TUESDAY, JUNE 9
| 7:00 am – 5:00 pm | Registration | Presidio Registration Area |
LARGE SCALE, CELL AND TISSUE-BASED PRODUCTION OF BIOPHARMACEUTICALS AND SECONDARY METABOLITES
Conveners: Haruhiko Tsumura, Kirin Pharma Co., and Pamela J. Weathers, Arkansas Bioscience Institute
| 8:00 am – 10:00 am | Plenary Symposium | Presidio I and II |
This session will cover relevant aspects of production of biopharmaceutical and secondary metabolites by animal cells and plant cells and tissues in large scale. The development of production cell lines, culture medium, metabolic engineering, the use of genomic tools, bioreactor design and engineering, and some case studies of actual commercial production will be addressed. Where appropriate, speakers will address issues of economics. Attendees will benefit by learning about progress made in novel approaches to making bioproducts less expensive through innovation and how the correct decisions in large scale culture can improve downstream processing as illustrated by some success stories, along with how to avoid some of the pitfalls.
| 8:00 | Introduction (H. Tsumura and P. J. Weathers) | |
| 8:05 | PS-7 | Animal and Plant Cultures: Production of Biopharmaceuticals and Secondary Metabolites Wayne Curtis, Pennsylvania State University |
| 8:35 | PS-8 | Process Development for mAb Therapeutic Production in 10,000 L-reactors with CHO Cells Chikashi Hirashima, Chugai Pharmaceutical |
| 9:00 | PS-9 | Air Lift Balloon Type Bioreactor: Platform for Commercial Production of Plant Based Small Molecules and Tissues Ganapathy Sivakumar, Arkansas State University |
| 9:20 | PS-10 | Novel Plant Reactors for Pharmaceuticals Production Chunzhao Liu, Chinese Academy of Sciences |
| 10:00 am – 10:30 am | Coffee Break | Turquoise Ballroom |
HIGHLIGHT OF PLANT GENOMICS RESEARCH AT THE UNIVERSITY OF ARIZONA
Convener: Wayne Parrott, University of Georgia
| 10:30 am – 12:30 pm | Plant Symposium | Presidio III and IV |
The term genomics was adopted in the late 1980’s to encompass all aspects of mapping, sequencing and analysis of information of an organism’s entire genome. Today, the field also includes the characterization of genes, mRNAs and protein products (functional genomics), the study of DNA and histone modification patterns (epigenomics) and the elucidation of evolutionary relationships between genomes of different species (comparative genomics). Scientists at the University of Arizona had greatly contributed to the advancement of all aspects of plant genomics research. This session will highlight pioneer work unraveling the molecular basis of paramutation, the use of RNAi as a functional genomics tool and the study of evolution in plants using comparative genomics.
| 10:30 | Introduction (L. B. Jacobsen and E. J. Roemer) | |
| 10:35 | P-23 | Interchromosomal Transfer of Epigenetic Information Vicki L. Chandler, University of Arizona |
| 11:10 | P-24 | The iPlant Collaborative: A Cyberinfrastructure-Centered Community for a New Plant Biology Richard A. Jorgensen, University of Arizona |
| 11:45 | P-25 | The Oryza Map Alignment Project: Genomes in Flux Rod A. Wing, Arizona Genomics Institute |
| 12:20 | Discussion |
STEM CELLS, PLASTICITY, AND REGENERATIVE MEDICINE
Conveners: Paul J. Price, D-Finitive Cell Technologies, and Raziel S. Hakim, Howard University
| 10:30 am – 12:30 pm | Animal Symposium | Coronado I |
Stem cells have long been recognized as the source of mature differentiated cells in embryonic as well as mature organ systems. While the terms totipotent, pleuripotent and multipotent have been used to describe stem cells by the number of different mature cell types they can become, evidence is accumulating that the culture environment in which cells are held can not only affect the range of cell types that stem cells can become, but can even cause reprogramming of mature cells. The term transdifferentiation refers to mature cells which have been reprogrammed by environmental factors to new fates. Current research indicates that stem and even mature cells of post-natal origin can play a key role in cell-based therapies. The 3 speakers will discuss their research results with different sources of post-natal stem cells.
