Monday, June 17
Daily Program-at-a-Glance
Time | Event | Room |
---|---|---|
7:00 am – 6:00 pm | Registration | Ballroom Foyer |
10:00 am – 3:30 pm | Exhibits and Posters | Ballroom A |
Morning | 7:00 am – 12:30 pm | |
7:00 am – 8:00 pm | Publications Committee Meeting | Room 554B |
8:00 am – 10:00 am | Plant and Animal Marine Biology Research | Ballroom B |
10:00 am – 10:30 am | Coffee Break | Ballroom A |
10:00 am – 10:30 am | Education Committee Meeting | Room 554A |
10:30 am – 11:30 pm | Plant Biotechnology Post Doctoral Oral Presentation Competition |
Ballroom B |
10:30 am – 12:30 pm | Plant Biotechnology Student Oral Presentation Competition |
Ballroom D |
10:30 am – 12:30 pm | Predictive Toxicology In Vitro: Milestones in Development, Successful Applications and Focus for the Future | Ballroom C |
11:30 am – 12:30 pm | Plant Biotechnology Contributed Paper Session I | Ballroom B |
Afternoon | 12:30 pm – 6:00 pm | |
12:30 pm – 1:30 pm | Membership Committee Meeting | Room 554A |
12:30 pm – 1:30 pm | Refreshment Break | Ballroom A |
12:30 pm – 2:00 pm | Student Networking Luncheon | Room 556A/B |
1:30 pm – 2:30 pm |
Interactive Poster Sessions |
Ballroom A |
1:30 pm – 2:30 pm | In Vitro Animal Cell Sciences Student and Post Doctoral Oral Presentation Competition | Ballroom C |
2:30 pm -3:30 pm | Poster Session Even Poster Authors will be present |
Ballroom A |
3:30 pm – 5:00 pm | Haploids and Haploidization | Ballroom D |
3:30 pm – 5:00 pm | Non-competitive Student Oral Presentations |
Room 556A/B |
3:30 pm – 5:00 pm | Rice Biotechnology | Ballroom B |
3:30 pm – 5:00 pm | Three Dimensional Tissues as Powerful Tools for Cancer Research | Ballroom C |
5:00 pm – 6:00 pm | Educational Kits Demonstration | Room 556A/B |
5:00 pm – 6:00 pm | In Vitro Animal Cell Sciences Contributed Papers | Ballroom C |
5:00 pm – 6:00 pm | Plant Biotechnology Contributed Papers II | Ballroom B |
5:00 pm – 6:00 pm | Powerpoint Alternatives and Enhancements for Presentations | Ballroom D |
Evening | 6:00 pm – 10:00 pm | |
6:00 pm – 7:30 pm | In Vitro Animal Cell Sciences Section Business Meeting | Ballroom C |
6:00 pm – 7:30 pm | Plant Biotechnology Section Business Meeting | Ballroom B |
7:30 pm – 10:00 pm | SIVB Sections Social | Rotunda |
Monday, June 17
Plant and Animal Marine Biology Research
Conveners: Barbara B. Doonan and Robert H. Lawrence
8:00 am – 10:00 am | Plenary Symposium | Ballroom B |
The marine environment has proved to be a wonderful resource in a myriad of ways. Best known perhaps is provision of nutrition via the tremendous variety of edible organisms. Edible seaweeds include Gracilaria, Undaria and Saccharina, and together with the most valued of the maricultured seaweeds, the red alga Porphyra, have a collective value exceeding $U.S. 6.0 billion. In addition to food, seaweeds are used as industrial sources of carrageenans (Kappaphycus), alginates (Saccharina), agars (Gracilaria) and are important in the textile, paper, biotechnological and biomedical industries. These seaweeds also provide unique opportunities in biotechnology, especially in genomics. Microalgae are the basis of the marine ecosystem and ultimate source of fossil fuel because of their photosynthetic carbon fixation. Some (symbiotic dinoflagellates) are indispensible to coral reefs. Many (mainly dinoflagellates) repond to coastal eutrophification by forming harmful algal blooms and producing toxins. Dinoflagellates are also excellent models for understanding bioluminescence and biological clocks. Genomic studies on dinoflagellates and other microalgae are providing valuable insights into how these microorganisms play such important roles in the biosphere and how humans can better exploit their potentials. Animals sourced from the seas around us provide wonderful models for research into specific organs and systems. For example, lampreys have proved to be an excellent model system in studies of neurodegenerative diseases such as Parkinson’s Disease (PD). Lampreys possess a subset of giant neurons that can be identified across all animals. These giant neurons are experimentally tractable and amenable to molecular manipulations. In today’s session, focus will be on (1) work related to the use of seaweeds as nutrient removal systems in integrated multi-trophic aquaculture (IMTA) and nutrient bioextraction systems and (2) studies utilizing a Lamprey model system to aid in elucidation of the underlying molecular mechanisms of aberrant synaptic vesicle trafficking as seen in neurodegenerative diseases such as Parkinson’s Disease (PD).
