Monday, June 2
Daily Program-at-a-Glance
| Time | Event | Location |
|---|---|---|
| 7:00 am – 5:00 pm | Registration | Registration Booth |
| 10:00 am – 3:30 pm | Exhibits and Posters | Harborside Center |
| Morning | 7:00 am – 12:30 pm | |
| 7:00 am – 8:00 am | SIVB Publications Committee Meeting | Verelst |
| 8:00 am – 10:00 am | Stress Tolerance in a Changing Climate | Regency AB |
| 10:00 am – 10:30 am | Coffee Break | Harborside Center |
| SIVB Education Committee Meeting | Verelst | |
| 10:30 am – 12:30 pm | Comparative Reproductive Cryobiology; New Advances and Potential Applications | Scarborough 1/2 |
| Cryo-Biomedical Engineering 2: Technology and Methods in Cryopreservation | Savannah | |
| Natural Products in Human Health | Regency C | |
| Plant Biotechnology Post Doctoral Oral Presentation Competition | Regency DEF | |
| Plant Biotechnology Student Oral Presentation Competition |
Regency AB | |
| Afternoon | 12:30 pm – 6:30 pm | |
| 12:30 pm – 1:30 pm | SIVB Long Range Planning Committee Meeting | Verelst |
| Refreshment Break | Harborside Center | |
| 12:30 pm – 2:00 pm | Student Networking Luncheon: Expert Advice for the Future’s Experts |
Percival/Vernon |
| 1:30 pm – 2:30 pm | Interactive Poster Sessions: | Scarborough 3/4 |
| In Vitro Animal Cell Sciences Student and Post Doctoral Oral Presentation Competition | Regency C | |
| 1:30 pm – 2:45 pm | SfC Peter L. Steponkus Crystal Award Presentations | Savannah |
| 2:30 pm – 3:30 pm | Poster Session Even Poster Authors will be present |
Harborside Center |
| 3:30 pm – 5:00 pm | Advances in Molecular Breeding | Regency AB |
| Comparison of Commercial Micropropagation Production Systems |
Regency DEF | |
| Ice Biophysics: Interactions Between Ice Crystals and Biologicals | Scarborough 1/2 | |
| Nanobiotechnology–Novel Strategies to Target Cellular Behavior and Tumor Development | Regency C | |
| Non-Competitive Student Oral Presentations |
Percival/Vernon | |
| Tissue Biopreservation and Banking | Savannah | |
| 5:00 pm – 6:00 pm | Controlling Contamination Workshop | Regency AB |
| Cryobiology Practices in the Medical Devices Industry |
Savannah | |
| Cryopreservation of Blood and Stem Cells | Percival/Vernon | |
| In Vitro Animal Cell Sciences Contributed Papers |
Regency C | |
| In Vitro Methods for Plant Improvement and Preservation |
Regency DEF | |
| Evening | 6:30 pm – 9:30 pm | |
| 6:00 pm – 7:30 pm | In Vitro Animal Cell Sciences Section Business Meeting |
Regency C |
| Plant Biotechnology Section Business Meeting |
Regency AB | |
| SfC AMG Meeting | Savannah | |
| 7:30 pm – 10:00 pm | World Forum Joint Social | Harborside Center and Riverwalk |
Monday, June 2
| 7:00 am – 6:00 pm | Registration | Registration Booth |
Stress Tolerance in a Changing Climate
Conveners: Marie E. Petracek, Monsanto Company and Lucy E.J. Lee, University of the Fraser Valley
| 8:00 am – 10:00 am | Plenary Symposium | Regency AB |
Plants and animals have evolved distinct stress response strategies to deal with extreme conditions brought about by changing climates. Studies at the cellular level, have demonstrated common and unique strategies for dealing with a variety of stressors, including activation of survival pathways, production of protective molecules and/or initiation of destructive mechanisms that eventually eliminates damaged cells. Whether a cell, be it of plant or animal origins, mounts a protective or destructive stress response depends on the nature and duration of the stress as well as the cell type. This session will provide examples how plants and/or animals cope with stress and the implications of cellular stress responses to survival in a changing climate.
