2008_Tuscon

Daily Program
Abstracts & Posters
Saturday, June 14 Keynote Symposium Plant Contributed Papers
Sunday, June 15 Plenary Symposia Animal Posters
Monday, June 16 Animal Symposia Plant Posters
Tuesday, June 17 Plant Symposia Education Posters
Wednesday, June 18 Invertebrate Conference Symposia 2008 Abstract Index
Education Symposia Late Submission Abstracts
Animal Contributed Papers

MONDAY, JUNE 16

7:00 am – 6:00 pm Registration Presidio Registration Area

BIOINFORMATICS, GENOMICS, PROTEOMICS AND CELLOMICS

Conveners:        Colette J. Rudd, XenoPort, Inc., and Mark C. Jordan, Agriculture and Agri-Food Canada

8:00 am – 10:00 am Plenary Symposium Presidio I and II

New technologies with the potential to rapidly increase our understanding of the complexities within biological systems are becoming available. These include next generation sequencing technologies and advances in tools for rapid analysis of a broad profile of cellular constituents. As these technologies come into widespread use it will be essential for cell biologists to develop strategies to handle, analyze and integrate large quantities of data.

8:00 Introduction (C. J. Rudd and M. C. Jordan)
8:05 PS-4 Next Generation Sequencing Technologies, Their Implications, and Prospects for Next-Next Gen Technologies
Jeffrey Schloss, National Human Genome Research Institute –National Institutes of Health
8:40 PS-5 Peptidomic Profiling of Endocrine Cell Culture Media for Bioactive Peptide Discovery
Steve Taylor, Amylin Pharmaceuticals
9:15 PS-6 Data Management and Extraction of Biological Information from Large Data Sets
David Mount, University of Arizona
9:50 Discussion

 

10:00 am – 10:30 am Coffee Break Turquoise Ballroom

 

BIODIVERSITY FOR IMPROVING HUMAN HEALTH

Convener:         Argelia Lorence, ABI/Arkansas State University

10:30 am – 12:30 pm Plant Symposium Presidio I and I

Nature is regarded as a main source of remedies throughout history.  Nowadays many cultures still rely on the large diversity, particularly of plants, as a source of medicines.  Importantly, more than 50% of small-molecule drugs introduced in the last years have been inspired or derived from a natural source. The advances in cell and tissue culture techniques applied to this rich biodiversity offer possibilities for the production and discovery of novel bioactive natural products with importance on human health.  In this session we will combine presentations that explore nature’s biodiversity, plant cell/tissue culture and novel in vivo biological assays for the production of natural products with applications as medicines and nutraceuticals.

10:30 Introduction (A. Lorence)
10:35 P-11 Searching for New Antiviral Agents from Brazilian Biodiversity
Claudia Maria Oliveira Simões, CCS Universidade Federal de Santa Catarina
11:00 P-12 Improving Cat’s Claw Alkaloid Production by Stimulating In Vitro Plant Cultures
Ana Ramos-Valdivia, CINVESTAV
11:25 P-13 Production and Neuroprotective Properties of Natural Resveratrol Analogs from Hairy Root Cultures of Peanut
Fabricio Medina-Bolivar, Arkansas State University
11:50 P-14 The Use of the Worm Caenorhabditis elegans as a Model to Investigate Functional Ingredients
Daniel Ramón Vidal, BIOpolis
12:15 Discussion

 

GLYCO-ENGINEERING OF PHARMA PROTEINS FROM PLANT CELLS

Conveners:        Michael E. Horn, Targeted Growth, Inc., and Vincent P. Wingate, Biolex

10:30 am – 12:30 pm Plant Symposium Presidio III and IV

Most human therapeutic proteins are glycoproteins and this includes all of the monoclonal antibodies. Proper N-glycosylation is important for their proper folding of the protein, in vivo stability and biological activity in the patient. There are major similarities and important differences between the N-glycosylation pattern of plant and mammalian N-glycosylated proteins. The differences exist mainly in the late Golgi functions. For example, plants attach an a1,3 linked fucose residue to the middle of the chain while mammals attach an a1,6 fucose. Another example is that plants also attach a b1,2 xylose residue that is foreign in mammalian systems. These two differences have been implicated in immunogenicity studies. Some therapeutic proteins require a terminal sialic acid for complete functionality and plant cells do not generally synthesize this residue. The presentations in this session will cover a broad range of methods being successfully employed to humanize the N-glycosylation pattern of plant-made pharmaceutical proteins. These methods include the elimination or down-regulation of certain key endogenous enzymes as well as the addition of certain mammalian N-glycosylation enzymes.

