Tuesday – June 14, 2016

For your viewing convenience, the 2016 World Congress on In Vitro Biology Final Program has been broken down by day.

Daily Program
Abstracts & Posters
Keynote Speaker Index Plant Symposia & Workshops
Saturday, June 11 Plenary Symposia Plant Contributed Papers
Sunday, June 12 Keynote Symposium Plant Posters
Monday, June 13 Animal Posters Education Posters
Tuesday, June 14 Joint Symposia Animal & Education Contributed Papers
Wednesday, June 15 Animal Symposia & Workshops Addendum Booklet
International Conference Symposia


Daily Program-at-a-Glance

Time Event Location
7:00 am – 5:00 pm Registration Bayview Foyer
10:00 am – 3:30 pm Exhibits and Posters Nautilus
Morning 7:00 am – 12:30 pm
7:00 am – 8:00 am In Vitro – Animal  Editorial Board Meeting Marina 4
Student Affairs Breakfast Harbor Island 1
8:00 am – 10:00 am Infinite Potential of Stem Cells Harbor Island 2
10:00 am – 10:30 am Coffee Break Nautilus
Education Committee Meeting Marina 1
Constitution and Bylaws Meeting  Marina 4
10:30 am Closing of America’s Finest Silent Auction Nautilus
10:30 am – 12:30 pm Epigenetics, Somaclonal Variation and the Cellular Environment Harbor Island 3
Human Cell Models for Disease Marina 6
Rapid Model System for Idea Testing in Crop Plants Harbor Island 2
Afternoon 12:30 pm – 5:00 pm
12:30 pm Announcement of America’s Finest Silent Auction Winners Bayview Foyer
12:30 pm – 1:30 pm 2017 Program Planning Committee Meeting Marina 4
1:30 pm – 2:30 pm Interactive Poster Sessions
In Vitro Animal Cell Sciences
Biotechnology II
2:30 pm – 3:30 pm Odd Poster Authors will be present Nautilus
3:30 pm – 5:00 pm Posters to be removed from Exhibit Hall Nautilus
Organoids and Other Three-Dimensional In Vitro Tissues – One Step Closer to Personalized Treatment Marina 6
Functional Food Harbor Island 2
Biotechnology and Genetic Engineering Harbor Island 3
Evening 5:00 pm – 10:00 pm
5:00 pm – 5:45 pm SIVB Business Meeting
(All Members Are Urged to Attend)
Student Award Presentations
Executive Conference Room 2A & 2B
6:15 pm – 10:00 pm An Evening Cruise on the San Diego Bay
Admittance by Advance Ticket Holders Only
San Diego Bay

Tuesday, June 14

7:00 am – 5:00 pm Registration Bayview Foyer


Conveners:   Paul Price, William Gordon-Kamm, Pioneer Hi-Bred International Inc., and Tetsuji Okamoto, Hiroshima University

8:00 am – 10:00 am Plenary Symposium Harbor Island 2

In the very near future we will understand much of the potential of stem cells.  Stem cells will help us understand cellular differentiation and the formation of tissues and organs.   Differentiated human stem cells will offer In Vitro systems for drug studies.  The use of human stem cells will include cellular therapy for the treatment of spinal cord injury, macular degeneration, heart disease, burns, diabetes, stroke and osteo- and rheumatoid arthritis.  It is the results of animal studies that have led us today to multiple human clinical trials. This session will update the audience on where we are and where we are going. In plants, much progress has been made in understanding the molecular control of stem cells in the meristem, and new facets of this control network continue to be unraveled.  However, in addition to understanding the underlying mechanisms, the meristem transcription factor WUS is being successfully used to redirect cell fates in corn, creating de novo “meristems” that are having positive implications for improving corn transformation.  Updates on these areas will be provided.