| 8:00 | Introduction (M. E. Petracek and L. E. J. Lee) | |
| 8:20 | PS-4 | Molecular Mechanisms of Cellular Stress Responses: MicroRNA Regulation in Heat Shocked Cells Dick Mosser, University of Guelph |
| 8:50 | PS-5 | Climate Change Mitigation and Adaptation: Continued Innovation in Agriculture is Essential Mike Hall, Monsanto Company |
| 9:20 | PS-6 | Mechanisms of Resilience to Environmental Stressors Within and Among Populations of Killifish Fernando Galvez, Louisiana State University |
| 9:50 | Discussion |
| 10:00 am – 10:30 am | Coffee Break | Harborside Center |
Cryo-Biomedical Engineering 2: Technology and Methods in Cryopreservation
Convener: Gang Zhao, University of Science and Technology China; Co-Convener: Dayong Gao, University of Washington
| 10:30 am – 12:30 pm | Cryobiology Symposium | Savannah |
This session focuses on the new and advanced engineering methods, instruments, BioMEMS/microfluidic chips, and novel technology for the cryopreservation and its applications.
| 10:30 | C-10 | Development of Advanced Technology and Methods for Cryopreservation: Seattle Experience Dayong Gao, University of Washington |
| 10:50 | C-11 | High-Speed Video Imaging of Ice Crystal Formation in Cells, Tissues and Droplets Jens O. M. Karlsson, Villanova University |
| 11:10 | C-12 | Microfluidic Processing of Cryopreserved Blood Adam Higgins, Oregon State University |
| 11:30 | C-13 | MEMS Coulter Counters for Dynamic Impedance Measurement of Time Sensitive Cells James D. Benson, North Illinois University |
| 11:50 | C-14 | Application of Hydrogel in Cryopreservation as a Novel Cryoprotectant, Encapsulation and Single-cell-based Functional Sensing Material Zhiquan Shu, University of Washington |
Comparative Reproductive Cryobiology; New Advances and Potential Applications
Convener: Yuksel Agca, University of Missouri; Co-convener: Ali Eroglu, Medical College of Georgia, Georgia Regents University
| 10:30 am – 12:30 pm | Cryobiology Symposium | Scarborough 1/2 |
This session will cover germplasm cryobiology and cryobanking of laboratory, agricultural, wild life animals and human. The audience will receive comprehensive general information and research findings about the current status of representative species at the end of the session.
| 10:30 | C-15 | Comparative Cryobiology of Germplasms in Non-traditional Species Pierre Comizzoli, Smithsonian Conservation Biology Institute, National Zoological Park |
| 10:54 | C-16 | Potential Applications of Microfluidic Methods to Sort Fresh and Frozen Sperm Utkan Demirci, Harvard Medical School |
| 11:18 | C-17 | Human Oocyte Cryopreservation: Current Status and Outlook Ali Eroglu, Medical College of Georgia, Georgia Regents University |
| 11:42 | C-18 | The First Bench Top Device for Producing Clean Liquid Air (Clair): A Breakthrough in Vitrification with Open Systems Amir Arav, FertileSafe Ltd. |
| 12:06 | C-19 | Zebrafish Cryobiology: Sperm, Oocytes, Embryos and Stem Cells Mary Hagedorn, Smithsonian Institution |
Natural Products in Human Health
Conveners: Brad L. Upham, Michigan State University, and Laura Schramm, St. John’s University
| 10:30 am – 12:30 pm | Animal Symposium | Regency C |
Natural products are chemical compounds produced by living organisms that possess biological activity with pharmacological properties benefiting human health. Medicine practiced by traditional cultures and ancient civilizations heavily relied on local flora and fauna. In recent years there has been a very enthusiastic resurgence of interest in the value of natural products in the prevention and cures of human diseases. Natural products can be isolated from microbial and multicellular flora and fauna of terrestrial and marine environment, or ingested along with dietary foods. Natural products from dietary foods and nutritional supplements are often referred to as nutraceuticals, and are known to benefit long-term human health if consumed at reasonable levels, albeit at levels that lack acute biological effects, over long periods. However, like all compounds with high biological activities, toxic properties must also be determined to establish appropriate doses for beneficial effects. In this session, evidence on the benefits of select natural products on human health and the role of in vitro systems in assessing the benefits and toxicities in human health will be covered.
