Sunday, May 31

For your viewing convenience, the 2015 In Vitro Biology Meeting Final Program has been broken down by day.

Daily Program
Abstracts & Posters
Keynote Speaker Keynote Symposium Animal Contributed Papers
Saturday, May 30 Plenary Symposia Plant Contributed Papers
Sunday, May 31 Animal Symposia & Workshops Animal Posters
Monday, June 1 Joint Symposia Education Posters
Tuesday, June 2 Plant Symposia & Workshops Plant Posters
Wednesday, June 3 Index Addendum Booklet


Sunday, May 31

Daily Program-at-a-Glance

Time Event Location
7:00 am – 5:30 pm Registration Presidio Foyer Registration Area
10:00 am – 2:00 pm
5:30 pm – 7:30 pm
Exhibits and Posters Turquoise Ballroom
Morning 7:00 am – 12:30 pm
7:00 am – 9:00 am In Vitro Animal Cell Sciences Program Committee Meeting Joshua Tree 1
Plant Biotechnology Program Committee Meeting Coronado Ballroom
8:00 am – 10:00 am The Stem Cell State – Implications for Growth and Disease Presidio V
10:00 am – 10:30 am Coffee Break Turquoise Ballroom
Nominating Committee Meeting Joshua Tree 2
Public Policy Committee Meeting Joshua Tree 1
10:30 am – 12:30 pm Advances in Plant Propagation Presidio V
Genome Editing Presidio III/IV
Complex Tissue Structures: Insights and Applications of Human Organoid Cultures Agave Ballroom
11:00 am – 12:00 pm SIVB/IAPB/Springer Business Meeting Joshua Tree 2
Afternoon 12:30 pm – 6:30 pm
12:30 pm – 1:30 pm Membership Committee Meeting Joshua Tree 1
In Vitro Plant Editorial Board Meeting Joshua Tree 2
Exhibitors/SIVB Refreshment Break Turquoise Ballroom
1:30 pm – 3:30 pm Bioengineering, Microfluidics, and In Vitro Imaging Agave Ballroom
Micropropagation as a Useful Tool for Addressing Global Megatrends Presidio V
Mutagenesis Presidio III/IV
3:15 pm – 5:00 pm 2015 In Vitro Biology Meeting Opening Ceremony

3:15 Welcome and Opening Remarks
3:25 Robert H. Lawrence, Jr. Keynote Symposium
4:30 2015 Society for In Vitro Biology Awards Ceremony
4:35 2015 Fellow and Young Scientist Award Recipients (Awards to be presented at Section Meetings)
4:40 2015 Distinguished Service Award Presentations
5:00 2015 SIVB Lifetime Achievement Award Presentations
Presidio V
5:30 pm – 6:30 pm 2015 In Vitro Biology Meeting Opening Ceremony Reception Turquoise Ballroom
Evening 6:30 pm – 9:30 pm
6:30 pm – 7:30 pm Poster Session
Odd Poster Authors will be present
Turquoise Ballroom
7:30 pm – 9:30 pm Student Symposium: Interview Coronado Ballroom

Sunday, May 31

Program is subject to change
7:00 am – 5:30 pm Registration Presidio Foyer Registration Area


Conveners: Michael K. Dame, University of Michigan Medical School, and William Gordon-Kamm, Pioneer Hi-Bred International

8:00 am – 10:00 am Plenary Symposium Presidio V

Multicellular development, regeneration, aging, disease: all have as a central player the stem cell, both normal and aberrant. Understanding the cell of origin and its dynamic niche has profound implications for applications as diverse as organ replacement, cancer therapy and modulating plant morphogenesis. This session has the unique opportunity to juxtapose cutting edge insights of human stem cell and plant meristem cell biology, and to potentially generate fascinating insights into the fundamental nature of one another’s function.

