Monday – June 13, 2016

For your viewing convenience, the 2016 World Congress on In Vitro Biology Final Program has been broken down by day.

Daily Program
Abstracts & Posters
Keynote Speaker Index Plant Symposia & Workshops
Saturday, June 11 Plenary Symposia Plant Contributed Papers
Sunday, June 12 Keynote Symposium Plant Posters
Monday, June 13 Animal Posters Education Posters
Tuesday, June 14 Joint Symposia Animal & Education Contributed Papers
Wednesday, June 15 Animal Symposia & Workshops Addendum Booklet
International Conference Symposia

 



Daily Program-at-a-Glance

Time Event Location
7:00 am – 6:90 pm Registration Bayview Foyer
10:00 am – 3:30 pm Exhibits and Posters Nautilus
Morning 7:00 am – 12:30 pm
7:00 am – 8:00 am Publications Committee Meeting Marina 1
8:00 am – 10:00 am In Vitro Biology: From Tools to Reality Harbor Island 2
10:00 am – 10:30 pm Coffee Break Nautilus
Awards Committee Meeting  Marina 1
10:30 am – 12:30 pm Gene K/O, Micro RNA, RNAi, and Altering Gene Regulation Marina 6
Plant Biotechnology Post Doctoral Oral Presentation Competition Harbor Island 3
Plant Biotechnology Student Oral Presentation Competition Harbor Island 2
Afternoon 12:30 pm – 6:30 pm
12:30 pm – 2:00 pm Student Luncheon: Networking Platform Harbor Island 1
Long Range Planning Committee Meeting Marina 4
Refreshment Break Nautlius
1:30 pm – 2:30 pm In Vitro Animal Cell Sciences Student and Post Doctoral Oral Presentation Competition Marina 6
Interactive Poster Sessions
BiotechnologyGenetic Engineering and Secondary Metabolism
Nautilus
2:30 pm – 3:30 pm Even Poster Authors will be present Nautilus
3:30 pm – 5:00 pm Management of Resistance in GM Plants Harbor Island 2
Maximizing the Power of Animal Cell Factory Marina 6
Non-Competitive Student Oral Presentations Harbor Island 1
Novel Ideas for Product Development in Plants Harbor Island 3
5:00 pm – 6:00 pm The Advent of Precision Breeding: An Advanced Method for Crop Genetic Improvement Harbor Island 3
In Vitro Animal Cell Sciences and Education Contributed Papers Marina 6
Product Applications, Micropropagation and Other In Vitro Plant Topics Harbor Island 2
Evening 6:00 pm – 9:30 pm
6:00 pm – 7:30 pm Plant Biotechnology Section Business Meeting Harbor Island 1
In Vitro Animal Cell Science Section Business Meeting Marina 6
7:30 pm – 10:00 pm Joint Sections’ Social Bayview Lawn

Monday, June 13

7:00 am – 6:00 pm Registration Bayview Foyer

IN VITRO BIOLOGY: FROM TOOLS TO REALITY

Conveners:    Joseph Petolino, Ivy Tech Community College, and Addy Alt-Holland, Tufts University

8:00 am – 10:00 am Plenary Symposium Harbor Island 2

Developments in in vitro biology have played a central role in the continued march toward a deeper understanding of life processes by providing enhanced capabilities to study cellular and developmental phenomenon and new experimental tools to address fundamental questions.  The application of some of these in vitro methods has had significant social implications.  For example, the deployment of transgenic crops with genetically-based insect and herbicide resistance has been a major source of debate since the first products reached the marketplace in the mid-1990s.  Similarly, breakthroughs in human reproductive development, such as in vitro fertilization and embryonic stem cell culture, have shaped much of the dialog over the last few decades relative to the appropriate use of technology for human applications.  New developments in genome editing will no doubt provide a continued means for generating an even deeper understanding of biological function but will also add an additional dimension to the social dialog.  The reality of experimentally modifying plant and animal genomes introduces additional questions for society to ponder.  This session will examine some of the technical advances that have led to the ability to modify genomes, some of the issues associated with the regulation of genetically modified organisms and the some of the ethical considerations of human germline engineering.

