Daily Program
Abstracts & Posters
Keynote Speaker Keynote Symposium Plant Contributed Papers
Saturday, June 15 Plenary Symposia Animal Posters
Sunday, June 16 Animal Symposia & Workshops Education Posters
Monday, June 17 Joint Workshop Education Workshop
Tuesday, June 18 Plant Symposia & Workshops Plant Posters
Wednesday, June 19 Animal Contributed Papers Addendum Booklet

Monday, June 17

Daily Program-at-a-Glance

Time Event Room
7:00 am – 6:00 pm Registration Ballroom Foyer
10:00 am – 3:30 pm Exhibits and Posters Ballroom A
Morning 7:00 am – 12:30 pm  
7:00 am – 8:00 pm Publications Committee Meeting Room 554B
8:00 am – 10:00 am Plant and Animal Marine Biology Research Ballroom B
10:00 am – 10:30 am Coffee Break Ballroom A
10:00 am – 10:30 am Education Committee Meeting Room 554A
10:30 am – 11:30 pm Plant Biotechnology Post Doctoral
Oral Presentation Competition
Ballroom B
10:30 am – 12:30 pm Plant Biotechnology Student
Oral Presentation Competition
Ballroom D
10:30 am – 12:30 pm Predictive Toxicology In Vitro: Milestones in Development, Successful Applications and Focus for the Future Ballroom C
11:30 am – 12:30 pm Plant Biotechnology Contributed Paper Session I Ballroom B
Afternoon 12:30 pm – 6:00 pm
12:30 pm – 1:30 pm Membership Committee Meeting Room 554A
12:30 pm – 1:30 pm Refreshment Break Ballroom A
12:30 pm – 2:00 pm Student Networking Luncheon Room 556A/B
1:30 pm – 2:30 pm

Interactive Poster Sessions

Ballroom A
1:30 pm – 2:30 pm In Vitro Animal Cell Sciences Student and Post Doctoral Oral Presentation Competition Ballroom C
2:30 pm -3:30 pm Poster Session
Even Poster Authors will be present
Ballroom A
3:30 pm – 5:00 pm Haploids and Haploidization Ballroom D
3:30 pm – 5:00 pm Non-competitive Student
Oral Presentations
Room 556A/B
3:30 pm – 5:00 pm Rice Biotechnology Ballroom B
3:30 pm – 5:00 pm Three Dimensional Tissues as Powerful Tools for Cancer Research Ballroom C
5:00 pm – 6:00 pm Educational Kits Demonstration Room 556A/B
5:00 pm – 6:00 pm In Vitro Animal Cell Sciences Contributed Papers Ballroom C
5:00 pm – 6:00 pm Plant Biotechnology Contributed Papers II Ballroom B
5:00 pm – 6:00 pm Powerpoint Alternatives and Enhancements for Presentations Ballroom D
Evening 6:00 pm – 10:00 pm
6:00 pm – 7:30 pm In Vitro Animal Cell Sciences Section Business Meeting Ballroom C
6:00 pm – 7:30 pm Plant Biotechnology Section Business Meeting Ballroom B
7:30 pm – 10:00 pm SIVB Sections Social Rotunda

