2008_Tuscon

Daily Program
Abstracts & Posters
Saturday, June 14 Keynote Symposium Plant Contributed Papers
Sunday, June 15 Plenary Symposia Animal Posters
Monday, June 16 Animal Symposia Plant Posters
Tuesday, June 17 Plant Symposia Education Posters
Wednesday, June 18 Invertebrate Conference Symposia 2008 Abstract Index
Education Symposia Late Submission Abstracts
Animal Contributed Papers

TUESDAY, JUNE 9

7:00 am – 5:00 pm Registration Presidio Registration Area

 

LARGE SCALE, CELL AND TISSUE-BASED PRODUCTION OF BIOPHARMACEUTICALS AND SECONDARY METABOLITES

Conveners:        Haruhiko Tsumura, Kirin Pharma Co., and Pamela J. Weathers, Arkansas Bioscience Institute

8:00 am – 10:00 am Plenary Symposium Presidio I and II

This session will cover relevant aspects of production of biopharmaceutical and secondary metabolites by animal cells and plant cells and tissues in large scale.  The development of production cell lines, culture medium, metabolic engineering, the use of genomic tools, bioreactor design and engineering, and some case studies of actual commercial production will be addressed. Where appropriate, speakers will address issues of economics. Attendees will benefit by learning about progress made in novel approaches to making bioproducts less expensive through innovation and how the correct decisions in large scale culture can improve downstream processing as illustrated by some success stories, along with how to avoid some of the pitfalls.

8:00 Introduction (H. Tsumura and P. J. Weathers)
8:05 PS-7 Animal and Plant Cultures: Production of Biopharmaceuticals and Secondary Metabolites
Wayne Curtis, Pennsylvania State University
8:35 PS-8 Process Development for mAb Therapeutic Production in 10,000 L-reactors with CHO Cells
Chikashi Hirashima, Chugai Pharmaceutical
9:00 PS-9 Air Lift Balloon Type Bioreactor: Platform for Commercial Production of Plant Based Small Molecules and Tissues
Ganapathy Sivakumar, Arkansas State University
9:20 PS-10 Novel Plant Reactors for Pharmaceuticals Production
Chunzhao Liu, Chinese Academy of Sciences
10:00 am – 10:30 am Coffee Break Turquoise Ballroom

 

HIGHLIGHT OF PLANT GENOMICS RESEARCH AT THE UNIVERSITY OF ARIZONA

Convener:          Wayne Parrott, University of Georgia

10:30 am – 12:30 pm Plant Symposium Presidio III and IV

The term genomics was adopted in the late 1980’s to encompass all aspects of mapping, sequencing and analysis of information of an organism’s entire genome. Today, the field also includes the characterization of genes, mRNAs and protein products (functional genomics), the study of DNA and histone modification patterns (epigenomics) and the elucidation of evolutionary relationships between genomes of different species (comparative genomics). Scientists at the University of Arizona had greatly contributed to the advancement of all aspects of plant genomics research. This session will highlight pioneer work unraveling the molecular basis of paramutation, the use of RNAi as a functional genomics tool and the study of evolution in plants using comparative genomics.

10:30 Introduction (L. B. Jacobsen and E. J. Roemer)
10:35 P-23 Interchromosomal Transfer of Epigenetic Information
Vicki L. Chandler, University of Arizona
11:10 P-24 The iPlant Collaborative: A Cyberinfrastructure-Centered Community for a New Plant Biology
Richard A. Jorgensen, University of Arizona
11:45 P-25 The Oryza Map Alignment Project: Genomes in Flux
Rod A. Wing, Arizona Genomics Institute
12:20 Discussion

 

STEM CELLS, PLASTICITY, AND REGENERATIVE MEDICINE

Conveners:        Paul J. Price, D-Finitive Cell Technologies, and Raziel S. Hakim, Howard University

10:30 am – 12:30 pm Animal Symposium Coronado I

Stem cells have long been recognized as the source of mature differentiated cells in embryonic as well as mature organ systems. While the terms totipotent, pleuripotent and multipotent have been used to describe stem cells by the number of different mature cell types they can become, evidence is accumulating that the culture environment in which cells are held can not only affect the range of cell types that stem cells can become, but can even cause reprogramming of mature cells.   The term transdifferentiation refers to mature cells which have been reprogrammed by environmental factors to new fates. Current research indicates that stem and even mature cells of post-natal origin can play a key role in cell-based therapies. The 3 speakers will discuss their research results with different sources of post-natal stem cells.

