Sunday, June 16

Daily Program-at-a-Glance

Time	Event	Room
7:00 am – 5:30 pm	Registration	Ballroom Foyer
10:00 am – 2:00 pm 5:30 pm – 7:30 pm	Exhibits and Posters	Ballroom A
Morning	7:00 am – 12:30 pm
7:00 am – 8:00 pm	In Vitro Animal Cell Sciences Program Committee Meeting	Room 554A
7:00 am – 9:00 am	Plant Biotechnology Program Committee Meeting	Room 556A/B
8:00 am – 10:00 am	History of Biotechnology	Ballroom B
10:00 am – 10:30 am	Coffee Break	Ballroom A
10:00 am – 10:30 am	Nominating Committee Meeting	Room 554A
10:30 am – 12:30 pm	Advances in Transformation Technology	Ballroom B
10:30 am – 12:30 pm	In Vitro Culture and Micropropagation	Ballroom D
10:30 am – 12:30 pm	The Human Microbiome: What Is Known and What Is Next	Ballroom C
11:00 am – 12:00 pm	SIVB/IAPB/Springer Business Meeting	Room 554B
Afternoon	12:30 pm – 5:30 pm
12:30 pm – 1:30 pm	Exhibitors/SIVB Refreshment Break	Ballroom A
12:30 pm – 1:30 pm	Long Range Planning Committee Meeting	Room 554A
12:30 pm – 1:30 pm	In Vitro – Plant Editorial Board Meeting	Room 554B
1:30 pm – 3:00 pm	Forage and Turf Improvement	Ballroom D
1:30 pm – 3:00 pm	New Strategies for Specialty Crops	Ballroom B
1:30 pm – 3:00 pm	Pathogen-host Interactions and Endocrine Disruptors in Human and Fish	Ballroom C
	2013 In Vitro Biology Meeting Opening Ceremony	Ballroom B
3:15	Welcome and Opening Remarks:
3:25	Robert H. Lawrence, Jr. Keynote Symposium
4:30	2013 Society for In Vitro Biology Awards Ceremony
4:35	2013 Fellow and Young Scientist Award Recipients
4:40	2013 Distinguished Service Award Presentations
5:00	2013 Lifetime Achievement Award Presentations
Evening	5:30 pm – 9:30 pm
5:30 pm – 6:30 pm	Opening Ceremony Reception	Ballroom A
6:30 pm – 7:30 pm	Poster Session Odd Poster Authors will be present	Ballroom A
7:30 pm – 9:30 pm	Student Networking Symposium: Strategies for Remaining Current in Your Field	Room 556A/B

Sunday, June 16

History of Biotechnology

Conveners: Fredy Altpeter, University of Florida, and J. Denry Sato, Manzanar Project Foundation

8:00 am – 10:00 pm

Plenary Session

Ballroom B

This plenary session will focus on technological innovations and the biotech products derived from them. Dr. Ted Klein (DuPont) will reflect on the pioneering work of early plant biotechnologists, which arose from advances in plant physiology, tissue culture, molecular genetics and genetic transformation. Their intellectual and experimental advances have led to the creation and large-scale cultivation of high-yielding transgenic crops, making biotech crops the fastest adopted crop technology in recent history. Dr. Keiichi Itakura (Beckman Research Institute of the City of Hope) will discuss advances in the chemical synthesis of DNA and the synthesis, cloning and expression of cDNAs encoding human somatostatin and human insulin. Recombinant human insulin and recombinant human growth hormone, produced in collaboration between the City of Hope and Genentech, were the first genetically engineered biological drugs approved for use in humans, and they paved the way for the current explosion in recombinant biological drugs.

8:00	PS-1	Adventures in Plant Biotechnology Theodore M. Klein, DuPont Pioneer
9:00	PS-2	The Beginnings of Biology *Keiichi Itakura*, Beckman Research Institute of the City of Hope

10:00 am – 10:30 am

Coffee Break

Ballroom A

Advances in Transformation Technology

Conveners: Xudong Ye, Monsanto Company, and J. Pon Samuel, Dow AgroSciences, LLC

10:30 am – 12:30 pm

Plant Symposium

Ballroom B

Significant strides have been made in gene-delivery and expression systems in plants, resulting in efficacious transgenic traits and improved biotech products. The progress made in gene delivery systems including direct DNA uptake, particle bombardment and Agrobacterium-mediated plant transformation has helped the industry and academia realize the fruits of the modern biotechnology. They are powerful tools which are equally important to basic research and for biotechnological applications. Transgenic traits have been integrated into many agronomically important crops including corn, rice, soybean, cotton, canola etc. and commercialized since 1995. While plant transformation has no longer been a hurdle for most crop species, transgene quality such as single copy and backbone-free event frequency, and transformation efficiency improvement through process automation have been addressed in recent years. This session will highlight recent advances in gene delivery, molecular transformation improvement, and transformation process automation.

