Daily Program
Abstracts & Posters
Keynote Speaker Keynote Symposium Plant Contributed Papers
Sunday, June 3 International Conference Animal Posters
Monday, June 4 Animal Symposia & Workshops Education Posters
Tuesday, June 5 Plant Symposia & Workshops Plant Posters
Wednesday, June 6 Animal Contributed Papers Addendum Booklet
Thursday, June 7

Wednesday, June 6, 2012

Daily Program-at-a-Glance

Time Event Location
7:00 am – 5:00 pm Registration Evergreen Foyer
10:00 am – 3:30 pm Exhibits and Posters Evergreen DEFGHI
Morning
8:00 am – 12:00 pm
 
7:00 am – 8:00 am In Vitro – Animal Editorial Board Meeting Cottonwood
  Student Affairs Breakfast Regency EFG
8:00 am – 10:00 am Omics Technologies in the Dissection of Biological Processes Evergreen ABC
10:00 am – 10:30 am Coffee Break Evergreen DEFGHI
  Constitution and Bylaws Committee Meeting Cottonwood
  Closing of the Puget Sound Silent Auction Evergreen DEFGHI
10:30 am – 12:30 pm Carcinogenesis: The Need for Advanced Mechanistic In Vitro Systems Executive Auditorium
  In Vitro Methods for Crop Improvement Regency ABCD
  Teaching Concepts in Transgenic Crops Evergreen ABC
Afternoon
12:00 pm – 5:00 pm
 
12:30 pm Announcement of the Puget Sound Silent Auction Winners Evergreen Foyer
12:30 pm – 1:30 pm 2013 Program Planning Committee Meeting Cottonwood
  Development Committee Meeting Juniper
1:30 pm – 2:30 pm Interactive Poster Sessions  
  Biotechnology Evergreen DEFGHI
  Plant Transformation Evergreen DEFGHI
  In Vitro Tools for Human Cancer Research Executive Auditorium
2:30 pm – 3:30 pm Poster Session
Odd Poster Authors will be present
Evergreen DEFGHI
2:30 pm – 3:45 pm Regulatory Acceptance of Alternative Carcinogenicity Tests Executive Auditorium
3:30 pm – 5:00 pm Poster Breakdown and Removal Evergreen DEFGHI
  Applications of RNAi and MicroRNA in Agriculture Evergreen ABC
  Inducible and Tissue-specific Plant Gene Expression Systems Regency ABCD
3:45 pm – 5:00 pm New Therapeutic Approaches to Cancer from In Vitro Studies Executive Auditorium
Evening
5:00 pm – 11:00 pm
 
5:00 pm – 5:45 pm SIVB Business Meeting (All Members Are Urged to Attend)
Student Award Presentations
Evergreen ABC
6:30 pm – 10:00 pm An Evening at Chateau Ste. Michelle Winery
Admittance by Advance Ticket Holders Only
Chateau Ste. Michelle

Wednesday, June 6

7:00 am – 5:00 pm Registration Evergreen Foyer

OMICS TECHNOLOGIES IN THE DISSECTION OF BIOLOGICAL PROCESSES

Conveners: Patrick McNutt, USAMRICD, and Andy Pereira, University of Arkansas

8:00 am – 10:00 am Plenary Symposium Evergreen ABC

Plant and animal biology is reaching new vistas with the advancement of “-omics” technologies and subsequent exponential generation of high-throughput data. The -omics information especially in genomics and transcriptomics is being driven by novel and inexpensive methods of next generation sequencing, where methods developed for personalized human medicine are contributing to sequence based analysis of specific genotypes and gene expression atlases of tissue and cell types. DNA sequencing methods differ in the sequence length and accuracy, competing for cost-reduction and more information. This symposium will provide a view of developments in omics technologies and how they are providing new strategies to compare plant and animal genomes and map genotypes, as well as chart gene expression patterns in developmental and environmental response.