10:30 Introduction (P. J. Price and R. S. Hakim)
10:35 A-12 Mesenchymal Stem Cells and the Development of Therapeutics
Michelle Greene, Millipore Corporation
11:10 A-13 Mesenchymal Progenitors Able to Differentiate into Osteogenic, Chondrogenic, and/or Adipogenic Cells In Vitro are Present in Most Primary Fibroblast-like Cell Populations
Kazuhiro Sudo, Riken Bio Resource Center
11:45 A-14 Induction of Hepatocyte-like Cells from Mesenchymal Stem Cells and the Transplantation into Liver-injured Rats
Kiyohito Yagi, Osaka University
12:20 Discussion

Tuesday, June 17
Odd Poster Authors will be present
1:30 pm – 2:30 pm

 

ANIMAL CELL BIOTECHNOLOGY

Moderator:         Kim O’Connor, Tulane University

1:30 pm – 3:30 pm Animal Contributed Paper Session Coronado I
 1:30  A-1006 Multiendpoint Mechanistic Profiling of Hepatotoxicants in HepG2/C3A Human Hepatoma Cells and Comparison of Statistical Methods for Development of a Prediction Model for Acute Hepatotoxicity
Thomas J. Flynn, US FDA, and Martine S. Ferguson
 1:50  A-1007 In Vitro Cellular Response to Nanoparticle Exposure
Julie Elaine Morgan, Clayton State University, and J. A. Jordan
 2:10  A-1008 Computational Tissue Engineering: Monte-Carlo Simulation of Restructuring Dynamics During Tissue Self-assembly of Prostate Cancer Spheroids
Kim C. O’Connor, Tulane University, and H. Song
 2:30  A-1009 Tools for Genetic Characterization and Identification of Cell Lines
Manohar Rajeev Furtado, Applied Biosystems, R. Fang, J. G. Shewale, and F. Hyland
 2:50  A-1010  Mapping Signaling Pathways That Control Gap Junction Function Using Modern Proteomic Approaches
Brad Luther Upham, Michigan State University, D. A. Whitten, C. G. Wiklerson, J. S. Park, I. Sovadinova, P. Babica, J. E. Trosko, and L. Blaha
3:10  A-1011  Online Monitoring of Physiological Parameters of Cell Cultures
Ralf Ehret, Bionas GmbH, Elke Thedinga, Sabine Drechsler, Axel Kob, Marcus Wego, Sebastian Rost, Steffen Fürst, and Werner Baumann

 

STEM CELL BIOLOGY AND CANCER DRUG DEVELOPMENT

Moderator:         Lia H. Campbell, Cell and Tissue Systems, Inc.

2:30 pm – 3:30 pm Animal Interactive Poster Session Turquoise Ballroom
A-2008 Retaining Cell Integrity During Organotypic Model Viability Assays: Alternatives to MTT
Catherine R. Kavanagh, State University of New York-Stony Brook, L. J. Crawford, K. M. Sawicka, S. R. Simon, and E. J. Roemer
A-2009 In Vitro Investigation of Antioxidant and Antiproteolytic Properties of the Clove Extracts: Tellimagrandin I and Casuarictin
Sumaira Zamurrad, State University of New York-Stony Brook, S. Parrino, F. Daccueil, E. J. Roemer, and S. R. Simon
A-2010 Optimization of a New Method of Characterizing Live Basal Keratinocytes Using Chariot Transfection Reagent
Yusuke Fukuda, State University of New York-Stony Brook, E. J. Roemer, S. R. Simon, and M. Matic
A-2011 Comparison of Growth Characteristics, Neurochemical Parameters and Response to Toxicants for Neural Tissue Derived Cell Lines from Goldfish and from Crayfish
Lucila E. J. Lee, Wilfrid Laurier University, M. R. Bufalino, and M. P. Wilkie
A-2012 Assessing the In Vitro Respiratory Toxicity of Fine Particles of Al2O3 and SiO2: a Precursor Study for Lunar Dust Toxicity
Jacqueline A. Jordan, Clayton State University, A. M. Verhoff, and D. G. Fischer
A-2013 D-Glucose Protection Against MPP+ Induced Cell Death in Human Lung Carcinoma A549 Cell Line
David Fouad Elmashat, Florida A&M University, Ramesh B. Badisa, and Karam F. Soliman

