Complex-Programmed Arabidopsis Cells
Deyu Xie, North Carolina State University
11:25  P-6 A New Balancing Act: Melatonin and Serotonin as Mediators of Plant Morphogenesis
Lauren AE Erland, University of Guelph
11:50  P-7 Role of Antioxidants in In Vitro Plant Culture Systems
Praveen Saxena, University of Guelph
12:15 Discussion


Conveners:     Justin Colacino, University of Michigan School of Public Health, and Addy Alt-Holland, Tufts University

10:30 am – 12:30 pm Animal Symposium Room 306A

The number of assays designed to collect data from cell cultures and individual cells are rapidly expanding and are poised to revolutionize our understanding of cellular biology. However, there are also unanticipated technical and analytical challenges in the implementation of single cell analytics into typical in vitro biology workflows. Here we will highlight new applications in single cell analytics and heterogeneous cell cultures, ranging from cell health and viability to the generation high throughput ‘omics data while focusing on the generation of highly reproducible data. The first presentation will outline the utility of single cell assays, including flow cytometry and single cell transcriptomics, to identify and characterize rare cell populations, such as stem cells or circulating tumor cells. The second presentation will focus on ways to improve the reproducibility of cell-based assays and approaches to multiplex distinct cell health measurements to simultaneously analyze multiple parameters in a given sample of cells.

10:30 Introduction (J. Colacino and A. Alt-Holland)
10:35 A-1 Single Cell Transcriptomics: Unraveling Heterogeneity in Environmental Health and Cancer
Justin A. Colacino, University of Michigan School of Public Health
11:10 A-2 Treating Cells as Reagents to Achieve Assay Reproducibility
Terry Riss, Promega Corporation
11:45 A-3 In Vitro Methods for Measuring Cell Health in Real-Time
Terry Riss, Promega Corporation
12:20 Discussion


Convener:       Paula M. Pijut, USDA Forest Service

1:30 pm – 3:00 pm Plant Symposium Room 305

Research on in vitro ploidy manipulation of nursery and ornamental woody species, as well as ornamental and bioenergy grasses, and the use of molecular markers to assess hybridity will be discussed. In addition, a high-throughput transformation system in Populus that enables the testing of large numbers of candidate genes will be described. This system has been used to produce transgenic lines representing over 600 gene constructs in a joint program focused on understanding and eliminating biomass recalcitrance in order to improve biofuel yields.

1:30 Introduction (P. M. Pijut)
1:35 P-8 In Vitro Ploidy Manipulation for Ornamental and Bioenergy Crop Improvement
Darren Touchell,
North Carolina State University
2:10 P-9 High Throughput Transformation Systems
J. Eric Gulledge, ArborGen, Inc.
2:55 Discussion


Conveners:     Annie Saltarikos, Monsanto Company, and Marceline Egnin, Tuskegee University

1:30 pm – 3:00 pm Plant Symposium Room 306BC

As the world’s population continues to rise and the amount of arable farmland remains static, there is a vested interest to continue to develop high-yielding crops for food and feed, while using less inputs. The last several decades have seen a rapid adoption and deployment of biotechnology practices across the major crop species to deliver genes or favorable traits (both foreign and plant-derived) to increase yield, quality, and provide resistance to both biotic and abiotic stresses. Alongside these advances, best practices in biosafety and regulatory pathways guiding the risk assessment of GM crops to their commercialization have been gained since the 90’s.  However, public perception of these technologies’ safety has been mixed, and the regulatory trigger pathway for these products is complex but limited in its current practices. Most recently, the advent and relative ease and speed of use of genome engineering tools such as Clustered Regularly Interspersed Short Palindromic Repeats (CRISPR)-Cas9 has dramatically cut down the previous bottlenecks of having to provide protein engineering to target specific DNA sequences within the genome; thus lowering the barrier to rapid technical advancements in this field of precision genome engineering for crops. However, just as quickly as the number of technical advancements in this emerging field arise, so do the questions and challenges of how these precision engineered plant products will be further regulated and perceived by the public in terms of safety and acceptance. The advantages of genome editing are multi fold, as it can provide non-transgenic methods of creating desirable traits through direct manipulation of native alleles, as well as can accelerate deployment of genome edited products across crops through potentially shortening US regulation oversight triggers that have limited deployment of traditional GM crops in distinct regulation boundaries. This sessions’ speakers from industry, Institutions, academia, and government will share both the current regulatory and biosafety landscape for genome edited products as well as discuss the challenges and best regulatory practices.