| 10:30 | Introduction (B. L. Upham and L. Schramm) | |
| 10:35 | A-6 | Bioactives from Natural Products: What Works, What Doesn’t, and What Comes Next Mary Ann Lila, North Carolina State University |
| 11:10 | A-7 | In Vitro Assays of Intercellular Communication as a Biomarker to Determine Chemopreventive Activity of Natural Products Brad L. Upham, Michigan State University |
| 11:45 | A-8 | Pancreatic Cancer and Marine Natural Products Esther A. Guzmán, Harbor Branch Oceanographic Institute at FAU |
Plant Biotechnology Post Doctoral Presentation Competition
Moderator: Harold N. Trick, Kansas State University
Panel of Plant Biotechnology Experts Evaluating the Contestants: Yinghui Dan, IALR & Virginia Polytechnic Institute and State University, Sairam Rudrabhatla, Penn State Harrisburg, and Jyoti Rout, Dow Agro Sciences
| 10:30 am – 12:30 pm | Plant Contributed Paper Competition | Regency DEF |
To support the Society’s vision to encourage education and scientific informational exchange and to recognize outstanding post docs, the Plant Biotechnology Section is pleased to offer a Post Doctoral Oral Presentation Competition. Finalist were selected based on the quality of their abstracts. A panel of judges will evaluate the presentations. Criteria for this evaluation will be based on experimental design, data analysis, proper interpretation of the results, originality of the study, technical difficulty, appearance and ability of the post doc present the work and to respond to questions. Winners will be presented with a certificate and a cash award at the Plant Biotechnology Section’s Business meeting.
| 10:30 | P-1006 | TALEN Mediated Targeted Mutagenesis in Sugarcane Je Hyeong Jung, University of Florida, and F. Altpeter |
| 10:50 | P-1007 | TIR1-like Auxin-receptors Are Involved on the Regulation of Plum Fruit Development Islam El-Sharkawy, University of Guelph, Sherif Sherif, and Jayasankar Subramanian |
| 11:10 | P-1008 | Artificial microRNA as an Alternative Method to Improve the Resistance to Soybean Cyst Nematode Bin Tian, Kansas State University, Jiarui Li, Thomas R. Oakley, Timothy C. Todd, and Harold N. Trick |
| 11:30 | P-1009 | Metabolic Engineering of Orange Fruit for the Development of Novel Blood and Cara Cara like Citrus Varieties Katsuri Dasgupta, UC Davis/ USDA-ARS-WRRC, Roger Thilmony, and James G. Thomson |
| 11:50 | P-1010 | Genetic Instatility of Long-term Micropropagated Mature Pistachio Hulya Akdemir, Gebze Institute of Technology, V. Süzerer, E. Tilkat, A. Onay, and Y. Ozden Çiftçi |
| 12:10 | P-1011 | Overexpression of a Soybean Salicylic Acid Methyltransferase Gene Confers Resistance to Soybean Cyst Nematode Jingyu Lin, University of Tennessee, Mitra Mazarei, Nan Zhao, Mary Rudis, Vincent R. Pantalone, Prakash R. Arelli, Feng Chen, and Charles N. Stewart |
Plant Biotechnology Student Oral Presentation Competition
Moderator: Cecilia Zapata, Driscoll’s
Panel of Plant Biotechnology Experts Evaluating the Contestants: Manjul Dutt, University of Florida, Ian S. Curtis, Chromatin Inc., and Randall Niedz, USDA/ARS
| 10:30 am – 12:30 pm | Plant Contributed Paper Competition | Regency AB |
To support the Society’s vision to encourage education and scientific informational exchange and recognize outstanding students, the Plant Biotechnology Section is pleased to announce the 2014 Student Oral Presentation Competition. Student candidates wishing to enter submitted a copy of their title and abstract to Dr. Cecilia Zapata. Only those abstracts formally submitted to Dr. Zapata were considered. Student competition finalists were selected based on the quality of the abstracts. A panel of judges will evaluate the presentations at the meeting. Criteria are experimental design, data analysis, proper interpretation of the results, originality of the study, technical difficulty, appearance and ability of the student to explain it. Winners will be presented with a certificate and a cash award at the meeting.
