Sunday – June 12, 2016

For your viewing convenience, the 2016 World Congress on In Vitro Biology Final Program has been broken down by day.

Daily Program
Abstracts & Posters
Keynote Speaker Index Plant Symposia & Workshops
Saturday, June 11 Plenary Symposia Plant Contributed Papers
Sunday, June 12 Keynote Symposium Plant Posters
Monday, June 13 Animal Posters Education Posters
Tuesday, June 14 Joint Symposia Animal & Education Contributed Papers
Wednesday, June 15 Animal Symposia & Workshops Addendum Booklet
International Conference Symposia

 



Daily Program-at-a-Glance

Time Event Location
7:00 am – 5:30 pm Registration Bayview Foyer
10:00 am – 2:00 pm and 5:30 pm – 7:30 pm Exhibits and Posters Nautilus
Morning 7:00 am – 12:30 pm
7:00 am – 9:00 am In Vitro Animal Cell Sciences Program Committee Meeting Marina 6
Plant Biotechnology Program Committee Meeting Harbor Island 1
8:00 am – 10:00 am Cannabis sativa: The Science Behind the Smoke Harbor Island 2
10:00 am – 10:30 pm Coffee Break Nautilus
Nominating Committee Meeting Marina 4
Public Policy Committee Meeting Executive Center 2A
10:30 am – 12:30 pm Automation in Tissue Culture Harbor Island 2
Genome Editing Harbor Island 3
Post Translational Modification of Proteins Marina 6
11:00 am – 12:00 pm SIVB/IAPB/Springer Business Meeting Marina 4
Afternoon 12:30 pm – 6:30 pm
12:30 pm – 1:30 pm Membership Committee Meeting Executive Center 2A
In Vitro – Plant Editorial Board Meeting Marina 4
Exhibitors/SIVB Refreshment Break Nautilus
1:30 pm – 3:00 pm Biotechnology for Woody Plants Harbor Island 3
Innovative In Vitro Approaches to Improve Plant Germplasm Storage, Production and Conservation Harbor Island 2
Joyful Tissue Culture for Cancer and Aging – Intervention of Stress and Disease Marina 6
3:10 pm – 5:30 pm 2016 World Congress on In Vitro Biology Opening Ceremony Harbor Island 2
3:10 Welcome and Opening Remarks
 3:20 Robert H. Lawrence, Jr. Keynote Symposium
 4:10 Questions and Answers
 4:25 2016 Society for In Vitro Biology Awards Ceremony
4:30 70th Anniversary of Society for In Vitro Biology Presentation
 4:40 Distinguished and Young Scientist Award Recipients
(Awards to be presented at Section Meetings)
4:45 Distinguished Service Award Presentations
5:00 Lifetime Achievement Award Presentations
5:30 pm – 6:30 pm Opening Ceremony Reception Nautilus
Evening 6:30 pm – 9:30 pm
6:30 pm – 7:30 pm Poster Session
Odd Poster Authors will be present
Nautilus
7:30 pm – 9:30 pm Student Symposium: Science in Short: Communicating Your Work Clearly and Effectively Harbor Island 1

Sunday, June 12

7:00 am – 5:30 pm Registration Bayview Foyer


CANNABIS SATIVA: THE SCIENCE BEHIND THE SMOKE

Conveners:     Mary Welter, Brad Upham, Michigan State University, and Sylvia Mitchell, University of the West Indies