8:00 Introduction (J. Petolino and A. Alt-Holland)
8:05 PS-5 The Elements of Genome Editing Style
Fyodor D. Urnov, Sangamo BioSciences, Inc.
8:40 PS-6 Regulatory Aspects of Genome-Edited Crops
Jeff Wolt, Iowa State University
9:15 PS-7 Editing and the Biosphere’s Genomes: Human Issues for Tomorrow; Non-Human Issues for Today
Henry T. Greely, Stanford University Law School
9:50 Discussion

10:00 am – 10:30 am Coffee Break Nautilus


GENE K/O, MICRO RNA, RNAI, AND ALTERING GENE REGULATION

Conveners:  Michael Fay, Midwestern University, and Joshua Gasiorowski, Midwestern University

10:30 am – 12:30 pm Animal Symposium Marina 6

Eukaryotic genes are rarely regulated by simple on/off molecular switches. Studying these complex regulatory mechanisms, even within controlled cell culture systems, can be a significant challenge without the appropriate tools. RNA interference (RNAi) strategies have been used extensively to generate transcriptional knock-downs that can help elucidate gene utility through transient, loss-of-function experiments. Emerging genome editing technologies, such as CRISPR, have allowed researchers to stably manipulate genomes with a high level of precision and efficiency. Additionally, over-expression of micro RNAs have been employed to alter gene expression during post-transcriptional processing. This session will highlight and summarize some of the current molecular methods that have been successfully used to influence and study gene regulation at the genome, transcriptional, and transcript-processing levels.

10:30 Introduction (M. Fay and J. Gasiorowski)
10:35 A-9 RNAi Therapeutics: Tackling a Billion Year Old Barrier with New siRNN Chemistry
Steven F. Dowdy, University of California,San Diego
11:10 A-10 Modulation of Gene Expression by Polymer Nanocapsule Delivery of DNA Cassettes Encoding Small RNAs
Jing Wen, UCLA
11:45 A-11 TBD
John C. Burnett, City of Hope
12:20 Discussion

 


PLANT BIOTECHNOLOGY POST DOCTORAL ORAL PRESENTATION COMPETITION

Moderator:      Sadanand Dhekney, University of Wyoming

Panel of Plant Biotechnology Experts Evaluating the Contestants: Prakash Kumar, National University of Singapore, Dennis Gray, University of Florida, and Daniel Bergey, University of Wyoming

10:30 am – 12:30 pm Plant Contributed Paper Competition Harbor Island 3

To support the Society’s vision to encourage education and scientific informational exchange and recognize outstanding students, the Plant Biotechnology Section is pleased to announce the 2016 Postdoctoral competition. Finalists will be selected based on the quality of the abstracts. The abstract should address the following: Background, Objectives, Methods, Results, Discussion and Conclusions. Where appropriate, the methods section should include a description of how reproducible results were ensured. The abstract must not include references. The abstract text must not exceed 1800 characters. A panel of judges will evaluate the presentations at the meeting. Criteria for the evaluation include experimental design, data analysis, proper interpretation of the results, originality of the study, technical difficulty, appearance and ability of the post-doctoral candidate to present it. Winners will be presented with a certificate and a cash award at the Plant Biotechnology Section’s Business Meeting.

10:30  P-1000 Phloroglucinol Enhances Clonal Micropropagation of Magnolia macrophylla var. ashei
Rachel Folgado, The Huntington Library, Taylor La Val, Sean Lahmeyer, and Tim Thibault
10:50 P-1001 Production of Three New Grapefruit like Cybrids Confirmed by Plant Mitochondrial Intron, Chloroplast and Nuclear DNA Markers to Improve Citrus Canker Resistance
Ahmad Omar, University of Florida, Mayara Murata, Qibin Yu, Fred Gmitter, Jr., Christine Chase, James Graham, and Jude Grosser
11:10 P-1002 Transgenic Brassica juncea Plants Expressing MsrA1, a Synthetic Cationic Antimicrobial Peptide, Exhibit Resistance to Fungal Phytopathogens
Deepak Kumar, Banasthali University, Anjana Rustagi, Mohd. Aslam Yusuf, and Neera Bhalla Sarin
11:30 P-1003 Host-derived Gene Silencing of Pathogen Fitness Genes Improves Resistance to Soybean Cyst Nematodes in Soybean
Bin Tian, Kansas State University, Jiarui Li, Lila Vodkin, Timothy Todd, John Finer, and Harold Trick
11:50  P-1004 Evaluating Survival in Shoot Tips of Several Species Stored in Liquid Nitrogen for 4 to 16 Years
Anne-Catherine Vanhove, Cincinnati Zoo & Botanical Garden, Megan Philpott and Valerie Pence
12:10 P-1022 Improving Photosynthetic Efficiency in Sugarcane by Expression of Pyruvate Orthophosphate Dikinase from Miscanthus x giganteus
Ratna Karan, University of Florida, Baskaran Kannan, Nikhil Jaikuma,  Kankshita Swaminathan, Liang Xie, Brandon James, Steve Moose, Fredy Altpeter, and Steve Long


