Tuesday, June 5, 2012

Daily Program-at-a-Glance

Time	Event	Location
7:00 am – 6:00 pm	Registration	Evergreen Foyer
10:00 am – 3:30 pm	Exhibits and Posters	Evergreen DEFGHI
Morning	8:00 am – 12:00 pm
7:00 am – 8:00 am	Nominating Committee Meeting	Cottonwood
	Publications Committee Meeting	Juniper
8:00 am – 10:00 am	Nutraceuticals in the 21st Century: Identification, Production, and Demonstration of Efficacy and Safety	Evergreen ABC
10:00 am – 10:30 am	Coffee Break	Evergreen DEFGHI
	Education Committee Meeting	Cottonwood
10:30 am – 11:45 am	Plant Biotechnology Contributed Papers I	Evergreen ABC
10:30 am – 12:30 pm	Identifying Environmental Endocrine Disruptors: Do In Vitro Models Predict Relevance?	Executive Auditorium
	Next Gen Transformation Technologies	Regency ABCD
Afternoon	12:00 pm – 5:00 pm
12:30 pm – 1:30 pm	Membership Committee Meeting	Cottonwood
	Refreshment Break	Evergreen DEFGHI
12:30 pm – 2:00 pm	Student Networking Luncheon	Regency EFG
1:30 pm – 2:30 pm	Interactive Poster Sessions
	Embryogenesis/Micropropagation/ Regeneration	Evergreen DEFGHI
	Genomes/Genomics/Bioinformatics	Evergreen DEFGHI
	In Vitro Tools, Techniques, and Optimization	Evergreen DEFGHI
2:30 pm – 3:30 pm	Poster Session Even Poster Authors will be present	Evergreen DEFGHI
3:30pm – 5:00 pm	Advanced 3-D Tissue Culture Systems	Executive Auditorium
	DNA Recombination Based Tools for Plant Genetic Engineering	Evergreen ABC
	In Vitro Tools for Plant Conservation	Regency ABCD
Evening	5:00 pm – 11:00 pm
5:00 pm – 6:00 pm	Gene Knockdown and Gene Knockout	Executive Auditorium
	Research Integrity	Regency ABCD
	Plant Biotechnology Contributed Papers II	Evergreen ABC
6:00 pm – 7:30 pm	In Vitro Animal Cell Sciences Section Business Meeting	Executive Auditorium
	Plant Biotechnology Section Business Meeting	Regency ABCD
7:30 pm – 10:00 pm	SIVB Sections’ Social	Cedar Ballroom and Cedar Ballroom Foyer

Tuesday, June 5

7:00 am – 6:00 pm Registration Evergreen Foyer

NUTRACEUTICALS IN THE 21ST CENTURY: IDENTIFICATION, PRODUCTION, AND DEMONSTRATION OF EFFICACY AND SAFETY

Conveners: Mary Ann Lila, North Carolina State University, and John W. Harbell,
Mary Kay Inc.

8:00 am – 10:00 am Plenary Symposium Evergreen ABC

Traditional medicine has relied on indigenous plants and other natural products to address a wide range of human needs (nutrition and medicinal). Through the empirical process of biodiscovery, wildcrafted plants were identified with the capacity to prevent or provide therapy for chronic human diseases, heal wounds, or, to bolster metabolism. In a great many cases, the traditional discoveries have a very strong pharmacological basis and this has attracted interest from academic and commercial entities. Already, a large number of bioactive botanical-based ingredients, drawn from traditional medicine, are found in nutritional supplements, nutraceuticals and cosmetics. Bringing a traditional medical approach to commercialization as a nutraceutical or “active ingredient” faces many challenges. These include, identifying promising technology from the array of traditional practices, sourcing the botanical material, determining the active constituents, measuring their potency across lots and establishing the legal right to use the technology (bio-sustainability and the Nagoya Protocol). This symposium will focus on the application of in vitro approaches to addressing sourcing of the active molecules, efficacy and potency testing, and toxicology/safety testing.

