Tuesday, June 3
Daily Program-at-a-Glance
| Time | Event | Location |
|---|---|---|
| 7:00 am – 5:00 pm | Registration | Registration Booth |
| 10:00 am – 3:30 pm | Exhibits and Posters | Harborside Center |
| Morning | 7:00 am – 12:30 pm | |
| 7:00 am – 8:00 am | In Vitro–Animal Editorial Board Meeting | Verelst |
| SIVB Student Affairs Breakfast | Percival/Vernon | |
| 8:00 am – 10:00 am | Genome Editing/Gene Delivery/Targeted Mutagenesis |
Regency AB |
| 10:00 am – 10:30 am | Coffee Break | Harborside Center |
| SIVB Awards Committee Meeting | Verelst | |
| SIVB Constitution and Bylaws Committee Meeting | Westbrook | |
| 10:30 am | Closing of the Creative Coast Silent Auction | Harborside Center |
| 10:30 am – 12:30 pm | Adding Value to Bioenergy Crops Via Gene Technology | Regency DEF |
| Advances in SiRNA and MicroRNA Technologies |
Regency C | |
| Evidence-Based Best Practices For Biorepositories |
Savannah | |
| Osmotic Stress and Cryoprotectant Toxicity |
Percival/Vernon | |
| Precision Genome Modification in Crop Plants | Regency AB | |
| Afternoon | 12:30 pm – 6:30 pm | |
| 12:30 pm | Announcement of the Creative Coast Silent Auction Winners | Registration Booth |
| 12:30 pm – 1:30 pm | 2015 SIVB Program Planning Committee Meeting | Westbrook |
| SIVB Development Committee Meeting | Verelst | |
| 1:30 pm – 2:30 pm | Publication Ethics | Regency AB |
| Interactive Poster Sessions: In Vitro Animal Cell Sciences Transformation And Gene Expression |
Scarborough 3/4 | |
| 2:30 pm – 3:30 pm | Poster Session
Odd Poster Authors Will be Present |
Harborside Center |
| 3:30 pm – 5:00 pm | Epigenetics and Epigenetic Markers In Human Health |
Regency C |
| From Cryopreservation to Preservation in a Dry State | Savannah | |
| Micropropagation and Cryopreservation of Tropical Plant Species | Regency AB | |
| Oocyte and Gamete Cryopreservation | Percival/Vernon | |
| Plant Transformation Improvements | Regency DEF | |
| 5:00 pm – 5:30 pm | SIVB Business Meeting Student Award Presentation |
Regency C |
| Evening | 6:30 pm – 9:00 pm | |
| 5:45 pm – 9:00 pm | An Evening At Old Fort Jackson | Old Fort Jackson |
Tuesday, June 3
| 7:00 am – 5:00 pm | Registration | Registration Booth |
Genome Editing / Gene Delivery / Targeted Mutagenesis
Conveners: David D. Songstad, Cibus, LLC, and Joshua Z. Gasiorowski, Midwestern University
| 8:00 am – 10:00 am | Plenary Symposium | Regency AB |
For several decades now, researchers have been able to successfully deliver exogenous DNA into a wide variety of cells and organisms. This ability to overexpress and knock down specific genes via the introduction of transgenic material has been a powerful tool for perturbing genetic systems for basic science and translational applications. Despite the continued advancement of gene delivery techniques, being able to precisely target and alter endogenous DNA has remained a significant challenge. In this session, the speakers will discuss relatively new methods to modify and edit a host cell’s chromatin at specific loci using engineered enzymes, such as transcription activator-like effector nucleases (TALENs) and zinc finger nucleases (ZFNs), as well as oligonucleotide directed mutagenesis. These new technologies have allowed scientists to design well controlled experiments in the framework of the endogenous genome and hold significant promise for future gene therapy strategies and agricultural product development.