8:00 Introduction (M. K. Dame and W. Gordon-Kamm)
8:05 PS-1 In Vitro Generation of Human Pluripotent Stem Cell Derived 3-dimensional Organoids for Studying Human Development and Disease
Jason R. Spence, University of Michigan
8:40 PS-2 New Regulators of Shoot Meristem Development in Maize and Arabidopsis, and Potential Benefits for Crop Yields
David Jackson, Cold Spring Harbor Laboratory
9:15 PS-3 Transcriptional Logic of Plant Stem Cell Maintenance
Venugopala Gonehal, University of California – Riverside
9:50 Discussion
10:00 am – 10:30 am Coffee Break Turquoise Ballroom


Convener: Wayne Curtis, Penn State University and Jeffrey Beringer, Dow AgroSciences

10:30 am – 12:30 pm Plant Symposium Presidio V

Plant propagation methods have advanced rapidly over the past 50 years – aided first by in vitro methods, and more recently with the advances of “omics” and bioinformatics. The advantages of laboratory based plant propagation include the mass production of plantlets from species that produce few seeds, are sterile, or require extended times for maturation. In vitro propagation methods also facilitate the production of disease free plantlets, and phenotypic uniformity (cloning). Starting with ornamentals and progressing toward forestry plants and high value fruits and vegetables, industrial labs have sought to provide growers with high quality plantlets at as low a unit cost as possible. This session will highlight recent developments in the methods used to drive further efficiency improvements. Presenters from industry labs and academic partners will review improvements in automation, bioreactor systems, somatic embryo production, and the reduction of inputs required for healthy plant production.

10:30 Introduction (W. Curtis and J. Beringer)
10:35 P-1 Advances in Research Methods for Commercial Micropropagation Systems
Brett Gytri, Tissue-Grown Corporation
11:00 P-2 Temporary Immersion Bioreactor & Somatic Embryogenesis in Micropropagation
Sergio Florez, Penn State University
11:25 P-3 Progress on Scale-up Somatic Embryogenesis and Manufactured Seed Technology of Conifer Species at Weyerhaeuser
Pramod Gupta, Weyerhaeuser Technology Center
11:50 P-4 Photoautotrophic (Sugar-free Medium) Micropropagation – Its Principle and Application
Toyoki Kozai, Chiba University
12:15 Discussion


Conveners: Qiudeng Que, Syngenta Biotechnology, Inc., and Prakash Kumar, National University of Singapore

10:30 am – 12:30 pm Plant Symposium Presidio III/IV

Recent development of programmable site-directed nucleases including TALEN and CRISPR-Cas9 has made it routine to perform precise manipulation in many plant and animal genomes. However, even though the basic molecular tools for targeted mutagenesis are already in place, there are still a lot of protocol development and optimization that need to be done to make allele replacement efficient and economical in crops with complex genomes. Also, for commercial product development, new options of nucleases and delivery methods might be needed to facilitate regulatory approval. In this session, the speakers will discuss the use of several different site-directed nucleases for various genome editing applications including targeted mutagenesis, allele replacement and targeted insertion in both model plants and crops with complex genomes such as corn and sugarcane. The session will also discuss the basic mechanisms of DNA repair behind the different genome editing processes.

10:30 Introduction (Q. Que and P. Kumar)
10:35 P-5 Double-strand Break Induced Genome Engineering in Plants
Holger Puchta, Karlruhe Institute of Technology
11:00 P-6 Developing Toolkits for Plant Genome Editing and Transcriptional Regulation
Yiping Qi, East Carolina University
11:25 P-7 TALEN and CRISPR-Cas9 in Rice and Disease Control
Bing Yang, Iowa State University
11:50 P-8 Author is unable to attend
12:20 Discussion


Conveners: Yan Jiang, MD Anderson Cancer Center, and Michael K. Dame, University of Michigan Medical School

10:30 am – 12:30 pm Animal Symposium Agave Ballroom

Organoid cultures recapitulate in vivo tissue histology and stem cell lineage hierarchy. These complex, multicellular models derived from contemporary patients or animals yield superior data quality and more accurate tissue-specific information to significantly improve the predictive value and translation between in vitro, animal, and clinical human studies. In this session, we will discuss successful applications of organoid culture: a kidney organoid culture, contrasted with conventional 2-D cell culture, to assess drug toxicity of common drugs and to characterize clinically relevant markers; organoids of patient-derived colon neoplastic tissue, with phenotypic and genomic comparisons to it’s in situ counterpart; and finally a mammary organoid model used to understand how the 3-D microenvironment interacts with the stem cell niche and signaling.