8:00		Introduction (M. A. Lila and J. W. Harbell)
8:05	PS-5	Botanical Ingredients in Medicinal, Nutritional and Cosmetic Applications: Sourcing, Quality and Safety Issues *Larry Walker,* The University of Mississippi
8:40	PS-6	The Use of In Vitro GI Models to Estimate Food Matrix Effects on Phytochemcial Bioavailability *Mario G. Ferruzzi,* Purdue University
9:15	PS-7	Skin Regenerating Active Produced from Rosa Dedifferentiated Cell Culture *Barbara Schmidt,* L’Oreal USA
9:50		Discussion

10:00 am – 10:30 am Coffee Break Evergreen DEFGHI

IDENTIFYING ENVIRONMENTAL ENDOCRINE DISRUPTORS: DO IN VITRO MODELS PREDICT RELEVANCE?

Conveners: Eugene Elmore, University of California – Irvine, Warren Casey, NICEATM, NIEHS, and Lyle D. Burgoon, US EPA

10:30 am – 12:30 pm Animal Symposium Executive Auditorium

The endocrine disruptor program began with the formal announcement in 2000 of the U.S. Environmental Protection Agency’s effort to validate various in vitro test methods for identifying agents with the potential to modify or interfere with the function of various hormones in the endocrine system. Subsequent validation efforts have demonstrated that estrogen and androgen agonists and antagonists can be detected in vitro. This session will overview various endocrine disruptor screening assays, including novel approaches and will include practical applications for the environment. Examples will be presented to illustrate how endocrine disruptor activity can be detected using a variety of technologies. The screening assays utilize human cells with natural or engineered receptors and/or response elements to facilitate detection. The lack of sufficient numbers of reference chemicals that have consensus agreement regarding activity (or lack thereof) for the various endpoints has lead to challenges in data interpretation. The validation process has demonstrated a spectrum of activity with most agents showing some activity in one or more of the assays. However, the absence of a unified methodology for assessing activity (e.g. Positive/Negative, calculation of potency and/or efficacy) is complicating the analysis and acceptance of data. Once issues of data analysis are addressed, the ultimate challenge will be interpreting data in terms of exposure that results in functional disruption of the endocrine system. The assays that have been validated for use in ED screening programs (get references prior to publishing) are generally classified as low throughput. High throughput methods which are capable of generating many thousands of measurement per day are currently being explored (i.e., US EPA’s ToxCast and Tox21) and are thought by many to be the answer to solving the problem of interpretation by providing insight on multiple “toxicity pathways”. Although these methods do generate a tremendous amount of data, the analysis and interpretation of the data, in terms of metazoan physiology, remains to be demonstrated. Much effort has been put into developing low and high throughput methods to detect in vivo activity of chemicals that may cause adverse changes in the endocrine system of humans and animals. The challenge now is not so much in generating data, but reaching consensus on how to analyze and interpret these data in terms of protecting public health and the environment. This session will overview the current status of the endocrine disruptor screening assays and will include validated and new in vitro methods as well as high-through put promoter assays to identify possible endocrine disruptor chemicals.

10:30		Introduction (E. Elmore, W. Casey, and L. D. Burgoon)
10:35	A-8	Utilization of Direct Receptor Visualization for Endocrine Activity Tests in Non-targeted Environmental and Chemical Screening *Raymond A. Lewis,* Cellomics, Thermo Fisher Scientific
11:05	A-9	Stably Transfected Estrogen Receptor Alpha Transactivation Assay Using HeLa9903 Cell Line as In Vitro Method to Screen the Endocrine Disruption Potentials of Chemicals *Atsushi Ono,* NIHS, Japan
11:35	A-10	EDSP21: The Incorporation of In Silico Models and In Vitro High Throughput Assays in the Endocrine Disruptor Screening Program (EDSP) for Prioritization and Screening *Scott G. Lynn,* US EPA
12:05	A-11	Validating High-throughput Test Methods for Tox21; the Technology Has Changed, but the Objective Remains the Same *Warren Casey,* NICEATM, NTP