8:00   Introduction (P. McNutt and A. Pereira)
8:05
PS-8
Genomics Approaches for Biochemical Pathway Discovery in Medicinal Plant Species
Robin Buell, Michigan State University
8:40
PS-9
Omics Technologies to Tap Genetic Variation for Rice Improvement
Jan Leach, Colorado State University
9:15
PS-10
TBA
9:50   Discussion

10:00 am – 10:30 am Coffee Break Evergreen DEFGHI

CARCINOGENESIS: THE NEED FOR ADVANCED
MECHANISTIC IN VITRO SYSTEMS

Conveners: Brad L. Upham, Michigan State University, Michael K. Dame, University of Michigan, and Addy Alt-Holland, Tufts University

10:30 am – 12:30 pm Animal Symposium Executive Auditorium

Cancer encompasses more than 200 different diseases, and because of its complexity, progress against certain cancers has been difficult. Nevertheless, cancer research has made major strides in identifying the underlying mechanisms of this disease. The accumulated knowledge is enabling our understanding of cancer at the molecular, cellular and tissue levels, and is leading to new diagnostic and treatment strategies that hold promise for increased clinical efficacy and survival. In vitro culture systems have proved invaluable in these efforts and are extensively used for uncovering the mysteries of cancer initiation and progression. Previously, the study of tumorigenesis has been significantly impaired by limitations inherent in two-dimensional cell culture systems that sharply reduce complexity. In contrast, heterogonous three-dimensional (3D) co-cultures more realistically model the cellular and structural architecture, the tumor microenvironment and the differentiated function of human cancers. Due to their enormous potential, 3D tissues provide reliable and credible tumor models for therapeutically oriented studies. This symposium will highlight some of the significant advances in 3D tissue culture techniques, and the development of increasingly more complex in vitro 3D co-culture tumor model systems for cancer research and drug development.

10:30   Introduction (B. L. Upham, M. K. Dame, and A. Alt-Holland)
10:35
A-15
Tubeless Microfluidic Systems for Personalized Chemotherapy
Albert Folch, University of Washington
11:10
A-16
Telomerase Tales from the 3rd Dimension
Shibani Mukherjee, Fred Hutchinson Cancer Research Center
11:45
A-17
3D Natural Polymer-based Matrices for Cancer Research and Drug Screening
Miqin Zhang, University of Washington
12:20   Discussion
 

IN VITRO METHODS FOR CROP IMPROVEMENT

Convener: Joseph F. Petolino, Dow Agrosciences

10:30 am – 12:30 pm Plant Symposium Regency ABCD

The culture of plant cells and tissues in vitro has become a foundational capability for crop improvement. The rather remarkable ability of the smallest fragment of plant tissue to recapitulate an entire organism – manifested in the concept of cellular totipotency – is at the heart of plant biotechnology. For virtually every crop species currently amenable to modern biotechnological manipulation, advances in plant cell and tissue culture were prerequisite. Being able to reduce complex, multicellular organisms, such as higher plants, down to the cellular level, manipulate them genetically and then regenerate back whole plants, has opened up several intriguing possibilities for crop improvement. This session will focus on the development and application of various in vitro methods aimed at improving major crop species.

10:30   Introduction (J. Petolino)
10:35
P-19
Maize Protoplast-based Transient Assay System for Analysis of Gene Functionality and Controlling Sequences
Paul Miller, Dow AgroSciences
11:00
P-20
The Use of Protoplasts for Citrus Improvement
Manjul Dutt, University of Florida
11:25
P-21
In Vitro Chemical Mutagenesis for Improvement of Turf Quality of Bahiagrass
Fredy Altpeter, University of Florida 
11:50
P-22
Oil Modification via Transcriptional Activation of Canola KASII Using an Engineered Zinc Finger Transcription Factor
Joseph F. Petolino, Dow AgroSciences
12:15   Discussion
 

TEACHING CONCEPTS IN TRANSGENIC CROPS

Convener: Kevin M. Folta, University of Florida

10:30 am – 12:30 pm Plant Workshop Evergreen ABC

BIOTECHNOLOGY

Moderator: Baochun Li, Bayer CropScience

1:30 pm – 2:30 pm Plant Interactive Poster Session Evergreen DEFGHI

P-2008
Expression of Anthocyanin MYB Autoregulatory Transcription Factors in Petunia
Murray R. Boase, The New Zealand Institute for Plant & Food Research Limited, Cyril Brendolise, Kathy E. Schwinn, Kevin M. Davies, Richard V. Espley, and Roger P. Hellens
P-2009
Bioreactor Design for In Vitro Plant Propagation and Somatic Embryo Induction Using Transient Expression of Transcription Factors
Sergio Florez, Penn State University, Sydney Shaw, and Wayne R. Curtis
P-2011
Characterisation of the Pistacia Genus via Retrotransposon Based Marker Systems
Emrah Kirdok, Gebze Institute of Technology, and Y. Özden Çiftçi
 