Monday, June 17

Plant and Animal Marine Biology Research

Conveners: Barbara B. Doonan and Robert H. Lawrence

8:00 am – 10:00 am Plenary Symposium Ballroom B

The marine environment has proved to be a wonderful resource in a myriad of ways. Best known perhaps is provision of nutrition via the tremendous variety of edible organisms. Edible seaweeds include Gracilaria, Undaria and Saccharina, and together with the most valued of the maricultured seaweeds, the red alga Porphyra, have a collective value exceeding $U.S. 6.0 billion. In addition to food, seaweeds are used as industrial sources of carrageenans (Kappaphycus), alginates (Saccharina), agars (Gracilaria) and are important in the textile, paper, biotechnological and biomedical industries. These seaweeds also provide unique opportunities in biotechnology, especially in genomics. Microalgae are the basis of the marine ecosystem and ultimate source of fossil fuel because of their photosynthetic carbon fixation. Some (symbiotic dinoflagellates) are indispensible to coral reefs. Many (mainly dinoflagellates) repond to coastal eutrophification by forming harmful algal blooms and producing toxins. Dinoflagellates are also excellent models for understanding bioluminescence and biological clocks. Genomic studies on dinoflagellates and other microalgae are providing valuable insights into how these microorganisms play such important roles in the biosphere and how humans can better exploit their potentials. Animals sourced from the seas around us provide wonderful models for research into specific organs and systems. For example, lampreys have proved to be an excellent model system in studies of neurodegenerative diseases such as Parkinson’s Disease (PD). Lampreys possess a subset of giant neurons that can be identified across all animals. These giant neurons are experimentally tractable and amenable to molecular manipulations. In today’s session, focus will be on (1) work related to the use of seaweeds as nutrient removal systems in integrated multi-trophic aquaculture (IMTA) and nutrient bioextraction systems and (2) studies utilizing a Lamprey model system to aid in elucidation of the underlying molecular mechanisms of aberrant synaptic vesicle trafficking as seen in neurodegenerative diseases such as Parkinson’s Disease (PD).

8:00   Introduction (B. B. Doonan and R. H. Lawrence)
8:05 PS-3 Exploring Integrated Multi-tropic Aquaculture (IMTA) Linkages through Nutrient Bioextraction: The Use of Ecological Methods to Integrate the Cultivation of Seaweeds to Remediate Nutrified Coastal Waters
Charles Yarish, The University of Connecticut
8:40 PS-4 Dinoflagellates: an Unusual Eukaryotic Cell Type
Senjie Lin, University of Connecticut
9:15 PS-5 Identifying the Mechanisms by which Excess α-Synuclein Causes Synaptic Defects in Parkinson’s Disease
Susan Banks, The Eugene Bell Center for Regenerative Biology and Tissue Engineering, Marine Biological Laboratory
9:50   Discussion
10:00 am – 10:30 pm Coffee Break Ballroom A

Plant Biotechnology Post Doctoral Oral Presentation Competition

Moderator: Hong Luo, Clemson University

Panel of Plant Biotechnology Experts Evaluating the Contestants:

Prakash P. Kumar, National University of Singapore, Nancy A. Reichert, Mississippi State University, and Lisa Lee, The Scotts Miracle-Gro Company

10:30 am – 11:30 pm Plant Contributed Paper Competition Ballroom B

To support the Society’s vision to encourage education and scientific informational exchange and recognize outstanding post docs, the Plant Biotechnology Section is pleased to announce the 2013 Post Doctoral Oral Presentation Competition. Post Doctoral candidates wishing to enter submitted a copy of their title and abstract to Dr. Hong Luo. Only those abstracts formally submitted to Dr. Luo were considered. Postdoctoral competition finalists were selected based on the quality of the abstracts. A panel of judges will evaluate the presentations at the meeting. Criteria are experimental design, data analysis, proper interpretation of the results, originality of the study, technical difficulty, appearance and ability of the post doc to explain it. Winners will be presented with a certificate and a cash award at the meeting.

10:30 P-1000 Dissection of the Gmubi Promoter Intron Reveals Possible Regulatory Elements that Affect Promoter Activity and Transgene Expression
Trudi Grant, The Ohio State University-OARDC, C. de la Torre, and J. Finer
10:50 P-1001 GmCPI1, a Soybean Cysteine Protease Inhibitor Is Involved in Plant Development and Response to Biotic Stress
Zhigang Li, Clemson University, April Warner, Qian Hu, Ning Yuan, Cong Li, Benjamin Matthews, Halina Knap, and Hong Luo
11:10 P-1002 Isolated Microspore Culture in Cereals by Mediating Stresses and Nursing
Rakesh Kumar Sinha, Agriculture and Agri-Food Canada, and François Eudes

Plant Biotechnology Student Oral Presentation Competition

Moderator: Rongda Qu, North Carolina State University

Panel of Plant Biotechnology Experts Evaluating the Contestants:

Sylvia A. Mitchell, University of the West Indies, Jyoti R. Rout, Dow AgroSciences, LLC, and Mariya Nikolova Somleva, Metabolix, Inc.