| 10:30 | P-1000 | Isolation and Evaluation of New Agrobacterium Strains for Transformation of Soybean and Sunflower Kyle A. Benzle, The Ohio State University, Dee Marty, Leah McHale, Brad Goodner, Kim Finer, Christopher Taylor, and John Finer |
| 10:50 | P-1001 | Optimizing In Vitro Mineral Nutrition and Plant Density Increases Greenhouse Growth Rate of Turmeric Rabia El-Hawaz, Clemson University, Dara Park, William C. Bridges, and Jeffrey Adelberg |
| 11:10 | P-1002 | RNAi Mediated Silencing of Endogenous Wheat Genes elF4E2 and elF4G Induces Resistance to Potyviruses Wheat Streak Mosaic Virus and Tirticum Mosaic Virus Jessica L. Rupp, Kansas State University, Luisa Cruz, John P. Fellers, and Harold N. Trick |
| 11:30 | P-1003 | Development of Switchgrass (Panicum virgatum L.) Transformable Cell Suspension Culture and a Screening System for Rapid Assessment of Cell Wall Genes for Improved Biomass for Biofuels Jonathan D. Willis, University of Tennessee, Lindsey Kline, Sara Allen, Priyanka Bhattacharya, A. Grace Collins, Joshua N. Grant, Garrett Montgomery, Nicole Labbe, and C. Neal Stewart |
| 11:50 | P-1004 | A Leading Intron of a Soybean Elongation Factor 1A Gene Interacts with Soybean Promoter Elements to Regulate Gene Expression Ning Zhang, The Ohio State University, Leah K. McHale, and John J. Finer |
| 12:10 | P-1005 | Insulator Mediated Transgene Performance in Sugarcane Yang Zhao, University of Florida, Jae Yoon Kim, Chunyang Fan, Wenjin Yu, Shujie Dong, and Fredy Altpeter |
Student Networking Luncheon: Expert Advice for the Future’s Experts
Conveners: Marten Peterson, and Nicole Nole, St. John’s University
| 12:30 pm – 2:00 pm | Education Workshop | Percival/Vernon |
Networking in an essential part of a scientist’s career and is particularly critical during the transition from student to professional. The World Forum on Biology brings together a diverse group of experts from across the globe, many of whom are interested in forging relationships with the students who will soon become their colleagues. This luncheon provides a great opportunity to talk with fellow students, young researches, and renowned professionals, in order to enhance networking skills and expand personal networks. Join us for an informal lunch with senior members and invited guests who wish to share their experiences, discuss trends in their respective fields, as well as pass along some valuable advice they’ve collected throughout their careers. Participants are free to come and go as they wish and are encouraged to move between tables to join in different conversations.
| 12:30 | Introduction (M. Peterson and N. Nole) | |
| 12:35 | Lunch |
In Vitro Animal Cell Sciences Student and Post Doctoral Oral Presentation Competition
Moderator: Addy Alt-Holland, Tufts University
Panel of In Vitro Animal Cell Sciences Experts Evaluating the Contestants: Michael J. Fay, Midwestern University, Magnolia Ariza-Nieto, Cornell University, Brad L. Upham, Michigan State University, John W. Harbell, JHarbell Consulting, LLC, Barbara Doonan, New York Medical College, Kathryn M. Houk, Tufts University Hirsch Health Sciences Library, and Addy Alt-Holland, Tufts University
| 1:30 pm – 2:30 pm | Animal Contributed Paper Competition | Regency C |
To support the Society’s vision to encourage education and scientific informational exchange and recognize outstanding students and post docs, the In Vitro Animal Cell Sciences section is pleased to announce the 2014 In Vitro Animal Cell Science Research Oral Presentation Competitions for both Students and Post Docs. Students and Post Docs wishing to participate in this competition submitted a copy of their title and abstract (as submitted to SIVB) and abstract tracking number to Dr. Addy Alt-Holland. Only those abstracts formally submitted to both SIVB and Dr. Alt-Holland were considered. Finalists were selected based on the quality of the abstracts. A panel of judges will evaluate the presentations at the meeting. Criteria are experimental design, data analysis, proper interpretation of the results, originality of the study, technical difficulty, appearance of the presentation and ability of the finalist to respond to questions. Winners will be presented with a certificate and a cash award at the meeting.
