Daily Program
Abstracts & Posters
Keynote Speaker Keynote Symposia Plant Contributed Papers
Saturday, May 31 Plenary Symposia Animal Posters
Sunday, June 1 Animal Symposia & Workshops Education Posters
Monday, June 2 Plant Symposia & Workshops  Plant Posters
Tuesday, June 3 Animal Contributed Papers Cryobiology Symposia
Wednesday, June 4 Cryobiology Contributed Papers Cryobiology Posters
Late Animal Posters
Late Plant Posters

Sunday, June 1

Daily Program-at-a-Glance

Time Event Location
7:00 am – 5:30 pm Registration Registration Booth
10:00 am – 2:00 pm
5:30 pm – 7:30 pm
Exhibits and Posters Harborside Center
Morning 7:00 am – 12:30 pm  
7:00 am – 8:00 am In Vitro Animal Cell Sciences Program
Committee Meeting
Verelst
7:00 am – 9:00 am Plant Biotechnology Program Committee
Meeting
Percival/Vernon
8:00 am – 10:00 am Climate Change, the Cryosphere and
Cryopreservation
Regency AB
10:00 am – 10:30 am Coffee Break Harborside Center
  SIVB Nominating Committee Meeting Verelst
10:30 am – 12:00 pm Cryo-Biomedical Engineering 1:
Cryobiology Fundamentals
Scarborough 1/2
10:30 am – 12:30 pm Cold Hardiness and Cold Adaptation Regency DEF
  Haploid Technology and the Application
for Plant Breeding and Biotechnology
Regency AB
  Stem Cells in Regenerative
Medicine and Toxicology
Regency C
11:00 am – 12:00 pm SIVB/IAPB/Springer Business Meeting Verelst
Afternoon 12:30 pm – 6:30 pm  
12:30 pm – 1:30 pm Exhibitors/World Forum Refreshment Break Harborside Center
  Cryobiology Editorial Board Meeting Plimsoll
  SIVB Membership Committee Meeting Westbrook
  In Vitro–Plant Editorial Board Meeting Verelst
1:30 pm – 3:00 pm Cell Death Continuum: Its Role in
Cryomedicine
Scarborough 1/2
  Generation and Analysis of Engineered 3D
Tissues–Promising Tools for Advancing
Human Health
Regency C
  Genetic Improvement of Fruit Crops Regency AB
  Mineral Nutrition of In Vitro Plants Regency DEF
  Novel Strategies to Cryoprotection Percival/Vernon
3:10 pm – 5:30 pm 2014 World Forum on Biology Opening Ceremony

3:10 Welcome and Opening Remarks
3:30 Robert H. Lawrence, Jr. Keynote Symposium
4:35 Society for In Vitro Biology Awards Ceremony
4:40 SIVB Fellow and Young Scientist Award Recipients
4:45 SIVB Distinguished Service Award Presentations
5:00 SIVB Lifetime Achievement Award Presentations
Regency AB
5:30 pm – 6:30 pm 2014 World Forum on Biology Opening
Ceremony Reception
Harborside Center
Evening 6:30 pm – 9:30 pm  
6:30 pm – 7:30 pm Poster Session
Odd poster authors will be present
Harborside Center
7:30 pm – 9:30 pm Student Symposium: Effectively
Communicating Science
Percival/Vernon

Sunday, June 1

Program is subject to change
7:00 am – 5:30 pm Registration Registration Booth

Climate Change, the Cryosphere and Cryopreservation
Conveners: Jason Acker, University of Alberta, and Lia H. Campbell, Cell and Tissue Systems, Inc.

8:00 am – 10:00 am Plenary Symposium Regency AB

Climate change is having a dramatic effect on global biodiversity. Cryopreservation is an enabling technologies for international efforts at preserving genetic biodiversity. This session will examine 1. how climate change affects soil biodiversity, and ecosystem processes and services; and 2. how cryopreservation has been used to create genome resource banks for plant and animal cells and tissues.

