Sunday, June 7

2009logo

Daily Program
Abstracts & Posters
Saturday, June 6 Keynote Symposium Plant Contributed Papers
Sunday, June 7 Plenary Symposia Animal Posters
Monday, June 8 Animal Symposia & Workshops Plant Posters
Tuesday, June 9 Joint Symposia Education Posters
Wednesday, June 10 Plant Symposia & Workshops Addendum Booklet
  Education Symposia

SUNDAY, JUNE 7

7:00 am – 5:30 pm Registration Foyer

GENE TARGETING

Conveners:       Richard Heller, Old Dominion University, and Tzvi Tzfira, University of Michigan

8:00 am – 10:00 am Plenary Symposium Ballroom A

Gene targeting is one of the most sought-after technologies for animal and plant improvement. Gene targeting can potentially be achieved by homologous recombination or by non-homologous end joining, leading to a variety of outcomes, ranging from gene replacement and gene repair to site-specific integration of foreign DNA molecules and site-specific mutagenesis. Speakers in this session will introduce the audience to the biological barriers and to the biotechnological solutions for achieving genome editing and gene transfer in higher eukaryotic cells.

8:00 Introduction (R. Heller and T. Tzfira)
8:05 PS-1 Cytoplasmic and Nuclear Trafficking of Plasmids During Gene Delivery
David Dean, University of Rochester
8:30 PS-2 Gene Delivery
Richard Heller, Old Dominion University
8:55 PS-3 Motivating Gene Targeting: How an Understanding of the Basic Mechanisms and Regulation of Recombination May Yet Lead to Efficient Gene Replacement in Higher Plants
Anne Britt, University of Califorina-Davis
9:20 PS-4 Zinc Finger Nuclease-mediated Gene Targeting
Joseph F. Petolino, Dow AgroSciences
9:45 Discussion
10:00 am – 10:30 am Coffee Break Ballroom B&C

BIO/TISSUE ENGINEERING

Conveners:       Elizabeth J. Roemer, State University of New York – Stony Brook, and Paul J. Price, D-Finitive Cell Technologies

10:30 am – 12:00 pm Animal Symposium Meeting Room 1

Tissue Engineering combines cells, matrices, support structures or scaffolds and a suitable environment to produce proto-tissues for the understanding of Biological Development, Toxicity Testing and Regenerative Medicine.  The use of support systems and stem or tissue cells offer unique opportunities for the repair, enhancement or replacement of damaged or diseased tissues and organs.  This Symposium will look at the formation of proto-tissues through the interdisciplinary efforts of engineering, cellular maturation and the understanding of biological systems.

10:30 A-3 Introduction to Regenerative Medicine
Paul J. Price, D-Finitive Cell Technologies
10:45 A-1 Tissue Engineering and Ex Vivo Tissue Test Systems
Karen J. L. Burg, Clemson University
11:20 A-2 Injectable Hydrogels for Brain Tissue Regeneration After Traumatic Brain Injury (TBI)Ning Zhang, Clemson University and MUSCc
11:50 Discussion

STUDENT ORAL PRESENTATION COMPETITION OF PLANT BIOTECHNOLOGY RESEARCH

Moderator:        Fredy Altpeter, University of Florida-IFAS

Panel of Plant Biotechnology Experts Evaluating the Contestants: Kan Wang, Iowa State University; Vincent Wingate, Biolex Therapeutics; and John Finer, Ohio State University

10:30 am – 12:30 pm Plant Contributed Paper Competition Ballroom A

To support the Society’s vision to encourage education and scientific informational exchange and recognize outstanding students, the Plant Biotechnology section of SIVB offers an Oral Presentation Competition of Plant Biotechnology Research. Finalists were selected based on the quality of the abstracts. A panel of judges will evaluate the presentations. Criteria are experimental design, data analysis, proper interpretation of the results, originality of the study, technical difficulty, appearance of the presentation and ability of the student to respond to questions. Winners will be presented with a certificate and a cash award at the Plant Biotechnology Section’s Business Meeting.

