Monday, June 12

For your viewing convenience, the 2017 In Vitro Biology Meeting Final Program has been broken down by day.

Daily Program
2017 In Vitro Biology Meeting
Saturday
June 10
Keynote Symposium Plant Contributed Papers
Sunday
June 11
Plenary Symposia Animal Posters
Monday
June 12
Animal Symposia and Workshops Education Silent Abstracts
Tuesday
June 13
Education Symposia and Workshops Plant Posters
Wednesday
June 14
Plant Symposia and Workshops Abstract Issue Index
Poster Sessions Animal Contributed Papers Late Submission Abstracts and Index

Daily Program-at-a-Glance

Time Event Location
7:00 am – 6:00 pm Registration Ballroom C Lobby
10:00 am – 3:30 pm

7:30 pm – 10:00 pm

Exhibits and Posters Ballroom C
Morning 7:00 am – 12:30 pm
7:00 am – 8:00 am Publications Committee Meeting Governors Board Room
8:00 am – 10:00 am Microbiome Challenges to Scale Production Room 306BC
10:00 am – 10:30 am Coffee Break Ballroom C
Development Committee Meeting University AB
Awards Committee Meeting Governors Board Room
10:30 am – 12:00 pm Robert H. Lawrence, Jr. Keynote Symposium

10:30 Welcome and Opening Remarks:
10:40 Robert H. Lawrence Jr. Keynote Presentation
11:40 Questions and Answers
11:55 Group photo with Dr. Atala and student attendees
Room 306BC
12:00 pm – 12:30 pm Robert H. Lawrence, Jr. Keynote Symposium Reception Ballroom C
Afternoon 12:30 pm – 6:00 pm
12:30 pm – 1:30 pm Student Networking Luncheon: Creating Winning Resumes and CVs Room 304
Long Range Planning Committee Meeting University AB
Refreshment Break Ballroom C
1:30 pm – 2:30 pm In Vitro Animal Cell Sciences Student and Post Doctoral Oral Presentation Competition Room 306A
Interactive Poster Sessions:

Abiotic Stress
Micropropagation I

Ballroom C
 2:30 pm – 3:30 pm Even Poster Authors will be present Ballroom C
3:30 pm – 5:00 pm In Vitro And Silico Databases And Analysis Room 306A
Non-Competitive Student Oral Presentations Room 304
Regulatory Protocols for Transgenic Crops – Assessing Pollen-Mediated Transgene Flow in Annual and Perennial Crops Room 306BC
Workshop on Cannabis Best Practices and Regulation Room 305
5:00 pm – 6:00 pm In Vitro Animal Cell Sciences Contributed Papers Room 306A
Plant Responses To Abiotic Stress Room 306BC
Genetic Engineering II Room 305
Evening 6:00 pm – 10:00 pm
6:00 pm – 7:30 pm In Vitro Animal Cell Sciences Section Business Meeting Room 306A
Plant Biotechnology Section Business Meeting Room 306BC
7:30 pm – 10:00 pm Joint Sections Social Ballroom B

Monday, June 12

7:00 am – 6:00 pm Registration Ballroom C Lobby

MICROBIOME CHALLENGES TO SCALE PRODUCTION

 

Conveners:    Pooba Ganeshan, Saskatchewan Research Council, and Mae Ciancio, Midwestern University

8:00 am – 10:00 am Plenary Symposium Room 306BC

This plenary session is poised to address the major issues concerning the microbiome in human as well as agricultural applications. Dr. Chad Keyser from AgBiome will present exciting news regarding their application of specific microbes in the ongoing quest to protect plants from insect and fungal devastation in the United States as well as Sub-Saharan Africa. Dr. M. Andrea Azcarate-Peril, Director of the Microbiome Core Facility at the University of North Carolina School of Medicine will review emerging technologies, such as engineered organoids, high throughput screening, and microfluidics assays, and their application to understanding the host microbial alterations produced by pro- and prebiotics in the maintenance of health and prevention of disease. Dr. Jose M. Bruno-Barcena, from the department of Plant and Microbial Biology, North Carolina State University, will complete the plenary session by discussing the major benefits of packed-bed reactors, dynamics culturing, and stress-directed in vitro community microbiota selection in the product ion of biomass and metabolites of agrochemical interest.