1:30 Introduction (A. Saltarikos and M. Egnin)
1:35 P-10 Biotech and Genome Editing Regulations
Alan McHughen, University of California – Riverside
2:00 P-11 Engineering the DNA of Our Governance Systems: Biosafety Best Practices and Issues for GMOs
Todd Kuiken, North Carolina State University
2:25  P-12 Regulatory Considerations for Genome Editing in Crops
Miguel Vega-Sanchez, Monsanto Company
2:50 Discussion


Moderator:      Addy Alt-Holland, Tufts University

1:30 pm – 3:00 pm Animal Contributed Paper Session Room 306A
1:30 Introduction (A. Alt-Holland)
1:35 A-1003 In  Bioassays, Blind Faith Is a Fool’s Errand-Run the Controls Every Time
John W. Harbell,
JHarbell Consulting LLC
1:55 A-1004 An Adventure in Serum-free Cell Culture
Denry Sato, Manzanar Project Foundation
2:15  A-1005 Gap Junctional Intercellular Communication, the Archtypal Cell Phone of Tissues, Is an Excellent Biomarker for Assessing Environmental Stressors and Chemopreventive Compounds
Brad L. Upham, Michigan State University, Iva Sovadinova, and Pavel Babica
2:35  A-1006 Intrinsic Electrical Signal Characteristics of Rat Embryonic Cortex Tissue
Nurdan Ozkucur, Tufts University
2:55 Discussion


Conveners:     Vivian R. Dayeh, University of Waterloo, and Cynthia L. Goodman, USDA-ARS-BCIRL

3:00 pm – 5:00 pm Animal Symposium Room 306A

In vitro approaches play an important role in dissecting and understanding complex relationships within agricultural and environmental systems. Continuously replicating cell lines as well as primary cell cultures from an array of species offer meaningful insights into physiological and toxicological impacts of agricultural, industrial, and other human-based inputs into the environment. Likewise, cell cultures are valuable tools for both screening and mechanism of action studies in AgDiscovery programs aimed at developing pest-specific products. Our session will showcase cell-based assays that are being used to investigate xenobiotic impacts on human lung function, amphibian immunity, insect digestive processes, and invertebrate cell viability.

3:00 Introduction – Environmental Presentations (V. R. Dayeh)
3:05 A-4 Modeling Human Lung Fibroblast Responses in Asthma and Airway Disease
Jennifer Leigh Ingram, Duke University Medical School
3:30 A-5 Expanding the Xenopus laevis Invitrome: Establishing and Characterizing Cell Lines For Use in Examining Host-Pathogen-Environment Interactions
Barb Katzenback, University of Waterloo
3:55 Introduction – Agricultural Presentations (C. L. Goodman)
4:00 A-6 Insights on the Mode of Action of Insecticidal Proteins from Bacillus thuringiensis Toxins from Cell Cultures
Juan Luis Jurat-Fuentes, University of Tennessee
4:25 A-7 Establishing Cell Lines from Pest Insects: Studies of Prostaglandin Actions
David Stanley, USDA, ARS, BCIRL
4:50 Roundtable discussion including all speakers


Moderator: Jeffrey Beringer, Dow AgroSciences

Panel of Plant Biotechnology Experts Evaluating the Contestants: Barbara M. Reed, USDA-ARS Retired; Terry Hu, DuPont Pioneer, and Sergei F. Krasnyanski, North Carolina State University

3:00 pm – 5:00 pm Plant Contributed Paper Competition Room 305

To support the Society’s vision to encourage education and scientific informational exchange and recognize outstanding students, the Plant Biotechnology Section is pleased to announce the 2017 Student Oral Presentation Competition. Student competition finalists were selected based on the quality of the abstracts. A panel of judges will evaluate the presentations at the meeting. Criteria for the evaluation include experimental design, data analysis, proper interpretation of the results, originality of the study, technical difficulty, appearance and ability of the student candidate to present it. Winners will be presented with a certificate and a cash award at the meeting.

3:00 P-6 A New Balancing Act: Melatonin and Serotonin as Mediators of Plant Morphogeneis
Lauren A. E. Erland,
University of Guelph, and Praveen K. Saxena
3:20 P-1000 Effect of Dessication Stress and Subsequent Recovery on the in Vitro Growth of the Epiphytic Orchid Dendrophylax lindenii
Jameson Coopman, University of Florida, and Michael Kane
3:40 P-1001 Soybean (Glycine max) Promoter Characterization Through CRISPRi
Andika Gunadi, The Ohio State University, and John J. Finer
4:00 P-1002 A CRISPR Toolkit for Plants
Jacob W. Hoyle, University of Georgia, Peter LaFayette, and Wayne Parrott
4:20 P-1003 This abstract has been withdrawn
4:40 P-1004 Enhancement of Halotolerance in Fremyella diplosiphon by Electroporation-mediated Overexpression of Malate Dehydrogenase Gene
Behnam Tabatabai, Morgan State University, AnithaChristy Arumanayagam, and Viji Sitther