| 10:30 | Introduction (P. J. Price and R. S. Hakim) | |
| 10:35 | A-12 | Mesenchymal Stem Cells and the Development of Therapeutics Michelle Greene, Millipore Corporation |
| 11:10 | A-13 | Mesenchymal Progenitors Able to Differentiate into Osteogenic, Chondrogenic, and/or Adipogenic Cells In Vitro are Present in Most Primary Fibroblast-like Cell Populations Kazuhiro Sudo, Riken Bio Resource Center |
| 11:45 | A-14 | Induction of Hepatocyte-like Cells from Mesenchymal Stem Cells and the Transplantation into Liver-injured Rats Kiyohito Yagi, Osaka University |
| 12:20 | Discussion |
Tuesday, June 17
Odd Poster Authors will be present
1:30 pm – 2:30 pm
ANIMAL CELL BIOTECHNOLOGY
Moderator: Kim O’Connor, Tulane University
| 1:30 pm – 3:30 pm | Animal Contributed Paper Session | Coronado I |
| 1:30 | A-1006 | Multiendpoint Mechanistic Profiling of Hepatotoxicants in HepG2/C3A Human Hepatoma Cells and Comparison of Statistical Methods for Development of a Prediction Model for Acute Hepatotoxicity Thomas J. Flynn, US FDA, and Martine S. Ferguson |
| 1:50 | A-1007 | In Vitro Cellular Response to Nanoparticle Exposure Julie Elaine Morgan, Clayton State University, and J. A. Jordan |
| 2:10 | A-1008 | Computational Tissue Engineering: Monte-Carlo Simulation of Restructuring Dynamics During Tissue Self-assembly of Prostate Cancer Spheroids Kim C. O’Connor, Tulane University, and H. Song |
| 2:30 | A-1009 | Tools for Genetic Characterization and Identification of Cell Lines Manohar Rajeev Furtado, Applied Biosystems, R. Fang, J. G. Shewale, and F. Hyland |
| 2:50 | A-1010 | Mapping Signaling Pathways That Control Gap Junction Function Using Modern Proteomic Approaches Brad Luther Upham, Michigan State University, D. A. Whitten, C. G. Wiklerson, J. S. Park, I. Sovadinova, P. Babica, J. E. Trosko, and L. Blaha |
| 3:10 | A-1011 | Online Monitoring of Physiological Parameters of Cell Cultures Ralf Ehret, Bionas GmbH, Elke Thedinga, Sabine Drechsler, Axel Kob, Marcus Wego, Sebastian Rost, Steffen Fürst, and Werner Baumann |
STEM CELL BIOLOGY AND CANCER DRUG DEVELOPMENT
Moderator: Lia H. Campbell, Cell and Tissue Systems, Inc.
| 2:30 pm – 3:30 pm | Animal Interactive Poster Session | Turquoise Ballroom |
| A-2008 | Retaining Cell Integrity During Organotypic Model Viability Assays: Alternatives to MTT Catherine R. Kavanagh, State University of New York-Stony Brook, L. J. Crawford, K. M. Sawicka, S. R. Simon, and E. J. Roemer |
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| A-2009 | In Vitro Investigation of Antioxidant and Antiproteolytic Properties of the Clove Extracts: Tellimagrandin I and Casuarictin Sumaira Zamurrad, State University of New York-Stony Brook, S. Parrino, F. Daccueil, E. J. Roemer, and S. R. Simon |
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| A-2010 | Optimization of a New Method of Characterizing Live Basal Keratinocytes Using Chariot Transfection Reagent Yusuke Fukuda, State University of New York-Stony Brook, E. J. Roemer, S. R. Simon, and M. Matic |
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| A-2011 | Comparison of Growth Characteristics, Neurochemical Parameters and Response to Toxicants for Neural Tissue Derived Cell Lines from Goldfish and from Crayfish Lucila E. J. Lee, Wilfrid Laurier University, M. R. Bufalino, and M. P. Wilkie |
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| A-2012 | Assessing the In Vitro Respiratory Toxicity of Fine Particles of Al2O3 and SiO2: a Precursor Study for Lunar Dust Toxicity Jacqueline A. Jordan, Clayton State University, A. M. Verhoff, and D. G. Fischer |
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| A-2013 | D-Glucose Protection Against MPP+ Induced Cell Death in Human Lung Carcinoma A549 Cell Line David Fouad Elmashat, Florida A&M University, Ramesh B. Badisa, and Karam F. Soliman |
PLANT TRANSFORMATION, RNAi, AND PHARMACEUTICAL/VALUE ADDED TRAITS
Moderator: Sandra L. Kelly, Agriculture and Agri-FoodCanada
| 2:30 pm – 3:30 pm | Plant Interactive Poster Session | Turquoise Ballroom |