8:00

Introduction (P. Ganeshan and M. Ciancio)

8:05  PS-3 The Path to Product: Solving Agriculture’s Greatest Challenges One Microbe at a Time
Chad Keyser, AgBiome, Inc.
8:40  PS-4 Current and Emerging Technologies in the Study of Beneficial Modulators of the Gut Microbiome
M. Andrea Azcarate-Peril, University of North Carolina
9:15 PS-5 Packed-bed Reactors, Dynamic Culturing, and Stress-directed Microbiome Selection; a Case Study of Agrochemicals Soil Biodegradation
Jose Bruno-Barcena, North Carolina State University
9:50 Discussion
10:00 am – 10:30 pm Coffee Break Ballroom C

ROBERT H. LAWRENCE, JR. KEYNOTE SYMPOSIUM

10:30 am – 12:00 pm Keynote Symposium Room 306BC
10:30 Welcome and Opening Remarks:
Dwight T. Tomes, President, Society for In Vitro Biology
10:40 KS-1 Introduction (J. Z. Gasiorowski)

Regenerative Medicine: Current Concepts and Changing Trends
Anthony Atala
Director and Chairman of the Wake Forest Institute for Regenerative Medicine
W. Boyce Professor and Chair of Urology at Wake Forest University

11:40 Questions and Answers
11:55 Group photo with Dr. Atala and student attendees
12:00 Adjourn

 

12:00 pm – 12:30 pm ROBERT H. LAWRENCE, JR. KEYNOTE SYMPOSIUM RECEPTION
Honoring Keynote Speaker, Anthony Atala
Ballroom C

STUDENT NETWORKING LUNCHEON: CREATING WINNING RESUMES AND CVS

Conveners:    Elena Arthur, North Carolina Central University, and Whitney Harchenko, Montana State University

12:30 pm – 1:30 pm Education Workshop Room 304

Whether it is for acceptance into graduate school or to land your dream job, a resume or CV is the best tools we can employ to present ourselves to the world. This workshop will give a framework on how to build an effective resume and CV. A career expert will render a lecture on practical ways to create a resume and CV and how to make it stand out from everyone else. Students will learn tools to give them the confidence for building their own resume or CV. After the presentation, they will also have the privilege to have a one-on-one resume/CV consultation with a career expert. Appointments will be available on a first-come first-serve basis so students must sign up when they first register upon arrival.

12:30 Introduction (E. Arthur and W. Harchenko)
12:35  E-2 Interactive Session: Creating Winning Resumes and CVs
Patrick Brandt, University of North Carolina at Chapel Hill

IN VITRO ANIMAL CELL SCIENCES STUDENT AND POST DOCTORAL ORAL PRESENTATION COMPETITION

Moderators:    Addy Alt-Holland, Tufts University, and Kolla Kristjansdottir, Midwestern University

1:30 pm – 2:30 pm Animal Contributed Paper Competition Room 306A

Panel of In Vitro Animal Cell Sciences Experts Evaluating the Contestants: Addy Alt-Holland, Tufts University, Mae Ciancio, Midwestern University, Justin Colacino, University of Michigan Medical School, Michael Dame, Barbara B. Doonan, New York Medical College, Michael J. Fay, Midwestern University, University of Michigan Medical School, John W. Harbell, JHarbell Consulting, LLC, Kolla Kristjansdottir, Midwestern University, and Brad L. Upham, Michigan State University

The In Vitro Animal Cell Sciences Section of the Society for In Vitro Biology is pleased to announce the 2017 Student and Post-Doctoral Oral Presentation Competition. This competition serves as a platform to recognize the research work and achievements of outstanding students and post-docs, and aims to encourage education and the exchange of scientific information during the conference. Three competition finalists were selected based on the quality of the abstracts and the merit of the scientific findings. A panel of expert judges will grade the presentations at the Oral Presentation Competition session. Criteria that would be evaluated include the experimental design, data analysis, proper interpretation of the results, originality of the study, technical difficulty, appearance and ability of the finalist to explain the research work and adherence to the allocated time for the presentation. Winners will be presented with a certificate and a cash award at the meeting.

1:30 A-1000 Scavenging for Bacteria: Identification and Characterization of Rainbow Trout MARCO
Sarah J. Poynter, University of Waterloo, Andrea Monjo, Gabi Micheli, and Stephanie DeWitte-Orr
1:50 A-1001 Characterizing Functional Differences in Sea Anemone Hsp70 Isoforms Using Budding Yeast
Shawn J. Waller, University of North Carolina – Charlotte, Adam M. Reitzel, and Andrew W. Truman
2:10 A-1002 Enhanced Directional Axon Outgrowth of Peripheral Nerve Fibers Using Submicron Topgraphic Cues and Live Cell Imaging
Robert Garcia, Midwestern University, Harsh Sharthiya, Sarah Veen, Claire Lu, Nil Patel, Kolbrun Kristjansdottir, Michele Fornaro, Paul F. Nealey, and Joshua Z. GasiorowskI

ABIOTIC STRESS

Moderator:      Samson Prabhakar Nalapalli, Syngenta Biotechnology Inc.