8:00 am – 10:00 am Plenary Symposium Harbor Island 2

The use of marijuana (Cannabis sativa) for medical purposes dates back thousands of years (2737 BC) when the emperor of China touted the use of Cannabis tea for the treatment of gout rheumatism and malaria. Recent reviews, studies and reports on the medicinal use of marijuana indicates numerous conditions or diseases (MS, neuropathy, chronic pain, epilepsy, bipolar conditions, Alzheimer’s, digestive disorders, autism, arthritis, nausea, concussions, etc.) that marijuana can be used for as a treatment option. In 1937 the Marijuana Tax Act imposed such high taxes on doctors, pharmacists and growers that the use of marijuana fell out of favor as a medicinal drug in the United States. Because the FDA has classified it as a Schedule 1 drug (high potential for abuse and no legitimate therapeutic uses) it has been difficult to do legitimate studies on the medicinal uses of marijuana in the US. However recent changes by the federal government that eliminated the need for submission of research proposal to the Public Health Services (FDA is still required) has shown that the Federal government is now opening up to reducing barriers that impede marijuana research studies. As of 2015, it was legal to dispense marijuana for medicinal treatments in 23 US states and in the District of Columbia. As public acceptance has dramatically increased for marijuana medical use, the need for legitimate research studies (medicinal effects, micropropagation and breeding) has increased. This session will examine the political and legal aspects of medical marijuana, an international perspective, a review of micropropagation and germ plasm preservation, and potential adverse health effects of chronic, long term use of marijuana.

8:00 Introduction (M. Welter)
8:05 PS-1 Local, National and International Perspectives of a Tenured Cannabis Advocate
Kayvan S. T. Khalatbari, Denver Relief Consulting
8:30 PS-2 Potential Long-term Adverse Health Effects of Chronic Exposures to Compounds Relevant to Marijuana Smoke
Brad L. Upham, Michigan State University
8:55 PS-3 Global Perspectives on the Biotechnology and Tissue Culture Propagation of Cannabis
Sylvia A. Mitchell, University of the West Indies
9:20 PS-4 An Overview of In Vitro Propagation of Elite Cannabis sativa L.
Hemant Lata, University of Mississippi
9:45 Discussion



10:00 am – 10:30 am Coffee Break Nautilus


AUTOMATION IN TISSUE CULTURE

Convener:       Sukhpreet Sandhu, Bayer CropScience, and David Songstad, Cibus LLC

10:30 am – 12:30 pm Plant Symposium Harbor Island 2

Tissue and cell culture involves labor intensive repetitive steps requiring absolute sterility and controlled environment conditions. Automation of some or all steps in the process is considered in the industry to reduce ergonomic injury, decrease labor costs and improve consistency. Animal cell culture platforms have been developed that can operate with minimum technical oversight for a period of days or weeks and allow for evaluation of pH, nutrient or waste concentration, as well as cell concentration and viability. These systems work mostly as fed-batch cultures in which spent media containing metabolic waste products are removed and fresh media is periodically added. Commercial companies have exploited automation in plant tissue culture for media preparation, automated environmental control, in vitro evaluation and robotic planting. However, automation in plant tissue culture is far from achieving its full potential. This session will discuss recent advances in tissue culture automation, and bring forth challenges and opportunities to create a benchmark for automation in tissue culture and plant production.

10:30 Introduction (S. Sandhu and D. Songstad)
10:35 P-1 Construction of a Yeast Artificial Chromosome Containing a Yeast-codon-optimized Brazzein Gene and Its Expression in Saccharomyces cerevisiae
Stephen R. Hughes, USDA, ARS, NCAUR, BBC
11:10 P-2 Development and Use of an Automated Image Capture System for Semi-continuous Monitoring of Promoter-mediated Gene Expression
John Finer, The University of Ohio
11:45 P-3 Evolution of an Automated Synthetic Biology Portal
Steven B. Reidmuller, Synthetic Genomics
12:20 Discussion

 


GENOME EDITING

Convener:       Nancy Reichert, Mississippi State University

10:30 am – 12:30 pm Plant Symposium Harbor Island 3

Plants are intertwined in our lives as we count on them to provide food, feed, shelter, energy, medicines, etc. Since their domestication, an overarching goal has been to improve plants to better-fit our increasing needs. Twentieth century efforts began with use of mutagens that produced imprecise genetic changes (known and unknown); the advent of genetic engineering enabled biologists to deliver genes into plants, although technologies mostly lacked the ability to target specific locations within genomes. Advanced technologies have refined our ability to modify plant genomes with increased precision. Three of these vibrant technologies will be highlighted and described in this session. They include use of the Rapid Trait Development System (RTDS; oligonucleotide-directed mutagenesis), transcription activator-like effector nucleases (TALEN), and the clustered regularly interspersed short palindromic repeats-CRISPR-associated proteins (CRISPR-Cas) system. These technologies share the ability for precise targeting and editing in plant genomes, adaptability for use in numerous plant species, and the promise of future editing possibilities.