10:30 am – 12:30 pm Plant Contributed Paper Competition Ballroom D

To support the Society’s vision to encourage education and scientific informational exchange and recognize outstanding students, the Plant Biotechnology Section is pleased to announce the 2013 Student Oral Presentation Competition. Student candidates wishing to enter submitted a copy of their title and abstract to Dr. Rongda Qu. Only those abstracts formally submitted to Dr. Qu were considered. Student competition finalists were selected based on the quality of the abstracts. A panel of judges will evaluate the presentations at the meeting. Criteria are experimental design, data analysis, proper interpretation of the results, originality of the study, technical difficulty, appearance and ability of the student to explain it. Winners will be presented with a certificate and a cash award at the meeting.

10:30 P-1003 Improving the Process of Somatic Embryogenesis Using Transient Expression of Transcription Factors and the Implementation of a Novel Bioreactor Design
Sergio Florez, Pennsylvania State University, M. Curtis, S. Maximova, M. Guiltinan and W. Curtis
10:50 P-1004 Insights into the Role of α-Tocopherol in Plants: Multiple Abiotic Stress Tolerance Via ROS Scavenging
Deepak Kumar, Jawaharlal Nehru University, Mohd. Aslam Yusuf, Preeti Singh, Meryam Sardar, and Neera Bhalla Sarin
11:10 P-1005 Increased Mesos Components (CaCl2, MgSO4 and KH2PO4) Improve Growth and Quality of Micropropagated Red Raspberries
Sukalya Poothong, Oregon State University, and Barbara M. Reed
11:30 P-1006 Introgression of a Novel Aldose Reductase from Xerophyta viscose (ALDRXV4) Confers Multiple Stress Tolerance to Evolutionary Diverse Organism
Preeti Singh, Jawaharlal Nehru University, Deepak Kumar, Mohd. Aslam Yusuf, and Neera Bhalla Sarin
11:50 P-1007 Author unable to attend
12:10 P-1008 Engineering Hydroxyproline-O-Glycosylated Peptide Motifs in Hairy Roots for an Enhanced Bioproduction Platform
Ningning Zhang, Arkansas State University, Fabricio Medina-Bolivar, Brett Savary, and Jianfeng Xu

Predictive Toxicology In Vitro: Milestones in Development, Successful Applications and Focus for the Future

Conveners: John W. Harbell and Elizabeth J. Roemer, SUNY – Stony Brook

10:30 am – 12:30 pm Animal Symposium Ballroom C

In vitro toxicity methods were first developed to examine the mechanisms underlying actions of chemicals on organs or tissues observed in vivo. Increasingly, in vitro methods are being applied prospectively in many fields, including cancer biology, drug discovery, and toxicology. These studies are intended to predict the potential action of the test material on the target tissue. Over the past three decades, these efforts in toxicology have moved from qualitative to quantitative prediction of the in vivo effect. Quantitative prediction is essential if the in vitro assay is to replace the in vivo test in the regulatory arena where chemicals and products are characterized according to specific degrees of injury. The impetus to this development has come from several sources: the need to assess toxicity/safety under defined and physiologically relevant exposure conditions (i.e., topical toxicity); an appreciation of the very large number of chemicals that must be characterized (for example in the REACH program); concerns for animal welfare under the 3Rs; and an understanding that traditional animal models (generally rodents) have not provided the predictive capacity required for effective drug development or other applications (Andersen, M.E. and D. Krewski, The vision of toxicity testing in the 21st century: moving from discussion to action. Toxicological Sciences, 2010. 117(1): p. 17-24.). This symposium will be divided into three presentations. The first presentation will discuss lessons learned over the past decade(s) in assay design, development of prediction models, validation, and the beginnings of generalized regulatory acceptance under the OECD test guideline system. The second presentation will focus on current approaches to addressing prediction of topical (eye, skin etc.) and systemic (particularly acute) toxicity in the regulatory framework. The third presentation shares current work on the use of high throughput cell-based screening to identify patterns of toxicity based on disruption of normal metabolic and other activity essential for normal cell function (Toxicology in the 21st Century).