| 8:00 | Introduction (D. D. Songstad and J. Z. Gasiorowski) | |
| 8:05 | PS-7 | Rapid Targeted Genome Modification in Mice, Rats and Rabbits Ed Weinstein, SAGE lab in Saint Louis |
| 8:40 | PS-8 | Precise Engineering of Genomes with Sequence Specific Nucleases Dan Voytas, University of Minnesota |
| 9:15 | PS-9 | RTDS™ – an Oligonucleotide-Directed Mutagenesis for Trait Development Christian Schopke, Cibus, LLC |
| 9:50 | Discussion |
| 10:00 am – 10:30 am | Coffee Break | Harborside Center |
Adding Value to Bioenergy Crops via Gene Technology
Conveners: Fredy Altpeter, University of Florida, and Ian S. Curtis, Chromatin Inc.
| 10:30 am – 12:30 pm | Plant Symposium | Regency DEF |
Rising oil prices and the need for energy security have driven the world’s attention for creating alternative energy resources. Biofuels, a hydrocarbon made by or from a living or once living organism in a short period of time, is an attractive fuel substitute. These fuels are made from plant or plant-derived materials via thermal, chemical, and biochemical conversion processes. Bioethanol, made from the fermentation of carbohydrates in sugar or starch crops, is the major biofuel produced within the US. However, recent manufacture of bioethanol caused a ‘food vs fuel’ debate. Advances in the production of cellulosic ethanol from renewable lignocellulosic biomass has the potential to lead the bio-industrial revolution, as such sources have widespread abundance and are available at low cost. In this symposium, speakers will present their research on their specific target crop, demonstrating recent developments and feasibility of biofuel production.
| 10:30 | Introduction (I. S. Curtis) | |
| 10:35 | P-23 | Genetic Improvement of the Biofuel Feedstock Sugarcane with Intragenic, Targeted Mutagenesis and Transgenic Biotechnologies Fredy Altpeter, University of Florida |
| 11:00 | P-24 | Engineering Sesquiterpene-based Biofuels in Sorghum Ian S. Curtis, Chromatin Inc. |
| 11:25 | P-25 | Genetic Modification of Switchgrass for Improved Biofuel Production Zeng-Yu Wang, The Samuel Roberts Noble Foundation |
| 11:50 | P-26 | Planted Hardwood Bio-energy Forests for the Southeastern United States: Deployment Strategies in Hybrid Poplar and Eucalyptus sp. Jeff Wright, AborGen |
| 12:15 | Discussion |
Advances in siRNA and microRNA Technologies
Convener: Michael J. Fay, Midwestern University
| 10:30 am – 12:30 pm | Animal Symposium | Regency C |
RNA interference (RNAi) mediated by small interfering RNAs (siRNAs) or microRNAs is an effective mechanism to silence cellular gene expression at the posttranscriptional level. This session will focus on some of the diverse applications of siRNA- and microRNA-based technologies. Topics to be discussed during the session include: 1) RNAi-based pest control strategies for insects; 2) An RNAi-based screening approach to identify genes involved in viral replication; and 3) An RNAi-based therapeutic approach that utilizes engineered exosomes which deliver microRNAs to inhibit tumor cell growth.
| 10:30 | Introduction (M. J. Fay) | |
| 10:35 | A-11 | RNAi as New Target for Pest Control: Challenges with Insect Cell Cultures Guy Smagghe, Ghent University |
| 11:10 | A-12 | RNAi Technology to Understand the Virus-host Interface Ralph Tripp, The University of Georgia |
| 11:45 | A-13 | Engineered Microvesicles as Targeted Delivery Agents for Cancer Thomas Schmittgen, The Ohio State University |
| 12:20 | Discussion |
Evidence-based Best Practices for Biorepositories
Convener: Judith Giri, Centers for Disease Control and Prevention
Co-Convener: Fay Betsou, IBBL
| 10:30 am – 12:30 pm | Cryobiology Symposium | Savannah |
Biorepositories are the professional infrastructures responsible for the collection, processing, storage and distribution of biospecimens for research and clinical purposes. To maximize the quality and use of the specimens, they follow Best Practices and SOPs. Pre-analytical validation of sample quality is becoming increasingly important to make advances in global and public health. This session will highlight several initiatives in this topic and will be presented by members of the International Society for Biological and Environmental Repositories.