NEXT GEN TRANSFORMATION TECHNOLOGIES

Conveners: Ajith Anand, Pioneer Hi-Bred International, Inc., and Delene Oldenburg, University of Washington

10:30 am – 12:30 pm Plant Symposium Regency ABCD

Since the introduction of the first plant biotechnology trait with application in 1995, the last fifteen years has seen an increase in traits and types of transgenic plants. Primarily, this was achieved by improvements in the transformation technologies such as direct DNA uptake, particle bombardment or Agrobacterium-mediated plant transformation. Agrobacterium-mediated plant transformation is the preferred method for commercial biotech product development, in part because of its simplicity and propensity to produce cleaner events. There are, however, specific areas such as variable-copy insertions, presence of backbone and random integration of the transgenes that needs to be addressed in a timely fashion. Agro-mediated transformation and T-DNA vectors are still important tools for plant genetic engineering, and recent advances will be highlighted. This session will also bring forth an assortment of newer tools currently being developed and deployed for improved transgene integration and trait development. Advances made in the areas of targeted gene delivery, co-operative transformation of organisms, and organelle transformation will be highlighted by eminent speakers from the academia and industry.

10:30		Introduction (A. Anand and D. Oldenburg)
10:35	P-8	Increasing Agrobacterium-mediated Genetic Transformation by Manipulating the Plant Genome *Stanton Gelvin,* Purdue University
11:05	P-9	Site-specific Integration and Trait Stacking in Corn; Implications for Transformation *Christopher Scelonge,* Dupont Agricultural Biotechnology
11:30	P-10	Endophytes for Enhanced Plant Growth, Pollutant Degradation, and Biofuels *Sharon L. Doty,* University of Washington
11:55	P-11	Overview of Next Generation Transformation Technology and Vector Design to Improve Plastid Transformation Efficiency *Delene Oldenburg,* University of Washington
12:20		Discussion

PLANT BIOTECHNOLOGY CONTRIBUTED PAPERS I

Moderator: John J. Finer, The Ohio State University

10:30 am – 11:45 am Plant Contributed Paper Session Evergreen ABC

10:30	P-1010	Development of P1 Gene Constructs to Confer Broad, Effective and Durable Resistance Against Rice Yellow Mottle Virus Olalekan Banwo, Ahmadu Bello University, Martina Paape, and Stephan Winter
10:45	P-1011	Micro-RNA Regulation for Boron Toxicity Tolerance in Barley *Turgay Unver,* Cankiri Karatekin University, Emine Gulden Erkilic, Vahap Eldem, Serdal Sakcali, Arif Ipek, Serkan Uranbey, and Sebahattin Ozcan
11:00	P-1012	Biotechnological Strategies for Conservation and RAPD Analysis of an Endangered Forest Tree Wrightia tinctoria– Important in Toy Making Industry. *Ramaswamy Nanna*, Kakatiya University, and Madhusudhan Kairamkonda
11:15	P-1013	Micropropagation of Native Plants for the Desert Rehabilitation Program in Kuwait *Chellan Sudhersan,* Kuwait Institute for Scientific Research, A. M. Al-Dousari, J. Ashkanani, and S. Al-Melhem
11:30	P-2045	Screening and Quantification of an Antiseptic Alkylamide, Spilanthol from In Vitro Cell and Tissue Cultures of Spilanthes Acmella Murr *Rakhi Chaturvedi,* Indian Institute of Technology, and Mithilesh Singh

STUDENT NETWORKING LUNCHEON

Conveners: Vinita Daniel, Midwestern University, and Kerri Neugebauer, Kansas State University

12:30 pm – 2:00 pm Education Workshop Regency EFG

Networking is a vital part of a student’s career. Relationships and connections established while perusing degrees will help to further advance student’s careers. The formation of strong bonds between members of an organization is an ideal opportunity to start the networking process. As leaders and innovators in their field, the professionals of the Society for In Vitro Biology are in the forefront of the rapidly changing scientific profession. These members are going to shape the industry where students will spend their careers. Join us to hear what these professionals have to say about the changing needs of our world. Meet and talk with other students and SIVB members to discuss the latest trends in science with leaders in the field in this relaxed roundtable session.