10:30		Introduction (X. Ye and J. P. Samuel)
10:35	P-1	Automating Plant Transformation T. Michael Spencer, Monsanto Company
11:00	P-2	Molecular Approaches Improve Crop Transformation Efficiency Xudong Ye, Monsanto Company
11:25	P-3	The EXZACT™ Precision Transformation & Gene Stacking Platform: Design, Development, Deployment and Implications on New Plant Product Discovery and Development Steven R. Webb, Dow AgroSciences
11:50	P-4	Nanocarrier-cargo Technology for Engineering Nucleus and Organelle Genomes Francois Eudes, Agriculture and Agri-food Canada
12:15		Discussion

In Vitro Culture and Micropropagation

Conveners: Ian S. Curtis, Chromatin Inc., and Sylvia A. Mitchell, University of the West Indies

10:30 am – 12:30 pm

Plant Symposium

Ballroom D

The ability to culture plant cells and tissues to the regeneration of fertile plants has great importance for several technologies. Mass micropropagation in vitro of elite plants ,through the in-depth knowledge of culture conditions, nature of the plant material and the associated biochemical reactions, enables the rapid propagation of genetically superior genotypes and disease-tested planting material of economically important plants. The generation of haploid plants, in vitro fertilization, embryo rescue and somatic hybridization help breeders to produce hybrid plants. Advances in recombinant DNA technology, has enabled specific gene traits to be transferred into plants which can accelerate the generation of novel germplasms for breeding programs. The remarkable ability of a single cell to multiply and differentiate into a whole plant has been achieved for many plants. However, there are important dicotyledons and, most notably, monocotyledons that remain recalcitrant to regeneration in culture. This symposium will focus on the application of in vitro approaches to generating valuable plant material for a wealth of commercial industries.

10:30		Introduction (I. S. Curtis and S. A. Mitchell)
10:35	P-5	Innovative Approaches to Optimize the Growth of Protoplasts, Cells and Tissues Mike Davey, University of Nottingham
11:10	P-6	Recalcitrant Radish (Raphanus sativus): Bottlenecks and Successes in Culture Ian S. Curtis, Chromatin Inc
11:45	P-7	Phase Change: a Source of Recalcitrance for the Initiation of Yellow Yam (Dioscorea cayenensis) and Yampie (D. trifida), Two Non-woody Monocotyledenous Tropical Vines Sylvia A. Mitchell, University of the West Indies
12:20		Discussion

The Human Microbiome: What is Known and What Is Next

Conveners: Mae Ciancio, Midwestern University, and Tanja Petnicki-Ocwieja, Tufts University

10:30 am – 12:30 pm

Animal Symposium

Ballroom C

Healthy microbial colonization of mucosal surfaces is achieved through a carefully orchestrated interplay between the host and the microbiota. Changes in this delicate balance can lead to changes in nutrient absorption and utilization, as well as serious chronic inflammatory disorders and autoimmunity. Dysregulation of host defense responses or dysbiosis of microbial communities can result in a breakdown in homeostasis, leading to unwanted and unnecessary inflammatory responses, which can initiate or exacerbate inflammatory disease. With the recent advances in our understanding of the complex composition of the Human Microbiome, this session will focus on the mechanisms required to balance immunological, environmental and microbial factors in healthy mucosa as well as state-of-the-art techniques used to identify changes in microbial communities.

10:30		Introduction (M. Ciancio and T. Petnicki-Ocwieja)
10:35	A-1	The ‘Meta’ Era of Microbiology: Cheaper Sequencing and Bigger Data Dionysios A. Antonopoulous, Argonne National Laboratory
11:10	A-2	Dietary Manipulation of the Gut Microbiota and Its Implications for Tailored Therapies Suzanne Devkota, Harvard Medical School
11:45	A-3	Diversity, Networking and Metabolic Potential of the Digestive Tract of the Healthy Individuals Jacques Izard, The Forsyth Instittute
12:20		Discussion

Forage and Turf Improvement

Convener: Hong Luo, Clemson University

1:30 pm – 3:00 pm

Plant Symposium

Ballroom D

Forage and turf are important components of modern agriculture and ecological systems, contributing significantly to agricultural production, agricultural economy and environmental protection. Trait improvement of forage and turfgrass is important to the forage and turf industry and the environment. Beneficial traits such as herbicide resistance to reduce forage and turfgrass management costs, drought tolerance to reduce water usage, insect and pest resistance to cut pesticide applications, lignin modification to improve digestibility, phyto-remediation of soil contaminants and horticultural qualities among a long list of others, can be improved in forage and turfgrass. With the advance of recombinant DNA and transgenic technologies, GM products with modified characteristics in many row crops have been developed and increasingly accepted world-wide. Similar strategies have also been successfully adopted in forage and turf species for trait modification and demonstrated to be of great value. This symposium will focus on recent progress in exploring biotechnology approaches for use in forage and turf genetic improvement, discussing its great potentials, obstacles, controversies and prospects as well as other important aspects of perennial crop genetic engineering.