 

PLANT TRANSFORMATION, RNAi, AND PHARMACEUTICAL/VALUE ADDED TRAITS

Moderator:         Sandra L. Kelly, Agriculture and Agri-FoodCanada

2:30 pm – 3:30 pm Plant Interactive Poster Session Turquoise Ballroom
P-2006 Phloem Specific Transgene Expression Driven by AtSUC2 Gene Promoter in Transgenic Citrus Plants to Develop Citrus Greening Resistance
Ahmad Al-Sayed Omar, University of Florida, Manjul Dutt, Gary Barthe, Vladimir Orbovic, and Jude Grosser
P-2007 Genetic Manipulation for Enhancing Calcium Uptake in Lettuce
Sung Hun Park, Kansas State University, M. P Elless, J. Park, W. Lim, and K. D. Hirschi
P-2008 Optimization of Transformation Efficiency in Flax
Sandra L. Kelly, Agriculture & Agri-Food Canada, and Mark C. Jordan
P-2009 Base-by-Base Analysis of siRNA Production by a Plant Transgene
Victor Gaba, ARO Volcani Center, Y. M. Shiboleth, S. Singer, E. Kukurt, D. Liebmann, L. Maslenin, A. Rosner, and A. Gal-On
P-2010 Wheat Virus Resistance Via Interference RNA
Luisa F. Cruz, Kansas State University, John P. Fellers, and Harold N. Trick
P-2011 Partial Characterization and Purification of Plant Derived Butyrylcholinesterase to Treat Organophosphate Poisoning
Latha Kannan, Arizona State University B. C. Geyer, P.-E. Garnaud, R. R. Woods, M. Muralidharan, I Cherni, and T. S. Mor

 

IN VITRO TOOLS, TECHNIQUES, AND OPTIMIZATION

Moderator:         Michael E. Kane, University of Florida

3:30 pm – 5:00 pm Plant Contributed Paper Session Presidio I and II
 3:30  P-1006 Seed Physiology of Bletia purpurea (Pine Pink; Orchidaceae)—Fluctuating Low Temperature and Dark Slow Development and Inhibit Germination
Timothy R. Johnson, University of Florida, M. E. Kane, and H. E. Perez
 3:45  P-1007 Asymbiotic Seed Germination and In Vitro Seedling Development of Cyrtopodium punctatum: A Propagation Protocol for an Endangered Florida Native Orchid
Daniela Dutra, University of Florida, M. E. Kane, and L. Richardson
 4:00  P-1008 Synergistic Effect of Auxin and Cytokinin on In Vitro Androgenesis in Azadirachta indica A. Juss
Rakhi Chaturvedi, Indian Institute of Technology-Guwahati, and Priyanka Srivastava
 4:15  P-1009 Standardization of Protocol for Efficient In Vitro Clonal Propagation of Rare Medicinal Plants e.g., Elaeocarpus & Capparis
L. N. Shukla, B. R. Ambedkar Bihar University, C. P. Shukla, B. K. Mishra, Manoj Kumar, Sushma Kumari, and T. Upadhyay
 4:30  P-1010 Germplasm Evaluation of Andographis paniculata (Kalmegh) Through Chemoprofiling for In Vitro Mass Multiplication of Quality Germplasm from Satpura Plateau Region of Madhya Pradesh
Shailendra Kumar Tiwari, State Forest Research Institute, Vijay Bahadur, Amit Pandey, Shweta Mishra, M. P. Goswani, and Pankaj Bhargava
 4:15  P-1011 Micropropagation of Boerhaavia diffusa – A Valuable Medicinal Plant
Regha P. Periyannan, Muthyammal College of Arts and Sciences, N. Vinod Kumar, M. S. Kavitha, M. Rajasekara Pandian, and E. G. Wesely

 