8:00 | Introduction (B. B. Doonan and R. H. Lawrence) | |
8:05 | PS-3 | Exploring Integrated Multi-tropic Aquaculture (IMTA) Linkages through Nutrient Bioextraction: The Use of Ecological Methods to Integrate the Cultivation of Seaweeds to Remediate Nutrified Coastal Waters Charles Yarish, The University of Connecticut |
8:40 | PS-4 | Dinoflagellates: an Unusual Eukaryotic Cell Type Senjie Lin, University of Connecticut |
9:15 | PS-5 | Identifying the Mechanisms by which Excess α-Synuclein Causes Synaptic Defects in Parkinson’s Disease Susan Banks, The Eugene Bell Center for Regenerative Biology and Tissue Engineering, Marine Biological Laboratory |
9:50 | Discussion |
10:00 am – 10:30 pm | Coffee Break | Ballroom A |
Plant Biotechnology Post Doctoral Oral Presentation Competition
Moderator: Hong Luo, Clemson University
Panel of Plant Biotechnology Experts Evaluating the Contestants:
Prakash P. Kumar, National University of Singapore, Nancy A. Reichert, Mississippi State University, and Lisa Lee, The Scotts Miracle-Gro Company
10:30 am – 11:30 pm | Plant Contributed Paper Competition | Ballroom B |
To support the Society’s vision to encourage education and scientific informational exchange and recognize outstanding post docs, the Plant Biotechnology Section is pleased to announce the 2013 Post Doctoral Oral Presentation Competition. Post Doctoral candidates wishing to enter submitted a copy of their title and abstract to Dr. Hong Luo. Only those abstracts formally submitted to Dr. Luo were considered. Postdoctoral competition finalists were selected based on the quality of the abstracts. A panel of judges will evaluate the presentations at the meeting. Criteria are experimental design, data analysis, proper interpretation of the results, originality of the study, technical difficulty, appearance and ability of the post doc to explain it. Winners will be presented with a certificate and a cash award at the meeting.
10:30 | P-1000 | Dissection of the Gmubi Promoter Intron Reveals Possible Regulatory Elements that Affect Promoter Activity and Transgene Expression Trudi Grant, The Ohio State University-OARDC, C. de la Torre, and J. Finer |
10:50 | P-1001 | GmCPI1, a Soybean Cysteine Protease Inhibitor Is Involved in Plant Development and Response to Biotic Stress Zhigang Li, Clemson University, April Warner, Qian Hu, Ning Yuan, Cong Li, Benjamin Matthews, Halina Knap, and Hong Luo |
11:10 | P-1002 | Isolated Microspore Culture in Cereals by Mediating Stresses and Nursing Rakesh Kumar Sinha, Agriculture and Agri-Food Canada, and François Eudes |
Plant Biotechnology Student Oral Presentation Competition
Moderator: Rongda Qu, North Carolina State University
Panel of Plant Biotechnology Experts Evaluating the Contestants:
Sylvia A. Mitchell, University of the West Indies, Jyoti R. Rout, Dow AgroSciences, LLC, and Mariya Nikolova Somleva, Metabolix, Inc.