1:30		Introduction (H. Luo)
1:35	P-8	Grass Is Greener on the Other Side – Biotechnology for Turfgrass Genetic Improvement Hong Luo, Clemson University
1:55	P-9	Development of Sustainable Turfgrasses Lisa Lee, The Scotts Miracle-Gro Company
2:15	P-10	Improved Agrobacterium-mediated Perennial Ryegrass Transformation Using Novel Approaches Rongda Qu, North Carolina State University
2:35	P-11	Development and Commercialization of Genetically Engineered Alfalfa Products with Enhanced Performance Stephen Temple, Forage Genetics International
2:55		Discussion

New Strategies for Specialty Crops

Conveners: Guo-qing Song, Michigan State University, and Yinghui Dan, IALR/Virginia Tech

1:30 pm – 3:00 pm

Plant Symposium

Ballroom B

Specialty crops are a group of mostly cash-plants including fruits, tree nuts, vegetables, culinary herbs and spices, and medicinal plants. These economically important crops are highly valuable for human nutrition, health, and food security. Due to their importance, specialty crops have been drawing increasing attention from researchers in past few years. For instance, the USDA initiated the Specialty Crop Research Initiative (SCRI) program in 2008, in which one priority is “research in plant breeding, genetics, and genomics to improve crop characteristics”. To date, genomic and biotechnological tools have been developed for many specialty crops. Moving forward with these advancements, this session will discuss the new strategies for specialty crops including Fast-Track breeding, RNAi, intragenic transformation, haploid technology, and integrative biology approaches.

1:30		Introduction (G. Song and Y. Dan)
1:35	P-12	New Strategies for Strawberry Richard E. Veilleux, Virginia Polytechnic Institute and State University
1:55	P-13	Towards Cisgenics to Improve Citrus Health and Nutritional Quality Through Metabolic Engineering Leandro Peña, Instituto Valenciiano de Investigationes Agrarias
2:15	P-14	New Breeding Technologies through Genetic Enginieering in Apple (Malus x domestica) Magda-Viola Hanke, Julius Kühn-Institute
2:35	P-15	A New Strategy to Improve Tomato—Development and Characterization of New Tomato Germplasm with Enhanced Phytonutrients, Using Native Plant DNA Yinghui Dan, Institute for Advanced Learning & Research Virginia Polytechnic Institute And State University
2:55		Discussion

Pathogen-host Interactions and Endocrine Disruptors in Human and Fish

Conveners: Tanja Petnicki-Ocwieja, Tufts University, and Jacques Izard, Forsyth Institute

1:30 pm – 3:00 pm

Animal Symposium

Ballroom C

The interplay between host and pathogen is a complex co-evolutionary battle of surveillance and evasion. The pathogen continues to evolve methods that suppress the host’s mechanisms of detection, while the host develops more intricate methods of defense, which lead to inflammation and disease. However, in many cases the eukaryotic cell and the microbe find ways to co-exist, resulting in healthy colonization of mucosal surfaces. This session will be focused on several important aspects of host-microbial interactions: the recent advances made in our understanding of immune evasion by virulence factors delivered into host cells; the identification of novel mechanisms of pathogen detection; and the role of the immune response in maintaining homeostasis between the eukaryotic cell and commensals.

1:30		Introduction (T. Petnicki-Ocwieja and J. Izard)
1:35	A-4	Mechanisms of Cellular Trafficking That Tailor Toll-like Receptor 2 (TLR2) Responses Tanja Petnicki-Ocwieja, Tufts University School of Medicine
2:00	A-5	Solving Redundancy in a Bacterial Pathogen Using iMAD, a Novel Strategy for Dissecting Complex Interactions Tamara O’Connor, Tufts University School of Medicine
2:25	A-6	Salmonella Typhimurium–host Interactions: A Twist in the Tale of Pathogenesis Beth McCormick, University of Massachusetts Medical School
2:50		Discussion

2013 In Vitro Biology Meeting Opening Ceremony

Program Chair:
Program Co-Chair:

J. Denry Sato, Manzanar Project Foundation
T. Michael Spencer, Monsanto Company

3:15 pm – 5:30 pm

Opening Ceremony

Ballroom B

Conveners: J. Denry Sato, Manzanar Project Foundation, and
David D. Songstad, Cibus LLC

3:15

Welcome and Opening Remarks:
David D. Songstad, President, Society for In Vitro Biology

3:25

ROBERT H. LAWRENCE, JR. KEYNOTE SYMPOSIUM

Introduction (D. D. Songstad)

KS-1

Protein Networks from an Atlas of Proteotypes
Steven P. Briggs, Distinguished Professor, Cell & Developmental Biology, University of California San Diego