| 10:30 | C-30 | ISBER Best Practices in Action at CDC’s CASPIR Biorepository Judith Giri, Centers for Disease Control and Prevention |
| 11:00 | C-31 | Sample Processing & Handling Events: How a Sample is Defined L. Mark Cosentino, Biogen Idec, Inc. |
| 11:30 | C-32 | The Pan-Smithsonian Cryo-Initiative – Freezing for the Future Pierre Comizzoli, Smithsonian Conservation Biology Institute, National Zoological Park |
| 12:00 | C-33 | Best Practices for Archiving Environmental Samples for the National Institute of Standards and Technology’s Marine Environmental Specimen Bank (Marine ESB) Rebecca Pugh, National Institute for Standards and Technologies |
Osmotic Stress and Cryoprotectant Toxicity
Convener: Adam Higgins, Oregon State University
| 10:30 am – 12:30 pm | Cryobiology Symposium | Percival/Vernon |
In general, cell preservation involves manipulation of the solution composition to allow storage of the sample in a glassy state, typically by adding protectant solutes (e.g., glycerol, dimethyl sulfoxide or trehalose) and removing cellular water. This session will focus on the mechanisms of cell damage induced by osmotic stresses and exposure to cryoprotectants, as well as strategies for avoiding these damage mechanisms.
| 10:30 | C-34 | Coping with Water Loss: Protecting Your Proteome Under Stress Kevin Strange, MDI Biological Laboratory |
| 11:10 | C-35 | Lycoprotection During Bovine Sperm Dessication and Osmotic Damage Model Sankha Bhowmick, University of Massachusetts, Dartmouth |
| 11:30 | C-36 | Origins of Cryoprotectant Toxicity Greg Fahy, 21st Century Medicine, Inc. |
| 12:10 | C-37 | Kinetic Modeling of CPA Toxicity and Implications for Design of Cryopreservation Procedures Adam Higgins, Oregon State University |
Precision Genome Modification in Crop Plants
Conveners: Ted Klein, DuPont Pioneer Agricultural Biotechnology, and Dwight T. Tomes, Dupont Pioneer Agricultural Biotechnology
| 10:30 am – 12:30 pm | Plant Symposium | Regency AB |
Modification of crop plants via genetic transformation for various agronomic characteristics has been very successful commercially in North America but has met with resistance in Europe and some other countries with uncertainty concerning regulatory approval and public acceptance. Recent research in which genes are transferred between closely related organisms in a more precise and predictable manner, termed ‘cisgenesis’, offers promise for easing or eliminating regulatory hurdles both in North America and other countries. In addition, these sophisticated techniques would allow multiple changes at defined genetic loci in a given species. This session will include speakers who describe their research in adapting different techniques for plant improvement using cisgenics. These include zinc fingers with binding proteins that recognize specific sequences, meganucleases characterized by a large recognition site that can be used for repeated modification, and CRISPRs loci containing short recognition sites first described in microorganisms, but recently modified for use in plants.
| 10:30 | Introduction (T. Klein and D. T. Tomes) | |
| 10:35 | P-27 | Genome Editing in Plants Via Designed Zinc Finger Nucleases Joseph F. Petolino, Dow AgroSciences |
| 11:00 | P-28 | Targeted Trait Introduction and Mutagenesis in Crop Plants by Double-Strand-Break Technologies Mark Cigan, DuPont Pioneer |
| 11:25 | P-29 | Genome Editing in Plants Using CRISPR/Cas9/sgRNA Technologies Donald P. Weeks, University of Nebraska |
| 11:50 | P-30 | Nanoparticle Mediated Recombinase Delivery in Maize
Kan Wang, Iowa State University |
| 12:15 | Discussion |
Publication Ethics
Convener: Randall P. Niedz, USDA-ARS, Yan Jiang, MD Anderson Cancer Center
| 1:30 pm – 2:30 pm | Joint Workshop | Regency AB |
The session is a discussion of the various ethical issues involved in the publication of a scientific paper. The Editor of the plant section of the society and a representative from the publisher, Springer, will 1) focus the discussion using a panel format and, 2) provide their perspectives of the most persistent and serious issues. Scientific journals publish papers that describe research studies for the purpose of expanding knowledge. To aggregate information from individual studies into the knowledge of the larger scientific community, papers must describe experiments and report results accurately. This includes providing 1) the rationale and context of the experiment, 2) sufficient detail to repeat the experiment, and 3) data and data analyses that are fully described and accurately reported. Some ethical issues critical for maintaining the integrity of scientific publications includes plagiarism, data falsification, authorship, conflict-of-interest, and privileged information.