10:30 P-1000 Expressing the Gibberllin Catabolizing Enzyme AT-GA-ox1 in a Low-input Turfgrass (Paspalum notatum Flugge) Improves Turf Quality and Field Performance
Paula Lomba, University of Florida-IFAS, K. Kenworthy, and F. Altpeter
10:54 P-1001 Genetic Engineering of Turfgrass and Rice with Two Novel Antimicrobial Peptides for Enhanced Disease Resistance
Man Zhou, Clemson University, Allision Cason, and Hong Luo
 11:18  P-1003 Highly Efficient Suppressor-dependent Protein Expression in Plants with a Foxtail Mosaic Virus Vector
Zun Liu, Baylor University, Christopher M. Kearney
11:42  P-1004 Regeneration of Arachis paraguariensis Through Different Morphogenic Pathways
Olubunmi O. Aina, University of Florida, K. H. Quesensberry, and M. Gallo
 12:06  P-1005 Exogenous Tocopherol and Ascorbic Acid Improve In Vitro Recovery of Cryopreserved Rubus Shoot Tips
Esther E. Uchendu, Oregon State University, and Barbara M. Reed

POST DOCTORAL ORAL PRESENTATION COMPETITION OF PLANT BIOTECHNOLOGY RESEARCH

Moderator:        Michael Bosela, Indiana University – Purdue University at Fort Wayne

Panel of Plant Biotechnology Experts Evaluating the Contestants: Paula Pijut, USDA Forest Service; Vibha Srivastava, University of Arkansas; and Randall P. Niedz, USDA-ARS

10:30 am – 12:30 pm Plant Contributed Paper Competition Ballroom C1&C2

To support the Society’s vision to encourage education and scientific informational exchange and recognize outstanding post docs, the Plant Biotechnology section of SIVB offers an Oral Presentation Competition of Plant Biotechnology Research. Finalists were selected based on the quality of the abstracts. A panel of judges will evaluate the presentations. Criteria are experimental design, data analysis, proper interpretation of the results, originality of the study, technical difficulty, appearance of the presentation and ability of the student to respond to questions. Winners will be presented with a certificate and a cash award at the Plant Biotechnology Section’s Business Meeting.

10:30 P-1006 Host-delivered RNAi: An Effective Strategy to Silence Nematode Genes in Transgenic Hairy Roots of Soybean
Jiarui, Li, Kansas State University, Timothy C. Todd, William T. Schapaugh, and Harold N. Trick
10:50 P-1007 Higher Accumulation of F1-V Fusion Recombinant Protein in Plants After Induction of Protein Body Formation
Maria Lucrecia Alvarez, Arizona State University, Emel Topal, Federico Martin, and Guy A. Cardineau
11:10 P-1008 Transposon Mutagenesis of Soybean (Glycine max) Using the Rice MITE mPing
Charles N. Hancock, University of Georgia, F. Zhang, D. M. Tucker, S. R. Wessler, and W. A. Parrott
11:30 P-1009 TILLING for Peanut Improvement
Joseph E. Knoll, University of Georgia, M. L. Ramos, and P. Ozias-Akins
11:50 P-1010 Integration and Expression of E. coli L-aspartate-alpha-decarboxylase in Tobacco Chloroplasts Enhances Photosynthesis and Biomass Accumulation Following High Temperature Stress
Walid M. Fouad, University of Florida-IFAS, and Fredy Altpeter  
12:10 P-1011 Using Endogenous Vitis Genes to Produce Disease Resistant Transgenic Grapevines
Sadanand A. Dhekney, University of Florida-IFAS, Z. T. Li, T. W. Zimmerman, and D. J. Gray

CELL CULTURE OF MARINE INVERTEBRATES

Conveners:       James J. Grasela, USDA ARS BCIRL; Lucy E. J. Lee, Wilfrid Laurier University; and Guy Smagghe, Ghent University

1:30 pm – 3:00 pm Animal Symposium  Meeting Room 1

For a number of years marine invertebrate cell culture research has lagged behind other areas of invertebrate cell culture, particularly insects, in the development of established cell culture lines.Indeed, tremendous effort has been made to establish long-term (>1 month) continuous invertebrate cell lines,although to date there are no published reports of a successfully established marine invertebrate cell culture.   Nevertheless, many primary marine invertebrate cell cultures have been developed for the purpose of providing novel biologically active chemical compounds with pharmaceutical properties. Furthermore, the study of diseases from many valuable commercial species such as crayfish, lobsters, shrimps, mussels, oysters, and clams has also been an incentive to develop viable marine cell cultures. This session will focus on the continueddevelopment of marine invertebrate cell culture, the potential obstacles encountered in an attempt to establish long-term marine cell cultures, and some of the new technologies available that may help to overcome these problems.