10:30 Introduction
10:35  P-4 Precision Genome Editing Tools for Non-transgenic Trait Development
Greg Gocal, Cibus US LLC
11:10 P-5 Precision Genome Editing with Transcription Activator-like Effector Nucleases
Nicholas Baltes, Calyxt, Inc.
11:45 P-39 TBD
Wayne Parrott, University of Georgia
12:20 Discussion


POST TRANSLATIONAL MODIFICATION OF PROTEINS

Conveners: Kolla Kristjansdottir, Midwestern University, and Joshua Gasiorowski, Midwestern University

10:30 am – 12:30 pm Animal Symposium Marina 6

Post-translational modifications of a protein can dramatically affect its structure, function and stability. While numerous types of modifications have been identified, protein phosphorylation has been one of the more extensively studied, largely because a third of the human proteome is thought to be phosphorylated. Mass spectrometry has allowed for identification of thousands of phosphorylation sites and phosphorylation is a key component of many critical signaling pathways in the cell. The challenge now is to understand the biological role of each phosphorylation site. This session will focus on protein phosphorylation and how it can affect important translational cellular attributes, such as drug sensitivity. We will also learn how novel fluorescent biosensors can be used as a read-out for protein phosphorylation in living cells.

10:30 Introduction (K. Kristjansdottir and J. Gasiorowski)
10:35 A-1 Impact of Post Translational Modifications on Heat Shock Protein-90 Drug Sensitivity
Mehdi Mollapour, SUNY Upstate Medical University
11:10 A-2 Oscillatory Control of Subcellular Calcineurin Activity
Sohum Mahta, University of California San Diego
11:45 A-3 Modulating NPM1 Protein in Neuroblastoma Cells
Kolla Kristjansdottir, Midwestern University
12:20 Discussion

 

BIOTECHNOLOGY FOR WOODY PLANTS

Conveners: Ahmad Omar, University of Florida, and Jayasankar Subramanian, University of Guelph

1:30 pm – 3:00 pm Plant Symposium Harbor Island 3

An increasing world population and rise in demand for woody plants products, has increased the need to produce more product to support the world population. Conventional breeding methods have demonstrated limitations with respect to woody plant improvement due to some of the biological characteristics of woody plants such as nucellar polyembryony, high heterozygosity, long juvenile period, and auto incompatibility. The development of biotechnological tools such as, somaclonal variation, protoplast regeneration and somatic hybridization, genetic transformation and molecular genetics which all required a tissue culture protocol has made it possible to overcome some of these problems. In this session, we will discuss some of state-of-the-art achievement and progress made to integrate the biotechnology tools to the propagation of the woody plants. A number of questions, issues and problems in the application of biotechnology to the woody plants will also be discussed.

1:30 Introduction (A. Omar and J. Subramanian)
1:35 P-7 Mapping Human Taste Perception on the Apple Genome
Daryl Somers,
Vineland Research and Innovation Center
2:00 P-8 PAL Is Not Our Pal; Inhibiting Phenylpropanoid Biosynthesis to Facilitate Protoplast Technologies and Improve In Vitro Growth
Maxwell Jones, University of Guelph
2:25 P-9 Intersections of Plant Biotechnology and Fruit Tree Breeding
Ralph Scorza, USDA-ARS
2:50 Discussion

 


