Tuesday, June 9

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Daily Program
Abstracts & Posters
Saturday, June 6 Keynote Symposium Plant Contributed Papers
Sunday, June 7 Plenary Symposia Animal Posters
Monday, June 8 Animal Symposia & Workshops Plant Posters
Tuesday, June 9 Joint Symposia Education Posters
Wednesday, June 10 Plant Symposia & Workshops Addendum Booklet
    Education Symposia  

TUESDAY, JUNE 9

7:00 am – 5:00 pm Registration Foyer

NATIONAL AND INTERNATIONAL REGULATORY ASPECTS OF GEOS

Conveners:       John M. Cordts, USDA/APHIS, and Thomas J. Flynn, US FDA

8:00 am – 10:00 am Plenary Symposium Ballroom A

Numerous genetically engineered organisms, both plant and animal, have been developed over the last 30+ years for use in widely variable applications. While many countries and regions around the world have embraced the use of genetically engineered organisms, others continue to wrestle with issues of food safety, environmental risk, ownership and patenting of seed resources, and a variety of socio-economic changes. Speakers in this session will explore on-going trends in regulatory agencies and potential implications for international research and trade.

8:00   Introduction (J. M. Cordts and T. J. Flynn)
8:05 PS-10 Interaction of Global Regulatory Approaches and Testing Methodology with Trade
Raymond D. Shillito, Bayer CropScience LP
8:40 PS-11 Safety Assessment of Plant-incorporated Protectants (PIPs) by the United States Environmental Protection Agency
John L. Kough, US-EPA
9:15 PS-12 Animal Clones and their Progeny, FDA’s GE Animal Guidance, and Other Recent Actions
Jeffrey Jones, FDA
9:50   Discussion

 

10:00 am – 10:30 am Coffee Break Ballroom B&C

 

IMPACT OF CELL CONTAMINATION ON RESEARCH

Conveners:       John W. Harbell, Mary Kay, Inc., and Tohru Masui, National Institute of Biomedical Innovation, Japan

10:30 am – 12:30 pm Animal Symposium Meeting Room 1

In 2007, a group of leading cell culture scientists petitioned the United States Secretary of Health and Human Services to develop an active program for cell line authentication. They cited the importance of cell cultures as research and teaching tools in diverse fields such as medicine, genetics, drug discovery, reconstructive medicine, vaccine development and basic research. Despite the importance of these tools, there exists a gap in the fundamental principles of cell line quality control across too much of the user community. In particular, they pointed to the documented problems with cell line authentication. The frequency that research has been compromised by the use of contaminated or misidentified cell lines has become a clear concern to scientists, granting agencies and scientific journals. This is not a new issue for in vitro research or the SIVB. Over three decades ago, Dr. Nelson-Rees documented the contamination of many cell lines with the rapidly growing HeLa line. Since then, other individuals and organization have worked to identify problem cell lines and develop methods for authentication. This symposium was organized to bring speakers from three of the major cell repositories in Asia, Europe, and North America. Their presentations will address the extent of the problem in each area, their technical approaches for authentication, and overall programs to reduce the use of misidentified cell line. While this symposium will focus largely on animal-derived cells lines, the principles would be applicable to all cell types.

10:30   Introduction (J. W. Harbell and T. Masui)
10:35 A-15 Online Verification of Cell Line Authenticity Using an International Reference Database
Wilhelm G. Dirks, DSMZ
11:10 A-16 Misidentification of Animal Cell Lines: Impact on Research
Yvonne A. Reid, ATCC
11:45 A-17 Simple Sequence Length Polymorphism (SSLP) Analysis to Determine the Strain from Which Mouse Cell Lines are Derived
Yukio Nakamura, RIKEN BioResource Center
12:20   Discussion

 

INTELLIGENT MEDIA DESIGN

Conveners:       Jean L. Roberts, Dow Agrosciences, LLC, and Soverin Karmiol, SAFC BioSciences

10:30 am – 12:30 pm Joint Symposium Ballroom C1&C2

Culture media and the physical environment cannot be overlooked as an influence in tissue culture experiments. The utilization of nutrients in media by the cells over the culture cycle causes physicochemical changes (pH, osmolarity) in the medium; nutrient depletion can induce different metabolic pathways in the cells during different culture phases, and the accumulation of toxins and free radicals can induce cell death. We will describe these changes and demonstrate how media optimization addresses these potential sources of stress in cultured cells. The session includes presentations from the plant and animal tissue culture communities.

