Monday, June 8

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Daily Program
Abstracts & Posters
Saturday, June 6 Keynote Symposium Plant Contributed Papers
Sunday, June 7 Plenary Symposia Animal Posters
Monday, June 8 Animal Symposia & Workshops Plant Posters
Tuesday, June 9 Joint Symposia Education Posters
Wednesday, June 10 Plant Symposia & Workshops Addendum Booklet
  Education Symposia

MONDAY, JUNE 8

7:00 am – 6:00 pm Registration Foyer

INTEGRATION OF GENOMICS, PROTEOMICS AND METABOLOMICS

Conveners:       Sixue Chen, University of Florida, and Jacqueline A. Jordan, Clayton State University

8:00 am – 10:00 am Plenary Symposium Ballroom A

Genomics, Proteomics, and Metabolomics are the new pillars of modern biological sciences. Systems Biology integrates these three “omics” to study and understand the entire living organism. Conceptual and technical advancements in systems biology have created numerous opportunities in research and this contributes to the understanding of the complexities within biological systems. As these technologies advance to common use, it is essential for biologists to develop strategies to handle, analyze and integrate large quantities of data. Speakers in this session will introduce recent technological developments in different “omics” areas. Real applications in data integration and elucidation of biological processes and networks will be introduced. In this session, future directions will be highlighted and key issues facing the area of systems biology discussed.

8:00 Introduction (S. Chen and J. A. Jordan)
8:05 PS-5 Introduction to Research in Systems Biology
Jacqueline A. Jordan, Clayton State University
8:25 PS-6 Beyond Gene Clustering of Microarray Data
W. Jim Zheng, Medical University of South Carolina
8:45 PS-7 Integrating High-throughput and Genomics Technologies in Least Expected Places
Maja Kodani, Centers for Disease Control and Prevention
9:05 PS-8 Metabolomics and Integrated Functional Genomics Reveal Novel Information Related to Medicago Secondary Metabolism
Lloyd Sumner, Noble Foundation
9:25 PS-9 Towards a Systems Analysis of Plant Molecular Networks
Sixue Chen, University of Florida
9:45 Discussion
10:00 am – 10:30 am Coffee Break Ballroom B&C

 

PLANT IN VITRO CULTURE LAB EXERCISES

Convener:         Michael E. Kane, University of Florida

10:30 am – 12:00 pm Plant Workshop Ballroom C1&C2

College science teachers often need new and exciting approaches to engage students during laboratory sessions. In vitro biology exercises are easy to incorporate into science courses. Many students have limited exposure to in vitro biology, yet become very interested once exposed to various in vitro techniques. Including in vitro techniques into laboratory curriculum is an exciting approach to teach students critical thinking and basic laboratory skills. However, the availability of reliable, effective, and efficient laboratory exercises is often limited. During this symposium an overview of four unique in vitro plant tissue culture laboratory exercises will be presented.

10:30 Introduction (M. E. Kane)
10:40 P-4 Using Tobacco to Teach Plant Tissue Culture and Transformation
Margaret Young, Elizabeth City State University
11:00 P-5 A Classroom Exercise in Asymbiotic Orchid Seed Germination: The Whole Story
Timothy R. Johnson and Philip Kauth, University of Florida
11:20 P-6 Using Plant Tissue Cultures to Demonstrate Mineral Nutrient Deficiencies: Comparison with Conventional Hydroponic Techniques
Michael Bosela, Indiana University- Purdue University at Fort Wayne
11:40 P-7 Synthetic Seed Technology Demonstrated Through a Novel Teaching Exercise
Michael E. Kane, University of Florida

JUNE BRADLAW MEMORIAL SYMPOSIUM: TOXICOLOGY IN THE 21ST CENTURY

Conveners:       Eugene Elmore, University of California – Irvine, and Brad L. Upham, Michigan State University