10:30 Introduction (M. E. Horn and V. P. Wingate)
10:35 P-15 Humanization of N-glycosylation of Nicotiana benthamiana for Production of Biotherapeutics using MagnICON
Koen Weterings, Bayer BioScience NV
11:10 P-16 The Power of One: Glyco-optimized Therapeutic Antibodies in Lemna
John R. Gasdaska, Biolex
11:45 P-17 Sustainable Glyco-engineering and Production of Optimized Biopharmaceuticals in Bryophytes
Gilbert Gorr, Greenovation Biotech GmbH
12:20 Discussion

 

GOOD CELL CULTURE LABORATORY PRACTICES

Conveners:        John W. Harbell, Mary Kay, Inc., and Tetsuji Okamoto, HiroshimaUniversity

10:30 am – 12:30 pm Animal Symposium Coronado I

Cell and tissue-based bioassays have been a mainstay for drug development, cancer research and basic cell physiology research for several decades. With the growth of molecular biology, genomics, and predictive in vitro toxicology, in vitro systems are being used by researchers of diverse backgrounds. Some researchers may see the cell cultures as simply another reagent. The study of cells in isolation provides many advantages and also considerable challenge. Often cited advantages include precise control over exposure conditions (concentration and duration), greater freedom in selection of the test species, use of well characterized cell types, and the ability to handle a large number of samples at a reasonable cost. The potential advantages can be realized only with a full understanding of the challenges. The initial characterization of the test system and maintenance of its homeostasis and consistency must be designed into the assay. The endpoints of the assay are necessarily indirect and often focus on the early cellular changes that precede the macroscopic changes in the whole organ or organism. This symposium will focus on assuring the validity of the test system as free from occult contamination and consisting of the cell type expected based on experience from several perspectives. In addition, it will address experimental design and the training required for proper execution of the studies.

10:30 Introduction (M. E. Horn and V. P. Wingate)
10:35 A-7 Mycoplasma Contamination and Cross Contamination in Tissue Culture: A Survey of Major Institutions in Japan
Arihiro Kohara, National Institute of Biomedical Innovation
11:10 A-8 Assuring Cell-based Assay Quality by Design and Execution: A Contract Research Laboratory Perspective
Hans A. Raabe, Institute for In Vitro Sciences
11:45 A-9 Homogenous Cell Cultures: Understanding Cross Contamination and Maintaining Culture Integrity
Amy Wright, Ciba Vision Corporation
12:20 Discussion

 

STUDENT NETWORKING LUNCHEON

Conveners:        Nancy Jean Engelmann, University of Illinois-Urbana/Champaign, and Phillip J. Kauth, University of Florida

12:30 pm – 2:00 pm Education Symposium Presidio V

Young scientists and their advisors are invited to attend this luncheon to discuss what they learned from Saturday and Sunday’s workshops.  This will also be an excellent opportunity for attendees to further network with the speakers and mock interviewers from Sunday night.  A final goal is to determine what other areas of career development are necessary to the young SIVB scientist and could be focused on for the 2009 Student Committee hosted session.

12:30 Introduction (N. E. Engelmann and P. J. Kauth)
12:40 E-7 Student Networking
David D. Songstad, Monsanto Company

 

Monday, June 16
Even Poster Authors will be present
1:30 pm – 2:30 pm

 

STEM CELL BIOLOGY AND CANCER DRUG DEVELOPMENT

Moderator:         Kim O’Connor, Tulane University

1:30 pm – 3:30 pm Animal Contributed Paper Session Coronado I
 1:30  A-1000 Identification of a Homogeneous Adult Stem Cell Population, miR Signatures, and miR-Dependent Differentiation
Frederick O. Cope, Ohio State University, and M. S. Blue
 1:50  A-1001 Expansion of Pancreatic Stem Cells from Human Islet of Langerhans Preparations
Lia H. Campbell
, Cell and Tissue Systems, Inc., Nancy L. Parenteau, and Kelvin G. M. Brockbank
 2:10  A-1002 Growth Factors and Extracellular Matrix Components Induce Formation of Membranes in Mouse Embryonic Stem Cells
A. R. Calabro, St. John’s University, Frank A. Barile
 2:30  A-1003 Melain Synthersis Inhibiting Activity of MC20 Isolated from a Marine Red Alga, Calliiarthron yessoense in B16 Melanoma Cells
Yuto Kamei, Saga University, and Y. Ohtsuka
 2:50  A-1004 Responses of Pineapple Stem Bromelain in a Human Cell Screening Assay for Melanoma Prevention
Eugene Elmore, University of California-Irvine, Aarti Jain, Vernon E. Steele, and J. Leslie Redpath
 3:10  A-1005 Anti-cancer Effect of Enzyme-digested Fucoidan Extract from Seaweed Mozuku
Sanetaka Shirahata, Kyushu University, Kiichiro Teruya, Sakiko Matsuda, Ayumi Nakano, Takuya Nishimoto, Masashi Ueno, Kenji Shirouzu, Makiko Yamashita, Hiroshi Eto, and Yoshinori Katakura