8:00   Introduction (J. Acker and L. H. Campbell)
8:05 PS-1 Biotic Responses to Climate Change in Antarctic Dry Valleys
Diana Wall, Colorado State University
8:40 PS-2 Coral Cryopreservation Offsetting Climate Change on Reefs
Mary Hagedorn, Smithsonian Institution
9:15 PS-3 Cryopreservation as a Tool for Securing the Future of Plant Biodiversity
Valerie Pence, Cincinnati Zoo and Botanical Garden
9:50   Discussion
10:00 am – 10:30 am Coffee Break Harborside Center

Cold Hardiness and Cold Adaptation

Convener: Maria M. Jenderek, USDA-ARS

10:30 am – 12:30 pm Plant Symposium Regency DEF

Cold hardiness and cold adaptation are closely interconnected. Both phenomena determine the ability of plants grow and survive in low temperatures, affect the geographical distribution for crop or as a part of an ecological system and as a result affect food security and human health. Cold hardiness and cold adaptation also influence plant capacity to endure long-term storage in subzero temperatures, a way of preserving world’s plant genetic resources. The events involve a complex of molecular, biochemical and physiological processes that are not wholly understood. This session will discuss recent advances in determining the nature and function of gene regulation present in signaling cascades, transcriptional control, protection of membranes and proteins, water and ion uptake during cold acclimation as well as the role of carbohydrate metabolism.

10:30   Introduction (M. M. Jenderek)
10:35 P-1 The Role of Secondary Metabolites and COR Proteins in Plant Freezing Tolerance
Dirk Hincha, Max Planck Institute of Molecular Plant Physiology
11:10 P-2 Gene Regulation in Response to Low Temperature
Michael Thomashow, Michigan State University
11:45 P-3 Live/In Planta/Monitoring of Cold-responsive Gene Expressions at Low Temperature
Matsuo Uemura, Iwate University

Cryo-Biomedical Engineering 1: Cryobiology Fundamentals

Convener: Dayong Gao, University of Washington; Co-Convener: Gang Zhao

Researchers and presenters are invited to participate in this session to develop understanding of cryobiology fundamentals and research methodology, and to promote efforts in implementing biophysical and engineering principles to cryopreservation. Dissemination of knowledge by presenting new research results, developments, theories/concepts, and methods in cryobiology research will serve as the foundation for this session.

10:30 C-1 The Use of Engineering Modelling in Designing Cryopreservation Protocols for Articular Cartilage
Janet Elliott, University of Alberta
10:50 C-2 Kinetic Vitrifcation: Basic Thermodynamics, Methods and Devices
Igor I. Katkov, Celltronix
11:10 C-3 Vitrification of Pig Oocytes with Nanoparticle-containing CPAs
Baolin Liu, Institute of Biothermal Science
11:30 C-4 Modulation of Heat and Mass Transfer During Freezing/Thawing
Gang Zhao, University of Science and Technology of China
11:50 C-5 Nanotechnology Enabled Thermal Conductivity Sensor: a Versatile High Resolution Biomedical Device for Quantitative Thermal Property Measurement of Biomaterials
Xin Liang, University of Science and Technology of China

Haploid Technology and the Application for Plant Breeding and Biotechnology

Conveners: Weiguo Liu, Dupont Pioneer, and Todd J. Jones, Dupont Pioneer

10:30 am – 12:30 pm Plant Symposium Regency AB

The ability to regenerate haploid or doubled-haploid (DH) plants is a very useful technology that has been adopted broadly in many crop breeding programs. Pure, homozygous parent lines can be produced in one or two generations compared to three or more years using conventional backcrossing. In maize, DH breeding has become routine and is a vital component of inbred breeding for many companies. In other species, such as Brassica napus, transformation of haploid microspores and the regeneration of double haploid plants are possible. Beyond conventional plant breeding, other applications for haploid technology include genetic linkage research, QTL mapping population development and cytogenetic analysis. This session will explore the various applications of haploid plant production and new methods for DH plant production.