10:30 am – 12:30 pm Animal Symposium Meeting Room 1

The current animal model systems for predicting both toxicology and drug efficacy for human responses have been very ineffective. This was evident from the studies with cancer therapeutics where adverse events relating to critical tissues were not adequately predicted by animal studies. The National Research Council published a report in 2007 (http://dels.nas.edu/dels/rpt_briefs/Toxicity_Testing_final.pdf) that established the vision and strategy to implement a paradigm shift from classic animal models for predicting human toxicity and risk to a focus on in vitro model systems that use human cell systems derived from different tissues to identify toxicity pathways that would be more predictive of human responses. The vision suggests that by using the best current science, we would ultimately be able to identify toxic agent mechanisms that would minimize or eliminate testing in animal models.   Full implementation of the vision and strategy will require substantial funding, coordinated efforts, and application of our best scientific approaches to understand the critical pathways to toxicity. With the advances in the various “omics” technologies progressing at a rapid pace, the quest for better predictive models of human toxicology and efficacy will speed up the practical application of these technologies in determining human tissue specific responses to drugs and environmental agents. The completion of the human genome sequence and the evolution of the tools for analysis of gene expression, proteomics, and metabolomics have resulted in the generation of massive data sets on relevant pathways and molecular biology. This “explosion” of information in identifying new genes and gene interactions has created a major challenge in the biologically meaningful interpretation of this data. Bioinformatics tools are also rapidly evolving to assist in making biologically relevant interpretations of these complex data. Understanding agent induced pathway perturbations will also result in a better understanding of efficacy pathways. The outcome of the coordinated efforts will result in improved approaches to personalized medicine and reduced environmental risk. This session will focus on current and future approaches to implement the “vision and strategy” with a focus on human cell model systems, “omics”, and bioinformatics.

10:30 Introduction (E. Elmore and B. L. Upham)
10:40 A-7 Toxicity Pathways, In Vitro Assays, and Computational Cell Biology: Using the Best Science for Toxicity Testing and Risk Assessment
Melvin E. Andersen, The Hamner Institutes for Health Sciences
11:05 A-8 Mechanistic Insight into Drug-induced Organ Injury with Comparisons of Animal and Human Tissue
Alison E. M. Vickers, Allergan
11:30 A-9 Knowledge Profile Aporoach: Insights Into Drug Action and Toxicity Mechanisms
Nikolai Daraselia, Ariadne Genomics
11:55 A-10 Using Gene Expression and Pathway Analysis for Efficacy and Toxicity Assessment
Eugene Elmore, University of California – Irvine
12:20 Discussion

TREE BIOTECHNOLOGY

Conveners:       Shujun Chang, ArborGen, LLC, and Scott Merkle, University of Georgia

10:30 am – 12:30 pm Plant Symposium Ballroom A

Forest trees are important resources for the world. As forested land area continues to decline at the rate of about 7.7 million ha/year due mainly to conversion to agriculture or urbanization, purpose-grown, highly-productive forest plantations have become an increasingly important source of wood and other forest products. Building upon the gains made via conventional breeding and silvicultural practices, biotechnology offers an exciting opportunity to optimize the potential of trees and to bring quality and productivity to an unprecedented level. In this session, we intend to highlight recent, exciting developments in tree biotechnology. Speakers will present their research demonstrating recent developments in tree genomics, gene discovery, and gene function analysis and product development through mass clonal propagation and genetic transformation.

10:30 Introduction (S. Chang and S. Merkle)
10:35 P-8 Freeze-tolerant Eucalyptus as a Renewable Feedstock for Industrial Applications
Maud Hinchee, ArborGen LLC
10:55 P-9 Populus Genomics, Candidate Gene Identification and Accelerated Domestication
Gerald A. Tuskan, Oak Ridge National Lab
11:15 P-10 Cisgenic and Intragenic Approaches to Genetic Modification of Growth and Form in Poplar
Steven Strauss, Oregon State University
11:35 P-11 Phenylpropanoid Networking in Populus
Chung-Jui Tsai, University of Georgia
11:55 P-12 Hardwood Tree Biotech Advances in Southeast
Scott Merkle, University of Georgia
12:15 Discussion

STUDENT NETWORKING LUNCHEON

Conveners:       Phillip J. Kauth, University of Florida, and S. Richelle Monaghan, University of Waterloo

12:30 pm – 2:00 pm Education Symposium Meeting Rooms 8&9

Young scientists and their advisors are invited to attend this luncheon to discuss what they learned from Saturday and Sunday’s workshops.  This will also be an excellent opportunity for attendees to further network with the speakers and mock interviewers from Sunday night.  A final goal is to determine what other areas of career development are necessary to the young SIVB scientist and could be focused on for the 2009 Student Committee hosted session.