| 1:30 | Introduction (R. P. Niedz and Y. Jiang) | |
| 1:35 | John Finer, The Ohio State University | |
| 2:00 | Jacco Flipsen, Springer | |
| 2:25 | Discussion |
In Vitro Animal Cell Sciences Interactive Poster Session
Moderator: Michael K. Dame, University of Michigan Medical School
| 1:30 pm – 2:30 pm | Animal Interactive Poster Session | Scarborough 3/4 |
| A-2000 | Recombinant Nanobody™ Expression in Escherichia coli, Extraction and Purification Cletus Titanji Atta, Vrije Universitiet Brussels |
|
| A-2001 | Application of Impedance-based Cell Analysis for Evaluation of Phenotypic and Cellular Responses of Normal and Tumorigenic Rat Liver Epithelial Cells Brad L. Upham, Michigan State University, L. Ctverackova, Iva Sovadinova, James E. Trosko, and Pavel Babica |
|
| A-2002 | Recovery of Fibroblast-like Cells After 160 Days of Postmortem Storage of Goat Skin Tissues in Refrigerated Media Hadj Aoued, Fort Valley State University, and Mahipal Singh |
|
| A-2003 | The Mechanism of Redox Regulation in Animal Cells by Hydrogen and Pt Nanoparticles as Antioxidants Sanetaka Shirahata, Kyushu University, Takeki Hamasaki, Tomoya Kinjo, Hidekazu Nakanishi, Shigeru Kabayama, and Kiichiro Teruya |
Transformation and Gene Expression
Moderator: Shujie Dong, Syngenta Biotechnology
| 1:30 pm – 2:30 pm | Plant Interactive Poster Session | Scarborough 3/4 |
| P-2011 | Continued Improvement of Agrobacterium-mediated Sugar Cane Transformation Shujie Dong, Syngenta, Eder Bedani, John Ke, Pearl Mai, Graziela Baldacin, Paulo deLucca, Kris Mayo, G. O’hata-held, Manuel Sainz, Erik Dunder, and Jason Geijskes |
|
| P-2012 | Transient Expression of a Viral Insecticide in Plants Saranya Ganapathy, Texas Tech University, Megha Parajulee, Hong Zhang, and Shan Billmoria |
|
| P-2013 | Molecular Characterization of Transgenic ‘W.Murcott’ (Nadorcott) Mandarin Produced Using a Protoplast-GFP Transformation System Ahmad Omar, University of Florida, and Jude Grosser |
|
| P-2014 | Site-specific Integration of Transgenes into the Sugarcane Genome for Elimination of Transgene Position Effects Yang Zhao, University of Florida, Jae Yoon Kim, Je Hyeong Jung, Chnyang Fan, Wenjin Yu, Shujie Dong, Vibha Srivastava, and Fredy Altpeter |
|
| P-2015 | Functional Characterization of a Soybean SUMO E3 Ligase GmSIZ1-1 Zhigang Li, Clemson University, Qian Hu, Junming Zhao, Katherine Rebholz, Halina Knap, and Hong Luo |
| 2:30 pm – 3:30 pm | Odd Poster Authors will be present | Harborside Center |
Epigenetics and Epigenetic Markers in Human Health
Conveners: Brad L. Upham, Michigan State University and Barbara B. Doonan, New York Medical College
| 3:30 pm – 5:00 pm | Animal Symposium | Regency C |
The term Epigenetics was first coined by Conrad Waddington in 1942. The development and maintenance of an organism is coordinated by sets of molecular events that switch parts of the genome off and on at strategic times and locations during the life of an organism. Epigenetics is the study of these molecular events, in essence a genetic fine tuning – production of heritable changes in genome expression and cellular function brought about without any changes in DNA sequence. This relationship between the genotype and the resulting phenotype of an organism is dependent upon various intrinsic and extrinsic molecular events such as the methylation of DNA, histone alterations, and a variety of RNAs in different forms – RNAi, miRNAs, long non coding RNAs, piRNAs and link RNAs. The perinatal environment has been observed to play a critical role in human early development which can influence the risk of disease manifestation in the adult, known as the “Barker Hypothesis”. Also a growing body of epidemiological research has demonstrated the important epigenetic connection between nutrition and health. In this session the focus will be on the environmental epigenetic influences pertaining to humans and health.