10:30   Introduction (W. Liu and T. J. Jones)
10:35 P-4 Enhanced Methods for Doubled Haploid Production in Triticum aestivum
Tyler Suelter, Heartland Plant Innovations
11:10 P-5 Maize Microspore Embryogenesis
Joe Petolino, Dow AgroSciences
11:45 P-6 Towards a Non-transgenic CENH3-mediated Genome Elimination System
Shamoni Maheshwari, University of California-Davis
12:20   Discussion

Stem Cells in Regenerative Medicine and Toxicology

Conveners: Paul J. Price, Magnolia Ariza-Nieto, Cornell University, Lia H. Campbell, Cell and Tissue Systems, Inc., and Eugene Elmore, University of California-Irvine

10:30 am – 12:30 pm Animal Symposium Regency C

This is an exciting time in the field of stem cells (SC) for the formation of tissue constructs in regenerative medicine and as model systems for invitro toxicology. Regenerative medicine aims to change the course of chronic disease through the use of differentiated multipotent SC to regenerate tired and/or failing organ systems. The goal globally is to improve human health with the use of normal human in vitro models and systems. This session will feature speakers whose findings are state of the art in academia and industry.

10:30   Introduction (P. Price, M. Ariza-Nieto, L. H. Campbell, and E. Elmore)
10:35 A-3 Stem Cells in Regenerative Medicine and Toxicology
Kyle Kolaja, Cellular Dynamics
11:20   Discussion
11:30 A-2 Isolation and Growth of Embryonic and Induced Pluripotent Stem Cells
Demetri D. Spyropoulos, Medical University of South Carolina
12:15   Discussion

Cell Death Continuum: Its Role in Cryomedicine

Convener: Mike Taylor, Cell and Tissue Systems; Co-Convener: John Baust

1:30 pm – 3:00 pm Cryobiology Symposium Scarborough 1/2

Cryomedicine encompasses the application of cryobiology in a wide variety of clinical and health-related sub-disciplines that include biopreservation, hypothermic organ storage and cryoablation of tumors. Underlying all of these applications is the recognition that there is a molecular basis for the stresses imposed by cooling and warming that continues to impact cell survival beyond the immediate point of return to physiological temperature and conditions. This is the so-called cell death continuum involving both necrotic and apoptotic processes. This session will address the impact of the cell death continuum in cryomedicine by considering the role of ischemia-reperfusion injury in hypothermic organ systems, and molecular mechanisms of cell death in both biopreservation and cryoablative surgery.

1:30 C-6 Clinical Impact of the Cell Death Continuum in Hypothermic Organ Systems Manifest as Ischemia/Reperfusion Injury
Mike Taylor, Cell and Tissue Systems
1:55 C-7 The Impact of the Molecular-based Cell Death Continuum on Cryoablation and Biopreservation Outcome
John Baust, CPSI Biotech
2:20 C-8 Molecular Mechanisms of Cell Death Following Cryosurgery and the Impact of Super Critical Nitrogen
Anthony T. Robilotto, CPSI Biotech
2:40 C-9 Monitoring the Effects of Dual Thermal Ablation on Pancreatic Cancer Cell Line PANC-1
Kenneth W. Baumann, Binghamton University, Kristi Snyder, John M. Baust, Robert Van Buskirk, and John G. Baust
2:55   Discussion

Generation and Analysis of Engineered 3D Tissues – Promising Tools for Advancing Human Health

Conveners: Terry Riss, Promega, and John W. Harbell, JHarbell Consulting LLC

1:30 pm – 3:00 pm Animal Symposium Regency C

The rise of tissue engineering/regenerative medicine and a greater understanding of the importance of cell shape, cell-cell interaction, and more physiological cell/tissue exposure have driven the need to develop and evaluate three dimensional (3D) “tissue” constructs. While these constructs can address certain limitations inherent to the monolayer culture, they present their own series of challenges. This session will focus on three main areas. It will provide an overview of the various types of 3D culture model systems being used for research and screening, with the advantages and disadvantages of each. It will also address verification of performance of assays that were originally designed for 2D cultures, including lysis of microtissues and designing appropriate controls, penetration/diffusion of reagents, potential quenching of signals, challenges of imaging “large” structures, etc. Lastly, the session will describe overview of current and future applications of 3D culture technology from commercial suppliers (and others)
for research/testing and clinical applications such as skin replacements.