12:30 Introduction (P. J. Kauth and S. R. Monaghan)
12:40 E-2 A Government Scientist: How It Works with the Agricultural Research Service
David Ellis,
USDA/ARS

CANCER BIOLOGY AND CELL-CELL INTERACTIONS

Moderator:        Brad L. Upham, Michigan State University

1:30 pm – 2:30 pm Animal Interactive Poster Session Ballroom B&C
A-2000 The Role of Various Phospholipase A2 Enzymes in Key Cell Signaling Events Affiliated with Tumor Promotion
Brad L. Upham, Michigan State University, J. S. Park, P. Babica, O. Adamovsky, and J. E. Trosko
A-2001 The Established Insect Cell Line, BCIRL-HzAM1, Expresses a Cellular and a Secretory Phospholipase A2
Cynthia L. Goodman, USDA/ARS/BCIRL, and D. Stanley
A-2002 Tumor Promotion-relevant Cell Signaling: Key Roles of Annexins and Phospholipases
Pavel Babica, Michigan State University, J. S. Park, I. Sovadinova, L. Blaha, D. A. Whitten, C. G. Wilkerson, J. E. Trosko, and B. L. Upham
A-2003 Differential Expression of Full-length and Truncated Cullin-5 is Associated with Granulocytic Differentiation of HL-60 Leukemia Cells
Michael J. Fay, Midwestern University, G. K. Tan, S. S. Baxter, M. L. Hall, A. M. S. Mayer, and L. A. Carlson
A-2004 Effects of 5-Fluorouracil Treatment on MicroRNA Expression in MCF-7 Breast Cancer Cells
Maitri Shah, East Carolina University, Mary A. Farwell, and Baohong Zhang
A-2005 The Effect of Green Tea Polyphenon 60 Treatment on miRNA Expression in MCF7 Breast Cancer Cells
Lindsey N. Fix, East Carolina University,Mary A. Farwell, and Baohong Zhang
A-2006 Development of Organ Culture Conditions for Maintenance of Normal and Malignant Human Colon Tissue
Narasimharao V. Bhagavathula, University of Michigan, M. K. Dame, M. N. Aslam, and J. Varani

CELLULAR MODELS – POLICY, IMMUNITY, AND TOXICOLOGY

Moderator:        Elizabeth J. Roemer, State University of New York – Stony Brook

1:30 pm – 2:30 pm Animal Interactive Poster Session Ballroom B&C
A-2007 The Canadian Council on Animal Care: Replacement, Reduction, Refinement Alternatives Online
Gilly Griffin, Canadian Council on Animal Care, and Nicole Fenwick
A-2008 Efficacy Testing of a Novel Retinoid in Gottingen Mini-pig Skin Organ Culture Yields Comparable Results to That in Intact Animal
Michael K. Dame, The University of Michigan Medical School, T. Paruchuri, M. DaSilva, N. Bhagavathula, W. Ridder, and J. Varani
A-2009 Healing of Dermal Wounds in the EpiDerm-FT In Vitro Human Skin Model
Patrick Hayden, MatTek Corporation, G. Stolper, C. Cooney, and M. Klausner
A-2010 Use of Cultured Human Skin Cells and a Skin Tissue Construct as Models to Study the Genotoxic Marker γ-H2AX Following Sulfur Mustard Exposure
A. L. Miller, United States Army Medical Research Institute of Chemical Defense, C. L. Gross, E. W. Nealley, O. E. Clark, N. K. Waraich, K. L. Rodgers, and W. J. Smith
A-2011 Human Bronchial Epithelial Cells as a Model for Human Lung Airway Injury and Therapeutic Intervention Following Sulfur Mustard Exposure In Vitro
O. E. Clark,
United States Army Medical Research Institute of Chemical Defense, E. W. Nealley, A. L. Miller, Y.-S. Jung, and W. J. Smith
A-2012 Lack of Genotoxicity in Cultured Human Small Airway and Microvascular Cells Following Exposure to the Nerve Agent, VX
C. L. Gross, United States Army Medical Research Institute of Chemical Defense, E. W. Nealley, A. L. Miller, M. T. Nipwoda, O. E. Clark, and W. J. Smith
A-2013 Comparison of Fish Cell Line Responses to Chemicals and Process-affected Waters from the Oil Sands of Alberta, Canada
Bryan Sansom, University of Waterloo, R. Kavanagh, M. MacKinnon, D. G. Dixon, and L. E. J. Lee