| 3:30 | Introduction (B. L. Upham and B. B. Doonan) | |
| 3:35 | A-14 | The Language, Molecular Players, and Regulation of Epigenetics Joseph M. Wu, New York Medical College |
| 4:00 | A-15 | Epigenetic Predictors of Child Behavior: From Prenatal Exposures to Psychopathology Alicia K. Smith, Emory University School of Medicine |
| 4:25 | A-16 | Genome-wide Alteration of 5-hydroxymethylcytosine in a Mouse Model of Fragile X-associated Tremor/Ataxia Syndrome Bing Yao, Emory University School of Medicine |
| 4:50 | Discussion |
From Cryopreservation to Preservation in a Dry State
Convener: Wim Wolkers, Leibniz Universität Hannover
| 3:30 pm – 5:00 pm | Cryobiology Symposium | Savannah |
Stabilization of cells or tissues in the dry state is of considerable practical significance since samples can be stored at room temperature for long periods. Cryopreservation, however, is still the standard in biobanking of cells and tissues. The aim of this session is to establish the crucial steps needed to store samples in a dry state and move away from cryopreservation. The following topics will be covered: (1) anhydrobiosis: lessons from nature, (2) stabilizers for dry storage, (3) specific issues with stabilizers: loading in cells, diffusion in tissues (4) biophysics of freeze-drying, (5) other drying techniques. The feasibility of freeze-drying will be explored going from relatively simple (biomolecular assemblies, cellular fragments) to more complex systems (cells and tissues).
| 3:30 | C-38 | Preservation of Mammalian Sperm by Freeze-drying Takehito Kaneko, Kyoto University |
| 3:50 | C-29 | TBD Amir Arav, FertileSafe Ltd. |
| 4:10 | C-40 | Author is unable to attend |
| 4:30 | C-41 | In-situ Determination of the Relaxation Characteristics of Sugar Glasses Embedded in Microfiber Substrates Lindong Weng, University of North Carolina at Charlotte |
| 4:45 | C-42 | Measurement of Intracellular Water and Trehalose in Dessicated Cells Alireza Abazari, Massachusetts General Hospital, Steven Hand, Alptekin Aksan, and Mehmet Toner |
Micropropagation and Cryopreservation of Tropical Plant Species
Conveners: Barbara M. Reed, USDA-ARS National Clonal Germplasm Repository, and Sylvia A. Mitchell, University of the West Indies
| 3:30 pm – 5:00 pm | Plant Symposium | Regency AB |
Tropical plants provide many challenges in the fields of in vitro culture and cryopreservation. The high level of internal bacteria and fungal mycelium in tropical plants, especially after rainy, humid episodes, makes it difficult to initiate cultures. Many tropical plans lack desiccation and cold tolerance and this limits their cryopreservation potential. This session will present some of the techniques used to overcome some of these problems and provide for medium to long-term storage of tropical crops.