10:30 Introduction (Y. Jiang and M. K. Dame)
10:35 A-1 Comparing 2D Kidney Cell and 3D Kidney Organoid Cultures in Drug Toxicity Assays
David W. Grainger, The University of Utah
11:10 A-2 Phenotypic & Genomic Characterization of Organoid Cultures Derived from Colonic Neoplastic Tissues
Michael K. Dame, University of Michigan Medical School
11:45 A-3 Microenvironmental Control Networks in the Mammary Stem Cell Niche
Kai Kessenbrock, University of California-San Francisco
12:20 Discussion


Conveners: Joshua Z. Gasiorowski, Midwestern University, and Chunyan Wu, Cornell University

1:30 pm – 3:30 pm Animal Symposium Agave Ballroom

Studying complex physiological systems in vivo, especially at the cellular level, can be a significant challenge. The relatively young interdisciplinary field of bioengineering has enhanced our ability to observe cell morphology, cell-cell interactions, organogenesis, and tissue development through new, in vitro technological advancements. This session will focus on two of these advancements: microfluidics and in vitro imaging. Microfluidic systems have the potential to miniaturize and replicate multicellular tissue and organoid structures with lab-on-a-chip/tissue-on-a-chip applications for in vitro investigation. New advancements in live cell imaging have proven to be a tremendous complementary method for microfluidic systems and can help us elucidate cellular and tissue development mechanisms in real-time. Both applications have shown promise to accelerate biological discoveries and improve clinically relevant diagnostic tests.

1:30 Introduction (J. Z. Gasiorowski and C. Wu)
1:35 A-4 Epithelial Explants from Adult Zebrafish: An Experimental System to Image Epithelial Wound Healing in a Tissue Context
Elizabeth E. Hull, Midwestern University
2:15 A-5 Smartphone Biosensors and Organ-on-a-Chip
Jeong-Yeol Yoon, The University of Arizona
2:55 Discussion


Conveners: Mary Welter, Dow AgroSciences, and John Bijl, Vitro Plus

1:30 pm – 3:00 pm Plant Symposium Presidio V

Various Megatrends have been identified that affect the world population. These include the following: A. Growing Demand of Food: Worldwide consumption of food will increase with some 45% over the next 40 years; with increasing wealth of individuals, the average consumption per person will increase approximately 10%; as shift in the consumption patterns from commodity types of food to richer and more healthy food; and the world urban population is expected to increase by 72% over the next 40 years, particularly in urban areas of less developed regions and B. Constrained Environmental Resources: Limitations of arable land and fresh water resource require revolutionary innovation in food production and the consumer demands more control, traceability, elimination of contamination and reduction of spills. We know that tissue culture can contribute to develop and find solutions to overcome these future challenges. This session will feature speakers that use micropropagation of commercial crops that include energy crops, edibles or high value food products that address some of these trends.

1:30 Introduction (M. Welter and J. Bijl)
1:35 P-9 Support of Nestlé’s Nescafé and Cocoa Plans Using Plant Tissue Culture
Jean-Paul Ducos, Nestle
2:00 P-10 A Simplified Technique for the Propagation of Shoots from Nodes of Switchgrass (Panicum virgatum L.) Genotypes
Joshua Weaver, The University of Arizona
2:25 P-11 Sugarcane Technology Center – Development of Innovative Technologies to Increase Agricultural Efficiency of the Sugarcane Industry
Danila Montewka Melotto Passarin, Center for Technology, Canavieira, Brazil
2:50 Discussion


Conveners: Ming Cheng, BASF Plant Science, and Mindy Fitter, KWS Gateway Research Center

1:30 pm – 3:00 pm Plant Symposium Presidio III/IV

Conventional induced mutagenesis has been widely used in crop breeding and has resulted in the development and release of many new cultivars for commercial cultivation. In vitro mutagenesis combined with plant cell and tissue culture techniques offers unique advantages over conventional mutagenesis because it allows for efficient generation and selection of mutants by easy handling of large cell population, early selection in plant morphogenesis, as well as rapid cloning of selected mutants. This approach has been employed to create new agronomic traits such as herbicide and fungal tolerant crops both in private industry and academic research labs. Induced mutation has also been widely used in functional genomics. For example, a reverse genetics tool TILLING (Targeting Induced Local Lesions IN Genomes) involving chemical mutagenesis has been developed to generate a population of plants with random point mutations followed by high throughput screening for point mutations in a target gene in order to understand the function of the mutant genes. This session will highlight mutagenesis approaches for crop improvements.