10:30   Introduction (J. L. Roberts and S. Karmiol)
10:35 J-4 Design, Optimization and Handling of Mammalian Cell Culture Media
Paul J. Price, D-Finitive Cell Technologies
11:00 J-5 A Systematic Approach to Media Development with an Emphasis on Optimization at the Basal Component Level
Soverin Karmiol, SAFC Biosciences
11:25 J-6 Medium Changes During the Culture Cycle Influence Tobacco Suspension Cell Physiology
Jean L. Roberts, Dow AgroSciences
11:50 J-7 Investigation of the Metabolism of Nicotiana tabacum Cells Using the Respiratory Monitoring System (RAMOS)David A. Ullisch, RWTH Aachen University
12:15   Discussion

 

PRODUCTION OF VALUE ADDED PRODUCTS IN TRANGENIC PLANTS

Convener:         Maria N. Somleva, Metabolix, Inc.

10:30 am – 12:30 pm Plant Symposium Ballroom A

As renewable sources of energy and raw materials, agricultural feedstocks are the ultimate replacement of declining oil reserves. Plants use solar energy and carbon dioxide to make thousands of complex and functional biochemicals beyond the capability of the modern synthetic chemistry. Plant-based products include fine and bulk chemicals, pharmaceuticals, polymers, resins, food additives, bio-colorants, adhesives, solvents, and lubricants. Plant inputs are already cost-competitive for some of these products and have great social and environmental benefits. Genetic engineering of crop plants as production platforms for modified and novel materials provides a sustainable solution for high value eco-friendly industrial applications. This approach requires new science and technology to be directed at better understanding and modifying plant development and metabolism, designing new harvesting and extraction technologies, and developing improved manufacturing systems. The speakers in this session will highlight recent advances in engineering bioenergy and industrial crops for production of biodegradable plastics, rubber, and modified plant oils.

10:30   Introduction (M. Somleva)
10:35 P-23 Oilseeds as Factories for Renewable Fuels and Materials
Jan Jaworski, Donald Danforth Plant Science Center
11:00 P-24 Manipulation of Reserve Content in Cottonseeds to Influence Oil, Protein, and Fiber Content
Kent D. Chapman, University of North Texas
11:25 P-25 Engineering Rubber Production in Plants
Maureen C. Whalen, ARS/USDA
11:50 P-26 Biomass and Industrial Oilseed Crops as Platforms for Production of Biodegradable Plastics
Maria N. Somleva, Metabolix, Inc.
12:15   Discussion

 

CELLULAR PATHOLOGY AND CELL LINE ESTABLISHMENT & CHARACTERIZATION

Moderator:        Raziel S. Hakim, Howard University

1:30 pm – 2:30 pm Animal Interactive Poster Session Ballroom B&C
   A-2014 In Vitro Determination of Protective Antigen Stability in a Novel, Transdermal Anthrax Vaccine
Kevin E. Knockenhauer, State University of New York – Stony Brook, Katarzyna M. Sawicka, Elizabeth J. Roemer, and Sanford R. Simon
   A-2015  Temperature-induced Morphological Changes and Microsporidia Growth in the Ep-1 Cell Line
S. Richelle Monaghan, University of Waterloo, N. C. Bols, and L. E. J. Lee
   A-2016  Establishment of a Monarch Butterfly (Danaus plexippus, Lepidoptera: Danaidae) Cell Line and Its Susceptibility to Insect Viruses
James J. Grasela, USDA/ARS, and Arthur H. McIntosh
   A-2017 Immunocytochemistry and Image Analysis of Beta-catenin Redistribution in Normal Human Colon Cell Cultures Treated with Disinfection By-products
Ernest Winkfield, US EPA, Mary Moyer, and Anthony DeAngelo
   A-2018  OLGA: A Case of Mistaken ID
Lucy E. J. Lee, Wilfrid Laurier University, William J. Martin, Andre W. Christie, Marc Frischer, Thomas Soin, Guy Smagghe, Heather Braid, and Robert Hanner
   A-2019  Development of an Aiptasia pallida Cell-culture System to Study Cnidarian-Dinoflagellate Symbiosis
Jan Denofrio, Stanford University, and John Pringle
   A-2020  Development of a Liver Cell Line from Fathead Minnow, Pimephales promelas, and Their Molecular and Biochemical Characterization
Lucy E. J. Lee, Wilfrid Laurier University, N. Vo, J. Werner, R. Weil, N. D. Denslow, and R. D Law

 

 

CELLULAR MODELS – EXTRACELLULAR MATRIX, 3D MODELS, AND CRYOPRESERVATION

Moderator:        Lia H. Campbell, Cell & Tissue Systems, Inc.