10:30   Introduction (J. Harbell and E. Roemer)
10:35 A-7 Laying the Groundwork for the Development of Validated In Vitro Test Methods for Regulatory Toxicology
John W. Harbell
11:10 A-8 Current In Vitro Test Methods for Classification and Labeling of Chemicals and Products
Hans Raabe, Institute for In Vitro Sciences
11:45 A-9 Tox21 Overview and Update
Warren Casey, NIH/NIEHS
12:20   Discussion

Plant Biotechnology Contributed Paper Session I

Moderator: Zhanyuan Zhang, University of Missouri

11:30 am – 12:30 pm Plant Contributed Paper Session Ballroom B
11:30 P-1009 Toward Chromosome Length Contigs: Single-molecule, Electronic, Solid-State Sequencing of Long DNA Fragments
John Oliver, NABsys Inc., Debra Dederich, Peter Goldstein, William Heaton, Dona Hevroni, Maryam Jouzi, Hsu-Yi Lee, Mark Nadel, and John Thompson
11:42 P-1010 Micropropagation Integrated with Fingerling Culture Method for Cost Effective Clonal Plant Production
C. Sudhersan, Kuwait Institute for Scientific Research, and J. Ashkanani
11:54 P-1011 An Improved Microinjection Method for Agrobacterium tumefaciens Mediated Genetic Transformation in Tomato
N. Jayabalan, Bharathidasan University, S. Vinoth and P. Gurusaravanan
12:06 P-1012 Enhanced Production of Edible Food Colorants in Growtek Bioreactor by a Callus Line of Amaranthus tricolor L.
Satyahari Dey, Indian Institute of Technology, and Mousumi Biswas
12:18 P-1013 RNAi-mediated Resistance to Cucumber Mosaic Virus (CMV) in Transgenic Tomato
Valentine Otang Ntui, Chiba University, Kong Kynet, Ikuo Nakamura, and Masahiro Mii

Student Networking Luncheon

Conveners: Sydney Shaw, Penn State University, and Jeff Kwak, Midwestern University

12:30 pm – 2:00 pm Education Workshop Room 556A/B

Networking is an essential part of a student’s career that is particularly critical during the transition from student to professional. The Society for In Vitro Biology’s meeting brings together a diverse group of international professionals who are interested in forming relationships with students who will soon be their colleagues. This forum is a great opportunity to talk to both students and renowned SIVB members in order to practice networking skills and continue to expand your own professional network. Join us for an informal discussion with leaders in industry and academia who will share their professional experiences, talk about trends in their field, and pass along some advice they have collected throughout their careers.

12:30 Introduction (S. Shaw and J. Kwak)
12:40 Panel Discussion
  Panelists:
  Mae Ciancio, Midwestern University
  Wayne Curtis, Penn State University
  Michael Fay, Midwestern University
  Ted Klein, DuPont Pioneer
  Kolla Kristjansdottir, Midwestern University
  Todd J. Jones, Pioneer Hi-Bred International
  T. Michael Spencer, Monsanto Company
  Pam Weathers, Worcester Polytechnic Institute

Cellular Models for Preservation, Toxicity, and Cancer Biology

Moderator: Addy Alt-Holland, Tufts University, and Jeff Kwak, Midwestern University

1:30 pm – 2:30 pm Animal Interactive Poster Session Ballroom A
A-2000 Post-mortem Recovery of Fibroblast-like Cells in Sheep after 10 Days of Animal Death
M. Singh, Fort Valley State University, and X. Ma
A-2001 Author is unable to attend
A-2002 Suitability of Invertebrate and Vertebrate Cells in a Portable Impedance-based Toxicity Sensor: Temperature Mediated Impacts on Long-term Survival
T. M. Curtis, State University of New York at Cortland, A. Howard, B. D. Gerlach, L. M. Brennan, M. W. Widder, W. H. van der Schalie, N. T. K. Vo, and N. C. Bols
A-2003 Prevention of Chemically-induced Inhibition of Gap-junctional Intercellular Communication by Phytochemicals – In Vitro Biomarker of Chemopreventive Effects
Brad L. Upham, Michigan State University, Z. Lencesova, J. E. Trosko, and P. Babica
A-2004 Characterization A-549 Lung Adenocarcinoma Metastasis in 2D and 3D Environments
Venkata Arun Timmaraju, University of New Haven, and Michael J. Rossi
A-2005 Regulation of RNA Polymerase III Transcription by EGCG in Lungs
Nicole Nole and Jana Suchtova, St. John’s University, and Laura Schramm