IN VITRO TOOLS FOR HUMAN CANCER RESEARCH

Moderator: Addy Alt-Holland, Tufts University

1:30 pm – 2:30 pm Animal Contributed Paper Session Executive Auditorium

1:30
A-1006
Integrating Omics Data for the Better Understanding of Cancer
Hongxing Lei, Chinese Academy of Sciences, Yuyun Pan, Yuchao Pei, and Jiajia Wang
1:45
A-1007
Cullin-5 Knockdown Alters Protein Expression in MDA-MB-231 Breast Cancer Cells
Jeff W. Kwak, Midwestern University, L. A. C. Alt, and M. J. Fay
2:00
A-1008
Human Colon Mucosal Crypts and Epithelial Cells Isolated in Culture: A Model to Access Differentiation and Proliferation in the Epithelial Component
Michael K. Dame, University of Michigan Medical School, Y. Jiang, K. Copley, D. Brenner, and J. Varani
2:15
A-1009
E-cadherin Loss Drives Squamous Cell Carcinoma Tumor Cell Motility and Invasion Through Altered Expression and Localization of Dab2 and Beta 1 Integrins
Addy Alt-Holland, Tufts University, S. Pore, J. Sun, A. Maione, J. Garlick, and J. Baleja
 

PLANT TRANSFORMATION

Moderator: M. Aydin Akbudak, University of Arkansas

1:30 pm – 2:30 pm Plant Interactive Poster Session Evergreen DEFGHI

P-2012
Progress Toward Castor (Ricinus communis L.) Transformation and Silencing the Ricin Gene
D. J. Barnes, Mississippi State University, N. A. Reichert, and B. S. Baldwin
P-2013
RNAi Mediated Silencing of Hydroxycinnamoyl-CoA Quinate-hydroxycinnamoyl Transferase alters Phenylpropanoid Metabolism in Potato
R. S. Payyavula,Washington State University, D. A. Navarre
P-2014
Fungal Mitochondrial DNases: an Unlimited Source of Genes for Engineering Disease Resistance in Plants
Lee A. Hadwiger, Washington State University
P-2015
Effect of Various Sterilization Procedures on the In Vitro Germination of Cotton Seeds Collected from the Open Field
Sergei Krasnyanski, North Carolina State University, S. Barampuram, C. Haigler, and G. Allen
 

Wednesday, June 6
Odd Poster Authors will be present
2:30 pm – 3:30 pm

REGULATORY ACCEPTANCE OF ALTERNATIVE CARCINOGENICITY TESTS

Japanese Society for Alternatives to Animal Experiments
(JSAAE) Joint Session

Convener: Hajime Kojima, Japanese Society for Alternatives to Animal Experiments

2:30 pm – 3:45 pm Animal Symposium Executive Auditorium

In the OECD working plan four cell transformation assays (SHE pH 6.7, SHE pH 7, Balb/c 3T3 and Bhas assays) are being evaluated to establish test guidelines for screening carcinogens. To date pre-validation studies on three cell transformation assays (SHE pH 6.7, SHE pH 7, and Balb/c 3T3) were completed by ECVAM (European Center for the Validation of Alternative Methods) and reviewed by ESAC (ECVAM Scientific Advisory Committee). The validation of the Bhas assay was completed by JaCVAM (Japanese Center for the Validation of Alternative Methods). In this session updates of these assays will be presented by two speakers, and future directions will be discussed.

2:30
  Introduction (H. Kojima)
2:35
A-18
Cell Transformation Assays: The ECVAM Study and Follow-up Activites
Raffaella Corvi, European Center for the Validation of Alternative Methods
3:00
A-19
Bhas 42 Cell Transformation Assay for the Prediction of Chemical Carcinogenicity
Ayako Sakai, Drug Safety Center, Kanagawa
3:25
A-20
OECD Activities on the Cell Transformation Assays
Hajime Kojima, JaCVAM, NIHS, Japan
3:35
  Discussion
 

APPLICATIONS OF RNAI AND MICRORNA IN AGRICULTURE

Convener: Harold N. Trick, Kansas State University, and John McMillan, BASF Plant Science

3:30 pm – 5:00 pm Plant Symposium Evergreen ABC
Our knowledge of the molecular mechanisms of gene regulation through silencing has increased dramatically in the last few years. In addition the “classical” RNA interference technology, alternative silencing methods such as micro RNAs and transacting or tasiRNA have been discovered. In the last several years gene silencing has also become increasingly important in agriculture as a method to test gene functionality and as a method for crop improvement. In this session we will cover these different methods currently used for gene silencing, specific applications and future potential of this technology in agriculture.

