Saturday – June 11, 2016

For your viewing convenience, the 2016 World Congress on In Vitro Biology Final Program has been broken down by day.

Daily Program
Abstracts & Posters
Keynote Speaker Index Plant Symposia & Workshops
Saturday, June 11 Plenary Symposia Plant Contributed Papers
Sunday, June 12 Keynote Symposium Plant Posters
Monday, June 13 Animal Posters Education Posters
Tuesday, June 14 Joint Symposia Animal & Education Contributed Papers
Wednesday, June 15 Animal Symposia & Workshops Addendum Booklet
International Conference Symposia


Daily Program-at-a-Glance

Time Event Location
7:00 am – 7:00 pm Registration Bayview Foyer
7:00 pm – 9:00 pm Exhibits and Posters Nautilus
Morning 7:00 am – 12:30 pm
8:00 am – 12:15 pm SIVB Board of Directors Meeting Executive Center Boardroom
9:00 am – 5:00 pm Plant Tissue Culture Workshop for High School and Community College Teachers
Registration by advance purchase only
Marina 3
9:15 am – 5:30 pm 14th International Conference on Invertebrate and Fish Cell Culture
9:15 am – 12:00 pm International Conference:
Establishing Insect Cell Lines for Basic and Applied Biology
12:00 pm – 1:45 pm Lunch on your own
Afternoon 12:30 pm – 6:30 pm
1:45 pm – 4:30 pm International Conference:
Beyond Fifty Years of Research with Fish Cell Lines
3:00 pm – 6:00 pm Poster Set up Nautilus
3:30 pm – 4:30 pm 2016 Program Planning Committee Meeting Marina 4
4:30 pm – 5:30 pm International Conference:
Roundtable Discussion on Conference Topics
5:00 pm – 6:00 pm Finance Committee Meeting Marina 1
Evening 6:30 pm – 9:30 pm
7:00 pm – 9:00 pm 2016 World Congress in In Vitro Biology Welcome Reception Nautilus
7:00 pm – 9:00 pm America’s Finest Silent Auction Kickoff Nautilus
7:30 pm – 8:30 pm Poster Session
Even Posters Authors will be present

Saturday, June 11

7:00 am – 7:00 pm Registration Bayview Foyer
8:00 am – 12:15 pm SIVB BOARD OF DIRECTORS MEETING Executive Center Boardroom

9:00 am – 5:00 pm Marina 3

Instructors: Carol Stiff, Kitchen Culture Kits, Inc., Kim Hanson, Cibus, LLC, and Sylvia Mitchell, University of the West Indies

This is a special hands-on training program specifically designed for Community College and High School instructors/teachers of Biotechnology Programs. Learn how to teach the basics of plant cell tissue culture from Carol Stiff of Kitchen Culture Kits. This all-day event will provide you with the skills you need to teach tissue culturing to your students. A laboratory guide and additional resources will be provided so that instructors will be able to set up appropriate course work when they return to their institutions. Advance registration is required to participate in this event.


Welcome & Introduction to Trainers and Assistants (Resource & Local Experts). Preparation for Plant Cell/Tissue Culture Work: Safety, MSDS, Equipment


Media Preparation (Kitchen vs. Lab Methods), Assemble Sterile “Hoods” and Prep Areas, Concepts of Sterile Technique

12:00 Lunch

Presentation on Plant Tissue Culture for Teachers & Budding Enthusiasts


Demo of Disinfection and Culture of Dicot, Monocot, Tissue & Cell Suspensions


Hands-on Disinfection and Culture of Crop Plants, Sub-culture of Established Culture


Discussion: Problems/Issues, Trouble Shooting, Additional Resources, Clean-up

 5:00 Concluding remarks


Co-Chairs: Lucy E. J. Lee, University of the Fraser Valley, Cynthia L. Goodman, USDA, ARS, BCIRL, and David Stanley, USDA, ARS, BCIRL

Over more than half a century after its first gathering in 1962, when it started as an International Colloquium on Invertebrate Tissue Culture in Montpellier, France, this forum held every 4 years at various international venues, evolved to include aquatic invertebrates and fish cell cultures. Now in it’s 14th iteration, the 2016 meeting of the International Conference on Invertebrate and Fish Cell Culture promises to bring top specialists in biomedical and agricultural fields to provide us with recent advances and applications of fish and invertebrate in vitro work in their respective areas of research.