10:30 am – 12:30 pm | Plant Contributed Paper Competition | Ballroom D |
To support the Society’s vision to encourage education and scientific informational exchange and recognize outstanding students, the Plant Biotechnology Section is pleased to announce the 2013 Student Oral Presentation Competition. Student candidates wishing to enter submitted a copy of their title and abstract to Dr. Rongda Qu. Only those abstracts formally submitted to Dr. Qu were considered. Student competition finalists were selected based on the quality of the abstracts. A panel of judges will evaluate the presentations at the meeting. Criteria are experimental design, data analysis, proper interpretation of the results, originality of the study, technical difficulty, appearance and ability of the student to explain it. Winners will be presented with a certificate and a cash award at the meeting.
10:30 | P-1003 | Improving the Process of Somatic Embryogenesis Using Transient Expression of Transcription Factors and the Implementation of a Novel Bioreactor Design Sergio Florez, Pennsylvania State University, M. Curtis, S. Maximova, M. Guiltinan and W. Curtis |
10:50 | P-1004 | Insights into the Role of α-Tocopherol in Plants: Multiple Abiotic Stress Tolerance Via ROS Scavenging Deepak Kumar, Jawaharlal Nehru University, Mohd. Aslam Yusuf, Preeti Singh, Meryam Sardar, and Neera Bhalla Sarin |
11:10 | P-1005 | Increased Mesos Components (CaCl2, MgSO4 and KH2PO4) Improve Growth and Quality of Micropropagated Red Raspberries Sukalya Poothong, Oregon State University, and Barbara M. Reed |
11:30 | P-1006 | Introgression of a Novel Aldose Reductase from Xerophyta viscose (ALDRXV4) Confers Multiple Stress Tolerance to Evolutionary Diverse Organism Preeti Singh, Jawaharlal Nehru University, Deepak Kumar, Mohd. Aslam Yusuf, and Neera Bhalla Sarin |
11:50 | P-1007 | Author unable to attend |
12:10 | P-1008 | Engineering Hydroxyproline-O-Glycosylated Peptide Motifs in Hairy Roots for an Enhanced Bioproduction Platform Ningning Zhang, Arkansas State University, Fabricio Medina-Bolivar, Brett Savary, and Jianfeng Xu |
Predictive Toxicology In Vitro: Milestones in Development, Successful Applications and Focus for the Future
Conveners: John W. Harbell and Elizabeth J. Roemer, SUNY – Stony Brook
10:30 am – 12:30 pm | Animal Symposium | Ballroom C |
In vitro toxicity methods were first developed to examine the mechanisms underlying actions of chemicals on organs or tissues observed in vivo. Increasingly, in vitro methods are being applied prospectively in many fields, including cancer biology, drug discovery, and toxicology. These studies are intended to predict the potential action of the test material on the target tissue. Over the past three decades, these efforts in toxicology have moved from qualitative to quantitative prediction of the in vivo effect. Quantitative prediction is essential if the in vitro assay is to replace the in vivo test in the regulatory arena where chemicals and products are characterized according to specific degrees of injury. The impetus to this development has come from several sources: the need to assess toxicity/safety under defined and physiologically relevant exposure conditions (i.e., topical toxicity); an appreciation of the very large number of chemicals that must be characterized (for example in the REACH program); concerns for animal welfare under the 3Rs; and an understanding that traditional animal models (generally rodents) have not provided the predictive capacity required for effective drug development or other applications (Andersen, M.E. and D. Krewski, The vision of toxicity testing in the 21st century: moving from discussion to action. Toxicological Sciences, 2010. 117(1): p. 17-24.). This symposium will be divided into three presentations. The first presentation will discuss lessons learned over the past decade(s) in assay design, development of prediction models, validation, and the beginnings of generalized regulatory acceptance under the OECD test guideline system. The second presentation will focus on current approaches to addressing prediction of topical (eye, skin etc.) and systemic (particularly acute) toxicity in the regulatory framework. The third presentation shares current work on the use of high throughput cell-based screening to identify patterns of toxicity based on disruption of normal metabolic and other activity essential for normal cell function (Toxicology in the 21st Century).