3:30
  Introduction (H. N. Trick and J. McMillan)
3:35
P-23
RNA Interference and Plant Biotechnology
John McMillan, BASF Plant Science
4:00
P- 24
Next Generation Gene Silencing Vectors in Soybean
Thomas Jacobs, University of Georgia
4:25
P- 25
Overexpression of miR156 for Switchgrass Improvement
Zeng-Yu Wang, The Samuel Noble Roberts Foundation
4:50
  Discussion
 

INDUCIBLE AND TISSUE-SPECIFIC PLANT
GENE EXPRESSION SYSTEMS

Conveners: Mary Welter, Dow AgroSciences and Roger Thilmony, USDA-ARS

3:30 pm – 5:00 pm Plant Symposium Regency ABCD

Until recently, the expression of recombinant proteins has typically been controlled by strong constitutive promoters.  As it became apparent that constitutive expression can be unstable or deleterious to the host organism, the priority in promoter development has shifted to those that drive transgene expression in a temporal, spatial or developmentally-specific manner. This session will present several options in plant transgene expression systems.

3:30
  Introduction (M. Welter and R. Thilmony)
3:35
P-26
An Inducible Three-component Gene Expression System and Its Application for Inducible Flavonoid Overproduction in Transgenic Arabidopsis Thaliana
Hisashi Koiwa, Texas A&M University
3:55
P-27
Evolution of the Tetracycline Repressor into a Sulfonylurea Herbicide Responsive Gene Switch for Crop Plants
Kevin McBride, Pioneer Hi-Bred International
4:15
P-28
Genetic and Epigenetic Beauty, Ugliness and Complexity of Flower-specific Promoters and Enhancers
Zongrang Liu, USDA-ARS
4:35
P-29
Leaf-, Root- and Pollen-specific Promoters from Rice; Useful New Tools for Improved Crop Biotechnology
Roger Thilmony, USDA-ARS
4:55
  Discussion

NEW THERAPEUTIC APPROACHES TO
CANCER FROM IN VITRO STUDIES

The Japanese Tissue Culture Association (JTCA) Joint Session

Conveners: Miho Furue, National Institute of Biomedical Innovation, and Ken Kataoka, Okayama University

3:45 pm – 5:00 pm Animal Symposium Executive Auditorium

Cancer cells, like normal cells, are not free from environmental stresses including inflammatory, endoplasmic reticulum (ER) and oxidative stresses. It is known that inflammation contributes to tumor growth, progression, and metastasis. During tumorigenesis, high proliferation rates of cancer cells require increased activities of ER protein folding, assembly, and transport, a condition that can induce ER stress. Conditions of oxidative stress play an important role in both the initiation and the progression of cancer. Cancer cells must balance these stresses to survive. In this session the JTCA speakers will report on new therapeutic approaches to cancer based on the controlling these stresses from in vitro studies.

3:45 A-21 Introduction: New Therapeutic Approaches to Cancer from In Vitro Studies
Ken Kataoka, Okayama University
3:55 A-22 Selective Cancer Cell Killing by Ashwagandha Leaf Extract Involves Activation of p53 and Oxidative Stress Signaling: Evidence from Cell-based Loss-of-Function  Screenings
Renu Wadwa, National Institute of Advanced Industrial Science & Technology
4:15 A-23 Preparation and Culture of Spheroids Composed of Pure Primary Cancer Cells from Patients’ Tumor Samples
Masahiro Inoue, Osaka Medical Center for Cancer and Cardiovascular Disease
4:35 A-24 A Novel Tumor Suppressor, REIC/Dkk-3 Gene Identified by Our In Vitro Transformation Model of Normal Human Fibroblasts Works as a Potent Therapeutic Anti-tumor Agent
Masakiyo Sakaguchi, Okayama University
4:55   Discussion
 
5:00 pm – 5:45 pm

SIVB Business Meeting
(All Members Are Urged to Attend)
Student Award Presentations

Evergreen ABC
6:30 pm – 10:00 pm
An Evening at Chateau Ste. Michelle Winery
Admittance by Advance Ticket Holders Only
Chateau Ste. Michelle