IN VITRO BIOLOGY

Moderator:        Michael Spencer, Monsanto Company

1:30 pm – 2:30 pm Plant Interactive Poster Session Ballroom B&C
P-2000 Low-cost Methods for Production of Commercial-size Fruit from Sugar-loaf Pineapple (Anasas comosus) Plantlets in Jamaica
Sylvia A. Mitchell, University of the West Indies, M. H. Ahmad
P-2001 Macronutrient Optimization for In Vitro Growth of Turmeric (Curcurma longa L.)
Sean Michael Halloran, Clemson University, and Jeffrey Adelberg
P-2002 Secondary Metabolism Inducing Treatments During In Vitro Development of Turmeric (Curcuma longa L.) Rhizomes
Matthew M. Cousins, Clemson University, Jeffrey Adelberg, Feng Chen and James Reick
P-2003 The Use of Silver Compounds to Direct Plant Tissue Culture Development
Benjamin Steinitz, The Volcani Center, Y. Tabib, N. Bar, and N. Bernstein
P-2004 Obtaining Sethoxydim Resistance in Seashore Paspalum (Paspalum vaginatum) Via In Vitro Selection
Douglas L. Heckart, University of Georgia, W. Parrot, and P. Raymer

 

TRANSFORMATION TECHNOLOGY

Moderator:        Lisa Lee, The Scotts Miracle-Gro Company

1:30 pm – 2:30 pm Plant Interactive Poster Session Ballroom B&C
 P-2005  Rice Promoters That Confer Organ-specific Transgene Expression
Roger Thilmony, USDA/ARS Western Regional Research Center, Mara E. Guttman, Meridith Cook, and James G. Thomson
 P-2006  Identification of Parameters Influencing Agrobacterium-mediated Transformation of Peanut
N’NAn A. S. Diby, Alabama A&M University, K. Konan, and H. Dodo
 P-2007  Comparison Between Mannose and Kanamycin Selection Systems for Genetic Transformation of Citrus
Manjul Dutt, University of Florida-IFAS, and J. W. Grosser
 P-2008  Site-specific Excision of Targeted DNA Is Facilitated by Bxb1 in the Arabidopsis Genome
James Thomson, USDA Western Regional Center, Y. Y. Yau, R. Blanvillain, and D. Ow
 P-2009  Induction of nptII Exogene Removal from Transgenic Grape (Vitis vinifera ‘Brachetto’
Lucia Martinelli, IASMA Research Center, L. Dalla Costa, I. Vaccari, V. Poletti, and M. Mandolini

 Monday, June 8
Even Poster Authors will be present
2:30 pm – 3:30 pm

 

CHALLENGES TO LARGE SCALE LIQUID PLANT MICROPROPAGATION

Conveners:       Jeffrey W. Adelberg, Clemson University and Ebrahim Firoozabady, Del Monte Fresh Produce Company

3:30 pm – 5:00 pm Plant Symposium Meeting Rooms 8&9

Micropropagation is often limited to high value ornamental plants and found too costly for large-scale industrial crops.  Almost all micropropagation is done on semi-solid medium despite greater efficiencies of liquid media propagation: lower materials cost and less preparation time, reduced labor during transfer – clean room and greenhouse, greater plant growth due to better uptake of nutrients, and greater opportunities for automation in scale-up.  Considerable barriers, however, may prevent implementation of liquid culture in large scale operations.  These include the possibility of hyperhydricity, a lack of equipment and process, the increased risk of contamination in large vessels, and costly investment in new vessels with mechanized aeration.  In this symposium, speakers will share their experiences developing commercially scaled liquid micropropagation systems with value added germplasm.