 

IN VITRO ANIMAL CELL SCIENCES INTERACTIVE POSTERS

Moderator:         Lia H. Campbell, Cell and Tissue Systems, Inc.

2:30 pm – 3:30 pm Animal Interactive Poster Session Turquoise Ballroom
 A-2000 Prostaglandin A2 Significantly Alters Gene Expression in an Established Insect Cell Line (BCIRL-HzAM1)
Cynthia L. Goodman, USDA/ARS/BCIRL, D. Stanley, Q. Song, S. An, and A. McIntosh
 A-2001 Granulocytic Differentiation of HL-60 Promyelocytic Leukemia Cells is Associated with Increased mRNA Expression for Components of the Cullin-5 Containing E3 Ubiquitin Ligase
Shaneen S. Baxter, Midwestern University, Lauren A. Carlson, Alejandro M. S. Mayer, Mary L. Hall, and Michael J. Fay
 A-2002  Organ and Monolayer Cell Culture of Gottingen Minipig Skin: A Model for Whole Skin Study and Drug Safety Screening
Michael K. Dame, University of Michigan Medical School, and Diana Spahlinger, Marissa DaSilva, Patricia Perone, Robert Dunstan, and James Varani
 A-2003 Effects of Rheum ribes Ethylacetate Extracts on Cytochromes P450 1B1 Gene Expression and Glutathione-S-transferase Activity in HL-60 cells
Pembegul Uyar, Middle East Technical University
 A-2004 In Vitro Investigation of Individual and Combined Cytotoxicity of Ochratoxin A and Arsenic in Chinese Hamster Lung Fibroblasts and Human Urothelial Cells
Manoj Aggarwal, Indian Veterinary Research Institute, C. Behm, W. Foellmann, J. K. Malik, and G. H. Degen
 A-2005 In Vitro Evaluation of Nano-encapsulated Protective Antigen Functionality Through the Use of a Monomac 6 Assay
Kevin E. Knockenhauer, State University of New York-Stony Brook, Katarzyna M. Sawicka, Elizabeth J. Roemer, and Sanford R. Simon
 A-2006 Update on the COLIPA Eye Irritation Programme for Development of In Vitro Methods
Penny A. Jones, Unilever, Sandrine Bessou-Touya, Lieve Declercq, Ann De Smedt, Bart De Wever, Claudine Faller, John Harbell, Béatrice Le Varlet, Pauline McNamee, Monique Marrec-Fairley, Wolfgang Pape, Uwe Pfannenbecker, Klaus Schroeder, Magalie Tailhardat, Christine Van den Berghe, and Freddy Van Goethem

 

PLANT TISSUE CULTURE, MICROPROPAGATION AND SECONDARY METABOLITES

Moderator:         Carol Potenza, USDA/ARS

2:30 pm – 3:30 pm Plant Interactive Poster Session Turquoise Ballroom
 P-2000 Secondary Metabolism of Hypericum perforatum Induced by Agrobacterium rhizogenes
Eliane R. Santarem, Pontificia Universidade Catolica do Rio Grande do Sul, D. C. Zamban, L. M. Felix, and L. V. Astarita
 P-2001 Establishment and Rooting of In Vitro Micro-cuttings from Winter Buds of Quercus kelloggii
D. Kitterman, California State University, J. Johnson, and J. Bushoven
 P-2002 Aseptic Coculture of Native Plant Derived Calli with Native and Nonnative Seedlings Affects Growth in a Dose Dependent and Contact Independent Manner
Carol Potenza, USDA/ARS, K. Yeater, D. James, and J. Barrow
 P-2003 Iridoids Accumulation in Root Cultures of the Cancer Herb Castilleja tenuiflora
Gabriela Trejo-Tapia, Centro de Desarrollo de Productos Bióticos-IPN, G. Rosas, A. Zamilpa, K. Bermudez, and M. Rodríguez
 P-2004 Growth Patterns, Secreted Protein Profiles, and EST Transcripts from Sugar Beet (Beta vulgaris L.) Hairy-Root Cultures
Brett J. Savary, Arkansas State University, Prasanna Vasu, Daya Anandan, Ann C. Smigocki, and Alberto Nuñez
 P-2005 In Vitro Studies of Tropical Woody Species
Somika Bhatnagar, Temasek LifeSciences Laboratory, S. Chandrasekharan, D.Y. Xie, and Y. Hong