10:30 | Introduction (J. Harbell and E. Roemer) | |
10:35 | A-7 | Laying the Groundwork for the Development of Validated In Vitro Test Methods for Regulatory Toxicology John W. Harbell |
11:10 | A-8 | Current In Vitro Test Methods for Classification and Labeling of Chemicals and Products Hans Raabe, Institute for In Vitro Sciences |
11:45 | A-9 | Tox21 Overview and Update Warren Casey, NIH/NIEHS |
12:20 | Discussion |
Plant Biotechnology Contributed Paper Session I
Moderator: Zhanyuan Zhang, University of Missouri
11:30 am – 12:30 pm | Plant Contributed Paper Session | Ballroom B |
11:30 | P-1009 | Toward Chromosome Length Contigs: Single-molecule, Electronic, Solid-State Sequencing of Long DNA Fragments John Oliver, NABsys Inc., Debra Dederich, Peter Goldstein, William Heaton, Dona Hevroni, Maryam Jouzi, Hsu-Yi Lee, Mark Nadel, and John Thompson |
11:42 | P-1010 | Micropropagation Integrated with Fingerling Culture Method for Cost Effective Clonal Plant Production C. Sudhersan, Kuwait Institute for Scientific Research, and J. Ashkanani |
11:54 | P-1011 | An Improved Microinjection Method for Agrobacterium tumefaciens Mediated Genetic Transformation in Tomato N. Jayabalan, Bharathidasan University, S. Vinoth and P. Gurusaravanan |
12:06 | P-1012 | Enhanced Production of Edible Food Colorants in Growtek Bioreactor by a Callus Line of Amaranthus tricolor L. Satyahari Dey, Indian Institute of Technology, and Mousumi Biswas |
12:18 | P-1013 | RNAi-mediated Resistance to Cucumber Mosaic Virus (CMV) in Transgenic Tomato Valentine Otang Ntui, Chiba University, Kong Kynet, Ikuo Nakamura, and Masahiro Mii |
Student Networking Luncheon
Conveners: Sydney Shaw, Penn State University, and Jeff Kwak, Midwestern University
12:30 pm – 2:00 pm | Education Workshop | Room 556A/B |
Networking is an essential part of a student’s career that is particularly critical during the transition from student to professional. The Society for In Vitro Biology’s meeting brings together a diverse group of international professionals who are interested in forming relationships with students who will soon be their colleagues. This forum is a great opportunity to talk to both students and renowned SIVB members in order to practice networking skills and continue to expand your own professional network. Join us for an informal discussion with leaders in industry and academia who will share their professional experiences, talk about trends in their field, and pass along some advice they have collected throughout their careers.