8:00 Introduction (P. Price, W. Gordon-Kamm and T. Okamoto)
8:05 PS-8 Establishment and Characterization of Normal and Disease-specific of Human IPSC in Serum-, Integration- and Feeder-free Cultures
Sachiko Yamasaki, Hiroshima University
8:30 PS-9 Transplantation of Stem Cell-derived Cells in a Model of Multiple Sclerosis
Ronald Coleman, The Scripps Research Institute
8:55 PS-10 Use of the Maize Morphogenic Genes BBM and WUS2 to Improve Monocot Transformation
Keith Lowe, Pioneer Hi-Bred International
9:20 PS-11 Transcriptional Signaling in Plant Stem Cell Niches
Yun Zhou, California Institute of Technology
9:50 Discussion

10:00 am – 10:30 am Coffee Break Nautilus


Conveners: Todd Jones, Dupont Pioneer, and Qiudeng Que, Syngenta Biotechnology, Inc.

10:30 am – 12:30 pm Plant Symposium Harbor Island 3

Epigenetics refers to traits that stably inherited, yet potentially reversible, and are the result of physical changes to a chromosome that are not changes in the primary DNA sequence of a gene. Epigenetic traits can arise during normal development or they can be a consequence of an environmental cue. Epigenetic modification of a chromosome can be the result of several different mechanisms, such as DNA methylation and histone modification, but the end result is a change in the accessibility of gene to regulatory molecules. In essence, the epigenome represents a “second code” of genetic information that is responsible for which genes within the genome are expressed or not. This session will provide a perspective of epigenetics, the importance of epigenetics for plant development and epigenetic change as a result of environmental stresses, including the plant tissue culture environment.

10:30 Introduction (T. Jones and Q. Que)
10:35 P-23 Epigenetic Mechanisms for Seed and Fiber Development in Plant Hybrids and Polyploids
Z. Jeffrey Chen, The University of Texas at Austin
11:10 P-24 Epigenetic Regulation of Gene Expression in Maize
Karen McGinnis, Florida State University
11:45 P-25 Epigenome Reprogramming During Tissue Culture and upon Clonal Propagation
Robert Schmitz, The University of Georgia
12:20 Discussion


Moderator:     Brad Upham, Michigan State University and John Harbell, JHarbell Consulting

10:30 am – 12:30 pm Animal Symposium Marina 6

Many factors are driving the study of human disease and associated development of therapeutic interventions from surrogate in vivo models to human cell-based models in vitro. The identification of human-specific molecules or perturbations of normal molecular processes associated with the disease state, provide both a basic understanding of the disease and the prospect of developing therapeutics (both small molecule and/or biologics). Thus, a human test-system becomes essential. Some crucial questions can be addressed with a two dimensional culture format. The National Cancer Institute Experimental Therapeutics Program, which is screening for antineoplastic agents, and the NTP-NIH-EPA High Throughput Screening program, which is targeting the key pathways, molecular events, or processes linked to disease or injury, are two such examples. However, many normal and disease model systems require a more complete assessment of cell differentiation that can only be achieved and maintained through cell to matrix, cell to cell (epithelial) and/or epithelial to stromal cell interactions in three dimensional (3D) culture systems. Traditionally, organ cultures, taken from existing tissues, were employed and they are still the primary choice for tissues that cannot be produced from component cells in vitro (i.e., liver). With the development of iPS cells, and the potential for constructing tissues, de novo, opens possibilities never before possible. This symposium will focus on the development and use of 3D culture models including both reconstructed tissue and organ cultures. It will address the importance of proper matrix selection and structural design, induction and maintenance of differentiated phenotypes, selectively interrogating the 3D culture cell type(s) of interest.

10:30 Introduction (B. Upham and J. Harbell)
10:35  A-17 Prediction of Human Drug-Induced Organ Injury
Alison Vickers, University of California Irvine
11:10 A-18 Decellularized Extracellular Matrix Based Scaffolds for Regenerative Medicine
Karen L. Christman, University of California San Diego
11:45 A-19 3D Bioprinting for Functional In Vitro Tissue Models
Shaochen Chen, University of California San Diego
12:20 Discussion


Moderator: Michael K. Dame, University of Michigan Medical School, and Durga Attili, University of Michigan Medical School