 

ISSUES IN FIELD RELEASE OF TRANSGENIC PLANTS BY ACADEMIC INSTITUTIONS

Convener:          Tom Currier, Bayer CropScience

3:30 pm – 5:00 pm Plant Symposium Presidio III and IV

According to the “Recommendations for Management Practices for Field Trials with Bioengineered Plants” prepared by the National Agricultural Biotechnology Council*,  “application, institutional responsibility, approvals, training, fieldsite selection, record-keeping, communications, storage and disposal of biological materials, appropriate treatment of equipment including cleaning, monitoring, testing, and reporting are processes common to all research on transgenic plants”. In order to test new transgenic plants in real conditions such as small-scale field studies, investigators at Universities and other institutions have to comply with federal and local regulatory requirements.  These regulations apply to all events not yet approved by the government agencies for unlimited commercial field release.  They require submission of information about the material to be planted, and extensive follow-up documentation of the field trials themselves.   A number of guidance documents have been developed by institutions to help investigators meet these needs. Speakers at this session will describe their experiences in dealing with these requirements in the USA, and discuss how institutions can best develop their management practices to meet the requirements.  A round-table discussion of their experiences and those of other symposium participants will be held at the end of the session.

*http://nabc.cals.cornell.edu/Publications/WhitePapers/Recomm_final.pdf

3:30 Introduction (L. H. Campbell and L. B. Jacobsen)
3:35 P-18 Regulating Transgenic Plants for Academic Research
C. Neal Stewart, Jr., University of Tennessee
4:00 P-19 Field Evaluation of Regulated Transgenic Plants in an Academic Environment
Thomas E. Clemente, University of Nebraska-Lincoln
4:25 P-20 Controlled Field Release of Pharmaceutical Corn in Iowa: Lessons and Strategies
Kan Wang, Iowa State University
4:50 Discussion

 

LEONARD J. SCHIFF MEMORIAL SYMPOSIUM:

CURRENT STATUS OF TISSUE-ENGINEERED PRODUCT REGULATION:

A GLOBAL VIEW ON THE RELATIONSHIP OF SCIENCE AND PRACTICALITY

Conveners:        Sandra L. Schneider, Research & Clinical Laboratory Systems, and Tohru Masui, JCRB Cell Bank

3:30 pm – 5:00 pm Animal Symposium Coronado I

Development of tissue-engineered cells, cellular products and therapeutic biologics requires an understanding of current regulatory issues. Numerous mandate and guideline changes have occurred in United States (U.S.), European and Japanese law governing clinical studies. As in vitro scientists, it is important to understand the key regulatory issues when conducting global pre-clinical and biologic research that have public interest and response. The symposium objective is to build on previous Congress presentations related to regulatory pathways in discovery and development of biopharmaceuticals and cellular therapies. This includes research design and other practical considerations associated with bench to bedside translational biologics. The symposium outcome is to gain a global understanding of the essential US, European and Japanese regulatory process related to cellular products and transplantation of cultured cells.

3:30 Introduction (S. L. Schneider and T. Masui)
3:35 A-10 The Regulation of Cultured Cells and Cellular Products for Transplantation: Current View of the Japanese Regulatory Process
Tohru Masui, JCRB Cell Bank
4:00 A-11 Navigating Research Strategy, Clinical Integrity and Current Global Regulatory Compliance
Sandra L. Schneider, Research & Clinical Laboratory Systems
4:10 Roundtable/Panel Discussion
John W. Harbell, Mary Kay Inc.
Tohru Masui, JCRB Cell Bank
Quan Nguyen, Nguyen & Tarbet
Colette Rudd, Xenoport, Inc.