3:30 Introduction (J. W. Adelberg)
3:35 P-13 Introduction to Large Scale Liquid Micropropagation Systems
Jeffrey W. Adelberg, Clemson University
3:55 P-14 Transgenic Loblolly Pine and the Importance of Somatic Embryogenesis Scale-up Technologies for Future Commercialization
Shujun Chang, ArborGen, LLC
4:15 P-15 Challenges to Large Scale Liquid Plant Micropropagation – the Arundo Story
Laszlo Marton, University of South Carolina
4:35 P-16 The Utilization of Temporary Immersion System (RITA) to Enhance Initial Steps in Commercial Micropropagation of Cordyline spp.
Cuauhtemoc Navarro, Agromod
4:55 Discussion

COMMERCIALLY AVAILABLE IN VITRO 3-D TISSUE MODELS FOR BIOMEDICAL RESEARCH AND PRODUCT DEVELOPMENT

Convener:         Elizabeth J. Roemer, State University of New York – Stony Brook

3:30 pm – 5:00 pm Animal Symposium Meeting Room 1

Cells inhabit three-dimensional (3-D) space when tissues are growing in vivo. When cells are grown in vitro as 3-D constructs they better mimic in vivo tissue. 3-D models can be manipulated through the use of multiple cell types, matrices, and culture conditions to yield constructs with histological and physiological profiles closely matched to those found in the body. Through recreation of complex mechanical and biochemical microenvironments, these systems are proving increasingly valuable for multiple applications including studies of pathological and normal physiological processes, pre-clinical drug development and in vitro toxicology. 3-D models are also key alternatives to animal testing. While it is possible to grow 3-D models in the research lab, the option of purchasing ready-to-use constructs is often attractive. This workshop will explore a range of commercially available models.

3:30 Introduction (E. J. Roemer)
3:35 A-11 3-D Tissue Models in Contract Research: Points to Consider for Efficacy, Product Development, or Regulatory Testing Programs.
Hans Raabe, Institute for In Vitro Sciences, Inc.
4:00 A-12 Customized 3D Human Tissues for Discovery
Christophe Egles, Tufts University School of Dental Medicine
4:25 A-13 Commercial Production of In Vitro Human Epithelial Models at MatTek Corporation: A Survey of Available Models and Applications
Patrick Hayden, MatTek Corporation
4:50 Discussion

PLANT TRANSGENE EXPRESSION SYSTEMS

Convener:         Allan R. Wenck, BASF Plant Systems, LLC

3:30 pm – 5:00 pm Plant Symposium Ballroom A

Technology is rapidly evolving to deliver improved and alternative methods for currently utilized plant transgene expression systems. One gene may no longer be enough. In addition, methods to fine-tune expression of transgenes and even endogenous genes are required. Further, multi-gene solutions and even whole metabolic pathways are increasingly being seen as required for transgenic solutions. In this session several different possibilities will be explored. New modular vectors for Agrobacterium mediated transformation will be discussed. Technology to better control transgenes and even endogenous genes will be presented. Finally, the potential for engineering complete plant artificial chromosomes will be explored.

3:30 P-17 Modular Assembly of Multi Gene Plant Transformation Vectors
Tzvi Tzfira, University of Michigan
4:00 P-18 Editing the Genome of Crop Plants with Engineered Zinc Finger Proteins
Philip D. Gregory, Sangamo BioSciences
4:30 P-19 Engineered Minichromosomes in Maize
James A. Birchler, University of Missouri

BIOASSAY PROTOCOLS

Convener:          Dennis A. Laska, Eli Lilly & Company

5:00 pm – 6:00 pm  Animal Workshop Meeting Room 1

Development and validation of reliable bioassays is critical in the consumer product and pharmaceutical industries, as well as for environmental monitoring and bio-defense. This workshop will evaluate the basic requirements and expectations of bioassay development in a round table discussion following a featured presentation by Dr. Pamela Morris and Ms. Maria Vizcaino from the Hollings Marine Laboratory (Charleston, SC).