1:30 pm – 2:30 pm Plant Interactive Poster Session Ballroom C
P-2000 C-terminally Encoded Peptides as Candidates for Abiotic Stress Response in Plants
Sumita Kumari, Sher-e-Kashmir University of Agricultural Sciences and Technology, Jebi Sudan, Devyani Sharma, and Ananda Mustafiz
P-2001 Overexpression of AhCuZnSOD and Pyramiding of AhCuZnSOD with the AhcAPX Gene Enhance Salinity and Dehydration Stress in Brassica juncea
Neelam Prabha Negi, Jawaharlal Nehru University, Vinay Sharma, and Neera Bhalla Sarin
 P-2002 RNAi Knockdown of Potato Genes Crucial for Potato Virus Y Replication
Whitney Harchenko, Montana State University, Myron Bruce, and Jessica Rupp

MICROPROPAGATION I

Moderator:      Dharam Sharma, Duarte Dry Creek Laboratories

1:30 pm – 2:30 pm Plant Interactive Poster Session Ballroom C
P-2006 Establishment of Callus Cultures from Different Axenic Leaf Explant Types of Lentisk (P. lentiscus L.)
Veysel Süzerer, Bingől University , Elif Demir, Ayşe Hoşer, Hilal Surmuş Aşan, Engin Tilkat, Abdulselam Ertaş, and Ahmet Onay
P-2007 Effect of Plant Growth Regulators on Micropropagation of Commercially Important Pineapple Cultivars
Virendra M. Verma, Micronesia Plant Propagation Research Center
P-2008 In Vitro Propagation of Bougainvillea glabra Choise Variegate Variety by Somatic Embryos
C. M. Rodriguez-Salazar, Instituto Politécnico Nacional, and S. Evangelista-Lozano
P-2009 Micropropagation of Miscanthus x giganteus ‘Illinois’: Improved Regeneration Capacity of Callus Cultures and Optimized Microrhizome Development of In Vitro Plantlets
Cassandra Doll Downey, University of Guelph, and Andrew Maxwell Phineas Jones

Monday, June 12
Even Poster Authors will be present
2:30 pm – 3:30 pm

IN VITRO AND SILICO DATABASES AND ANALYSIS

Convener:       Dawn R. Applegate, RegeneMed, Inc

3:30 pm – 5:00 pm Animal Symposium Room 306A

Advances in tools for modeling the contributions of environment and genetics in human disease are enabling improved health, nutrition, food security and crop protection. These databases and analysis tools integrate complex and disparate data sets into predictive and integrative biology platforms to accelerate agroscience, nutritional science, therapeutic development, and personalized medicine. In vitro and in silico platforms from government, academic and industrial labs encompassing plant and organotypic cell cultures, high throughput and high content screening, ‘omic data, and predictive analytics with application to agriculture, nutrition, therapeutics and medicine will be presented.

3:30 Introduction (D. R. Applegate)
3:35 A-8 Databases and Analysis Tools Supporting Toxicology in the 21st Century
Nisha S. Sipes, NIEHS
3:55 A-9 The Application of Global Metabolomics to Plant Sciences
Danny Alexander, Metabolon, Inc.
4:15 A-10 Biomarker Discovery in Human Trials Through the Analysis of Metabolomic, Proteomic, and RNA-seq Data
Matthew Wampole,
Clarivate Analytics
4:35  A-18 Integrative Approaches to Defining the State and Architecture of the Breast Cancer Kinome
Shawn Gomez, UNC/NC State University Joint Department of Biomedical Engineering
4:55 Discussion

NON-COMPETITIVE STUDENT ORAL PRESENTATIONS

Moderators:  Elena Arthur, North Carolina Central University, and Whitney Harchenko, Montana State University

3:30 pm – 5:00 pm Education Workshop Room 304

Oral presentations are an intimidating and difficult part of graduate school for many students.  However, being able to communicate the results of experiments in an oral presentation is an essential skill as a scientist.  This session is a perfect opportunity for graduate students to practice their oral presentation skills in a low pressure setting and to receive valuable feedback from SIVB members.