PLANT BIOTECHNOLOGY STUDENT ORAL PRESENTATION COMPETITION

Moderator: Cecilia Zapata, Driscoll’s

Panel of Plant Biotechnology Experts Evaluating the Contestants: Michael E. Kane, University of Florida, Randall Niedz, USDA/ARS, and Nancy Reichert, Mississippi State University

10:30 am – 12:30 pm Plant Contributed Paper Competition Harbor Island 2

To support the Society’s vision to encourage education and scientific informational exchange and recognize outstanding students, the Plant Biotechnology Section is pleased to announce the 2016 Student Oral Presentation Competition. Student competition finalists will be selected based on the quality of the abstracts. The abstract should address the following: Background, Objectives, Methods, Results, Discussion and Conclusions. Where appropriate, the methods section should include a description of how reproducible results were ensured. The abstract must not include references. The abstract text must not exceed 1800 characters. A panel of judges will evaluate the presentations at the meeting. Criteria for the evaluation include experimental design, data analysis, proper interpretation of the results, originality of the study, technical difficulty, appearance and ability of the student candidate to present it. Winners will be presented with a certificate and a cash award at the Plant Biotechnology Section’s Business Meeting.

10:30  P-1005 Identification and Validation of Male Specific ISSR and SRAP Molecular Markers in Jojoba [Simmondsia chinensis (Link) Schneider] – An Economically Important Dioecious Crop
Monika Heikrujam, University of Delhi, Jatin Kumar, and Veena Agrawal
10:50 P-1006 Analyzing Somatic Embryogenesis Gene Expression in Response to Tissue Culture Enhancer PLA1 Protein
Tina S. Lai, Penn State University, Katie Lagenski, and Wayne Curtis
11:10 P-1007 Susceptibility and Race Specific Wheat Genes Induced by Six Puccinia triticina Races
Kerri Neugebauer, Kansas State University, John Fellers, and Harold Trick
11:30 P-1008 Improvement of Embryogenic Callus Production from Coconut (Cocos nucifera L.) Plumule Explant
Quang T. Nguyen, The University of Queensland, Irina Antonova, Juan Pablo Romero Naranjo, Elizabeth Aitken, Mike Foale, and Steve Adkins
11:50 P-1009 The Long-term Population Genetics of a Micropropagated Reintroduction of Minuartia cumberlandensis, a Federally Endangered Perennial Plant
Megan Philpott, Cincinnati Zoo & Botanical Garden, and Valerie Pence
12:10 P-1010 Metabolic Engineering of Sugarcane for Hyperaccumulation of Oil in Vegetative Biomass
Saroj Parajuli, University of Florida, Ratna Karan, Georgina Sanahuja, Hui Liu, John Shanklin, and Fredy Altpeter

 


STUDENT LUNCHEON: NETWORKING PLATFORM

Conveners:     Jordan Brungardt, Kansas State University, and Matt Desrosiers, Worcester Polytechnic Institute

12:30 pm – 2:00 pm Education Workshop Harbor Island 1

Networking and interacting with other scientists is a great way to for graduate students and post-docs to learn more about fields related to their own as well as get an outside perspective on their research.  Not only does it have the potential to spark new ideas about one’s current work, but it also provides a way of meeting future employers and collaborators.  This luncheon is designed to help students meet other students as well as long-time members of the SIVB in both similar and different fields so that students can develop professional contacts.  Experts from academia, industry, and government will be in attendance and students are encouraged to exchange ideas with as many people as possible.