| P-2006 | Phloem Specific Transgene Expression Driven by AtSUC2 Gene Promoter in Transgenic Citrus Plants to Develop Citrus Greening Resistance Ahmad Al-Sayed Omar, University of Florida, Manjul Dutt, Gary Barthe, Vladimir Orbovic, and Jude Grosser |
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| P-2007 | Genetic Manipulation for Enhancing Calcium Uptake in Lettuce Sung Hun Park, Kansas State University, M. P Elless, J. Park, W. Lim, and K. D. Hirschi |
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| P-2008 | Optimization of Transformation Efficiency in Flax Sandra L. Kelly, Agriculture & Agri-Food Canada, and Mark C. Jordan |
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| P-2009 | Base-by-Base Analysis of siRNA Production by a Plant Transgene Victor Gaba, ARO Volcani Center, Y. M. Shiboleth, S. Singer, E. Kukurt, D. Liebmann, L. Maslenin, A. Rosner, and A. Gal-On |
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| P-2010 | Wheat Virus Resistance Via Interference RNA Luisa F. Cruz, Kansas State University, John P. Fellers, and Harold N. Trick |
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| P-2011 | Partial Characterization and Purification of Plant Derived Butyrylcholinesterase to Treat Organophosphate Poisoning Latha Kannan, Arizona State University B. C. Geyer, P.-E. Garnaud, R. R. Woods, M. Muralidharan, I Cherni, and T. S. Mor |
IN VITRO TOOLS, TECHNIQUES, AND OPTIMIZATION
Moderator: Michael E. Kane, University of Florida
| 3:30 pm – 5:00 pm | Plant Contributed Paper Session | Presidio I and II |
| 3:30 | P-1006 | Seed Physiology of Bletia purpurea (Pine Pink; Orchidaceae)—Fluctuating Low Temperature and Dark Slow Development and Inhibit Germination Timothy R. Johnson, University of Florida, M. E. Kane, and H. E. Perez |
| 3:45 | P-1007 | Asymbiotic Seed Germination and In Vitro Seedling Development of Cyrtopodium punctatum: A Propagation Protocol for an Endangered Florida Native Orchid Daniela Dutra, University of Florida, M. E. Kane, and L. Richardson |
| 4:00 | P-1008 | Synergistic Effect of Auxin and Cytokinin on In Vitro Androgenesis in Azadirachta indica A. Juss Rakhi Chaturvedi, Indian Institute of Technology-Guwahati, and Priyanka Srivastava |
| 4:15 | P-1009 | Standardization of Protocol for Efficient In Vitro Clonal Propagation of Rare Medicinal Plants e.g., Elaeocarpus & Capparis L. N. Shukla, B. R. Ambedkar Bihar University, C. P. Shukla, B. K. Mishra, Manoj Kumar, Sushma Kumari, and T. Upadhyay |
| 4:30 | P-1010 | Germplasm Evaluation of Andographis paniculata (Kalmegh) Through Chemoprofiling for In Vitro Mass Multiplication of Quality Germplasm from Satpura Plateau Region of Madhya Pradesh Shailendra Kumar Tiwari, State Forest Research Institute, Vijay Bahadur, Amit Pandey, Shweta Mishra, M. P. Goswani, and Pankaj Bhargava |
| 4:15 | P-1011 | Micropropagation of Boerhaavia diffusa – A Valuable Medicinal Plant Regha P. Periyannan, Muthyammal College of Arts and Sciences, N. Vinod Kumar, M. S. Kavitha, M. Rajasekara Pandian, and E. G. Wesely |
NEW STRATEGIES FOR PRODUCTION OF SPECIALIZED METABOLITES
Convener: Fabricio Medina Bolivar, ABI/Arkansas State University
| 3:30 pm – 5:00 pm | Plant Symposium | Presidio III and IV |
Plants have evolved specialized networks for the biosynthesis of a rich repertoire of natural products. These complex molecules serve as chemical languages in ecosystems, and often confer protective characteristics to plants allowing them to survive, and prosper in a multitude of challenging ecological niches. Specialized metabolism is an economically important source of fine chemicals, such as medicines, insecticides, dyes, flavors, and fragrances. Scientists have made significant progress at developing strategies to study and alter specialized metabolism. In particular, genomics and metabolomics approaches are leading to an advanced understanding of how these metabolic networks function in a coordinated fashion leading to desired traits or phenotypes. This symposium will provide an update on novel strategies used by multidisciplinary teams to harness and alter biosynthetic pathways for the production of natural products with diverse bioactivities.