1:30 Introduction (M. Cheng and M. Fitter)
1:35 P-12 Fully Sequence-indexed Collections of Chemically-mutagenized Plants: a Tool for Understanding Gene Function
Clifford Weil, Purdue University
2:00 P-13 Sugarcane Mutation Breeding in South Africa: Progress and Prospects
Richard S. Rutherford, The South African Sugarcane Research Institute
2:25 P-14 Tissue Culture Mutagenesis as a Tool for Developing Herbicide Tolerant Traits in Crops
Jill Stevenson-Paulik, BASF Plant Science
2:50 Discussion


Program Chair: John W. Harbell, JHarbell Consulting

3:15 pm – 5:30 pm Opening Ceremony Presidio V
Conveners: John W. Harbell, JHarbell Consulting, and Eugene Elmore, University of California – Irvine
3:15 Welcome and Opening Remarks:
Eugene Elmore, President, Society for In Vitro Biology
3:25 KS-1 Introduction (E. Elmore)
Engineering Gastrointestinal Cancer in Organoid Cultures
Calvin Kuo, Stanford University School of Medicine
4:15 Questions and Answers
4:35 2015 Fellow and Young Scientist Award Recipients (Awards to be presented at Section Meetings)

Fellow: Harold Trick, Kansas State University
Distinguished Scientist: Zeng-Yu Wang, The Samuel Roberts Noble Foundation
4:40 2015 Distinguished Service Award Presentations
(Awards to be presented by Eugene Elmore):Ad Hoc Website Committee
Wayne A. Parrott, University of Georgia
Michael J. Fay, Midwestern University
Michael E. Kane, University of Florida
Sylvia A. Mitchell, University of the West Indies
Dwight T. Tomes
Jeffrey Kwak
James J. Sadler,
University of Florida
Nguyen H. Hoang, University of Florida
Daniel J. Barnes, Southeastern Louisiana UniversitySupport of the Society Mission
Marten Peterson
David D. Songstad,
Cibus, LLC
5:00 2015 Lifetime Achievement Award Presentations:
(Introduction by Sandra Schneider; Acknowledgement by Dr. Buehring to follow)
Gertrude C. Buehring
5:15 (Introduction by Eugene Elmore; Acknowledgement by Dr. Bethell to follow)
Delia R. Bethell
5:30 Adjourn


5:30 pm – 7:30 pm Exhibits and Posters Turquoise Ballroom
6:30 pm – 7:30 pm Odd Poster Authors will be present Turquoise Ballroom


Conveners: Shaimar González, Marshall University, and Ningning Zhang, Arkansas State University

7:30 pm – 9:30 pm Education Symposium Coronado Ballroom

The interview is one of the most important steps in the job search process. It is your chance to elaborate on how your education, skills, and experience fit what the employer is seeking in a candidate and ultimately gets you the job offer. Developing good interviewing skills is essential to make you a stronger candidate for the job outside of your CV/resume. This interactive symposium is designed to help students to learn and improve their interviewing skills, particularly those students with little to none interview experience. We have invited two main speakers from both industry and academia to share their experience and give us general interview tips. This will be followed by a panel discussion session where six advisory consultants (including two main speakers) will answer specific questions. This session will train students to be better prepared for their future job interview.

7:30 Introduction (S. González and N. Zhang)
7:35 E-1 Internship Opportunities at Pioneer DuPont
Theodore M. Klein, Pioneer Crop Genetics
8:00 E-2 Winning the Job Interview: An Interactive Experience
Magnolia Ariza-Nieto, Cornell University
8:25 Panel Discussion
Magnolia Ariza-Nieto, Cornell University
Wayne Curtis, Penn State University
Theodore M. Klein, Pioneer Crop Genetics
Patrick McNutt, US Army Medical Research Institute of Chemical Defense
Paul Price, Past President of SIVB
Dwight T. Tomes, Pioneer Hi-Bred Intl. Inc


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