| 1:30 | A-1000 | Development and Characterization of a Honey Bee (Apis mellifera) Cell Line Michael Goblirsch, University of Minnesota, Marla Spivak, and Timothy Kurtti |
| 1:45 | A-1001 | Abstract has been withdrawn from this session |
| 2:00 | A-1002 | Regulation of RNA Polymerase III Transcription by Daidzein in MCF-7 Cells Jana Koo, St. John’s University, and Nicole Nole |
| 2:15 | A-1003 | Profiles of Secretory Mature hsa-miR-22-3p and DNMT1 Transcript Abundance During Hepatogenic Differentiation of Mesenchymal Stem Cells (MSCs) Chunyan Wu, Cornell University, Joshua B. Alley, Sanjay A. Samy, Laura Fitzgerald, Andrew C. Trecartin, Michael L. Shuler, and Magnolia Ariza-NIeto |
Biotechnological Approaches to Plant Stress Tolerance and Development
Moderator: Hong Luo, Clemson University
| 1:30 pm – 2:30 pm | Plant Interactive Poster Session | Scarborough 3/4 |
| P-2000 | Enhancing Halotolerance in the Cyanobacterium Fremyella diplosiphon Through Biotechnological Approaches Benham Tabatabai, Morgan State University, Sigamani AnithaChristy, Beronda Montgomery, and Viji Sitther |
| P-2001 | Targeting Methylglyoxal Detoxification Pathway: An Efficient Approach to Improve Multiple Abiotic Stress Tolerance in Leguminous Crop Plant Preeti Singh, Jawaharlal Nehru University, Deepak Kumar, Mohd. Aslam Yusuf, and Neera Bhalla Sarin |
| P-2002 | Evaluation of a Vitis-derived Reporter Gene System for Precision Breeding of Cold-Hardy Grapevine Cultivars Raju Kandel, University of Wyoming, Manjul Dutt, Jude Grosser, Dennis Gray, Zhijian Li, Viji Sitther, Daniel Bergey, and Sadanand Dhekney |
| P-2003 | The Effect of Silver Ions and Silver Nanoparticles on Plant Development Jiangong Liang, Clemson University, Zhigang Li, Qian Hu, and Hong Luo |
| P-2004 | Heterologous Expression of the Rice MicroRNA395h in Nicotiana tabacum Impairs the Sulfate Homeostasis Ning Yuan, Clemson University, Zhigang Li, Dayong Li, and Hong Luo |
Genetic Transformation, Metabolic Engineering, and Secondary Metabolism
Moderator: Manjul Dutt, University of Florida
| 1:30 pm – 2:30 pm | Plant Interactive Poster Session | Scarborough 3/4 |
| P-2005 | Regulatory Mechanisms of Sesquiterpenoid Biosynthesis Revealed by Gene Expression and Metabolite Profiling in Valeriana officinalis Vincent Ricigliano, St. Johns University, and Dianella Howarth |
| P-2006 | Identification and Characterization of Genes Involved in the Fruit Color Development of European Plums (Prunus domestica L.) Dinesh Kumar Selvaraj, University of Guelph, Sherif Sherif, Krishnaraj Tiwari, Gopinadhan Paliyath, and Jayasankar Subramanian |
| P-2007 | A Self-regulating MYB10 Transgene from Apple Confers Ectopic Anthocyanin Accumulation to Flowers but not Leaves in Petunia Murray R. Boase, The New Zealand Institute for Plant and Food Research Limited, Cyril Brendolise, David Lewis, Lei Wang, Hanh Ngo, Kathy Schwinn, Kevin Davies, Richard Espley, and Nick Albert |
| P-2008 | Metabolic Redesign of Vitamin E Biosynthesis in Brassica juncea for Human Health and Stress Alleviation in Plants Deepak Kumar, Jawaharlal Nehru University, Mohd. Aslam Yusuf, Preeti Singh, Meryam Sardar, and Neera Bhalla Sarin |
| P-2009 | Novel In-vitro Biosynthesis of Lignin Vikrant Sharma, Meadow Park Summa MS |
| P-2010 | Developmental Regulation of the VvmybA1 Transcriptional Factor Gene of Grapevine (Vitis vinifera L.) to Produce Reporter Gene Expression Free Transgenic Citrus Leonardo Soriano, Universidade de Sao Paulo, Manjul Dutt, Beatriz Madalena Januzzi Mendes, and Jude William Grosser |
Peter L. Steponkus Crystal Award Presentations
Moderator: James Benson, Northern Illinois University
| 1:30 pm – 2:45 pm | Cryobiology Contributed Paper Session | Savannah |
| 1:30 | C-1006 | Exploring the Improvement of Human Cell Cryopreservation Tim Morris, Loughborough University, Karen Coopman, Chris Hewett, and Andrew Picken |
| 1:48 | C-1007 | The Design of Small Molecule Ice Recrystalization Inhibitors for Use as Cryopreservatives Jennie Briard, University of Ottawa, Robert Ben, Melody Afagh, and Emily Gardiner |
| 2:06 | C-1008 | Compositional and Functional Analyses of Glyco-sylphosphatidylinositol-anchored Protein (GPI-AP) During Plant Cold Acclimation Daisuke Takahashi, Iwate University |
| 2:24 | C-1009 | Cryopreservation of Monkey Mesenchymal Stem Cells Inside Alginate 3D Micro-Spheres After a High Voltage Encapsulation Oleksandr Gryshkov, Leibniz University Hannover, Denys Pogozhykh, Nicola Hofmann, Thomas Mueller, and Birgit Glasmacher |
| 2:30 pm – 3:30 pm | Even Poster Authors will be present | Harborside Center |
Advances in Molecular Breeding
Convener: Wayne A. Parrott, University of Georgia
| 3:30 pm – 5:00 pm | Plant Symposium | Regency AB |
Advances in technology are enabling dramatic advances in plant breeding methodology. High-throughput genotyping was enabled by the integrated use of robotics, bioinformatics and DNA analysis. In turn, high-throughput genotype makes it possible for a breeder to efficiently and cost effectively monitor a far larger number of traits and screen much larger plant populations than was possible a just few years ago. Transgenic technology is also replacing the old male sterility systems that were in place for hybrid seed production. The new hybrid seed production systems are easier to develop and more economical and easier to implement. Collectively, these technologies are ensuring that new varieties and hybrids reach the market in record time.