12:30 Introduction (J. Brungardt and M. Desrosiers)
12:35 Lunch

 


IN VITRO ANIMAL CELL SCIENCES STUDENT AND POST DOCTORAL ORAL PRESENTATION COMPETITION

Moderators:    Addy Alt-Holland, Tufts University, and Kolla Kristjansdottir, Midwestern University

Panel of In Vitro Animal Cell Sciences Experts Evaluating the Contestants: Addy Alt-Holland, Tufts University, Mae Ciancio, Midwestern University, Barbara Doonan, New York Medical College, Michael J. Fay, Midwestern University, John W. Harbell, JHarbell Consulting, LLC, Kolla Kristjansdottir, Midwestern University, and Brad L. Upham, Michigan State University

1:30 pm – 2:30 pm Animal Contributed Paper Competition Marina 6

To support the Society’s vision to encourage education and scientific informational exchange and recognize outstanding students and post docs, the In Vitro Animal Cell Sciences section is pleased to announce the 2015 In Vitro Animal Cell Science Research Oral Presentation Competitions for both Students and Post Docs. Students and Post Docs wishing to participate in this competition submitted a copy of their title and abstract (as submitted to SIVB) and abstract tracking number to Drs. Addy Alt-Holland and Kristjansdottir. Only those abstracts formally submitted to both SIVB and the moderators were considered. Finalists were selected based on the quality of the abstracts. A panel of judges will evaluate the presentations at the meeting. Criteria are experimental design, data analysis, proper interpretation of the results, originality of the study, technical difficulty, appearance of the presentation and ability of the finalist to respond to questions. Winners will be presented with a certificate and a cash award at the In Vitro Animal Cell Sciences Business Meeting.

1:30  A-1000 Voluntary Exercise Prevents High-fat Diet-induced Obesity: Effect on Intestinal Barrier Integrity
Elizabeth Hansen, Midwestern University, Matt Pytynia, Aneesh Chawla, Mae Ciancio, and Christian Evans          
1:50 A-1001 Extending Neuronal Regeneration with Nano-sized Topography and Various Matrix Coatings
Darryl Giambalvo, Midwestern University, Harsh Sharthiya, Christopher Dipollina, Kolla Kristjansdottir, Joshua Gasiorowski, and Michele Fornaro
2:10 A-1002 Development of a Stepwise Protocol for the Generation of Human Induced Pluripotent Stem Cell-derived Choroidal Endothelial Cells
Allison Songstad, University of Iowa, Luke Wiley, Erin Burnight, Steven Whitmore, Emily Kaalberg, Megan Riker, Edwin Stone, Robert Mullins, and Budd Tucker


BIOTECHNOLOGY

Moderator:      Bhuvan Pathak, University of Arkansas

1:30 pm – 2:30 pm Plant Interactive Poster Session Nautilus
P-2000 In Vitro Conservation of Elite Germplasm, Isolation and Up-regulation of Some Important Biomolecules
Veena Agrawal, University of Delhi
P-2001 Development of an Efficient In Vitro Regeneration System Amenable to Agrobacterium– mediated Transformation of Enset
Jonathan M. Matheka, International Institute of Tropical Agriculture, Jaindra Tripathi, Endale Gebre, and Leena Tripathi
P-2002 Implementation of Precision Breeding for Grapevine Improvement – A Technical Perspective
Zhijian T. Li, University of Florida, and Dennis J. Gray
P-2003 Development of a Robotic Platform for Automated Protoplast Isolation, Transformation, and Screening of Plant Suspension Cultures
Elizabeth M. Dlugosz, University of Tennessee, Taniya Dhillon, Jason Burris, Scott Lenaghan, and C. Neal Stewart, Jr.
P-2004 Use of Low Inoculum/Long Co-culture Treatments Lead to Tremendous Enhancements in Agrobacterium-mediated Transformation of Sunflower
John Finer, The Ohio State University, and Zhifen Zhang


GENETIC ENGINEERING AND SECONDARY METABOLISM

Moderator:      Zhanyuan Zhang, University of Missouri – Columbia

1:30 pm – 2:30 pm Plant Interactive Poster Session Nautilus
P-2008 Engineering Low Cd Rice Through Expression of OXS3-like Gene Fragments
David W. Ow, Chinese Academy of Sciences, and Changhu Wang
P-2009 Robust Genetic Transformation of Sorghum (Sorghum bicolor) Using Green Regenerative Tissue
Srinivas Belide, CSIRO Agriculture, Thomas Vanhercke, James Petrie, and Surinder Singh
P-2010 Development of Substantial Biotechnological Methods for Production of Pharmaceutically Active Biometabolites from the Genus Spilanthes
Rakhi Chaturvedi, Indian Institute of Technology Guwahati, and R. Radhika
P-2011 A Rapid Small-scale Wheat Microspore Culture Technique for Screening Multiple Variables
Patricia L. Polowick, National Research Council Canada, Pankaj Bhowmik, Goska Nowak Jean Enns, and Alison Ferrie