12:30 | Introduction (S. Shaw and J. Kwak) |
12:40 | Panel Discussion |
Panelists: | |
Mae Ciancio, Midwestern University | |
Wayne Curtis, Penn State University | |
Michael Fay, Midwestern University | |
Ted Klein, DuPont Pioneer | |
Kolla Kristjansdottir, Midwestern University | |
Todd J. Jones, Pioneer Hi-Bred International | |
T. Michael Spencer, Monsanto Company | |
Pam Weathers, Worcester Polytechnic Institute |
Cellular Models for Preservation, Toxicity, and Cancer Biology
Moderator: Addy Alt-Holland, Tufts University, and Jeff Kwak, Midwestern University
1:30 pm – 2:30 pm | Animal Interactive Poster Session | Ballroom A |
A-2000 | Post-mortem Recovery of Fibroblast-like Cells in Sheep after 10 Days of Animal Death M. Singh, Fort Valley State University, and X. Ma |
A-2001 | Author is unable to attend |
A-2002 | Suitability of Invertebrate and Vertebrate Cells in a Portable Impedance-based Toxicity Sensor: Temperature Mediated Impacts on Long-term Survival T. M. Curtis, State University of New York at Cortland, A. Howard, B. D. Gerlach, L. M. Brennan, M. W. Widder, W. H. van der Schalie, N. T. K. Vo, and N. C. Bols |
A-2003 | Prevention of Chemically-induced Inhibition of Gap-junctional Intercellular Communication by Phytochemicals – In Vitro Biomarker of Chemopreventive Effects Brad L. Upham, Michigan State University, Z. Lencesova, J. E. Trosko, and P. Babica |
A-2004 | Characterization A-549 Lung Adenocarcinoma Metastasis in 2D and 3D Environments Venkata Arun Timmaraju, University of New Haven, and Michael J. Rossi |
A-2005 | Regulation of RNA Polymerase III Transcription by EGCG in Lungs Nicole Nole and Jana Suchtova, St. John’s University, and Laura Schramm |
In Vitro Animal Cell Sciences Student and Post Doctoral Oral Presentation Competition
Panel of In Vitro Animal Cell Sciences Experts Evaluating the Contestants:
Adam Sowalsky, Harvard Medical School, James Baleja, Tufts University School of Medicine, Guy Smagghe, University fo Ghent, Addy Alt-Holland, Tufts University, Jeffrey Kwak, Midwestern University, and Shruti Pore, Tufts University School of Dental Medicine
3:30 pm – 5:00 pm | Animal Contributed Paper Competition | Ballroom C |
To support the Society’s vision to encourage education and scientific informational exchange and recognize outstanding students and post docs, the In Vitro Animal Cell Sciences section is pleased to announce the 2013 In Vitro Animal Cell Science Research Oral Presentation Competitions for both Students and Post Docs. Students and Post Docs wishing to enter submitted a copy of their title and abstract (as submitted to SIVB) and abstract tracking number to Dr. Addy Alt-Holland. Only those abstracts formally submitted to both SIVB and Dr. Alt-Holland were considered. Finalists were selected based on the quality of the abstracts. A panel of judges will evaluate the presentations at the meeting. Criteria are experimental design, data analysis, proper interpretation of the results, originality of the study, technical difficulty, appearance of the presentation and ability of the finalist to respond to questions. Winners will be presented with a certificate and a cash award at the meeting.
1:30 | A-1000 | Development of Cell Lines from the Walleye Caudal Fin and Their Ability to Survive and Support Viral Hemorrhagic Septicemia Virus (VHSV) Group IVb Replication at Low Temperatures Aaron W. Bender, University of Waterloo, N. T. K. Vo, P. A. L. Rivers, J. S. Lumsden, B. Dixon, and N. C. Bols |
1:50 | A-1001 | The Susceptibility of Walleye Cell Lines from the Spleen Stroma and Caudal Fin to Viral Hemorrhagic Septicemia Virus (VHSV) Nguyen T. K. Vo, University of Waterloo, Hina A. Bandukwala, Aaron W. Bender, John S. Lumsden, Lucy E. J. Lee, Brian Dixon, and Niels C. Bols |
2:10 | A-1002 | Effects of Differential Trk Expression on Neuroblastoma Cells Cultured in the Microgravity Rotary Bioreactor Robert A. Redden, The Children’s Hospital of Philadelphia, Jane E. Minturn, Garrett M. Brodeur, and Edward J. Doolin |
In Vitro Culture/Micropropagation
Moderator: Matthew Curtis, The Pennsylvania State University
1:30 pm – 2:30 pm | Plant Interactive Poster Session | Ballroom A |
P-2000 | Biosynthesis Enhancement of Stilbenoids in Hairy Root Cultures of Peanut L. Ogutu, Arkansas State University, L. Nopo-Olazabal, and F. Medina-Bolivar |
P=2001 | Novel Temporary Immersion Bioreactor Allows the Manipulation Headspace Composition to Improve Plant Tissue Propagation M. Curtis, The Pennsylvania State University, S. Florez, and W. Curtis |
P-2002 | Shoot Organogenesis from In Vitro Derived Leaf Tissues of Piper longum D. Rani, Dayalbagh Educational Institute (Deemed University), and P. K. Dantu |