Moderator:      Piero Barone, Dow AgroSciences

3:00 pm – 5:00 pm Plant Contributed Paper Session and
Plant Contributed Paper Competition
Room 304
3:00 P-1005 Global Scale Investigation of Soybean MicroRNAs Responsive to Soybean Cyst Nematodes Infection
Bin Tian, Kansas State University, Shichen Wang, Charles D. Johnson, Guiliang Tang, and Harold N. Trick
3:20 P-1006 It’s About Time; Rapid and Simple Maize Transformation
George J. Hoerster,  DuPont/Pioneer, Keith Lowe, Keith Lowe, Mauricio La Rota, Craig Hastings,  Ning Wang, Emily Wu,  Ajith Anand, Maren Arling,  Brian Lenderts, Mark Chamberlin, Todd Jones, and William Gordon-Kamm
3:40 P-1007 Under the Radar: Enhanced Legume Transformation by Altering the Host Receptor – Agrobacterium Effector Interactions
Timothy M Chappell, University of Georgia, Brian H Kvitko, and Wayne Parrott
4:00 P-1008 Employing Linear Minimal DNA Expression Cassettes for Sugarcane Genetic Transformation Using Biolistics Approach
Muhammad Sohail Akram, Government College University, and Javed Iqbal

Panel of Plant Biotechnology Experts Evaluating the Contestants: Prakash Kumar, National University of Singapore, Dennis Gray, University of Florida, and Geny Anthony, Dow AgroSciences

To support the Society’s vision to encourage education and scientific informational exchange and recognize outstanding post docs, the Plant Biotechnology Section is pleased to announce the 2017 Post-Doctoral Oral Presentation Competition. Postdoctoral competition finalists were selected based on the quality of the abstracts. A panel of judges will evaluate the presentations at the meeting. Criteria for the evaluation include experimental design, data analysis, proper interpretation of the results, originality of the study, technical difficulty, appearance and ability of the post-doctoral candidate to present it. Winners will be presented with a certificate and a cash award at the meeting.

4:20 P-1009 Improving Agrobacterium-mediated Transformation of Cowpea (Vigna unguiculata L.)
Zhifen Zhang, University of Georgia, Kathleen Monfero Marasigan, Yinping Guo, and Peggy Ozias-Akins
4:40 P-1010 Enhanced Production of Prenylated Stilbenoids and Elucidation of Their Biosynthetic Pathway in Hairy Root Cultures of Peanut
Tianhong Yang, Arkansas State University, Lingling Fang, Keithanne Mockaitis, and Fabricio Medina-Bolivar


Program Chair:           Addy Alt-Holland, Tufts University

5:00 pm – 6:00 pm Opening Ceremony Room 306BC

Conveners:  Addy Alt-Holland, Tufts University, and Dwight T. Tomes

5:00 Welcome and Opening Remarks:
Dwight T. Tomes, President, Society for In Vitro Biology
5:05 2017 Fellow, Distinguished Scientist, and Young Scientist Award Recipients (Awards to be presented at Section Meetings)

Distinguished Scientist: Allan Wenck, Bayer CropScience
Fellow: Miho Furue, National Institutes of Biomedical Innovation, Health and Nutrition
Kan Wang, Iowa State University
Young Scientist: Sukhpreet Sandhu, Bayer


5:15 2017 Distinguished Service Award Presentations
(Awards to be presented by Dwight Tomes)

Vadim Beilinson, AgBiome
Vivian Dayeh, University of Waterloo
Michael J. Fay, Midwestern University
Ted Klein, Pioneer Crop Genetics
Sergei Krasnyanski, North Carolina State University
Qiudeng Que, Syngenta
Pon Samuel, Dow Agrosciences
Mary Welter

5:30 2017 Lifetime Achievement Award Presentations
(Introduction by Sandra Schneider; Acknowledgement by Dr. Sato to follow)
Denry Sato, Manzanar Project Foundation
5:45 (Introduction by Baochun Li, Acknowledgement by Dr. Phillips to follow)
Gregory C. Phillips, Arkansas State University
6:00  Adjourn
6:00 pm – 7:30 pm Exhibits and Posters Ballroom C

Sunday, June 11
Odd Poster Authors will be present
6:30 pm – 7:30 pm


Conveners:     Elena Arthur, North Carolina Central University, and Whitney Harchenko, Montana State University

7:30 pm – 9:30 pm Education Symposium Room 304

Public speaking is frequently ranked as the number one fear for adults — coming in higher than fear of death! Yet speaking publicly is increasingly becoming a requirement for many jobs. Luckily, effective public speaking is not magic. There are specific skills that enhance presentation delivery, strategies for program development, and tips for converting speaker anxiety into energy. This interactive workshop provides participants with practical tools for public speaking in any setting.

7:30 Introduction (E. Arthur and W. Harchenko)
7:35 E-1 Persuasive Presentations: Tips and Techniques for Public Speaking
Cally Ritter, AllOne Health
8:35 Discussion