In Vitro Animal Cell Sciences Student and Post Doctoral Oral Presentation Competition

Panel of In Vitro Animal Cell Sciences Experts Evaluating the Contestants:

Adam Sowalsky, Harvard Medical School, James Baleja, Tufts University School of Medicine, Guy Smagghe, University fo Ghent, Addy Alt-Holland, Tufts University, Jeffrey Kwak, Midwestern University, and Shruti Pore, Tufts University School of Dental Medicine

3:30 pm – 5:00 pm Animal Contributed Paper Competition Ballroom C

To support the Society’s vision to encourage education and scientific informational exchange and recognize outstanding students and post docs, the In Vitro Animal Cell Sciences section is pleased to announce the 2013 In Vitro Animal Cell Science Research Oral Presentation Competitions for both Students and Post Docs. Students and Post Docs wishing to enter submitted a copy of their title and abstract (as submitted to SIVB) and abstract tracking number to Dr. Addy Alt-Holland. Only those abstracts formally submitted to both SIVB and Dr. Alt-Holland were considered. Finalists were selected based on the quality of the abstracts. A panel of judges will evaluate the presentations at the meeting. Criteria are experimental design, data analysis, proper interpretation of the results, originality of the study, technical difficulty, appearance of the presentation and ability of the finalist to respond to questions. Winners will be presented with a certificate and a cash award at the meeting.

1:30 A-1000 Development of Cell Lines from the Walleye Caudal Fin and Their Ability to Survive and Support Viral Hemorrhagic Septicemia Virus (VHSV) Group IVb Replication at Low Temperatures
Aaron W. Bender, University of Waterloo, N. T. K. Vo, P. A. L. Rivers, J. S. Lumsden, B. Dixon, and N. C. Bols
1:50 A-1001 The Susceptibility of Walleye Cell Lines from the Spleen Stroma and Caudal Fin to Viral Hemorrhagic Septicemia Virus (VHSV)
Nguyen T. K. Vo, University of Waterloo, Hina A. Bandukwala, Aaron W. Bender, John S. Lumsden, Lucy E. J. Lee, Brian Dixon, and Niels C. Bols
2:10 A-1002 Effects of Differential Trk Expression on Neuroblastoma Cells Cultured in the Microgravity Rotary Bioreactor
Robert A. Redden, The Children’s Hospital of Philadelphia, Jane E. Minturn, Garrett M. Brodeur, and Edward J. Doolin

In Vitro Culture/Micropropagation

Moderator: Matthew Curtis, The Pennsylvania State University

1:30 pm – 2:30 pm Plant Interactive Poster Session Ballroom A
P-2000 Biosynthesis Enhancement of Stilbenoids in Hairy Root Cultures of Peanut
L. Ogutu, Arkansas State University, L. Nopo-Olazabal, and F. Medina-Bolivar
P=2001 Novel Temporary Immersion Bioreactor Allows the Manipulation Headspace Composition to Improve Plant Tissue Propagation
M. Curtis, The Pennsylvania State University, S. Florez, and W. Curtis
P-2002 Shoot Organogenesis from In Vitro Derived Leaf Tissues of Piper longum
D. Rani, Dayalbagh Educational Institute (Deemed University), and P. K. Dantu
P-2004 Towards Automation of Micropropagation Using a Mist Bioreactor
L. Fei, Worcester Polytechnic Institute, and P. J. Weathers