| 3:30 | Introduction (W. Parrott) | |
| 3:35 | P-14 | Evolution of Molecular Breeding at Monsanto Jeff Woessner, Monsanto Company |
| 4:00 | P-15 | Science of Seed Production Technology: A Transgene Process for Hybrid Seed Production Mark Cigan, DuPont Pioneer |
| 4:25 | P-15 | TBD |
| 4:50 | Discussion |
Comparison of Commercial Micropropagation Production Systems
Conveners: Michael E. Kane, University of Florida, and Ray Gillis, Oglesby Plants International, Inc
| 3:30 pm – 5:00 pm | Plant Symposium | Regency DEF |
Since the commercial application of in vitro propagation became a reality in the early 1960s, micropropagation has become a global industry. However, the industry continually faces economic challenges related to high production costs, especially labor. Depending on the crops, laboratories have adapted different technologies to increase production efficiencies and maintain consistent plant quality. These include incorporating robotics, bioreactors, low energy lighting, contamination control and environmentally-sound automated greenhouse plug production into their production model. During this session commercial laboratory production managers will describe their specific production facilities and how they have addressed their production challenges. An interactive group discussion will follow during which participants will have the opportunity to share their views regarding the current and future needs required to advance commercial micropropagation.
| 3:30 | Symposium Overview (M. E. Kane) | |
| 3:35 | P-18 | Micropropagation Production at Oglesby Plants International, Inc. Ray Gillis, Oglesby Plants International, Inc. |
| 3:55 | P-19 | Micropropagation Production at Microplant Nurseries Gayle Suttle, Microplant Nurseries, Inc. |
| 4:15 | P-20 | How to Become a Market Leader in Tissue Culture John Bijl, Vitro Plus |
| 4:35 | Strategies to Enhance Micropropagation Production Efficiency – Group Discussion Michael Kane, University of Florida, and Ray Gillis, Oglesby Plants International, Inc. |
Ice Biophysics: Interactions Between Ice Crystals and Biologicals
Convener: Jens O. M. Karlsson, Villanova University
| 3:30 pm – 5:00 pm | Cryobiology Symposium | Scarborough 1-2 |
Speakers will offer various perspectives on the ways in which ice and biological materials (biomolecules, cells, or tissue) interact, including the effects of biologicals on the nucleation and growth of ice crystals, and vice versa, the biological sequelae of ice formation.
| 3:30 | C-24 | Ice Binding Proteins and Their Interactions with Ice Crystals Ido Braslavsky, The Hebrew University of Jerusalem |
| 4:00 | C-25 | The Enigmatic Role of Intercellular Junction Structures in Tissue Freezing Jens O. M. Karlsson, Villanova University |
| 4:30 | C-26 | Biological Cells, Ice and Bubbles John McGrath, Central Michigan University |
Non-competitive Student Oral Presentations
Moderator: Nicole Nole, St. John’s University, and Jana Koo, St. John’s University
| 3:30 pm – 5:00 pm | Education Workshop | Percival/Vernon |
Public speaking and presenting research are essential skills to members of the scientific community. This session provides an opportunity to practice giving an oral presentation to students whose abstracts were not chosen for a competitive session. All students, even those that did not submit an abstract to the oral competition, are welcome to apply. The speaker will receive constructive criticism from other members and students of SIVB by using the same judging rubric from the competitive session in a friendly, relaxed environment.