Panelists:

Mae Cianco, Midwestern University
Michael J. Fay, Midwestern University
Ted Klein, Pioneer Crop Genetics
Baochun Li, Bayer CropScience
Sylvia Mitchell, University of West Indies
Joe Petolino, Dow Agrosciences
T. Michael Spencer, Monsanto Company

EMBRYOGENESIS/MICROPROPAGATION/REGENERATION

Moderator: Soumen Nandy, University of Arkansas

1:30 pm – 2:30 pm Plant Interactive Poster Session Evergreen DEFGHI

P-2000	Micropropagation and Transformation of Rhizomatous Plant Species Ruifeng He, Washington State University, Min-Jeong Kim, and David R. Gang
P-2001	Micropropagation and Large-scale Multiplication of Stevia rabaudiana-Bio-sweetener of the Future A. Sabitha Rani, Osmania University College for Women, M. Satyakala, and V. Nagamani
P-2002	In Vitro Screening Protocols for Assessing Drought and Salinity Tolerance in Poplar (Populus spp.) Germplasm R. Ray, California State University, and J. T. Bushoven
P-2003	Prevention of Shoot Tip Necrosis Responses in In Vitro-proliferated Mature Pistachio Plantlets H. Akdemir, Gebze Institute of Technology, H. Yıldırım, E. Tilkat, A. Onay, and Y. Özden Çiftçi
P-2010	Metabolite Production in Plant Cell Cultures of Burdock (Arctium sp.) Bizhen Hu, The Ohio State University, Joseph C. Scheerens, John Cardina, and John J. Finer

GENOMES/GENOMICS/BIOINFORMATICS

Moderator: Lisa Lee, The Scotts Miracle Gro Company

1:30 pm – 2:30 pm Plant Interactive Poster Session Evergreen DEFGHI

P-2004	Sequencing Coconut (Cocos nucifera L.)Expressed Sequenced Tags (EST) Using 454 Life Sciences Technology H. D. D. Bandupriya, University of Reading, and J. M. Dunwell
P-2005	Migration of Organellar DNA into the Nucleus of Maize Rachana A. Kumar, University of Washington, D. J. Oldenburg, and A. J. Bendich
P-2006	Genetic Diversity of NBS-LRR Class Disease Resistance Gene Analogs in Cultivated and Wild Eggplants Y. Zhuang, Jiangsu Academy of Agricultural Sciences , X. H. Zhou, J. Liu, J. Jiang, and S. B. Wang
P-2007	Factors that Affect the Recovery of Taxus Transgenic Cell Lines Joyce Van Eck, University of Massachusetts at Amherst, P. Keen, S. Wilson, and S. Roberts