Plant Biotechnology

Moderator: Liu Y. Shen, Dow AgroSciences

1:30 pm – 2:30 pm Plant Interactive Poster Session Ballroom A
P-2006 Identification and Characterization of the Genes Involved in the Fruit Color Development of European Plums (Prunus domestica L.)
S. Dinesh Kumar, University of Guelph, S. Sherif, K. S. Tiwari, G. Paliyath, and S. Jayasankar
P-2007 Physiological and Molecular Studies of Salt Stress on Camelina sativa
D. Morales and D. Miller, Shippensburg University of Pennsylvania, A. Jones, E. Kovak, E. Lowry, F. Ansary, R. Anand, N. Karelia, A. Shuler, S. Rudrabhatla, and S. Potlakayla
P-2008 Ectopic Expression of an Osmotin in Indian Soybean Confers Resistance Against Drought Stress
Kondeti Subramanyam, Bharathidasan University, Andy Ganapathi, and Markandan Manickavasagam
P-2009 In-planta Transformation of Jatropha curcas (L.) and Ex Vitro Multiplication of Transgenics Using Grafting
Balusamy Jaganath, Bharathidasan University, Andy Ganapathi, and Markandan Manickavasagam
P-2010 Genetic Engineering of Fruit Rot Disease Resistance in Capsicum annuum. L Using Defensin gene (TvD1) Through Stable Plastid Transformation
A. V. Rao, Kakatiya University, N. Murali Krishna, E. Raghu, and A. Sadanandam
P-2011 Tissue Culture, Transformation, and Genetic Diversity Studies of Jatropha curcas, a Second Generation Biofuel Crop
Behnam Tabatabai, Penn State Harrisburg, Mohsen Zaidi, Matthew Reitzel, Shobha Potlakayala, Lauren Kong, Camille Gonzalez-Morales, Alison Shuler, and Sairam Rudrabhatla
P-2012 Development of Brassica juncea Suspension Culture and Its Transformation Using Particle Bombardment
Liu Y. Shen, Dow AgroSciences

Haploids and Haploidization

Convener: Joseph F. Petolino, Dow AgroSciences

3:30 pm – 5:00 pm Plant Symposium Ballroom D

Haploids are invaluable materials for basic genetic studies as well as for applied breeding and biotechnology. The instantaneous line fixation that accompanies the doubling of the chromosome comlement of haploids enhances the efficiency of inbred line development and selection. The totipotent microspore represents an attractive target for transgene delivery. Functional genomics, of polyploid species in particular, become more powerful with the availability of haploid production technology. This session will highlight some in vitro methods used to generate and employ haploids for crop improvement.

3:30   Introduction (J. Petolino)
3:35 P-16 Haploids in Plant Breeding and Genomic Research
Tatiana Boluarte-Medina, Virginia Tech
4:00 P-17 Isolated Microspore Culture in Cereals by Mediating Stresses and Nursing
Rakesh Sinha, Agriculture and Agri-Food Canada
4:25 P-18 Improving Camelina Doubled Haploidy: Microspore Transformation Using Nanocarriers
Alison Ferrie, Plant Biotechnology Institute
4:50   Discussion

Non-competitive Student Oral Presentations

Conveners: Jessica L. Rupp, Kansas State University, and Kerri Neugebauer, Kansas State University

3:30 pm – 5:00 pm Education Workshop Room 556A/B

Public speaking and presenting research findings are a large part of a scientific career. What better way to become proficient at oral presentations than to practice? If your abstract was not chosen for the competitive session, and you would still like an opportunity to present your research, this is the session for you! This new session will allow speakers to practice their public speaking skills in a relaxed, friendly environment without the pressure of being in a competition. All students, even those that don’t submit an abstract to the oral competition, are welcome to apply to speak at this session. To make this a learning experience for everyone involved, the speaker will receive constructive criticism from other members and students of SIVB by using the same judging rubric from the competitive session. Join us to practice speaking and share your research!