| 3:30 | Introduction (N. Nole and J. S. Koo) | |
| 3:35 | P-2008 | Metabolic Redesign of Vitamin E Biosynthesis in Brassica juncea for Human Health and Stress Alleviation in Plants Deepak Kumar, Jawaharlal Nehru University, Mohd. Aslam Yusuf, Preeti Singh, Meryam Sardar, and Neera Bhalla Sarin |
| 3:55 | P-2038 | Wheat Gene Expression Differences Induced by Six Puccinia triticina Races Kerri Neugebauer, Kansas State University, Myron Bruce, and John Fellers |
| 4:15 | P-2005 | Regulatory Mechanisms of Sesquiterpenoid Biosynthesis Revealed by Gene Expression and Metabolite Profiling in Valeriana officinalis Vincent Ricigliano, St. Johns University, and Dianella Howarth |
| 4:35 | P-2010 | Developmental Regulation of the VvmybA1 Transcriptional Factor Gene of Grapevine (Vitis vinifera L.) to Produce Reporter Gene Expression Free Transgenic Citrus Leonardo Soriano, Universidade de Sao Paulo, Manjul Dutt, Beatriz Madalena Januzzi Mendes, and Jude William Grosser |
| 4:55 | Discussion |
Nanobiotechnology – Novel Strategies to Target Cellular Behavior and Tumor Development
Conveners: Addy Alt-Holland, Tufts University and Michael K. Dame, University of Michigan Medical School
| 3:30 pm – 5:00 pm | Animal Symposium | Regency C |
Nanobiotechnology is a newly emerging interdisciplinary research area at the interface of nanotechnology and biology. This session will explore the remarkable potential and use of nanobioparticles, structures of 1-100nm in diameter, which largely due to their high surface area are imbued with exceptional physicochemical characteristics. Their action is examined through in vitro cell culture systems and assays to design, predict and validate function – from a programmable cellular microenvironment to the precise targeting of aberrant cell growth. This unique technical approach to biology enables researchers to address medical and biological questions with a new set of tools. In this symposium, Dr. Tan’s lecture will focus on the synthesis and evaluation of novel nanocarriers for the delivery of molecularly targeted therapeutics that can actively distinguish tumor cells from their normal counterparts thus improving the safety and efficacy of anticancer agents. Dr. Wang’s lecture will focus on the use of nanobioparticles, which can be genetically and chemically modified, for the generation of three-dimensional programmable platforms to probe cellular activities, such as cell adhesion, spreading, migration, and differentiation.
| 3:30 | Introduction (A. Alt-Holland and M. Dame) | |
| 3:35 | A-9 | Micellar Nanocarriers for Improved Cancer Therapy Chalet Tan, Mercer University |
| 4:15 | A-10 | A Nanobiological Route for the Isolation of Cancer Stem Cells Qian Wang, University of South Carolina |
| 4:50 | Discussion |
Tissue Biopreservation and Banking
Convener: Jelena Holovati, University of Alberta
| 3:30 pm – 5:00 pm | Cryobiology Symposium | Savannah |
This session provides a forum for disseminating information to scientists and clinicians involved in the preservation research, clinical banking and transplantation of tissues. Several different biopreservation approaches will be discussed, including hypothermic storage, cryopreservation and vitrification of tissue allografts for transplant, such as heart valves, skin and cartilage.
| 3:30 | C-20 | Dry Preservation of Decelluarized Heart Valve Tissues Willem Wolkers, Leibniz Universitaet Hannover |
| 3:50 | C-21 | Tissue Vitrification Kelvin Brockbank, Cell and Tissue Systems Inc. |
| 4:10 | C-22 | Clinical Banking of Amniotic Membrane Allografts Jelena Holovati, University of Alberta |
| 4:30 | C-23 | The Next Frontier: Vascularized Tissues Greg Fahy, 21st Century Medicine, Inc. |
Controlling Contamination Workshop
Convener: Mary Welter, Dow AgroSciences
| 5:00 pm – 6:00 pm | Plant Workshop | Regency AB |
Bacterial and fungal contamination are an ongoing threat to plant tissue cultures in the laboratory. Contamination is not only responsible for loss of cultures but if left undetected can affect the results of experimental studies. As there are multiple paths for which contamination can be introduced into the laboratory (i.e. initial explant, lab or culture room environment, improperly prepared media or downstream manipulations), multiple strategies need to be implemented to reduce or eliminate contamination. This workshop will provide some practical strategies for initiating and maintaining clean cultures.