NEW STRATEGIES FOR PRODUCTION OF SPECIALIZED METABOLITES

Convener:          Fabricio Medina Bolivar, ABI/Arkansas State University

3:30 pm – 5:00 pm Plant Symposium Presidio III and IV

Plants have evolved specialized networks for the biosynthesis of a rich repertoire of natural products. These complex molecules serve as chemical languages in ecosystems, and often confer protective characteristics to plants allowing them to survive, and prosper in a multitude of challenging ecological niches. Specialized metabolism is an economically important source of fine chemicals, such as medicines, insecticides, dyes, flavors, and fragrances. Scientists have made significant progress at developing strategies to study and alter specialized metabolism. In particular, genomics and metabolomics approaches are leading to an advanced understanding of how these metabolic networks function in a coordinated fashion leading to desired traits or phenotypes. This symposium will provide an update on novel strategies used by multidisciplinary teams to harness and alter biosynthetic pathways for the production of natural products with diverse bioactivities.

3:30 Introduction (F. Medina-Bolivar)
3:35 P-26 Unraveling the Catalytic Specificity of Terpene Biosynthetic Enzymes and Engineering the Biosynthesis of Novel Terpenes in Yeast and Plants
Joe Chappell, University of Kentucky
3:50 P-27 The Role of Ethnomedical Knowledge in Defining Methods for Large-scale In Vitro Cultivation: Study Cases of Two Mexican Medicinal Plants
María Luisa Villarreal, Universidad Autónoma del Estado de Morelos
4:25 P-28 Sub-lethal Levels of Electric Current Elicit the Biosynthesis of Plant Secondary Metabolites
Evans Kaimoyo, University of Arizona
4:25 P-29 Engineering Ascorbate for Enhanced Growth, Nutritional Content, and Stress Tolerance in Crops
Argelia Lorence, Arkansas State University

 

STEM CELLS IN CANCER RESEARCH AND THERAPY

Conveners:        Eugene Elmore, University of California – Irvine and Monika Schmelz, University of Arizona Health Sciences Center

3:30 pm – 5:00 pm Animal Symposium Coronado I

One hypothesis of cancer suggests that it arises from “cancer stem cells” that have the ability, similar to all stem cells, to self-renew and to differentiate into multiple cell types. Cancer stem cells could persist in cancers and would be the likely cause of the tumor resistance and relapse. The cancer stem cell theory is one of many explanations have been offered for the resistance of various cancers to therapy. Cancer stem cells that survive therapy are potentially able to give rise to new cancers.   Cells from cancer tissues are generally characterized by their rapid growth rates compared to cells from normal tissues, while stem cells tend to have much slower growth rates in vivo and would be more resistant to therapies that depend upon cell division for efficacy. Cancer stem cells would therefore survive therapy at doses that would kill most of the cells in a tumor, which would explain the resistance and potential for relapse. Progress in stem cell biology has permitted the isolation of stem cells from various embryonic and adult tissues, including cancer tissues. If cancer stem cell-specific biomarkers can be identified that are distinct from normal stem cell biomarkers, research could then target the differences in normal and cancer stem cell populations to identify specific therapies to target the cancer stem cells. This would allow the development of specific combination therapies to kill both the cancer and the cancer stem cells. This could ultimately result in better cancer survival rates and better quality of life for cancer patients. The speakers in this session will address the cancer stem cell issue and provide a critical update on the progress towards understanding the biology and future applications.

3:30 Introduction (E. Elmore and M. Schmelz)
3:35 A-15 Stem Cells: Ancestors in a Somatic Cell Tree
Darryl K. Shibata, University of Southern California Keck School of Medicine
4:00 A-16 Mechanism of Self-renewal of Brain Tumor Stem Cells
Ichiro Nakano, UCLA School of Medicine
4:25 A-17 Prostate Tissue Homeostasis
Monika Schmelz, University of Arizona Health Sciences Center
4:50 Discussion
5:00 pm – 5:30 pm SIVB Business Meeting
(All Members Are Urged to Attend)Student Award Presentations
Presidio V
5:45 pm – 10:00 pm An Evening at Old Tucson Studios
Admittance by Advance Ticket Holders Only
Old Tucson Studios
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