| 3:30 | Introduction (F. Medina-Bolivar) | |
| 3:35 | P-26 | Unraveling the Catalytic Specificity of Terpene Biosynthetic Enzymes and Engineering the Biosynthesis of Novel Terpenes in Yeast and Plants Joe Chappell, University of Kentucky |
| 3:50 | P-27 | The Role of Ethnomedical Knowledge in Defining Methods for Large-scale In Vitro Cultivation: Study Cases of Two Mexican Medicinal Plants María Luisa Villarreal, Universidad Autónoma del Estado de Morelos |
| 4:25 | P-28 | Sub-lethal Levels of Electric Current Elicit the Biosynthesis of Plant Secondary Metabolites Evans Kaimoyo, University of Arizona |
| 4:25 | P-29 | Engineering Ascorbate for Enhanced Growth, Nutritional Content, and Stress Tolerance in Crops Argelia Lorence, Arkansas State University |
STEM CELLS IN CANCER RESEARCH AND THERAPY
Conveners: Eugene Elmore, University of California – Irvine and Monika Schmelz, University of Arizona Health Sciences Center
| 3:30 pm – 5:00 pm | Animal Symposium | Coronado I |
One hypothesis of cancer suggests that it arises from “cancer stem cells” that have the ability, similar to all stem cells, to self-renew and to differentiate into multiple cell types. Cancer stem cells could persist in cancers and would be the likely cause of the tumor resistance and relapse. The cancer stem cell theory is one of many explanations have been offered for the resistance of various cancers to therapy. Cancer stem cells that survive therapy are potentially able to give rise to new cancers. Cells from cancer tissues are generally characterized by their rapid growth rates compared to cells from normal tissues, while stem cells tend to have much slower growth rates in vivo and would be more resistant to therapies that depend upon cell division for efficacy. Cancer stem cells would therefore survive therapy at doses that would kill most of the cells in a tumor, which would explain the resistance and potential for relapse. Progress in stem cell biology has permitted the isolation of stem cells from various embryonic and adult tissues, including cancer tissues. If cancer stem cell-specific biomarkers can be identified that are distinct from normal stem cell biomarkers, research could then target the differences in normal and cancer stem cell populations to identify specific therapies to target the cancer stem cells. This would allow the development of specific combination therapies to kill both the cancer and the cancer stem cells. This could ultimately result in better cancer survival rates and better quality of life for cancer patients. The speakers in this session will address the cancer stem cell issue and provide a critical update on the progress towards understanding the biology and future applications.
| 3:30 | Introduction (E. Elmore and M. Schmelz) | |
| 3:35 | A-15 | Stem Cells: Ancestors in a Somatic Cell Tree Darryl K. Shibata, University of Southern California Keck School of Medicine |
| 4:00 | A-16 | Mechanism of Self-renewal of Brain Tumor Stem Cells Ichiro Nakano, UCLA School of Medicine |
| 4:25 | A-17 | Prostate Tissue Homeostasis Monika Schmelz, University of Arizona Health Sciences Center |
| 4:50 | Discussion |
| 5:00 pm – 5:30 pm | SIVB Business Meeting (All Members Are Urged to Attend)Student Award Presentations |
Presidio V |
| 5:45 pm – 10:00 pm | An Evening at Old Tucson Studios Admittance by Advance Ticket Holders Only |
Old Tucson Studios |