| 5:00 | Introduction (M. Welter) | |
| 5:05 | P-21 | Considerations for Contamination Cleanup in Plant Tissue Culture David S. Hart, PhytoTechnology Laboratories |
| 5:30 | P-22 | Best Practices in Tissue Culture for the Control of Biological Contamination Yvonne Reid, ATCC |
| 5:55 | Discussion |
Cryopreservation of Blood and Stem Cells
Moderator: Johan Lagerberg, Sanquin Research
| 5:00 | C-1010 | Author is unable to attend |
| 5:15 | C-1011 | Low Temperature Cell Pausing: An Alternative Short-term Preservation Method for Use in Cell Therapies Natalie Robinson, Loughborough University, Andrew Picken, and Karen Coopman |
| 5:30 | C-1018 | Stabilizing Effect of Liposomes on Rat Red Blood Cell Membrane Quality Is Treatment-time Dependent Luciana da Silveira Cavalcante, University of Alberta and Canadian Blood Services, Jason P. Acker and Jelena L. Holovati |
| 5:45 | C-1012 | Viability and Differentiation of Human Blood and Marrow Cells Cryopreserved Since 1972, and the Homing Effects of Cryopreserved Cells Sajio Sumida, Japan Society for Low Temperature Medicine, Taeko Kitamura, Noboru Motomura, Aya Saito, Evan Hagen, Erick Woods, and Arthur Rowe |
Cryobiology Practices in the Medical Devices Industry
Convener: Jorge Jimenez, Cook Medical
| 5:00 pm – 6:00 pm | Cryobiology Symposium | Savannah |
With the proliferation of cell- and tissue-based therapies and assisted reproduction technologies in mainstream clinical applications, a commercial need has arisen for medical devices to assist in the low temperature storage beyond the capabilities of the hospital laboratory. This brings along a new set of design challenges, as medical device manufacturers attempt to develop a new standard of practice to be used in a clinical setting. This session will highlight some of the work related to cryopreservation and cryobiology done by the medical devices industry in the interest of the development of a device.
| 5:00 | C-27 | Evaluation of Closed System Medical Device for Low Volume Storage for Clinical Studies Involving Regulatory T Cells Evonne Fearnot, Cook General Biotechnology, LLC |
| 5:20 | C-28 | Frozen Storage and Transport of Cell Therapies: Developing a Solution to a Common Challenge Dominic Clarke, Charter Medical, Inc. |
| 5:40 | C-29 | The CryoPen as an Excellent Solution for Low to Middle Income Countries (LMIC) in Cancer Prevention and Treatment Mike Haas, Cryopen, Inc. |
In Vitro Animal Cell Sciences Contributed Paper Session
Moderator: Addy Alt-Holland, Tufts University
| 5:00 pm – 6:00 pm | Animal Contributed Paper Session | Regency C |
| 5:00 | A-1004 | The Role of Viral DsRNA and Host Scavenger Receptors in the Innate Antiviral Response in Fish Stephanie DeWitte-Orr, Wilfrid Laurier University, Amal Aloufi, Jeremy Weleff and Adam Soares |
| 5:15 | A-1005 | Differential Regulation of RNA Polymerase III Transcription by EGCG Nicole Nole, St. John’s University, Jana Koo, Joby Jacob, and Laura Schramm |
| 5:30 | A-1006 | Influence of the Extracellular Matrix on the Survival of Cryopreserved Adherent Cells Lia Campbell, Cell and Tissue Systems, Inc., and Kelvin Brockbank |
| 5:45 | A-1007 | Delineating the Metabolic Profiles of Skin Cancer Cells and Stromal Fibroblasts In Vitro Addy Alt-Holland, Tufts University School of Dental Medicine,Shawheen Saffari, Ronney Tay, John Nolan, and James Baleja |
In Vitro Methods for Plant Improvement and Preservation
Moderator: Jeffrey Beringer, Dow AgroSciences
| 5:00 pm – 6:00 pm | Plant Contributed Paper Session | Regency DEF |
| 5:00 | P-1012 | Twenty Five Years of Innovations on Date Palm Micropropagation Suhersan Chellan, Kuwait Institute for Science Research, Jibi Sudhersan, and Jawad Ashkanani |
| 5:15 | P-1013 | Development and Comparison of Two Different PVS2-based Procedures for Cryopreservation of Sugarcane (Saccharum spp.) Germplasm Ergun Kaya, Gebze Institute of Technology, Fernanda Souza, Leonardo Rodriques, Alfredo Alves, Elif Aylin Ozudogru, Clarissa Maroon-Lango, and David Ellis |
| 5:30 | P-1014 | In Vitro Mutagenesis and Selection of Fusarium Sacchari-tolerant Sugarcane Plants for Biological Control of Eldana saccharina Tendekai Mahlanza, South African Sugcarcane Research Institute, Stuart Rutherford, Sandy Snyman, and Paula Watt |
| 5:45 | P-1015 | Direct Somatic Embryogenesis and Shoot Organogenesis from Apical and Lateral Meristems of Soft Taro (Colocasia esculenta var. esculenta) Virendra M. Verma, College of Micronesia |