P-2004 | Towards Automation of Micropropagation Using a Mist Bioreactor L. Fei, Worcester Polytechnic Institute, and P. J. Weathers |
Plant Biotechnology
Moderator: Liu Y. Shen, Dow AgroSciences
1:30 pm – 2:30 pm | Plant Interactive Poster Session | Ballroom A |
|
Haploids and Haploidization
Convener: Joseph F. Petolino, Dow AgroSciences
3:30 pm – 5:00 pm | Plant Symposium | Ballroom D |
Haploids are invaluable materials for basic genetic studies as well as for applied breeding and biotechnology. The instantaneous line fixation that accompanies the doubling of the chromosome comlement of haploids enhances the efficiency of inbred line development and selection. The totipotent microspore represents an attractive target for transgene delivery. Functional genomics, of polyploid species in particular, become more powerful with the availability of haploid production technology. This session will highlight some in vitro methods used to generate and employ haploids for crop improvement.
3:30 | Introduction (J. Petolino) | |
3:35 | P-16 | Haploids in Plant Breeding and Genomic Research Tatiana Boluarte-Medina, Virginia Tech |
4:00 | P-17 | Isolated Microspore Culture in Cereals by Mediating Stresses and Nursing Rakesh Sinha, Agriculture and Agri-Food Canada |
4:25 | P-18 | Improving Camelina Doubled Haploidy: Microspore Transformation Using Nanocarriers Alison Ferrie, Plant Biotechnology Institute |
4:50 | Discussion |
Non-competitive Student Oral Presentations
Conveners: Jessica L. Rupp, Kansas State University, and Kerri Neugebauer, Kansas State University
3:30 pm – 5:00 pm | Education Workshop | Room 556A/B |
Public speaking and presenting research findings are a large part of a scientific career. What better way to become proficient at oral presentations than to practice? If your abstract was not chosen for the competitive session, and you would still like an opportunity to present your research, this is the session for you! This new session will allow speakers to practice their public speaking skills in a relaxed, friendly environment without the pressure of being in a competition. All students, even those that don’t submit an abstract to the oral competition, are welcome to apply to speak at this session. To make this a learning experience for everyone involved, the speaker will receive constructive criticism from other members and students of SIVB by using the same judging rubric from the competitive session. Join us to practice speaking and share your research!
Participants:
A-2005 | Regulation of RNA Polymerase III Transcription by EGCG in Lungs Nicole Nole and Jana Suchtova, St. John’s University, and Laura Schramm |
P-2002 | Shoot Organogenesis from In Vitro Derived Leaf Tissues of Piper longum D. Rani, Dayalbagh Educational Institute (Deemed University), and P. K. Dantu |
P-2038 | Effect of Aluminum on Ex Vitro Rooting of Different Clones of Tectona grandis Lf Propagated In Vitro R. S. Barbosa, University of São Paulo, and A. N. Gonçalves |
P-2055 | Comparison of Larvicidal Effect of Rhizomes of Tissue Cultured and Naturally Grown Plants of Kaempferia galanga (Zengiberaceae) R. M. U. S. Senarath, Virgen Milagrosa University Foundation, Jimmy G. Catanes, and S. Senarath |
Rice Biotechnology
Conveners: Prakash P. Kumar, National University of Singapore, and Vibha Srivastava, University of Arkansas
3:30 pm – 5:00 pm | Plant Symposium | Ballroom B |
With about 140 million hectares annually under rice cultivation in Asia, rice is one of the most important staple food crops. Rice production ensures food security for more than half the world population. Rice-related industries have multibillion dollar annual turnover and it accounts for employing more people than any other single industry in Asia. Rice production is facing serious constraints especially due to global environmental changes. The productivity gains from the post-Green Revolution era of 1970s have attained saturation. Such yield stagnation is compounded by the declining arable land, water scarcity and global climate change. According to IRRI and the International Food Policy Research Institute USA, the price of rice will double by 2050 due to the combined effect of adverse climate changes on the crop and high demands for food grains due to population increase. Therefore, investments in rice research are essential to ensure and protect future rice supplies. Besides the R&D efforts of a number of agbiotech companies, initiatives such as the Global Rice Science Partnership (GRiSP) coordinated by the IRRI are attempting to address this. An integrated application of modern technologies including biotechnology for improving the crop is imperative in order to adapt the currently accepted varieties of rice to the changing environments and ensure stable rice supply.