3:30 Introduction (E. Arthur and W. Harchenko)
3:35  P-3022 Regulation of Cell Wall Strength and Salt Tolerance by The Arabidopsis MUR4
Omar Zayed, Purdue University, and Chunzhao Zhao
3:55  A-3009 Maltooligosaccharide Chemosensation by Intestinal Enteroendocrine L-Cells Regulate the Endogenous Release of Gut Hormones and Glucose Homeostasis
Marwa El Hindawy, Purdue University, Choon Young Kim, and Bruce R. Hamaker
4:15  P-3028 Further Studies of Putative Gametic Calli from Soybean Anther Cultures
Martina Garda and Bretton Hale, Arkansas State University, M. Lowe, S. Goodling, N. Rao, K. Bade, and G. C. Phillips
4:35  TBD
4:55 Discussion

REGULATORY PROTOCOLS FOR TRANSGENIC CROPS – ASSESSING POLLEN-MEDIATED TRANSGENE FLOW IN ANNUAL AND PERENNIAL CROPS

SESSION SUPPORTED BY AN EDUCATIONAL GRANT FROM
USDA – BIOTECHNOLOGY RISK ASSESSMENT GRANT PROGRAM

Conveners:     Sadanand A. Dhekney, University of Wyoming, and Harold N. Trick, Kansas State University

3:30 pm – 5:00 pm Plant Symposium Room 306BC

The widespread adoption and cultivation of GM crops has raised a specter of opposition over concerns regarding the unintended consequences of genetic modification on human and animal health and the ecosystem. A major concern triggering a worldwide debate has been the potential impact of transgene flow to wild/weedy relatives or non-transgenic crops grown in the vicinity of  their genetically modified counterparts . Transgene flow into the environment depends on several factors including the specific crop in question, the presence of sexually compatible crops and wild species in the vicinity and the competitive nature of the introduced trait. Transgene flow can occur via pollen-mediated gene flow or seed-mediated gene flow. Pollen-mediated transgene flow may occur when pollen from transgenic plants fertilize flowers from non-transgenic plants via wind or insect pollination. The potential for gene flow through pollen depends on several factors including the quantity of pollen produced by GM plants, the viability of pollen, dispersal by wind, birds and animals, ability of the pollen to compete with native pollen to pollinate wild relatives and the sexual compatibility of the wild relatives with the commercial crop species. The current session will discuss pollen-mediated transgene flow in annual, perennial and forestry species and methods to mitigate transgene flow into non-transgenic crop cultivars and wild relatives.

3:30 Introduction (H. N. Trick and S. A. Dhekney)
3:35 P-13 Bentgrass Goes AWOL: A Story of Gene Flow, Research and Regulation
Carol Auer, University of Connecticut
3:55 P-14 RNAi and Gene Editing as Tools for Containment of Genetically Engineered and Exotic Forest Trees
Steven Strauss, Oregon State University
4:15 P-15 Evaluating Unintended Open Reading Frames in Genetically Modified Plants
Hope Hart, Syngenta
4:35 P-16 Regulatory and Risk Assessment Issues with Environmental Releases of Genetically Engineered Plants
John Cordts, Cordts Consulting, LLC
4:55 Discussion

WORKSHOP ON CANNABIS BEST PRACTICES AND REGULATION

Conveners:     Mary Welter and William Graham, Pure Food Gardening/ Microclone Propagation

3:30 pm – 5:00 pm Plant Workshop Room 305

Modern tissue culture, horticultural, processing, and chemical and genetic testing technologies are rapidly being adopted by the Cannabis industry. This is in large part due to the shift in legal status for the cultivation, sale and use of Cannabis. Recreational and/or medical use which has been deemed legal by 28 states (along with District of Columbia) with the anticipation that as many as seven additional states could follow in 2017. Although the Federal Government of the US still considers Cannabis illicit, all of Canada has had legalized medical Cannabis since 2001. Despite the discrepancies between state and federal law, the Cannabis industry is rapidly becoming ‘professionalized’ and is attracting significant venture capital investment and interest from the scientific community. There are substantial opportunities to investigate and apply existing or new strategies for stable and consistent plant production and to apply modern molecular tools to facilitate genotype identification. This workshop hopes to initiate dialog and discussion concerning the efforts that will be needed to optimize and standardize some of the important aspects of the industry, including micropropagation, genotyping, and regulation of the crop.