2:30 pm – 3:30 pm Even Poster Authors will be present Nautilus

MANAGEMENT OF RESISTANCE IN GM PLANTS

Convener:       Alan R. Wenck, Bayer CropScience

3:30 pm – 5:00 pm Plant Symposium Harbor Island 2

Farmer’s fields present a rich source of nutrients not only for the crop of interest but also for numerous weed species.  Further, food and fiber intended for human and domesticated animal consumption presents a giant resource for insect pests and disease organisms.  There is a strong evolutionary drive to take advantage of this resource and where there is a solution to the pest problem, there is also a strong pressure for that pest to evolve ways to overcome such solutions.  Such an evolutionary battle has been ongoing even before the dawn of agriculture.  This battle has led to a need for resistance management strategies that seek to prolong the life of any proffered solution.  Organizations such as Crop Life International have established and or support international and country-specific working groups on insecticide resistance management (IRAC), herbicide resistance management (HRAC) and fungicide resistance management to increase awareness and to provide guidelines for resistance management.  The use of genetically modified (GM) crops has added another dimension of complexity to resistance management, but it has also added valuable tools to be used in resistance management strategies.  Interestingly, the US Environmental Protection Agency was the first to take a proactive stance and implement strict and enforceable guidelines for insect resistance management for GM plants.  However, in 2014, all major producers of GM crops conferring insect tolerance agreed to conform with Industry standards for insect resistance management and routine audits of IRM programs.  Additional developments in the past decade include European Union ratification of proactive resistance management requirements for conventional (non-GM) insecticides.  Thus, even where IRM strategies are not required by regulatory statutes, the Industry has committed to their implementation . In general, where such strategies have been required (i.e. refuge requirements for BT crops in the US) large scale problems with resistance have been slow to occur. However, where not required or implemented, failures have been observed (i.e. the emergence of glyphosate tolerant weeds and widespread resistance of Spodoptera frugiperda to Cry1F-based maize products in South America). The GM and non-GM industry ignore resistance management at their peril.  Time lines and costs associated with product development and registration (especially for GM crops) require that any released solution is properly managed in order for it to be sustainable, effective and create a return on investment.  This session will address the need for robust resistance management strategies, the challenges of implementation of such on a global basis and success and failures upon their implementation.

3:30 Introduction (A. Wenck)
3:35 P-14 Emerging Resistance to Bt Crops in Latin America Prompts New Global Implementation Paradigm for Insect Resistance Management
Timothy Dennehy, Bayer CropScience
4:00 P-15 Weed Resistance in Herbicide-resistant Cultivars Challenges the Profitability of Agronomic Crops
Robert Loring Nichols, Cotton Incorporated
4:25 P-15 Conventional Fungicide Resistance Management
George Musson, Bayer CropScience
4:50 Discussion

MAXIMIZING THE POWER OF ANIMAL CELL FACTORY
SPONSORED BY THE JAPANESE ASSOCIATION FOR ANIMAL CELL TECHNOLOGY

Convener:       Ken-Ichi Nishijima, Nagoya University

3:30 pm – 5:00 pm Animal Symposium Marina 6

Animal cells are used for the production of pharmaceutical proteins. Topics in this session include the current standards for using CHO cells, insect cells and transgenic chickens to produce commercial recombinant proteins into culture medium or egg white and several process improvements for future use. Various technologies to improve productivity and quality of the proteins will be discussed such as systematic gene mutation or cell cycle regulation aiming to establish good producers, control of intracellular protein folding or extracellular protein aggregation leading to dramatic increases in product yield, or modifications of N- and O-linked glycans that strongly affect protein activities.