INNOVATIVE IN VITRO APPROACHES TO IMPROVE PLANT GERMPLASM STORAGE, PRODUCTION AND CONSERVATION

Conveners: Maria Jenderek, USDA-ARS, and Michael E.  Kane, University of Florida

1:30 pm – 3:00 pm Plant Symposium Harbor Island 2

The basic principles of tissue culture were established over 130 years ago. During this time, applications of in vitro culture have resulted in significant advances in medicine, drug discovery and animal and plants sciences. In the plant sciences, in vitro techniques have promoted new cultivar development and accelerated pathogen-free micro-propagation and propagation of plant material used in agriculture and landscape architecture. Frequently, in vitro cultures are the primary plant form used in cryopreservation and conservation of endangered plant species. Regardless of the application, truly innovative approaches are necessary to simplify technologies, make them more efficient or extend them to new species. During this session, examples of innovative applications of in vitro plant systems for germplasm storage, production, and conservation will be presented and discussed.

1:30 Introduction (M. Jenderek and M. E. Kane)
1:35 P-10 The Use of Micro-shoots Tip Culture to Eliminate Virus for the Clean Plant Programs at Foundation Plant Services, University of California at Davis
Deborah Golino, University of California at Davis
2:00 P-11 Microbial Contaminants in Tissue Culture: Innovative Techniques for Identification, Management and/or Eradication of Non-pathogenic Endophytic Bacteria in Plant Tissue Culture
Carolyn Sluis, Tissue-Grown Corporation
2:25 P-12 Novel In Vitro Approaches for Orchid Conservation: The Ghost Orchid Case Study
Nguyen H. Hoang, University of Florida
2:40 P-13 Antioxidants Improve Micropropagation and Cryopreservation
Maria Jenderek, NLGRP, USDA-ARS
2:50 Discussion


JOYFUL TISSUE CULTURE FOR CANCER AND AGING – INTERVENTION OF STRESS AND DISEASE
SPONSOR: THE JAPANESE TISSUE CULTURE ASSOCIATION

Convener and Chairperson: Renu Wadhwa, National Institute of Advanced Industrial Science & Technology: AIST

Chairperson: Yukio Nakamura, RIKEN BioResource Center: RIKEN BRC

1:30 pm – 3:00 pm Animal Symposium Marina 6

Tissue culture technologies, in last 3 decades, have walked from simple adherent and floating cultures to multi-dimensional interfaces that have not only been useful for dissecting out complex cell signaling networks, but also have revolutionized regenerative medicine. Tissue culture research has made remarkable contribution to the basic understanding of human cell biology in a variety of normal and abnormal physiological conditions including stress and a variety of diseases. It continues to contribute, in a large way, to preventive and therapeutic aspects of the current scenario of rapidly increasing aging populations, environmental stresses and complexity of a variety of diseases; the major challenges in research and medicine. The present session is planned to cover some significant contributions of tissue culture based research to the basic understanding and interventional biology of stress, aging, cancer and neurodegeneration. Ashwagandha, is a popular herb in Indian traditional home medicine, Ayurveda, that has gathered increasing recognition in recent years. Integrated use of tissue culture, bioinformatics and molecular biology plate-forms for revealing the scientific understanding of medicinal value of Ashwagandha and its active principles for anticancer and neuroregenerative activities will also be presented.

1:30 A-4 Mitochondrial Stress Chaperone Mortalin Contributes to EMT and Cancer Metastasis
Renu Wadhwa, AIST and Tsukuba University
1:50 A-5 PINK1 Regulation of Mitochondrial Homeostasis and Cell Survival
Hitoshi Murata, Okayama University
2:10 A-6 Regulation of Cell Death and Cell Specific Functions by Inhibition of DNA Methylation
Kazuaki Nakamura, National Research Institute for Child Health and Development
2:30 A-7 Anticancer and Antiaging Activities in Ashwagandha Leaves: Endorsement and Mechanisms from Tissue Culture
Sunil Kaul, AIST
2:50 A-8 Cytotoxicity of Ashwagandha Leaf Extract for Telomerase Plus and Minus Cancer Cells – Understanding from Tissue Culture Based Assays
Yue Yu, Tsukuba University