1:30 Introduction (L. E. J. Lee)
1:35 A-4 Transfection of Marine Sponge Cells to Produce a Cell Line
Shirley A. Pomponi, Harbor Branch Oceanographic Institute
2:00 A-5 Stem Cells from Marine Invertebrates – Perspectives and Prospective
Baruch Rinkevich, National Institute of Oceanography
2:25 A-6 Crustacean and Their Pathogens: Developing Susceptible Host Cell Lines and Alternative Approaches
Karen G. Burnett, Grice Marine Laboratory
2:50 Discussion

CRYOPRESERVATION

Conveners:       David D. Ellis, USDA/ARS, and Lia H. Campbell, Cell and Tissue Systems, Inc.

1:30 pm – 3:00 pm Joint Symposium   Ballroom C1&C2

The cryopreservation of living cells is becoming routine with many cell, organ or tissue types in both plants and animals. Despite this, many cellular aspects of cellular, biochemical, structural and molecular biology with respect to the ability to survive cryopreservation are unknown even though many of these aspects are likely to be common between animal and plant cells. We know that intracellular ice formation and growth is lethal in most systems and that dehydration or removal of the water from the cells and intercelluar spaces is often required. Yet cells require this aqueous environment to survive. This symposium will explore current state of the art in plant and animal cryobiology with an emphasis on sustaining a viable, living cell capable of further growth and manipulation when removed from liquid nitrogen.

1:30 Introduction (D. D. Ellis and L. H. Campbell)
1:35 J-1 Storage and Distribution Issues for Cryopreserved Cells and Tissues
Kelvin G. M. Brockbank, Cell & Tissue Systems, Inc.
2:00 J-2 Preservation of Biomaterials in the Dry State: Lessons from Nature
John H. Crowe, University of California-Davis
2:25 J-3 Beyond the Cell Wall: Comparisons Between Plant and Animal Cell Cryopreservation
Hugh Pritchard, UK Seed Conservation
2:50 Discussion

TRANSGENIC APPROACHES IN OIL MODIFICATION

Convener:         T. E. Clemente, University of Nebraska

1:30 pm – 3:00 pm Plant Symposium   Ballroom A

A significant amount of progress has been made in understanding fatty acid biosynthesis in plant cells, in addition to a wealth of DNA sequence information that is available from a plethora of organisms. These biochemical and genomics resources will serve as valuable tools that will enable the translation of this information to applied technologies. The plant kingdom possesses a large repository of fatty acids with different structures and physical properties that hold great potential as sources for food, biofuel and biomaterial applications. However, many of these novel fatty acids accumulate in plants that lack agronomic potential. Hence, the ability to manipulate oil metabolism in scalable feedstocks through genetic transformation of established lipid feedstocks offers a realistic avenue for the synthesis of novel fats at production levels that can meet the demands of the market. The speakers in this session will describe strategies for the modification of seed oils for various market applications. A round-table discussion will be held at the end of the session allowing for an open dialogue on the promise and hurdles facing the development and marketing of modified feedstocks for the production of novel oils for food, feed, and biomaterials.