1:30   Introduction (T. Riss and J. W. Harbell)
1:35 A-4 Generation and Analysis of Engineered 3D Tissues – Promising Tools for Advancing Human Health
Terry Riss, Promega Corporation
2:15 A-5 Commercial 3D Tissue Models: Safety, Product Development, Clinical Applications of Available Products and Future Prospects
Patrick Hayden, MatTek Corporation
2:55   Discussion

Genetic Improvement of Fruit Crops

Conveners: Manjul Dutt, University of Florida, and Yinghui Dan, IALR & Virginia Polytechnic Institute and State University

1:30 pm – 3:00 pm Plant Symposium Regency AB

Genetic improvement of most fruit crops is a slow process due to their biological and reproductive characteristics. Most fruits crops are also propagated asexually and their commercial application is usually restricted to a handful of elite cultivars. These cultivars face several abiotic and biotic challenges as well as low yield. To address these challenges further genetic improvement of the crops requires the advancement of functional and structural genomics, transcriptomics, applied genomics, transformation, and molecular breeding. Our session will focus on recent progress in precision breeding, applied genomics and other genetic improvement technologies for the development of fruit cultivars that can better withstand the numerous challenges facing the industry.

1:30   Introduction (M. Dutt and Y. Dan)
1:35 P-7 Precision Breeding of Grapevine: A Biologically-sound Method for Genetic Improvement
Dennis Gray, University of Florida
1:55 P-8 ‘FasTrack’ – A Revolutionary Approach to Long-generation Cycle Tree Fruit Breeding
Doug Raines, USDA
2:15 P-9 Recombinase-directed Plant Gene Transfer
David Ow, Chinese Academy of Science
2:35 P-10 Genetic Improvement of Stone Fruits Using SNPs
Jayasankar Subramanian, University of Guelph
2:55   Discussion

Mineral Nutrition of In Vitro Plants

Convener: Barbara M. Reed, USDA-ARS, and Valerie Pence, Cincinnati Zoo and Botanical Garden

1:30 pm – 3:00 pm Plant Symposium Regency DEF

Optimal growth of plants in vitro seems to be an elusive goal and there are many approaches to optimizing culture response. Screens of plant growth regulator concentrations are often the first and only step in optimization. Testing well known growth medium formulations is the second most used procedure. These types of studies only touch the surface of the requirements for optimal in vitro response. The mineral nutrients used in plant growth media are often not optimized, resulting in nutrient deficiencies and sometimes toxicity. This session will explore the effects of mineral nutrients on plants as well as on in vitro cultures and some methods used to determine the optimal concentrations of the required mineral nutrients.

1:30   Introduction (B. M. Reed and V. C. Pence)
1:35 P-11 Plant Adaptation to Mineral Nutrient Stresses in the Soil
Leon Kochian, USDA-ARS
2:00 P-12 A Systematic Approach for Optimizing In Vitro Mineral Nutrition in Diverse Red Raspberry Germplasm
Sukayla Poothong, Oregon State University
2:25 P-13 Non-nutrient Factors That Affect Optimal Nutrient Concentrations
Jeffrey Adelberg, Clemson University
2:50   Discussion

Novel Strategies to Cryoprotection

Moderator: Peter Mazur, The University of Tennessee

1:30 pm – 3:00 pm Cryobiology Contributed Paper Session Percival/Vernon
1:30 C-1000 Effect of Hydroxapatite Nanoparticles on Subzero Water Transport of HeLa Cells
Jingru Yi, University of Science and Technology of China, and Gang Zhao
1:45 C-1001 Small Molecule Inhibitors of Ice Recrystallization – From Design to Mechanism of Action and Applications
Robert N. Ben, University of Ottawa, Chantelle Capiccciotti, Anna Balcerzak, Holly Thompson, Adele Hansen, and Tracey Turner
2:00 C-1002 Survivals Approach 100% for Mouse Oocytes Suspended in One-third Concentration of Vitrification Media, Vitrified, and Warmed Ultra-rapidly by an IR Laser Pulse
Bo Jin, The University of Tennessee, F. W. Kleinhans, and Peter Mazur
2:15 C-1003 Cryopreservation of Rat Hepatocytes with Wheat Proteins: Role in Oxidative Stress Protection
Diana A. Averill-Bates, Université du Québec à Montréal, Mélanie Chow-Shi Yée, Mélanie Grondin, Fathey Sarhan, and François Ouellet
2:30 C-1004 Molecular Modelling of the Dynamic and Thermodynamic Characteristics Associated with the Glass Transition of Amorphous Trehalose/Water Mixtures
Lindong Weng, University of North Carolina at Charlotte, and Gloria Elliott
2:45 C-1005 A Novel and Highly Efficient Method for Intracellular Delivery and Accumulation of Trehalose
Alireza Abazarai, Massachusetts General Hospital, Labros Meimetis, Daniel Moore, Steven Hand, Ralph Weissleder, and Mehmet Toner