3:30 A-12 Production of Virus-like Particles Using Insect Cells
Hideki Yamaji, Kobe University
3:48 A-13 Mammalian Cell Factory- CHO Cell and Its Application for Biopharmaceutical Production
Takeshi Omasa, Osaka University
4:06 A-14 Genome Wide Mutagenesis Approach for Development of High-performance CHO Cell Line
Takayuki Horiuchi, Chitose Laboratory Corporation
4:24 A-15 TAPBOOST Technology: Enhanced Production for Difficult-to-Produce Proteins
Akinori Hishiya, SOLA BioSciences
4:42 A-16 Generically Manipulated Chickens as Animal Bioreactor
Ken-ichi Nishijima, Nagoya University


NON-COMPETITIVE STUDENT ORAL PRESENTATIONS

Moderators:    Jordan Brungardt, Kansas State University, and Matt Desrosiers, Worcester Polytechnic Institute

3:30 pm – 5:00 pm Education Workshop Harbor Island 1

Oral presentations are an intimidating and difficult part of graduate school for many students.  However, being able to communicate the results of experiments in an oral presentation is an essential skill as a scientist.  This session is a perfect opportunity for graduate students to practice their oral presentation skills in a low pressure setting and to receive valuable feedback from SIVB members.

3:30 Introduction (J. Brungardt and M. Desrosiers)
3:35 P-2013 Abstract has been withdrawn
3:55 P-2039 Light Intensity Stress as the Limiting Factor in Micropropagation of Sugar Maple (Acer saccharum Marsh.)
Amritpal Singh, University of Guelph, A. Maxwell P. Jones, and Praveen K. Saxena
4:15 P-2034 Downregulation of MTHFR and COMT in Switchgrass Decreases Lignin Content and Induces Lesion-mimic Cell Death
Sijia Liu, The Samuel Roberts Noble Foundation, Chunxiang Fu, Jiqing Gou, Yunwei Zhang, and Zeng-yu Wang
4:35 P-2036 Efficient Gene Delivery by Microinjection on Shoot Apical Meristems of Watermelon
A. Vinoth, Loyola College, R. Ravindhran, G. Ganesan, M. Harikrishnan, and Ajay Parida
4:55 Discussion


NOVEL IDEAS FOR PRODUCT DEVELOPMENT IN PLANTS

Conveners:     Ming Cheng, BASF Plant Sciences, and Ian S. Curtis

3:30 pm – 5:00 pm Plant Symposium Harbor Island 3

Plants can play an important role in topical issues such as climate change, food security, pest and disease control and production of renewable feedstocks. In terms of renewable chemicals, plants have distinct advantages over microbial and animal systems such as low cost production and freedom from animal pathogens. In spite of the significant potential of genetically engineering plants for improving crop yield and renewable raw materials for industry, there remains a deeper understanding of metabolic metabolism, gene regulation and cell to cell signaling pathways. Recent discoveries from genetic variants or direct gene manipulation studies in bioenergy crops have resulted in renewable feedstocks with improved recovery downstream conversion and yield. This session will focus on the developments on how plant systems can be manipulated for the production of novel plant products and its potential to compete towards the industrial global market.

3:30 Introduction (M. Cheng)
3:35 P-17 Glycoengineering of Plants to Produce Cytoprotective Asialoerythropoietin
Jiahua Xie, North Carolina Central University
4:00 P-18 Metabolic Network Based Regulation of Artemisinin Biosynthesis for Anti-malarial Medicine
De-Yu Xie, North Carolina State University
4:25 P-19 Sunflower as a Sustainable Crop for Commercial Rubber Production
Thomas Christensen, Edison Agrosciences, Inc
4:55 Discussion


THE ADVENT OF PRECISION BREEDING: AN ADVANCED METHOD FOR CROP GENETIC IMPROVEMENT

Convener:       Dennis J. Gray, University of Florida, Zhijian T. Li, University of Florida, and Sadanand A. Dhekney, University of Wyoming