 

PLANT VACCINES, PHARMACEUTICALS, AND ALLERGEN REDUCTION

Moderator:         Mohammed Kamal Chowdhury, ClaflinUniversity

3:45 pm – 5:00 pm Plant Contributed Paper Session Presidio I and II
 3:45  P-1001 Transformation of Tomato with Anti-malarial Genes with an Aim to Produce Edible Vaccines
Mohammed Kamal Chowdhury, Claflin University, and Mihail Kantor
 4:00  P-1002 In Planta Expression and Molecular Characterization of the Candidate HIV-1 Mucosal Vaccine CTB-MPR649-684
Nobuyuki Matoba, Arizona State University, H. Kajiura, I. Cherni, J. D. Doran, M. Bomsel, K. Fujiyama, and T. S. Mor
 4:15  P-1003 Production of Cervical Cancer-related HPV 16E7 as a Pharmaceutical Protein in Rice Seeds
Amit Mehra, University of Arkansas, and Brad J. Murphy
 4:30  P-1004 Plant-derived Intimin Vaccine to Prevent Colonization of Enterohaemorragic Escherichia coli
Emel Topal, Biodesign Institute at Arizona State University, Maria Lucrecia Alvarez, and Hugh S. Mason
 4:45  P-1005 Elimination of the Three Major Allergens in Transgenic Peanut (Arachis hypogea L)
Anthony Okello Ananga, Alabama A&M University, Hortense Dodo, and Koffi Konan

 

EFFECT OF MEDIUM CONSTITUENTS ON CELLS

Convener:           Paul J. Price, D-Finitive Cell Technologies

5:00 pm – 6:00 pm Animal Workshop Coronado I

Eukaryotic cell culture media contain a diverse mixture of nutrient constituents, particularly formulations designed for serum-free culture. Optimization of nutrient additives to cultivate specific cell types or to produce high yields of biological product requires consideration beyond intermediary metabolism of the independent nutrients. This workshop explores interdependencies of nutrient constituents and external factors that impact stability or efficacy.

5:00 Introduction (P. J. Price)
5:05 Hydrolysates
Matt Caple, SAFC Biosciences
5:10 Nutrient Feeding
Tom Fletcher, Irvine Scientific
5:15 Factorial Design Made Easy
Steve Peppers, Invitrogen
5:20 Effect of Temperature and Light on Medium Constituents
Paul Price, D-Finitive Cell Technologies
5:25 Panel discussion
5:55 Concluding Remarks
Paul Price, D-Finitive Cell Technologies

 

EXPERIMENTAL DESIGN FOR IN VITRO BIOLOGISTS

Convener:           Randall P. Niedz, USDA /ARS

5:00 pm – 6:00 pm Plant Workshop Presidio III and IV

Workshop participants will discuss and learn some of the conceptual and practical aspects of how to plan and design multivariate experiments – as opposed to the mechanistic details of how to make certain types of statistical calculations.  Because experimental design is inherently geometric, the workshop will focus on how to think about the ‘geometry’ of experiments as defined by the independent factors, their ranges and the responses of interest to be measured.  The relative merits of multivariate versus one-factor-at-a-time (OFAT) approaches will be explored.  Primary discussion topics will include 1) how to conceptually design an experiment from a geometric perspective; 2) how to evaluate the “quality” of the resulting design; 3) how to evaluate the “quality” of measured data; and 4) how to select and utilize software applications to facilitate the conversion of the researcher’s subject matter expertise into statistically robust experimental designs.  Additionally, time will be allocated for discussion of specific research questions from participants

5:00 Introduction (R. P. Niedz)
5:05 Discussion
Terrance J. Evens, USDA/ARS
Randall P. Niedz, USDA/ARS

 

MICROSCOPY TOOLS FOR THE PLANT BIOTECHNOLOGIST

Convener:          Mary Welter, Dow AgroSciences

5:00 pm – 6:00 pm Plant Symposium Presidio V

The most fundamental processes associated with plant cell biology and gene expression are sub-cellular in nature. As such, microscopy plays a central role in enabling critical observations to be made. Live cell imaging, FISH, FRET and laser capture microdissection are but a few of the microscopy-based techniques currently used to describe sub-cellular phenomenon in plants. Although many of these techniques have become highly standardized and relatively simple to use, the risk of misinterpretation can be great if the methods used are not well understood and systematically implemented. This workshop will explore some of the potential applications and inherent limitations of several of these methods.

5:00 Introduction (M. Welter)
5:05 P-21 Imaging Live Plant Cells and Plant Cells
Sidney L. Shaw, Indiana University
5:25 P-22 Plant Microscopy: Perils and Premises
Elison B. Blancaflor, The Samuel Roberts Noble Foundation Inc.
5:45 Discussion
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