Wednesday, June 18

2008_Tuscon

Daily Program
Abstracts & Posters
Saturday, June 14 Keynote Symposium Plant Contributed Papers
Sunday, June 15 Plenary Symposia Animal Posters
Monday, June 16 Animal Symposia Plant Posters
Tuesday, June 17 Plant Symposia Education Posters
Wednesday, June 18 Invertebrate Conference Symposia 2008 Abstract Index
Education Symposia Late Submission Abstracts
Animal Contributed Papers

WEDNESDAY, JUNE 18

7:00 am – 12:30 pm
Registration Turquoise Foyer


BIOFUELS – A RIPE FIELD FOR RESEARCH OR NOT?

Conveners:        Sylvia A. Mitchell, University of the West Indies and David D. Songstad, Monsanto

8:00 am – 10:00 pm
Plenary Symposium Presidio I and II

Obtaining fuels from living plants, rather than plants turned into to fuel many eons ago, appears to have become necessary if we are going to continue to fuel development and prosperity. Some of the issues to consider include: what is the historical background to biofuel production?, what plant species to use?, do we limit the plant species used to non-food plants?, how do we obtain economical levels of biofuel from plant species?, which countries should be involved and how?, do we use prime land or can we use marginal land?, what are the best practices at present and how can we learn from them?, what is the best way forward?. The speakers have been chosen from a variety of backgrounds and will present recent data to allow for discussion of these considerations and identify some ways forward – for research and for development.

8:00 Introduction (P. M. Pijut)
8:05 PS-11 The Impact of Improved Traits and Genetics on Biofuel Production
Michael Edgerton, Monsanto Company
8:40 PS-12 70 Years of Lessons on Biofuel Production from Brazil
Luciano Nass, USDA-ARS, and David Ellis, USDA-ARS
9:15 PS-13 Biofuel Development in the Caribbean – The Pros and Cons
Sylvia Adjoa Mitchell, University of the West Indies
9:35 Wrap Up
9:45 Discussion

 

10:00 am – 10:30 am
Coffee Break Turquoise Ballroom Foyer

 

EMERGING TECHNOLOGIES

Convener:         Linda B. Jacobsen, Berit Biotech, LLC

10:30 am – 12:00 pm
Animal Symposium Coronado I

Functional assays are measured on cultured cells at one or a few time points after some precipitating event such as transfection with a nucleic acid, addition of drug, or time after plating. The assay time (4 hours, 24 hours, 48 hours, 1 week etc.) is often selected empirically based on the results of the functional assay in treated and control cultures. The time selected may be when there is maximal difference between treated and control cultures without regard for other biological changes occurring in the cells. This session will present new non-invasive technologies that permit measurement of the biological state of the living cells before, during and after the treatment to aid in better selecting time points for analysis, and understanding the other events happening in the cells during the experiments. Additionally these technologies can be used for direct study of effects of drugs and other biological materials. The technologies to be presented are distinctly different, one measuring electrical impedance, and the other measuring oxygen consumption rate and extracellular acidification rate. The third presentation in this session will be a new micro-incubator concept in which living cells can be viewed and tested without effects caused by removal from an incubator.  Attend this session to learn how measurements in living cells in 24-well, 96-well cultures and in micro-incubators can provide you new understandings of the kinetics in your cultures during the experiment resulting in better experiments and data interpretation.