IN VITRO TOOLS, TECHNIQUES, AND OPTIMIZATION

Moderator: Michael K. Dame, University of Michigan

1:30 pm – 2:30 pm Animal Interactive Poster Session Evergreen DEFGHI

A-2000	Cell Matrix Interactions in 3D Alginate Cell Culture System Therese Andersen,FMC BioPolymer/NovaMatrix Industriveien, Christine Markussen, and Michael Dornish
A-2001	Increased Viability of Encapsulated Cells for Sensing Applications B. C. Butler, Luna Innovations, Inc., K. Moler, H. Roach, K. Dierksen, and J. Trempy
A-2002	Prospect of Neural Cells Derived from Human Pluripotent Stem Cells for Application of In Vitro Developmental Toxicity Test *Miho Kusuda Furue,* National Institute of Biomedical Innovation, Sumiyo Mimura, Mika Suga, Masaki Kinehara, Daiki Tateyama, Mitsuhi Hirata, Hiroki Nikawa, and Kana Yanagihara
A-2003	A Novel Molecular Tool Box for Fish Cell Analyses Marina Gebert, Fraunhofer Research Institution for Marine Biotechnology, Julia Kirchhof, Sebastian Rakers, and Charli Kruse
A-2004	Selectively Expressed Heat Shock Protein 70 Prevents Diet-induced Changes in Pancreatic Structure and Function. Mae Ciancio, Midwestern University, S. Duminie, M. Manalastas, C. Evans, and K. LePard
A-2005	Applications of Fish Cell Culturing To Conservation Biology: A Look at Oreochromis esculentus Cell Cultures for Genome Resource Banks. *Melissa Filice,*Toronto Zoo, and Gabriela F. Mastromonaco
A-2006	Cryopreservation of Human Epidermal Skin Models Lia H. Campbell, Cell & Tissue Systems, Inc,. Z. Chen, H. Kershaw, and K. G. M. Brockbank

Tuesday, June 5
Even Poster Authors will be present
2:30 pm – 3:30 pm

ADVANCED 3-D TISSUE CULTURE SYSTEMS
Conveners: Addy Alt-Holland, Tufts University, Michael K. Dame, University of Michigan, and Brad L. Upham, Michigan State University

3:30 pm – 5:00 pm Animal Workshop Executive Auditorium

DNA RECOMBINATION BASED TOOLS FOR PLANT GENETIC ENGINEERING

Conveners: James Thomson, USDA and Hong Luo, Clemson University

3:30 pm – 5:00 pm Plant Symposium Evergreen ABC

Plant genetic modification and improvement using recombinant DNA- and transgenic based biotechnology approaches become increasingly important for modern agriculture. GM products with modified characteristics have been widely accepted contributing significantly to agriculture production. Effective trait modification of important crop species for enhanced performance calls for development of novel technologies of plant genetic engineering. New strategies developed for gene targeting to specific genetic locus and for simultaneous transfer of multiple genes into plant as well as for transgene containment are among the most important breakthroughs that would dramatically impact the efficacy and effectiveness of plant genetic modification. Recent progress in studying site-specific recombination systems for use in plants has opened the door for innovative approaches of plant genome modifications. Site-specific DNA recombinases are enzymes that recognize specific DNA sequences, and depending on how the two recombination target sites are located they can catalyze site-specific recombination of DNA strands for excision, inversion or integration. With examples, speakers in this session will explore the use of site-specific DNA recombination systems in developing novel strategies for gene targeting, gene stacking and gene containment as well as other important aspects of plant genetic engineering.

3:30		Introduction (J. Thomson and H. Luo)
3:35	P-12	Development of Site-specific Recombinase Technology for Precise Crop Plant Genome Modification *James Thomson,* USDA-ARS
3:55	P-13	An Integrated Dual Recombination System for Use in Producing Clean Transgenic Plants *Hong Luo,* Clemson University
4:15	P-14	Plant Genome Editing: Knockout, Gene Stacking and Targeting *L. Alexander Lyznik,* Pioneer Hi-Bred International
4:35	P-15	Application of FLP-FRT System for Site-specific Gene Integration in Rice *Vibha Srivastava*, University of Arkansas
4:55		Discussion

IN VITRO TOOLS FOR PLANT CONSERVATION

Convener: Valerie C. Pence, Cincinnati Zoo & Botanical Garden

3:30 pm – 5:00 pm Plant Symposium Regency ABCD

Conserving plant biodiversity is a complex challenge requiring a variety of tools. While in situ conservation is of primary importance, current rates of habitat loss create a pressing need for ex situ propagation and germplasm preservation as a supplement and back-up to plants in the wild. In vitro techniques provide several methods that are being utilized in conservation efforts, including micropropagation and tissue cryopreservation. This session will provide examples of current research directed at maintaining and preserving endangered taxa, illustrating the potential and challenges of in vitro approaches.