1:30 pm – 2:30 pm Animal Interactive Poster Session Ballroom B&C
  A-2021 Comparative Gene Expression Analysis of 1- and 2-Methylanthracene in Rat Liver Stem Cells
Joon-Suk Park, Michigan State University, P. Babica, J. E. Trosko, and B. L. Upham
  A-2022 Collagen Production by Normal Human Dermal Fibroblast Cells in Serum-free Media
Nicole Napolitano, State University of New York – Stony Brook, M. Monaghan, E. Roemer, and S. Simon
  A-2023 Analysis of Extracellular Matrix Synthesized by Human Bronchial Smooth Muscle Cells In Vitro
Sumaria Zamurrad, State University of New York – Stony Brook, L. J. Crawford, S. R. Simon, and E. J. Roemer
  A-2024 The Use of Collagen Matrices for Control of MMP and Serine Proteinase Activity in Chronic Wounds
Katelyn D. Fenwick, State University of New York – Stony Brook, S. R. Simon, F. Daccueil, and E. J. Roemer
  A-2025 Validating MTS as an Alternative Viability Assay to MTT on the Human 3-D Tissue Models, EpiAirwayTMand EpiDermTM
Catherine R. Kavanagh, State University of New York – Stony Brook, L. J. Crawford, S. R. Simon, and E. J. Roemer
  A-2026 Limited Success in the Long-term Culture and Maintenance of Function of Non-plateable Lots of Cryopreserved Primary Human Hepatocytes on a 2-dimensional Surface
Thomas J. Flynn, US FDA, and M. C. Garcia
  A-2027 Evaluation of Apoptosis During Static Storage of Pancreatic Islet CellsL
ia
H. Campbell, Cell & Tissue Systems, Inc., A. Vazquez, Z. Chen, and K. G. M. Brockbank

 

GENETIC TRANSFORMATION FOR CROP IMPROVEMENT

Moderator:        Michael Spencer, Monsanto Company

1:30 pm – 2:30 pm Plant Interactive Poster Session Ballroom B&C
  P-2010 Hypoallergenicity of Transgenic Peanut (Arachis hypogea L.) Is Transmitted to the Progeny
Anthony Ananga, Alabama A&M University, Koffi Konan, and Hortense Dodo
  P-2011 Reducing Lignin Content in Bahaigrass (Paspalum notatum Flugge) by RNAi Suppression of 4-Coumarate-CoA Ligase
Walid M. Fouad, University of Florida-IFAS, L. Martin, W. Vermerris, and F. Altpeter
  P-2012 Expression Analysis and Functional Characterization of Rice Genes Encoding microRNA156s and Their Application in Plant Genetic Improvement
Dayong Li, Clemson University, Xue Liu, Qian Hu, Lihuang Zhu, and Hong Luo
  P-2013 Biotechnology for Castor Oil Production
Grace Chen, USDA/ARS
  P-2014 Heterologous Expression of Arabidopsis H+-PPase Enhances Salt and Drought Tolerance in Transgenic Creeping Bentgrass (Agrostis stolonifera L.)
Zhigang Li,Clemson University, Christian M. Baldwin, Qian Hu, Haibo Liu, and Hong Luo

 Tuesday, June 9
Odd Poster Authors will be present
2:30 pm – 3:30 pm

 

 

EVALUATION OF HUMAN HEALTH ENHANCING PRODUCTS THROUGH ANIMAL ASSAYS

 Conveners:       Nirmal Joshee, Fort Valley State University and Elizabeth J. Roemer, State University of New York – Stony Brook

3:30 pm – 5:00 pm Joint Symposium Ballroom A

Botanical materials have long been a fertile source of bioactive components for human health care. There are at least 250,000 species of plants out of which more than one thousand have been found to possess significant anticancer properties. Botanical extracts are frequently used in the cosmeceutical industry, and are finding expanding applications as anti-inflammatory and wound healing pharmaceuticals. This session will review recent advances in understanding the mechanisms of action and structure-function relationships of botanically derived agents at the molecular, cellular and physiological levels.

3:30   Introduction (N. Joshee and E. J. Roemer)
3:35 J-8 Use of Multiple In Vitro Assays to Study Pleiotropic Anti-Inflammatory and Anti-metastatic Actions of Low Molecular Weight Natural Products of Botanical Origin
Sanford R. Simon, State University of New York – Stony Brook
4:00 J-9 Anti-tumor Mechanisms of Various Scutellaria Extracts and Constituent Flavonoids
Prahlad Parajuli, Wayne State University and Karmanos Cancer Institute
4:25 J-10 Plant Extracts for Cosmeceutical Applications: Genetic Profiling of Cell Responses
Melissa Monaghan, State University of New York – Stony Brook
4:50   Discussion

 

INSECT PATHOGEN FIELD EXPLORATION AND IN VITRO PROPAGATION

Conveners:       Guido F. Caputo, National Resources Canada, and Pauline O. Lawrence, University of Florida – Gainesville

3:30 pm – 5:00 pm Animal Roundtable Meeting Room 3

Insects, the most diverse, damaging, and adaptable of all species can be found in the most critical of environments ranging from ocean bottoms to swamps, desserts and forests. Viruses, fungi, bacteria, and protozoa, can kill insects and are often incorporated into an integrated pest management control program.  These pathogenic microorganisms are naturally occurring in nature and can be reproduced to working volumes in laboratories. This session will focus on the latest molecular identification techniques and genetic engineering protocols and procedures for locating, collecting and processing these organisms.  Discussion will include susceptible hosts for their in vitro propagation and the regulatory agencies and their requirements before they can be re-introduced back to the field as biological control agents.