2014 World Forum on Biology Opening Ceremony

SfC Program Chair: Jason Acker, Canadian Blood Service
SIVB Program Chair: T. Michael Spencer, Monsanto Company

3:10 pm – 5:30 pm Opening Ceremony Regency AB
    Welcome and Opening Remarks:
3:10   Eugene Elmore, President, Society for In Vitro Biology
3:20   Erik Woods, President, Society for Cryobiology
3:30   ROBERT H. LAWRENCE, JR. KEYNOTE SYMPOSIUM
    Introduction (J. Acker and T. M. Spencer)
  KS-1 Technology and Ecology Aspects of Genome Resource Banking
Pierre Comizzoli, Reproductive Physiologist, Smithsonian Conservation Biology Institute
4:20   Questions and Answers
4:35   2014 SOCIETY FOR IN VITRO BIOLOGY AWARDS CEREMONY
Eugene Elmore, President, Society for In Vitro Biology
4:40   2014 Fellow and Young Scientist Award Recipients (Awards to be presented at Section Meetings)

Fellow: Theodore M. Klein, DuPont Pioneer
Young Scientist: Stephanie De Witte-Orr, Wilfrid Laurier University
4:45   2014 Distinguished Service Award Presentations
(Awards to be presented by David D. Songstad):
    Board Members
Lia H. Campbell, Cell & Tissue Systems, Inc.
Theodore M. Klein, DuPont Pioneer
Lucy E. J. Lee, University of the Fraser Valley
Wayne A. Parrott, University of Georgia
William J. Smith
    Program
Barbara M. Reed, USDA/ARS
T. Michael Spencer, Monsanto Company
    Student Affairs
Pamela J. Weathers, Worcester Polytechnic Institute
    2014 Lifetime Achievement Award Presentations:
5:00   (Introduction by Saneteka Shirahata; Acknowledgement by Dr. Barnes to follow)
David W. Barnes, Georgia Gwinett College
5:15   (Introduction by Ray Gillis; Acknowledgement by Dr. Kane to follow)
Michael E. Kane, University of Florida
5:30   Adjourn
5:30 pm – 6:30 pm 2014 WORLD FORUM ON BIOLOGY OPENING CEREMONY RECEPTION Harborside Center
5:30 pm – 7:30 pm Exhibits and Posters Harborside Center
6:30 pm– 7:30 pm Odd Poster Authors will be in attendance Harborside Center

Student Symposium: Effectively Communicating Science

Conveners: Marten Peterson, and Nicole Nole, St. John’s University

7:30 pm – 9:30 pm Education Symposium Percival/Vernon

Good communication is vital to the entire scientific world. Whether discussing data with peers, writing grant applications, or being interviewed by a journalist, information should not go unnoticed because you can’t effectively convey its significance. This interactive symposium is designed to inspire students and young professionals to develop and enhance their communication skills. Speakers will work together, sharing key tips and techniques they have learned throughout their many years of experience. The session will emphasize the challenges of making science compelling for general audiences

7:30   Introduction (M. Peterson and N. Nole)
7:40   Discussion
Speakers:
Wayne Parrott, University of Georgia
Patricia Thomas, University of Georgia


 
WORLD FORUM
ORGANIZERS
  • Jason Acker, SfC Program Chair, Canadian Blood Service
  • T. Michael Spencer, SIVB Program Chair, Monsanto Company
  • Marietta Wheaton Ellis, World Forum Secretariat