10:30 Introduction (L. B. Jacobsen)
10:35 A-18 Using Cell Sensor Impedance Technology for Label-free and Real-time Cell-based Assays
Yama Abassi, ACEA Biosciences Inc.
11:00 A-19 Extracellular Flux Measurements Provide a New Window on Cellular Bioenergetics
George Rogers, Seahorse BioScience
11:25 A-20 A Hybrid CMOS/PDMS Microsystem for Autonomous Cell Culture and Incubation
Jennifer Blain Christien, Arizona State University
11:50 Discussion

 

PLANT MODIFICATION FOR INCREASED BIOFUEL PRODUCTION

Convener:         Zeng-Yu Wang, The Samuel Roberts Noble Foundation

10:30 am – 12:00 pm
Plant Symposium Presidio I and II

Plant biomass has the potential to play a major role in the substitution of fossil fuels with renewable resources. Biomass available for energy on a sustainable basis includes herbaceous crops and woody species. To date, most ethanol is derived from starch or sugar crops by fermentation. The ability to produce cellulosic ethanol from low-cost biomass will be key in making biofuel competitive with gasoline. The energy in lignocellulosic biomass is largely in plant cell walls. Cell wall recalcitrance has been identified as a major limitation to the economic production of ethanol from plant biomass. The cost of ethanol production from lignocellulosic materials is relatively high based on current conversion technologies; the main challenge is the low yield and high cost of the pretreatment/hydrolysis process. Speakers in this session will highlight recent advances in using biotechnological approaches to improve biofuel production from different biomass crops. The new approaches include genetic engineering of metabolic pathways, reduction of cell wall recalcitrance and improvement of biomass production.

10:30 Introduction (Z.-Y. Wang)
10:35 P-30 Genetic Improvement of Dedicated Energy Crops
Steven R. Thomas, Ceres Inc.
10:55 P-31 Genetic Manipulation of Lignin Biosynthesis to Improve Biomass Characteristics for Agro-industrial Processes
Fang Chen,The Samuel Roberts Noble Foundation
11:15 P-32 Modifying the Corn Genome to Improve Its Biomass Biofuel Production
Sanghyuck Park, Michigan State University
11:35 P-33 Agrobacteriummediated Transformation of Switchgrass
Zeng-Yu Wang, The Samuel Roberts Noble Foundation
11:55 Discussion

 

PLANT TRANSFORMATION

Moderator:        Dennis J. Gray, University of Florida

10:30 am – 12:30 pm
Plant Contributed Paper Session Presidio III and IV
10:30 P-1012 Overexpression of the Arabidopsis Transcription Factor REVOLUTA Leads to Increased Soybean Seed Size and Yield
Rugang Li, Targeted Growth, Bonnie Bancroft, Kristina Lum, Thu Nguyen, Jay De Rocher, and Daina Simmonds
10:45 P-1013 Field Testing Transgenic Grapevine for Disease Resistance
Dennis J. Gray, University of Florida, Z. T. Li, S. A. Dhekney, D. L. Hopkins, and T. W. Zimmerman
11:00 P-1014 Overcoming Obstacles to Genetic Transformation in Vitis
Sadanand A. Dhekney, University of Florida, Z. T. Li, T. W. Zimmerman, and D. J. Gray
11:15 P-1015 Camelina sativa Transformation by Floral Dip and Simple Large-scale Screening of Markerless Transformants
Xunjia Liu, Targeted Growth Canada, Sharon Leung, Jennifer Brost, Suzanne Rooke, and Thu Nguyen
11:30 P-1016 Comparative Analysis of Diploid and Polyploid Buffalograss Based on Transient Gene Expression and In Vitro Regeneration
Hikmet Budak, Sabanci University
11:45 P-1017 Genetic Transformation in Diploid Turkish Brachypodium distachyon Based on a Well-established Tissue Culture System
Bahar Sogutmaz Ozdemir, Sabanci University, and H. Budak
12:00 P-1018 An Efficient Protocol for Stable Transformation of Glycin max L. and Capsicum annuum L. by Agroinjection
Zia Muhammad, Quaid-i-Azam University
12:15 P-1019 Enhancing Agrobacterium-mediated Transformation Efficiency of Sugarcane: Progress Towards an Efficient, Genotype-independent Method
Harjeet Kaur Khanna, Queensland University of Technology, M. Bokan, M. Harrison, L. Kancherela, M. B. Dickman, and J. L. Dale

 

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