Participants:

A-2005 Regulation of RNA Polymerase III Transcription by EGCG in Lungs
Nicole Nole and Jana Suchtova, St. John’s University, and Laura Schramm
P-2002 Shoot Organogenesis from In Vitro Derived Leaf Tissues of Piper longum
D. Rani, Dayalbagh Educational Institute (Deemed University), and P. K. Dantu
P-2038 Effect of Aluminum on Ex Vitro Rooting of Different Clones of Tectona grandis Lf Propagated In Vitro
R. S. Barbosa, University of São Paulo, and A. N. Gonçalves
P-2055 Comparison of Larvicidal Effect of Rhizomes of Tissue Cultured and Naturally Grown Plants of Kaempferia galanga (Zengiberaceae)
R. M. U. S. Senarath, Virgen Milagrosa University Foundation, Jimmy G. Catanes, and S. Senarath

Rice Biotechnology

Conveners: Prakash P. Kumar, National University of Singapore, and Vibha Srivastava, University of Arkansas

3:30 pm – 5:00 pm Plant Symposium Ballroom B

With about 140 million hectares annually under rice cultivation in Asia, rice is one of the most important staple food crops. Rice production ensures food security for more than half the world population. Rice-related industries have multibillion dollar annual turnover and it accounts for employing more people than any other single industry in Asia. Rice production is facing serious constraints especially due to global environmental changes. The productivity gains from the post-Green Revolution era of 1970s have attained saturation. Such yield stagnation is compounded by the declining arable land, water scarcity and global climate change. According to IRRI and the International Food Policy Research Institute USA, the price of rice will double by 2050 due to the combined effect of adverse climate changes on the crop and high demands for food grains due to population increase. Therefore, investments in rice research are essential to ensure and protect future rice supplies. Besides the R&D efforts of a number of agbiotech companies, initiatives such as the Global Rice Science Partnership (GRiSP) coordinated by the IRRI are attempting to address this. An integrated application of modern technologies including biotechnology for improving the crop is imperative in order to adapt the currently accepted varieties of rice to the changing environments and ensure stable rice supply.

3:30   Introduction (P. P. Kumar and V. Srivastava)
3:35 P-19 Use of Metabolomics to Characterize a Dwarf Mutant Line of Rice
Prakash P. Kumar, National University of Singapore
4:00 P-20 A Field Validated and Versatile QTL for Drought Tolerance in Rice
Ajay Kohli, International Rice Research Institute
4:25 P-21 Biotechnological Approaches to Boost Rice Innate Immunity for Broad-Spectrum and Durable Disease Resistance
Yinong Yang, Penn State University
4:50   Discussion

Three Dimensional Tissues as Powerful Tools for Cancer Research

Conveners: Addy Alt-Holland, Tufts University, Michael Dame, University of Michigan Medical School, and Brad Upham, Michigan State University

3:30 pm – 5:00 pm Animal Symposium Ballroom C

Modeling cancer development in bioengineered tissues, which mimic the features of their in vivo counterparts, enables cancer researchers to narrow the gap between experiments performed in monolayer cell cultures and in vivo. Significantly, three-dimensional tissue models and organ cultures allow for marinating dynamic and biologically meaningful interactions between different cell types, in a complex and supportive microenvironment. This symposium will focus on bioengineered, physiologically relevant, human skin tissues that consist of dermal and epidermal compartments, as well as on organ cultures from human skin biopsies. These tissues are used in the quest to reveal molecular mechanisms and signaling pathways that regulate the fundamental cellular processes of tumor cell motility and invasion. Novel therapeutic approaches designed to target these critical processes will potentially abrogate the progression of squamous cell carcinoma in the human skin.

3:30   Introduction (A. Alt-Holland, M. Dame and B. L. Upham)
3:35 A-10 RalA: A Double Edged Sword in Skin Squamous Cell Carcinoma
Adam G. Sowalsky, Harvard Medical School
4:00 A-11 The Role of Dab2 in Directing the Pathogenesis of Human Skin Squamous Cell Carcinoma
James Baleja, Tufts University School of Medicine
4:25 A-12 Organ-cultured Human Skin to Study Squamous Epithelial Cell Invasion
James Varani, The University of Michigan
4:50   Discussion

Educational Kits Demonstration

Convener: Sylvia A. Mitchell, University of the West Indies

5:00 pm – 6:00 pm Education Workshop Room 556A/B

This educational workshop will describe how to implement multiplex measurement of cell viability, necrosis, and apoptosis from the same sample of cells using a standard multimode plate reader. A detailed description of preparing cells for the assay, performing the sequential homogeneous add-mix-measure multiplex protocol, and analysis of example data will be presented. The advantages of using multiplexing as an internal control for cell-based assays will be described.