3:30 | Introduction (P. P. Kumar and V. Srivastava) | |
3:35 | P-19 | Use of Metabolomics to Characterize a Dwarf Mutant Line of Rice Prakash P. Kumar, National University of Singapore |
4:00 | P-20 | A Field Validated and Versatile QTL for Drought Tolerance in Rice Ajay Kohli, International Rice Research Institute |
4:25 | P-21 | Biotechnological Approaches to Boost Rice Innate Immunity for Broad-Spectrum and Durable Disease Resistance Yinong Yang, Penn State University |
4:50 | Discussion |
Three Dimensional Tissues as Powerful Tools for Cancer Research
Conveners: Addy Alt-Holland, Tufts University, Michael Dame, University of Michigan Medical School, and Brad Upham, Michigan State University
3:30 pm – 5:00 pm | Animal Symposium | Ballroom C |
Modeling cancer development in bioengineered tissues, which mimic the features of their in vivo counterparts, enables cancer researchers to narrow the gap between experiments performed in monolayer cell cultures and in vivo. Significantly, three-dimensional tissue models and organ cultures allow for marinating dynamic and biologically meaningful interactions between different cell types, in a complex and supportive microenvironment. This symposium will focus on bioengineered, physiologically relevant, human skin tissues that consist of dermal and epidermal compartments, as well as on organ cultures from human skin biopsies. These tissues are used in the quest to reveal molecular mechanisms and signaling pathways that regulate the fundamental cellular processes of tumor cell motility and invasion. Novel therapeutic approaches designed to target these critical processes will potentially abrogate the progression of squamous cell carcinoma in the human skin.
3:30 | Introduction (A. Alt-Holland, M. Dame and B. L. Upham) | |
3:35 | A-10 | RalA: A Double Edged Sword in Skin Squamous Cell Carcinoma Adam G. Sowalsky, Harvard Medical School |
4:00 | A-11 | The Role of Dab2 in Directing the Pathogenesis of Human Skin Squamous Cell Carcinoma James Baleja, Tufts University School of Medicine |
4:25 | A-12 | Organ-cultured Human Skin to Study Squamous Epithelial Cell Invasion James Varani, The University of Michigan |
4:50 | Discussion |
Educational Kits Demonstration
Convener: Sylvia A. Mitchell, University of the West Indies
5:00 pm – 6:00 pm | Education Workshop | Room 556A/B |
This educational workshop will describe how to implement multiplex measurement of cell viability, necrosis, and apoptosis from the same sample of cells using a standard multimode plate reader. A detailed description of preparing cells for the assay, performing the sequential homogeneous add-mix-measure multiplex protocol, and analysis of example data will be presented. The advantages of using multiplexing as an internal control for cell-based assays will be described.