3:30 Introduction (M. Welter)
3:35 P-17 Cannabis In Vitro Propagation – A New Crop in an Experienced Industry
William Graham, Pure Food Gardening/ Microclone Propagation
4:00 P-18 Operational Optimization Through Quality Management Systems
Tom Shipley, Tweed
4:25 P-19 Names, Strains, and Claims, Oh My! Incorporating the Use of Genetic Analysis in a Budding Industry
Anna Schwabe, University of Northern Colorado
4:50 Discussion

IN VITRO ANIMAL CELL SCIENCES CONTRIBUTED PAPERS

Moderator:      Joshua Z. Gasiorowski, Midwestern University

5:00 pm – 6:00 pm Animal Contributed Paper Session Room 306A
5:05 A-1007 Function of rBC2LCN Lectin-recognizing Glycoprotein-positive Cells in Squamous Cell Carcinoma Cell Lines
Hirotaka Nakatao, Hiroshima University Graduate School of Biomedical & Health Sciences, Eri Akagi, Atsuko Hamada, Sachiko Yamasaki, Shigeaki Toratani, and Tetsuji Okamoto
 5:20  A-1008 Endogenous Epitope Tagging of Hsp70 Using CRSIPR/Cas9 Genome Editing
Nitika, University of North Carolina at Charlotte, and A. W. Truman
 5:35  A-1009 The Influence of Defined Extracellular Geometries on Mammalian Transgene Expression
A. J. Youssef, Midwestern University, S. C. Veen, and J. Z. Gasiorowski
4:40 P-1010 Discussion

PLANT RESPONSES TO ABIOTIC STRESS

Conveners: Prakash P. Kumar, National University of Singapore, and Hong Luo, Clemson University

5:00 pm – 6:00 pm Plant Symposium Room 306BC

Abiotic stresses, such as water deficiency, salinity, and nutrition starvation, have been the major limiting factors in plant growth and will soon become even more acute as desertification covers increasingly more of the world’s terrestrial area. Drought and salinity are already widespread in many regions, and are expected to cause serious salinization of more than 50% of all arable lands by the year 2050. They are therefore among the most important components in modern agriculture significantly impacting crop productivity. Genetic improvement of crops for new cultivars highly tolerant to extreme environmental conditions is the key in sustainably producing enough food feeding a burgeoning world population. Plants cope with environmental stress through various mechanisms involving multiple physiological, molecular and cellular processes. Agricultural biotechnology manipulating expression of genes involved in plant stress response and aimed at crop genetic improvement for enhanced adaptation to abiotic stresses has huge economical impacts. This symposium will focus on recent progress in deciphering molecular mechanisms of plant abiotic stress response and exploring biotechnology approaches for use in crop genetic improvement for enhanced plant performance under adverse environmental conditions.

5:00 Introduction (P. P. Kumar and H. Luo)
5:05 P-20 The Molecular Mechanism Behind Hydrophobic Barrier Formation to Confer Salt Tolerance in Plants
Prakash P. Kumar, National University of Singapor
5:20 P-21 Small Molecules, Big Impacts – MicroRNAs in Perennial Grass Development and Stress Response
Hong Luo, Clemson University
5:35 TBD
Eduardo Blumwald, University of California – Davis
5:50 Discussion

GENETIC ENGINEERING II

Moderator:     Sergei Krasnyanski, North Carolina State University

5:00 pm – 6:00 pm Plant Contributed Paper Session Room 305
5:00 P-1011 Development of a Protoplast System for Non-transgenic, Targeted Genome Editing in S. tuberosum
Michele Fossi, H. M. Clause/ University of California Davis, Kirk Amundson, Sophia Jinata, and Luca Comai
5:15 P-1012 Development of Agrobacterium-mediated Transformation of Physalis peruviana and Application of CRISPR/Cas9 Genome Editing
Joyce Van Eck,The Boyce Thompson Institute, Kerry Swartwood, Zachary H. Lemmon, Justin Dalrymple, and Zachary B. Lippman
5:30 P-1013 Generation of Glyphosate Tolerant Cassava Plants Through CRISPR/CAS9-mediated Gene Editing
Raj Deepika Chauhan, Donald Danforth Plant Science Center, KWS Gateway Research Center, Aaron Hummel, Thomas Cermak, Colby Starker, Rebecca Bart, Daniel Voytas, and Nigel Taylor
5:45 P-1014 Opportunities for Plant Engineering to Promote Sustainable Food Systems
Elizabeth Specht, The Good Food Institute, and Christie Lagally

6:00 pm – 7:30 pm IN VITRO ANIMAL CELL SCIENCES  SECTION MEETING Room 306A
6:00 pm – 7:30 pm PLANT BIOTECHNOLOGY SECTION MEETING Room 305
7:30 pm – 10:00 pm JOINT SECTIONS’ SOCIAL Ballroom B and C