5:00 pm – 6:00 pm Plant Symposium Harbor Island 3

Precision breeding (PB) is a newly-enabled approach to plant genetic improvement that transfers only specific desirable traits among sexually-compatible relatives. PB is a logical extension of conventional breeding (CB) inasmuch as it is fully consistent with the workings of the plant lifecycle. PB builds upon decades of both fundamental and applied research aimed at bypassing the disruption of trait recombination caused by the sexual reproduction process (meiosis) by allowing gene insertion to be accomplished via the significantly more stable and predictable mitotic cell division pathway. For an increasing number of crop plants, it is now possible to transfer only pre-evaluated genes and other genetic elements that express known traits among sexually-compatible relatives. PB allows genetic improvement to be accomplished “precisely” by making changes only to desired traits, while leaving the rest of the plant’s favored qualities intact; this is impossible with conventional, meiosis-driven, breeding. The foundational technologies that enable PB, including transformation and regeneration of entire plants via cell culture, have been shown repeatedly over several decades to be safe. The crops commercialized worldwide have been proven to be as safe as their conventionally-developed counterparts. Yet, the “controversies” continue to rage seemingly unabated as we ourselves continue to focus backwards to explain these decades-old technologies utilizing scientifically-incorrect terminologies. It now is proper to adopt teachings and discussions that focus within the well-established frameworks of botany, plant genetics and breeding. Concomitantly, certain key, but scientifically indefensible, terms that are in pervasive use today by both scientists and consumers alike (i.e., “GM”, “GMO” and “non-GM”) must be discarded and replaced with terms that do not mislead; that are scientifically accurate; and that allow logic and fact-based discussions to occur. This workshop will cover the development of precision breeding, its applications already being demonstrated in apple, grape and potato, and its implications relative to future regulatory requirements in the USA.

5:00 Introduction (D. J. Gray, Z. T. Li, and S. A. Dhenkey)
5:05 P-20 The New Biology of Precision Breeding
Dennis J. Gray, University of Florida
5:20 P-21 An Update on the Development of Commercial Crop Cultivars Using Precision Breeding
Sadanand Dhekney, University of Wyoming
5:35 P-22 Are You Regulated? Regulatory Issues Associated with Precision Breeding Technology
John Cordts, Cordts Consulting LLC
5:50 Discussion


IN VITRO ANIMAL CELL SCIENCES AND EDUCATION CONTRIBUTED PAPERS

Moderator:      Vivian Dayeh, University of Waterloo

5:00 pm – 6:00 pm Animal and Education Contributed Paper Session Marina 6
5:00 A-1004 Advantages of Cell Culture Microrheology for the Evaluation of Microtubule Stabilizing Drugs
Hannes Engelhardt, Friederich-Alexander Universität Erlangen-Nürnberg, Anja Limmer, Haider Dakhill, Hoger Hübner, Daniel Gilbert, and Andreas Wierschem
5:15 A-1006 Neconicotinoid Effects on Fish Cell Lines: Cautionary Effects on Non-target Cells and Species
Harshraj S. Sidhu, University of the Fraser Valley, Gagandeep Rai, Kamal Moghrabi, Vishesh Oberoi, and Lucy Lee
5:30 A-1005 A Training Based Internship Model in Plant Transformation for Undergraduates
Albert Kausch, University of Rhode Island, Joel Hague, Eric Oladapo, Kwesi Lillard, Mark Zingarelli, Moses Janga, Taryn Jacolucci, and Kimberly Nelson
5:45 A-1003 Gamification in Biology Courses: What’s Up with IF-AT?
Vivian Dayeh, University of Waterloo


PRODUCT APPLICATIONS, MICROPROPAGATION AND OTHER IN VITRO PLANT TOPICS

Moderator:      Lisa Lee, The Scotts Miracle-Gro Company

5:00 pm – 6:15 pm Plant Contributed Paper Session Harbor Island 2
5:00 P-1012 A Novel Micropropagation System for Moringa – A High Potential Nutritional and Pharmacologically Important Plant
Sudhersan Chellan, Kuwait Institute for Scientific Research, Sudhersan Jibi, Jawad Ashkanani, Aisha H. Al-Shatti, and Salah Al-Melhem
5:15 P-1013 Strong Cytotoxic Potential and Anti-Proliferative Effect of Nardostachys jatamansi D.C. Herbal Extract on Human Glioblastoma Cell Lines Manifested as Cell Cycle Arrest and Mitotic Catastrophe
Himanshi Kapoor, University of Delhi, and Veena Agrawal
5:30 P-1014 Meta-topolin Significantly Enhances De Novo Shoot Organogenesis and Plant Regeneration In Cassava, Sweetpotato and Soybean
Raj Deepika Chauhan, Donald Danforth Plant Science Center, and Nigel Taylor
5:45 P-1015 Conservation of Musa sp. seeds via Cryopreservation
Ergun Kaya, Mugla Sitki Koçman University, Fernanda Viigal Duerta Souza, Muammer Ceylan, and Maria Jenderek
6:00 P-1020 Purple is the New Orange – Next Generation Smart Fruits
Kasturi Dasgupta, USDA-ARS/University of California, and James Thomson

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