5:00 | Introduction (S.A. Mitchell) | |
5:05 | E-1 | Education Kit for Determining Cell Viability, Death and Apoptosis from the Same Sample Terry Riss, Promega |
5:50 | Discussion |
In Vitro Animal Cell Sciences Contributed Papers
Moderators: Brad L. Upham, Michigan State University, and Shruti Pore, Tufts University School of Dental Medicine
5:00 pm – 6:00 pm | Animal Contributed Paper Session | Ballroom C |
5:00 | A-1003 | Comparison of Human Colonic Crypts and Abnormal Crypts from Adenoma in Culture: A Multi-stage Model to Assess Colon Chemoprevention Y. Jiang, University of Michigan Medical School, M. K. Dame, D. K. Turgeon, H. Appelman, M. N. Aslam, K. Copley, D. Attili, D. Brenner, and J. Varani |
5:15 | A-1004 | Voluntary Exercise Prevents Obesity and Significantly Alters Major Gut Bacteria Phyla Jeff Kwak, Midwestern University, Christian C. Evans, Samantha Laskowski, Joseph Dougherty, Kathy J. LePard, and Mae J. Ciancio |
5:30 | A-1005 | The p53 Inhibitor, Pifithrin-µ , Induces Apoptosis in Rainbow Trout Gill Cell Line, RTgill-W1 F. Zeng, University of Waterloo, J. P. Sherry, B. Dixon, B. P. Duncker, N. C. Bols |
5:45 | A-1006 | Using Fish Cell Lines to Study How Engineered Nanomaterials Influence the Survival of Viruses from Lower Aquatic Vertebrates Y. J. Huang, University of Waterloo, P. H. Pham, A. Hu, K. Oakes, S. X. Tang, and N. C. Bols |
Plant Biotechnology Contributed Papers II
Moderator: Zhanyuan Zhang, University of Missouri
5:00 pm – 6:00 pm | Plant Contributed Paper Session | Ballroom B |
5:00 | P-1014 | Transcriptional Activity and Practical Use of Ubiquitin and PR-1 Gene Promoters from Grapevine (Vitis spp.) in Transgenic Plant Recovery D. J. Gray, University of Florida/IFAS, Z. T. Li, K.-H. Kim, J. R. Jasinski, D. D. Silva, and M. R. Creech |
5:15 | P-1015 | Hydroxyproline-O-Glycosylated Biopolymer Carriers for Competitive Plant Cell and Tissue Bioproduction Platforms Jianfeng Xu, Arkansas State University, Maureen Dolan, Ningning Zhang, and Marcia Kieliszewski |
5:30 | P-1016 | Synthetic TAL Effectors for Targeted Gene Activation in Plants C. Neal Stewart, Jr., The University of Tennessee, Wusheng Liu, Mary R. Rudis, Yanhui Peng, Mitra Mazarei, Reginald J. Millwood, Jian-Ping Yang, and Jonathan D. Chesnut |
5:45 | P-1017 | Somatic Embryogenesis from Immature Embryos for the Transformation and Regeneration of Sorghum Lines (Sorghum bicolor L.) Shireen K. Assem, Agricultural Genetic Engineering Research Institute, S. H. El-Mansy, M. M. Zamzam, and E. H. A. Hussein |
Powerpoint and Enhancements for Presentations
Facilitator: James J. Sadler, University of Florida
5:00 pm – 6:00 pm | Education Workshop | Ballroom D |
Presenters and teachers traditionally use presentation software to deliver lectures with supporting graphics. Over the past decade, PowerPoint has become the dominant software for teachers and researchers. However, other options are available that could provide a better vehicle for presenting information. Prezi, a new presentation program, will be introduced as an alternative to PowerPoint. Advantages and challenges, in addition to the potential uses, of Prezi will be discussed among the audience members to generate “etiquette” for using this program. Cloud presentation programs, such as GoogleDocs and SlideRocket, will be discussed including their ability to help multi-institutional development of presentations. Finally, PowerPoint presentation enhancements will be presented for those who want to continue using PowerPoint. In addition to presentations, alternatives and enhancements to posters will be presented and discussed.