1:30 Introduction (T. E. Clemente)
1:35 P-1 Modifying Soybean Oil for Feed and Fuel
T. E. Clemente, University of Nebraska-Lincoln
2:00 P-2 Enhancing Soybean Seed Lipid Biosynthesis for Nutritional, Energy, and Industrial Applications
Anthony J. Kinney, DuPont Experimental Station
2:25 P-3 Camelina sativa, a Potential Oilseed Platform for the Production of High-value Industrial Oils
Edgar B. Cahoon, University of Nebraska-Lincoln
2:50 Discussion

2009 IN VITRO BIOLOGY OPENING CEREMONY

 Program Chair:              David D. Songstad, Monsanto Company

3:00 pm – 5:30 pm Opening Ceremony   Ballroom A

Conveners:       Todd J. Jones, BASF, David D. Songstad, Monsanto Company

3:00 Welcome and Opening Remarks:Todd J. Jones, President, Society for In Vitro Biology
3:05 ROBERT H. LAWRENCE, JR. KEYNOTE SYMPOSIUMIntroduction (T. J. Jones and D. D. Songstad)
KS-1 Introduction to the DOE BioEnergy Science Center
Russ Miller,Manager of Technology Transfer and Partnerships
4:00 Questions and Answers
4:15 2009 SOCIETY FOR IN VITRO BIOLOGY AWARDS CEREMONY
4:20 2009 Young Scientist Award Recipient (Award to be presented at Section Meeting)Tzvi Tzfira, University of Michigan

2009 Fellow Award Recipients (Awards to be presented at Section Meetings)
Daniel C. W. Brown, Agriculture & Agri-Food Canada
Lia H. Campbell, Cell & Tissue Systems, Inc.
Michael E. Kane, University of Florida
Pamela J. Weathers, Worcester Polytechnic Institute

4:25 2009 Distinguished Service Award Presentations (Awards to be presented by Todd J. Jones):John W. Harbell, Mary Kay Inc.
Peggy J. Ozias-Akins, University of Georgia
Wayne A. Parrott, University of Georgia
T. Michael Spencer, Monsanto Company
Kan Wang, Iowa State University
2009 Lifetime Achievement Award Presentations:
4:30 (Introduction by Lucy E. J. Lee; Acknowledgement by Dr. Bayne to follow)Christopher J. Bayne, Oregon State University
4:45 (Introduction by Gregory C. Phillips; Acknowledgement by Dr. Collins to follow)
Glenn B. Collins, University of Kentucky
5:00 (Introduction by Chieri Kubota; Acknowledgement by Prof. Kozai to follow)
Toyoki Kozai, Chiba University
5:15 (Introduction by Sandra L. Schneider and Tetsuji Okamoto; Acknowledgement by Prof. Namba to follow)
Masayoshi Namba, Okayama University Medical School
5:30 pm – 6:30 pm 2009 IN VITRO BIOLOGY
OPENING CEREMONY RECEPTION
  Ballroom B&C
5:30 pm – 7:00 pm IVACS Student Poster Competition   Ballroom B&C
5:30 pm – 7:30 pm Exhibits and Posters   Ballroom B&C

 Sunday, June 7
Odd Poster Authors will be present
6:30 pm – 7:30 pm

 

STUDENT NETWORKING SYMPOSIUM: BUILD YOUR WRITTEN/ORAL SKILLS, ADVERTISE YOURSELF, AND FIND THAT PERFECT JOB

Convener:         Phillip J. Kauth, University of Florida

7:30 pm – 9:30 pm Education Symposium   Meeting Room 3

Professional networking is easily the most effective and powerful strategy for conducting a job search in any economy. In 2009, with the state of the current economy and the dismal employment outlook, it may be the only way to identify and locate available positions and help get a foot in the door. The primary responsibilities of the job seeker in this process are to be educated about their prospective audience, the current trends in their targeted field, and to make sure he/she is equipped with the best possible tools and have them readily available. After conducting some basic research on where the job pools may exist for graduates with a background in biology and research with this particular focus, there appears to be reason for optimism. The areas of health care research, pharmaceuticals, biotechnology and genetically driven research are not occupational areas included in the current negative employment projections. There is every reason to believe that a well prepared candidate, with an effective job search plan, will be successful in securing a position in their chosen field.

7:30 Introduction (P. J. Kauth)
7:35 E-1 Build Your Written/Oral Skills, Advertise Yourself and Find That Perfect Job
Karen Eippert, College of Charleston
8:00 Workshop