1:30 pm – 2:30 pm Animal Interactive Poster Session Nautilus
A-2000 Novel Usage of the Natural Phytochemical 3,3-diindolylmethane as an Anticancer Therapeutic and Preventative Agent for Melanoma
Jessica Baral, University of California and Mission San Jose High School, and Gary Firestone
A-2001 Extending Neuronal Regeneration with Nano-sized Topography and Various Matrix Coatings
Christoper J. Dipollina, Midwestern University, Harsh Sharthiya, Darryl Giambalvo, and Michele Fornaro
A-2002 A Novel Squash Bug Cell Line
Cynthia L. Goodman, USDA-ARS-BCIRL, Yaofa Li, Kaile Zhou, Joseph Ringbauer, Tamra Lincoln, and David Stanley
A-2003 In-vitro Effect of Electromagnetic Mobile Phones Radiation on Sea Urchin Fertilization Rates
Catalina Perez, Frank W. Cox High School
A-2004 Author is unable to attend. Abstract has been withdrawn.
A-2005 Annexins: A Potential Biomarker For Identifying Tumor Promoting Activity Of Environmental Contaminants
Brad L. Upham, Michigan State University, Iva Sovadinova, Joon-Suk Park, Douglas Whitten, Curtis Wilkerson, and Pavel Babica
A-3010 The NMP1 Inhibitor NSC348884 Affects Oligomerization and Induces Cell Death in Neuroblastoma Cells
Kelly R. Vlcek, Midwestern University, Ryan Kelsch, Nil Akgul, and Kolbrun Kristjansdottir



Moderator:      Sijia Liu, The Samuel Noble Roberts Foundation

1:30 pm – 2:30 pm Plant Interactive Poster Session Nautilus
P-2005 In Vitro Rooting Induction and Expression in Foam-based Matrix Resulted in Improving Performance and Greenhouse Acclimatization In Walnut Cultivars ‘Fernor’ and ‘Chandler’
Laurent Jouve, L&J Biotech, F. Van de Heyning, and V. Rapaka
P-2006 Improved Rooting in Cavendish banana cv. Grande Naine Using Coconut Shell Activated Charcoal and Naphthaleneacetic Acid Supplements
Remedios S. Flamiano, Mindanao State University, Jeanette Madas, Vincent Ryan Flamiano, Rosemarie Diamonon, Lordy Grace Itang, Renan Gubalani, Livilla Donah Ligan, Erna Bautista, and Rebecca Casido
P-2007 Micropropagation of Lycium barbarum (Muragli) of Indian Desert
Smita Shekhawat, University of Delhi, Narpat Shekhawat and Veena Agrawal


Moderators:   Nguyen H. Hoang, University of Florida

1:30 pm – 2:30 pm Plant Interactive Poster Session Nautilus
P-2012 Isolating and Identifying the Proteins Contributing to Nanoparticle Formation in English Ivy (Hedera helix)
Jason Neil Burris, University of Tennessee, Scott Lenaghan, and C. Stewart     
P-2013 Abstract has been withdrawn
P-2014 Micropropagation of Stevia rebaudiana Bertoni in a Temporary Immersion Bioreactor; Phytochemicals Analysis of Plants
Daniela Villamarin, Instituto Politécnico Nacional, Domas Oviedo, Gabriela Sepúlveda, Silvia Evangelista, Jorge Molina, and Mario Rodriquez
P-2015 Establishment of In Vitro Culture of Bougainvillea glabra Choise Variety Sorpresa Mexicana
Christian Marely Rodriquez-Salazar, Instituto Politécnico Nacional, CEPROBI, José Román Reynosa, Silvia Evangelista Lozano, and Antonio Ruperto Jiménez Aparicio
P-2016 Stacking of Two Intragenic Herbicide Resistance Genes Into Sugarcane for Improved Weed Control
Tallyta Nayara Silva, University of Florida, Tufan Oz, and Fredy Altpeter
2:30 pm – 3:30 pm Odd Poster Authors will be present Nautilus


Conveners:     Michael Dame, University of Michigan Medical School, Addy Alt-Holland, Tufts University, and Patrick McNutt, USAMRICD

3:30 pm – 5:00 pm Animal Symposium Marina 6

The last five years has seen a revolution in 3D culture with the advent of self-renewing complex multicellular models derived from a wide range of organ types. These cultures recapitulate tissue-specific architecture and the cell lineage hierarchy, from stem cells to fully differentiated end-stage cell types. This session will explore some of the many exciting prospects that have opened with this new technology such as unparalleled modeling of molecular events, autologous transplantation, co-culture with infectious agents, and preclinical human platforms.

