2016 World Congress on In Vitro Biology

For your viewing convenience, the 2016 World Congress on In Vitro Biology Final Program has been broken down by day.

Keynote Speaker

Seeing Single Molecules, from Early Spectroscopy in Solids, to Super-resolution Microscopy, to 3D Dynamics of Biomolecules in Cells.
William E. Moerner, Stanford University, Department of Chemistry

W_E_Moerner_081206-003-forwebDr. William E. Moerner, the Harry S. Mosher Professor of Chemistry and Professor, by courtesy, of Applied Physics at Stanford University, conducts research in physical chemistry and chemical physics of single molecules, single-molecule biophysics, super-resolution imaging and tracking in cells, and trapping of single molecules in solution. His interests span methods of precise quantitation of single-molecule properties, to strategies for three-dimensional imaging and tracking of single molecules, to applications of single-molecule measurements to understand biological processes in cells, to observations of the photodynamics of single photosynthetic proteins and enzymes. He has been elected Fellow/Member of the NAS, American Academy of Arts and Sciences, AAAS, ACS, APS, and OSA. Major awards include the Earle K. Plyler Prize for Molecular Spectroscopy, the Irving Langmuir Prize in Chemical Physics, the Pittsburgh Spectroscopy Award, the Peter Debye Award in Physical Chemistry, the Wolf Prize in Chemistry, and the 2014 Nobel Prize in Chemistry.

Abstract:
More than 25 years ago, low temperature experiments aimed at establishing the ultimate limits to optical storage in solids led to the first optical detection and spectroscopy of a single molecule in the condensed phase. At this unexplored ultimate limit, many surprises occurred where single molecules showed both spontaneous changes (blinking) and light-driven control of emission, properties that were also observed in 1997 at room temperature with single green fluorescent protein variants. In 2006, PALM and subsequent approaches showed that the optical diffraction limit of ~200 nm can be circumvented with single molecules to achieve super-resolution fluorescence microscopy with relatively nonperturbative visible light. Essential to this is the combination of single-molecule fluorescence imaging with active control of the emitting concentration and sequential localization of single fluorophores decorating a structure. Super-resolution microscopy has opened up a new frontier in which biological structures and behavior can be observed in fixed and live cells with resolutions down to 20-40 nm and below. Examples range from protein superstructures in bacteria to details of the shapes of amyloid fibrils and much more. Current methods development research addresses ways to extract more information from each single molecule such as 3D position and orientation, and both of these can be obtained by proper point-spread function engineering of a wide-field microscope. It is worth noting that in spite of all the current focus on super-resolution, even in the “conventional” low concentration, single-molecule tracking regime where the motions of individual biomolecules are recorded rather than the shapes of extended structures, much can still be learned about biological processes. Examples to be presented include studies of the primary cilium and motions of DNA loci in the nucleus.

Daily Program
Abstracts & Posters
Keynote Speaker Index Plant Symposia & Workshops
Saturday, June 11 Plenary Symposia Plant Contributed Papers
Sunday, June 12 Keynote Symposium Plant Posters
Monday, June 13 Animal Posters Education Posters
Tuesday, June 14 Joint Symposia Animal & Education Contributed Papers
Wednesday, June 15 Animal Symposia & Workshops Addendum Booklet
International Conference Symposia

 

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