Conveners:     Cynthia L. Goodman, USDA, ARS, BCIRL, and David Stanley, USDA, ARS, BCIRL

9:15 am – 12:00 pm Conference Symposium Seabreeze

Established insect cell lines have a long history as platforms supporting industrial and research operations. They can be engineered for use in high-throughput screening processes and for producing recombinant proteins of biomedical value. They are important instruments in research into cell-virus interactions, such as viral resistance mechanisms and host range studies. Cell lines established from a variety of insect species within a group are used to investigate host ranges of insect viruses, including baculoviruses. They also serve in studies of basic insect biology. It would be difficult to overestimate the present and growing future value of insect cell lines. In this symposium we highlight four emerging areas of insect cell lines. The first paper will clarify increasing industrial needs for new, tissue-specific insect cell lines used to reduce discovery costs. Gene-silencing constructs are readily incorporated into established cell lines, facilitating basic research into gene identification and functions, the point of our second presentation. The bacterial genus, Wolbachia (named after its co-discoverer, Burt Wolbach), probably infects most insect species, particularly in testes and ovaries. Some species are unable to reproduce without Wolbachia infection. Our third talk will address important work on Wolbachia interactions with established insect cell lines, helping understand mechanisms of male killing, feminization and cytoplasmic incompatibility. Finally, our last contribution addresses primary insect hemocyte cultures, now providing insight into signal mechanisms that drive cellular immune actions, including cell spreading, hemocyte migration and phagocytosis. These four presentations highlight the promise and excitement of establishing and using insect cell lines in a growing spectrum of industrial and academic research and discovery programs.

9:15 Introduction (C. L. Goodman and D. Stanley)
9:30 I-1 Applications of Insect Cell Lines in Industry
Jessica Monserrate, Bayer CropScience
10:00 I-2 RNAi in Insects: Use of Insect Cell Cultures to Better Understand and Increase Efficacy
Guy Smagghe,
Ghent University
10:30 I-3 Manipulating Wolbachia Infection and Maintenance in Mosquito Cell Lines
Ann Fallon, University of Minnesota
11:00 I-4 Prostaglandin Actions in Insect Immunity: Results from Primary Hemocyte Cultures
David Stanley, USDA, ARS, BCIRL
11:30 Discussion

12:00 pm – 1:45 pm Lunch on your own


Convener:              Lucy E. J. Lee, University of the Fraser Valley

1:45 pm – 4:30 pm Conference Symposium Seabreeze

Cell cultures have been excellent investigative models that have advanced the sciences in many areas of applied biology and medicine, especially since the availability of continuous cell cultures or cell lines. Fish cell cultures have also been instrumental in the advancement of basic sciences, and since 1962 when the seminal paper by Wolf and Quimby was published in Science describing the first fish cell line, fish cell lines have had major impacts not only in basic biology, but also in fisheries and aquaculture, environmental toxicology, as well as in various areas of comparative virology, immunology, parasitology, endocrinology, etc.   This session provides a glimpse into the history of fish cell lines, their availability, characteristics and application.

1:45 Introduction (L. E. J. Lee)
2:00 I-5 Fish Cell Line: Development, Characterization and Conservation for In Vitro Research in India
Mukunda Goswami, ICAR-Central Institute of Fisheries Education, Mumbai
2:45 I-6 Are Cell Lines Truly Immortal If Their Developers Are Mortal?
Niels Bols, University of Waterloo
3:30 I-7 Collagen Producing Fish Cell Lines and Their Use in Biomedical Research
Lucy E. J. Lee, University of the Fraser Valley
4:15 Wrap-up and Discussion
3:30 pm – 4:30 pm 2016 PROGRAM PLANNING COMMITTEE MEETING Marina 4


Moderators: Lucy EJ Lee, University of the Fraser Valley, Cynthia L. Goodman, USDA, ARS, BCIRL, and David Stanley, USDA, ARS, BCIRL

4:30 pm – 5:30 pm Conference Workshop Seabreeze

All speakers from both sessions of the conference will be invited to serve on a panel and field questions from the audience. Discussions will be encouraged between the speakers and audience members on any topics relating to the presentations.


Niels Bols, University of Waterloo
Ann Fallon, University of Minnesota
Mukunda Goswami, Deemed University
Lucy E. J. Lee, University of the Fraser Valley
Guy Smagghe, Ghent University
David Stanley, USDA, ARS, BCIRL


7:00 pm – 9:00 pm WELCOME RECEPTION Nautilus
7:30 pm – 8:30 pm Even Poster Authors will be present Nautilus




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