5:00 Introduction (D. A. Laska)
5:05 A-14 Determining the Antimicrobial Activity of Bacteria Associated with a Caribbean Coral
Pamela J. Morris and Maria I. Vizcaino, Hollings Marine Laboratory
5:45 Panel Discussion
Panelists:
John W. Harbell, Mary Kay Corporation
William J. Smith, US Army Medical Research Inst of Chem Defense
Amy A. Wang, GlaxoSmithKline

GRAPHICAL AND STATISTICAL SOFTWARE AND ANIMATED VISUALIZATIONS

Convener:          Jeffrey W. Adelberg, Clemson University

5:00 pm – 6:00 pm  Plant Workshop Ballroom A

Graphical visualization of data from multivariate analyses allows us to explore the statistical, and perhaps cryptic relationships, among treatments. The workshop will 1) show strategies and methods for designing in vitro experimentation where multiple factors (e.g., media inputs and environmental conditions) and responses (e.g., growth and development) are varied; 2) analyze with the objective of optimizing one or multiple responses; and 3) present the data analysis in highly graphical formats (static and dynamic) that capture the most important factor/response relationships. The overall approach is commonly used in process engineering, but has particular relevance to in vitro biology experimentation.

5:00 Introduction (J. W. Adelberg)
5:05 P-20 Design of Multivariate Experiments
William C. Bridges, Clemson University
5:20 P-21 Optimizing Macro-nutrients for Stage II and Stage III of Micropropagation Using Response Surface Methodology
Jeffrey W. Adelberg, Clemson University
5:35 P-22 Visualization, Interpretation, and Mining of Data from Multivariate Designs
Randall P. Niedz, USDA/ARS
5:50 Discussion

MARINE INVERTEBRATE AND FISH CELL LINE ESTABLISHMENT

Convener:         James J. Grasela, USDA/ARS/BCIRL

5:00 pm – 6:00 pm  Animal Roundtable Meeting Room 3

This roundtable discussion aims to provide information on recent developments in marine invertebrate and fish cell culture and their potential uses and applications. This session will focus on in vitro methodologies for developing aquatic animal cell cultures in general, and specific requirements for marine invertebrate and fish tissue culture. Other topics to be discussed will include the mechanistic approaches for immortalizing aquatic invertebrate cells, and the potential uses of marine invertebrate and fish cell lines in aquatic animal health, biotechnology, and environmental monitoring.

Panelists:
David Barnes, Mount Desert Island Biological Laboratory
Lucy E. J. Lee, Wilfrid Laurier University
Shirley A. Pomponi, Harbor Branch Oceanographic Institution
Guy Smagghe, Ghent University

 

PLANT BIOTECHNOLOGY CONTRIBUTED PAPER SESSION

Moderator:        John J. Finer,    OARDC/The Ohio State University

5:00 pm – 6:00 pm Plant Contributed Paper Session Meeting Rooms 8&9
 5:00  P-1012 Green Genetic Engineering Technology: Rearrangement of Endogenous Functional Genetic Elements to Create Improved Grapvines
Dennis J. Gray, University of Florida-IFAS, Z. T. Li, S. A. Dhekney, D. L. Hopkins, and T. W. Zimmerman
 5:15  P-1013 Facilitation of GFP Visualization in Green Tissues Using Bleaching Herbicides
John J. Finer
, OARDC/The Ohio State University
 5:30  P-1014 Over Expression of AtNHX1 Gene in Transgenic Salt Tolerant Cultivated Tomato
Nanna Rama Swamy, Kakatiya University, Praveen Mamidala, and Hongxia Zhang
 5:45  P-1015 Qualitative Analysis of Aloe Vera: Commercially Important Medicinal Plant Through HPLC and Clonal Propagation of ChemoProfiled Material Through In Vitro Techniques
Shailendra K. Tiwari, State Forest Research Institute, P. K. Shukla, Amit Pandey, S. Mishra, M. P. Goswami, and P. Bhargava