3:30		Introduction (V. C. Pence)
3:35	P-16	In Vitro Conservation Tools in the Age of Extinction *Kingsley Dixon*, Kings Park and Botanic Garden
4:00	P-17	Ex Situ Plant Conservation in Global Biodiversity Hotspots and Island Countries: Importance of In Vitro Methods *Viswambharan Sarasan,* Royal Botanic Gardens Kew
4:25	P-18	Optimizing Cryopreservation Protocols for Plant Conservation and Restoration: The Genotypic Effect *James J. Sadler,* University of Florida
4:50		Discussion

GENE KNOCKDOWN AND GENE KNOCKOUT

Conveners: Michael J. Fay, Midwestern University, and Paul J. Price

5:00 pm – 6:00 pm Animal Symposium Executive Auditorium

The use of gene knockout and knockdown technology has become an invaluable research tool for examining gene function in both in vitro and in vivo experimental model systems. In this session participants will learn about the use of zinc-finger nuclease technology for creating gene knockouts. The session will also provide information concerning the approaches and reagents available for creating gene knockdowns using RNAi technology.

5:00		Introduction (M. J. Fay)
5:05	A-13	Efficient Genome Engineering with Zinc-finger Nucleases *Dana Carroll,* University of Utah School of Medicine
5:30	A-14	RNA Interference: A Tool to Elucidate Gene Function in Mammalian Cells *Annaleen Vermeulen,* Thermo Fisher Scientific
5:55		Discussion

RESEARCH INTEGRITY

Convener: Pamela J. Weathers, Worcester Polytechnic Institute

5:00 pm – 6:00 pm Plant Workshop Regency ABCD

The stakes of successful research are high. Publication of data in international, high quality journals can make the difference when it comes to obtaining a good job in a tight market, or getting a grant renewed or gaining acceptance into a prestigious lab. The emphasis to publish data in high-impact journals has resulted in pressure to commit falsification, fabrication, and plagiarism (FFP). This workshop will focus on best practices for performing science and being a successful scientist, with an emphasis on integrity and ethics. Beyond FFP, research integrity includes data management, peer review and confidentiality, authorship, collaboration, and conflicts of interest or commitment. Even the seemingly cut-and-dry topic of plagiarism has gray areas involving self-plagiarism and text recycling.The same technologies that facilitate FFP also make it easier to detect. Journal editors and reviewers have a duty to watch for and report instances of FFP. The workshop will not only inform the participant regarding research rules and best practices, but will also encourage critical thinking about the various gray areas that will enable successful, ethical science. Participants will receive syllabi currently used for training.

Panelists:

Todd Jones, Pioneer Hybrid
C. Neal Stewart, University of Tennessee
Pamela J. Weathers, Worcester Polytechnic Institute

PLANT BIOTECHNOLOGY CONTRIBUTED PAPERS II

Moderator: John J. Finer, The Ohio State University

5:00 pm – 5:45 pm Plant Contributed Paper Session Evergreen ABC

5:00	P-1014	Tubulin Manipulations Alter Wood Properties and Drought Tolerance Characteristics in Populus *Rashant Swamy*, University of Georgia, Shawn Mansfield, Jeng-Der Chung, Christopher Frost, Scott Harding, and C.-J. Tsai
5:15	P-1016	Tissue Culture and Induced Mutation of Giant Miscanthus Dinum Perera,Mississippi State University, Brian S. Baldwin, and Nancy A. Reichert
5:30	P-1017	Overexpressing CBP and AVP1 Confers Drought Tolerance in Rice (Oryza sativa) S. Y. Lee, North Carolina State University, R. Gaxiola, G. Yang, D. Robertson, and R. Qu

Daily Program	Abstracts & Posters
Keynote Speaker	Keynote Symposium	Plant Contributed Papers
Sunday, June 3	International Conference	Animal Posters
Monday, June 4	Animal Symposia & Workshops	Education Posters
Tuesday, June 5	Plant Symposia & Workshops	Plant Posters
Wednesday, June 6	Animal Contributed Papers	Addendum Booklet
Thursday, June 7