2016 WORLD CONGRESS ON IN VITRO BIOLOGY OPENING CEREMONY

Program Chair: Harold N. Trick, Kansas State University

3:10 pm – 5:30 pm Opening Ceremony Harbor Island 2
3:10 Welcome and Opening Remarks:
Dwight T. Tomes, President, Society for In Vitro Biology
3:20 ROBERT H. LAWRENCE, JR. KEYNOTE SYMPOSIUM
Introduction (B. L. Upham)
KS-1 Seeing Single Molecules, from Early Spectroscopy in Solids, to Super-resolution Microscopy, to 3D Dynamics of Biomolecules in Cells
William E. Moerner, Nobel Prize Winner in Chemistry (2014), Harry S. Mosher Professor of Chemistry and Professor, by courtesy, of Applied Physics
4:10 Questions and Answers
4:25 2016 SOCIETY FOR IN VITRO BIOLOGY AWARDS CEREMONY
Dwight T. Tomes, President, Society for In Vitro Biology
4:30 70th Anniversary of Society for In Vitro Biology Presentation
Delia R. Bethell
4:40 2016 Distinguished and Young Scientist Award Recipients (Awards to be presented at Section Meetings)

Distinguished Scientist: Guy Smagghe, Ghent University
Young Scientist: Joshua Z. Gasiorowski, Midwestern University
4:45 2016 Distinguished Service Award Presentation
(Awards to be presented by Eugene Elmore):
Barbara B. Doonan, New York Medical College
Barbara M. Reed
Elizabeth J. Roemer, SUNY Stony Brook
Nancy Reichert, Mississippi State University
David D. Songstad, Cibus LLC
Mary Welter
Allan Wenck, Bayer CropScience
5:00 2016 Lifetime Achievement Award Presentations:
(Introduction by John W. Harbell; Acknowledgement by Dr. Elmore to follow)
Eugene Elmore
5:15 (Introduction by Sandra L. Schneider; Acknowledgement by Dr. Reid to follow)
Yvonne A. Reid, ATCC
5:30 Adjourn
Group photo with Dr. Moerner and Student attendees

5:30 pm – 6:30 pm 2016 WORLD CONGRESS ON
IN VITRO BIOLOGY
OPENING CEREMONY RECEPTION
Nautilus
5:30 pm – 7:30 pm Exhibits and Posters Nautilus
6:30 pm – 7:30 pm Odd Poster Authors will be Present Nautilus
 

STUDENT SYMPOSIUM: SCIENCE IN SHORT: COMMUNICATING YOUR WORK CLEARLY AND EFFECTIVELY

Conveners: Jordan Brungardt, Kansas State University, and Matt Desrosiers, Worcester Polytechnic Institute

7:30 pm – 9:30 pm Education Symposium Harbor Island 1

The ability to cater to a particular person or audience to efficiently convey information is an essential part of any student’s repertoire.  Proficiency in communication means the difference between leaving an audience confused or captivated.  Everyday encounters bring students into contact with a diversity of people that requires students to think about their research in new ways.  This symposium will exercise the participant’s ability to quickly size up an audience and tailor a narrative that is appropriate for the given audience.  Students will be given a short amount of time to talk about their research with persons of varying backgrounds, including leaders in the field and the scientifically illiterate.  Participants will rate the conversation so that the student can evaluate the effectiveness of their communicating style.

7:30 Introduction (J. Brungardt and M Desrosiers)
7:35 Wayne Parrott, University of Georgia
8:00 Panel Discussion
Panelists:
Mae Ciancio, Midwestern University
Michael K. Dame, University of Michigan Medical School
Jessica L. Rupp, Montana State University
Dwight T. Tomes
Brad L. Upham, Michigan State University
Pamela J. Weathers, Worcester Polytechnic Institute
Allan Wenck, Bayer CropScience

 

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