5:00   Introduction (S.A. Mitchell)
5:05 E-1 Education Kit for Determining Cell Viability, Death and Apoptosis from the Same Sample
Terry Riss, Promega
5:50   Discussion

In Vitro Animal Cell Sciences Contributed Papers

Moderators: Brad L. Upham, Michigan State University, and Shruti Pore, Tufts University School of Dental Medicine

5:00 pm – 6:00 pm Animal Contributed Paper Session Ballroom C
5:00 A-1003 Comparison of Human Colonic Crypts and Abnormal Crypts from Adenoma in Culture: A Multi-stage Model to Assess Colon Chemoprevention
Y. Jiang, University of Michigan Medical School, M. K. Dame, D. K. Turgeon, H. Appelman, M. N. Aslam, K. Copley, D. Attili, D. Brenner, and J. Varani
5:15 A-1004 Voluntary Exercise Prevents Obesity and Significantly Alters Major Gut Bacteria Phyla
Jeff Kwak, Midwestern University, Christian C. Evans, Samantha Laskowski, Joseph Dougherty, Kathy J. LePard, and Mae J. Ciancio
5:30 A-1005 The p53 Inhibitor, Pifithrin-µ , Induces Apoptosis in Rainbow Trout Gill Cell Line, RTgill-W1
F. Zeng, University of Waterloo, J. P. Sherry, B. Dixon, B. P. Duncker, N. C. Bols
5:45 A-1006 Using Fish Cell Lines to Study How Engineered Nanomaterials Influence the Survival of Viruses from Lower Aquatic Vertebrates
Y. J. Huang, University of Waterloo, P. H. Pham, A. Hu, K. Oakes, S. X. Tang, and N. C. Bols

Plant Biotechnology Contributed Papers II

Moderator: Zhanyuan Zhang, University of Missouri

5:00 pm – 6:00 pm Plant Contributed Paper Session Ballroom B
5:00 P-1014 Transcriptional Activity and Practical Use of Ubiquitin and PR-1 Gene Promoters from Grapevine (Vitis spp.) in Transgenic Plant Recovery
D. J. Gray, University of Florida/IFAS, Z. T. Li, K.-H. Kim, J. R. Jasinski, D. D. Silva, and M. R. Creech
5:15 P-1015 Hydroxyproline-O-Glycosylated Biopolymer Carriers for Competitive Plant Cell and Tissue Bioproduction Platforms
Jianfeng Xu, Arkansas State University, Maureen Dolan, Ningning Zhang, and Marcia Kieliszewski
5:30 P-1016 Synthetic TAL Effectors for Targeted Gene Activation in Plants
C. Neal Stewart, Jr., The University of Tennessee, Wusheng Liu, Mary R. Rudis, Yanhui Peng, Mitra Mazarei, Reginald J. Millwood, Jian-Ping Yang, and Jonathan D. Chesnut
5:45 P-1017 Somatic Embryogenesis from Immature Embryos for the Transformation and Regeneration of Sorghum Lines (Sorghum bicolor L.)
Shireen K. Assem, Agricultural Genetic Engineering Research Institute, S. H. El-Mansy, M. M. Zamzam, and E. H. A. Hussein

Powerpoint and Enhancements for Presentations

Facilitator: James J. Sadler, University of Florida

5:00 pm – 6:00 pm Education Workshop Ballroom D

Presenters and teachers traditionally use presentation software to deliver lectures with supporting graphics. Over the past decade, PowerPoint has become the dominant software for teachers and researchers. However, other options are available that could provide a better vehicle for presenting information. Prezi, a new presentation program, will be introduced as an alternative to PowerPoint. Advantages and challenges, in addition to the potential uses, of Prezi will be discussed among the audience members to generate “etiquette” for using this program. Cloud presentation programs, such as GoogleDocs and SlideRocket, will be discussed including their ability to help multi-institutional development of presentations. Finally, PowerPoint presentation enhancements will be presented for those who want to continue using PowerPoint. In addition to presentations, alternatives and enhancements to posters will be presented and discussed.