3:30   Introduction (G. F. Caputo and P. O. Lawrence)
3:35 A-18 Current Trends on the Identification and Propagation of Entomopathogenic Nematodes
S. Patricia Stock, University of Arizona
3:40 A-19 In Vitro Culture of an Entomopoxvirus from a Parasitic Wasp and its Potential to Control Tephritid Fruit Flies
Pauline O. Lawrence, University of Florida
 3:45 A-20 Foreign Exploration of Potential Entomopathogen Agents for the Control of the Diamondback Moth, Plutella xylostella:a Case Study
James Grasela, USDA/ARS/BCIRL
3:50 A-21 Baculoviruses as Effective Bio-control Agents of Forest Pests
Guido F. Caputo, Natural Resources Canada
3:55   Discussion

 

PROTOCOLS FOR CELL CHARACTERIZATION, IDENTIFICATION, AND CONTAMINATION TESTING

 Convener:         Amy M. Wright, CIBA Vision Corporation

3:30 pm – 5:00 pm Animal Symposium Meeting Room 1

Data collected from in vitro tissue culture studies are used in the determination and evaluation of novel products and cell responses in the drug, medical device and biomedical industries.  To insure that the data collected are accurate, the scientist needs tools for cell culture identification, possible background contamination, and cross culture contamination.  Increased scrutiny within the publishing establishment has begun to require that the studies performed with cell culture systems are representative as such. This symposium will cover areas of concern; Endotoxin or bacterial contamination, cell culture identification, protein levels of cell cultures or within assays for biomarker determination.

3:30   Introduction (A. M. Wright)
3:35 A-22 Using the PTS (Portable Test System): Hand-held Technology for Rapid Assessment of Microbial Contamination
Norman R. Wainwright, Charles River Laboratories
4:00 A-23 Cell Line ID and Contamination Detection by Allele-specific Real-time TaqMan PCR Assays
Caifu Chen, Life Technologies
4:25 A-24 Mycoplasmal Contamination:  Risk Reduction Strategies and Diagnostic Methods
Jill Mariano, Bionique Testing Laboratories
4:50   Discussion

 

RISK ASSESSMENT AND TRANSGENE CONTAINMENT IN TRANSGENIC CROPS

 Convener:         C. Neal Stewart, Jr., University of Tennessee

3:30 pm – 5:00 pm Plant Symposium Ballroom C1&C2

To the surprise of many scientists in plant biotechnology, transgene flow from crops to non-transgenic crops and wild relatives is still of deep regulatory, environmental and consumer concern. This symposium will critically examine the risks that might result from transgene flow in the forms of hybridization and introgression. Case studies will be presented. Among the more prominent cases are those involving creeping bentgrass and alfalfa, in which gene flow has been an important determinant in preventing commercialization, as well as post-commercialization of introgression of transgenes from herbicide-tolerant canola. Therefore, it is clear that biocontainment technologies would be valuable assets toward reducing transgene flow risks and inadvertent admixture of transgenes in erstwhile nontransgenic crops. Fortunately, there are recent important developments of containment biotechnologies for removing transgenes, inducing male sterility, and precision tools for altering gene transmission in plants. The symposium will look back at past situations, examine current questions, and look forward to future containment of transgenes. The latter will be especially important as we consider future applications of biotechnology in the development of perennial plants for the new bioeconomy.

3:30   Introduction (C. N. Stewart)
3:35 P-27 Gene Flow in a Model GM Crop: Canola
Suzanne I. Warwick, Agriculture and Agri-food Canada
4:00 P-28 Strategies for Gene Confinement in Genetically Modified Perennial Plants Used for Biofuels
Albert P. Kausch, University of Rhode Island
4:25 P-29 Seed Targeted Gene Confinement Strategies
Melvin J. Oliver, University of Missouri
4:50   Discussion
5:00 pm – 5:30 pm SIVB Business Meeting
(All Members Are Urged to Attend)Student Award Presentations
Meeting Room 3
5:45 pm – 10:00 pm An Evening at the Magnolia Plantation
Admittance by Advance Ticket Holders Only
Magnolia Plantation