| 3:30 | Introduction (B. M. Reed and S. A. Mitchell) | |
| 3:35 | P-31 | Innovative Ways to Successfully Initiate Tropical Plant Species Including Ginger (Zingiber Officinale), Turmeric (Curcuma Longa), Sarsaparilla and Chainy Root (Smilax Spp), and Yam (Dioscorea Spp) Sylvia Mitchell, University of the West Indies |
| 3:55 | P-32 | Micropropagation of Ornamental Tropical Foliage Plants: Not Just Multiplication Jianjun Chen, University of Florida |
| 4:15 | P-33 | Cryopreservation of Woody Tropical Plants Ana Abdelnour Esquivel, Technological Institute of Costa Rica |
| 4:35 | P-34 | Cryopreservation of Orchid Seeds, Protocorms and Pollen Wagner A. Vendrame, University of Florida |
| 4:55 | Discussion |
Oocyte and Gamete Cryopreservation
Moderator: David Rawson, University of Bedfordshire
| 3:30 pm – 5:00 pm | Cryobiology Contributed Paper Session | Percival/Vernon |
| 3:30 | C-1013 | Cholesterol-loaded Cyclodextrin Improves Goat Semen Cryosurvival by Enhancing Sperm Cholesterol Content and Osmotic Tolerance Vianney Salmon, CRBR, Pierre Leclerc, and Janice Bailey |
| 3:45 | C-1014 | Cryopreservation of Spermic Milt in the Model Species Ambystoma tigrinum (Tiger Salamander) for Application in Endangered Salamanders Ruth Marcec, Mississippi State University, Cecilia Langhorne, Carrie Vance, Andrew Kouba, and Scott WIllard |
| 4:00 | C-1015 | High Survival of Mouse Oocytes and Preimplantation Embryo After Vitrification in the Absence of Permeating Cryoprotectants and Ultra-rapid Warming by an IR Laser Pulse Bo Jin, The University of Tennessee, and Peter Mazur |
| 4:15 | C-1016 | Implications of the Consequences of Laser-induced Ultra-rapid Warming of Mouse Oocytes and Embryos to Vitrification Theory and to the Successful Vitrification of Other Cell Types Peter Mazur, The University of Tennessee |
| 4:30 | C-1017 | Improving Cryoprotectant Tolerance of Zebrafish Oocytes Ali Eroglu, Medical College of Georgia/Georgia Regents University, Edyta Szurek, and De-Qiang Miao |
Plant Transformation Improvements
Moderator: Mindy Fitter, Dow AgroSciences
| 3:30 pm – 5:00 pm | Plant Contributed Paper Session | Regency DEF |
| 3:30 | P-1016 | Improvement of Sunflower Transformation Through the Use of Cotyledon Explants from Mature Seeds Zhifen Zhang, The Ohio State University |
| 3:45 | P-1017 | A Series of DNA Promoters and a Versatile Gene Expression Vector pSiM24 Useful for Transient Assays and Plant Made Products as well as Stable Expression of Foreign Genes in Plants Indu B. Maiti, University of Kentucky, and Dipak Sahoo |
| 4:00 | P-1018 | Optimizing Critical Factors Affecting Agrobacterium Mediated Genetic Transformation of Cassava (Manihot Exculanta) Cultivars Raj Deepika Chauhan, Donald Danforth Plant Science Center, Getu Beyene, and Nigel Taylor |
| 4:15 | P-1019 | Constitutive Expression of Osa-miR528 Alters Plant Development and Enhances Plant Tolerance to Salinity Stress and Nitrogen Starvation in Transgenic Creeping Bentgrass Shuangrong Yuan, Clemson University, Zhigang Li, Dayong Li, Qian Hu, and Hong Luo |
| 4:30 | P-1020 | An Intragenic Approach for the Genetic Improvement of Citrus Manjul Dutt, University of Florida, and Jude Grosser |
| 4:45 | P-1021 | NMR-based Metabolomics Profile Comparison to Distinguish Between Embryogenic and Non-embryogenic Callus Tissue of Sugarcane at the Biochemical Level Kamal Chowdhury, Claflin University, Iqbal Mahmud, Bhawana Shrestha, and Arezue Boroujerdi |
| 5:00 pm – 5:30 pm | SIVB Business Meeting (All Members Are Urged to Attend) Student Award Presentations |
Regency C |
| 5:45 pm – 9:00 pm | An Evening at Old Fort Jackson Admittance by Advance Ticket Holders Only |
Old Fort Jackson |