 3:30 Introduction (M. Dame and A. Alt-Holland)
 3:35 A-20 Genomic Characterization and Isolation of LGR5+ Stem Cells from Human Primary Colonic Organoids
Justin A. Colacino, University of Michigan School of Public Health
 4:15 A-21 Primary and Stem Cell-derived 3D Organotypic Co-cultures for Drug Safety, Efficacy and Disease Modeling
Dawn Applegate, RegeneMed
 4:55 Discussion


Moderator:     Piero Barone, Dow AgroSciences LLC, and Monica Schmidt, University of Arizona

3:30 pm – 5:00 pm Plant Symposium Harbor Island 2

The Food and Nutrition Board of the National Academy of Sciences describes a functional food as,“ any modified food or food ingredient that may provide a health benefit beyond that of the traditional nutrients it contains”. Conventional breeding has been successfully applied to increase and/or modify the nutritional value of different crops but in some cases the natural variation of the bioactive component(s) is not enough. Fifteen years ago the development of the pro-vitamin A–enriched ‘Golden Rice represented the first example of genetically engineered food with improved health and nutrition benefits. Since then additional nutritional enhancements in staple crops have been achieved including the modification of fatty acid profile (e.g. omega 3, oleic acid), increased trace mineral(s) bioavailability (e.g. iron, zinc), reduction of anti-nutritional substances (e.g. phytic acid, cyanogens, mycotoxins in corn) and allergens (e.g. P34, Arh2) etc.. As new biotechnology tools become available (e.g. engineered nucleases) and the biochemical basis of traditional food items are being investigated the potential of genetically engineered functional foods moves closer to reality. Such products will become an important tool in solving the world’s malnutrition problem. Also consumers that desire to have a greater role in their overall health and wellness will continue to demand functional foods as part of their balanced diets.

3:30 Introduction (P. Barone and M. Schmidt)
 3:35 P-29 USAID – Creating Functional Foods for Low Resource Farmers
Joseph E. Huesing, United States Agency for International Development
 4:00 P-30 Functional Foods — Making Food/Feed Better for Consumers through Biotechnology
Monica Schmidt, University of Arizona
4:25  P-31 Genomic Studies on Carotenoid Biosynthetic Pathway Genes for Enhancing Selection for ProVitaminA and Total Carotenoids in Maize Grain
Tobert R. Rocheford, Purdue University
4:50 Discussion


Moderator:      Hong Luo, Clemson University

3:30 pm – 5:00 pm Plant Contributed Paper Session Harbor Island 3
3:30  P-1016 Iron and Zinc Biofortification of Cassava Storage Roots to Nutritionally Significant Levels
Nigel J. Taylor, Donald Danforth Plant Science Center, Narayanan Narayanan, Getu Beyene, Raj Deepika Chauhan, Eliana Gaitan Solis, Dimuth Siritunga, Michael Grusak, and Paul Anderson
3:45 P-1017 Passage Through Somatic Embryogenesis Causes Loss of Resistance to Cassava Mosaic Disease in Regenerated Plants
Nigel J. Taylor, Donald Danforth Plant Science Center, Getu Beyene, Raj Deepika Chauhan, Henry Wagaba, John Odipio, Theodore Moll, Titus Alicai, Douglas Miano, Mark Wilson, Hiafeng Weng, Noah Fahlgren, Steve Jacobsen, James Carrington, and Rebecca Bart
4:00 P-1018 Application of Somatic Embryogenesis to Hybrid Hemlocks for Use in Restoration Programs
Scott Merkle, University of Georgia, and Changho Ahn
4:15 P-1019 Identification and Evaluation of Root Specific Promoters for Efficient Gene Expression in Citrus
Manjul Dutt, University of Florida, and Jude Grosser
4:30 P-1020 This presentation has been moved to Monday
4:45 P-1021 Morphoregulatory Role of Melatonin and Serotonin: Implications in Plant Conservation In Vitro
Praveen K. Saxena, University of Guelph

5:00 pm – 5:45 pm SIVB Business Meeting
(All Members Are Urged to Attend)
Student Award Presentations
Executive Conference Room 2A & 2B
5:45 pm – 9:00 pm An Evening Cruise on the San Diego Bay
Admittance by Advance Ticket Holders Only
San Diego Bay



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