WEDNESDAY MAY 26
7:00 am – 5:00 pm | Registration | Market Street Foyer |
CELL MODELS/BIOTECHNOLOGY
Moderator: Lynne Rutzky, The University of Texas Medical School8:00 am – 10:00 am | Joint Vertebrate/Toxicology Contributed Paper Session | Seacliff C & D |
8:15 | VT-1007 |
Adult Mammalian Spinal Cord Neuron Culture in a Defined System
Mainak Das, Clemson University, Cassie Gregory, Neelima Bhargava, Peter Molnar, Lisa Riedel, and James J. Hickman |
8:30 | VT-1008 |
A Defined System for the Systematic Study of Cardiac Myocyte Development and Physiology – Applications for Biocompatibility and Toxin Detection
Peter Molnar, Anupama Natarajan, Anahita Jamshidi, Mainak Das, Cassie Gregory, ChangJu Chung, and James J. Hickman |
8:45 | VT-1009 | The Multisolution Hypothesis of Cell Preservation Robert G. Van Buskirk, Binghamton University, Aby J. Mathew, John M. Baust, Kristi Karin Snyder, and John G. Baust |
9:00 | VT-1010 | Hypothermic Storage of Neonatal Mammalian Cardiomyocytes: Assessment of Multiple Markers of Viability Kristi Karin Snyder, Binghamton University, John M. Baust, Robert G. Van Buskirk, and John G. Baust |
9:15 | VT-1011 | Modeled Microgravity Culture of Human Pancreatic Islets Improves Function and Reduces Apoptosis Lynne P. Rutzky¸The University of Texas Medical School, Szczepan Bilinski, Malgorzata Kloc, Huimin Zhang, Tammy Phan, Stephen M. Katz, and Stanislaw Stepkowski |
GENE DISCOVERY AND REGULATION
Moderator: Lining Tian, Agriculture and Agri-Food Canada
8:00 am – 10:00 am | Plant Contributed Paper Session | Grand Ballroom C |
8:00 | P-1008 |
Modern Techniques of Genetic Engineering: Agrobacterium-mediated Transformation of Creeping Bentgrass cv. PennA4 Using a Mutant BAS1 Construct for the Production of Dwarf Plants
J. Powell, University of Rhode Island, Chip Longo, Mike Budziszek, Alissa Neill, Joel Hague, Marina Ayrapetov, Joel Chandlee, Amy Gaudette, Brian Steere, Rachel Maxwell, Cathy Buckley, C. Smith, Jeremy Lins, Nicole Crothers, Alison Clark, Kristin Masso, Albert P. Kausch, and Michael Neff |
8:15 | P-1009 | Improving Forage Quality of Tall Fescue (Festuca Arundiacea) by Genetic Manipulation of Lignin Biosynthesis Zeng-Yu Wang, The Samuel Roberts Noble Foundation, Lei Chen, Chung-Kyoon Auh, Paul Dowling Andrew Hopkins, and Jeremey Bell |
8:30 | P-1010 | Transformation of Solanum phureja to Enhance Tuber Carotenoid Levels Steve Millam, Scottish Crop Research Institute, L. Ducreux, W. Morris, and M. Taylor |
8:45 | P-1011 |
Developing a Nicotiana benthamiana Network to Study Functional Genomics and Medicinal Plant Biology
Monto H. Kumagai, University of Hawaii |
9:00 | P-1012 | Repression of Gene Expression by HD2-type Histone Deacetylases and Function Analysis of HD2 Proteins in Arabidopsis Lining Tian, Agriculture and Agri-Food Canada, Keqiang Wu, Changhe Zhou, Helene Labbe, Sunandini Sridha, Li Wang, Marysia Latoszek-Green, Lin Zhang, Daniel C. Brown, and Brian L. Miki |
9:15 | P-1013 | A Barley LTP Promoter for Tissue-specific Expression of Transgene-mediated Disease Resistance Maria L. Federico, University of Wisconsin-Madison, R. W. Skadsen, and H. F. Kaeppler |
9:30 | P-1014 |
Ac/Ds Transposon-mediated Gene Discovery in Barley
Jaswinder Singh, University of California – Berkeley, Shibo Zhang, L. Cooper, S. Rangel, R. Kaur, A. K. Sturbaum, V. Edwards, Phil Bregitzer, P. M. Hayes, and Peggy G. Lemaux |
9:45 | P-1015 | Differential Gene Expression During Embryo Development in Maritime Pine (Pinus pinaster) Sonia C. M. Goncalves, J. Cairney, M. Oliveira, and C. Miguel |
MEDICINAL PLANT BIOTECHNOLOGY
Convener: Anand K. Yadav, Fort Valley State University, Marceline Egnin, Tuskegee University
8:00 am – 10:00 am | Plant Symposium | Grand Ballroom B |
The booming popularity of plant remedies actually began when the US Congress passed the Dietary Supplement Health and Education Act in 1994. World trade in different types of medicinal plant products has been steadily increasing owing to changing perceptions of prescription drugs and public preference for the botanical remedies without having side effects. The primary objective of this workshop is to assess the knowledge of current trends in biotechnology of medicinal and nutraceutical plants. Thus, this workshop provides an opportunity to exchange information on the current status of on-going biotechnology related research efforts on medicinal and nutraceutical plants. Experts working on different medicinal/nutraceutical plant species will emphasize their current specialty research work.
8:00 | Introduction | |
8:10 | P-30 | Environmental and Genetic Approaches to Increase Productivity of Medicinal Plants in Liquid Culture Jeffrey Adelberg, Clemson University |
8:30 | P-31 | In Vitro Regeneration and Greenhouse Establishment of Medicinal and Rare Skullcaps (Scutellaria) Nirmal Joshee, Fort Valley State University |
8:50 | P-32 | Tissue Culture Methods to Improve Herbs, Spices, and Nutraceutical Plants Alison M. R. Ferrie, Plant Biotechnology Institute/National Research Council Canada |
9:10 | P-33 | Developing Biotechnology to Regenerate and Improve Selected Nutraceutical Plants Bipul K. Biswas, Fort Valley State University |
9:30 | P-34 |
Prospects for Engineering Vaccinium Germplasm for the Production of a High-potency Resveratrol Analogue
Daniel C. Cook, USDA/ARS/NPURU |
VALIDATION AND REGULATORY ACCEPTANCE OF IN VITRO TOXICITY ASSAYS
Convener: John W. Harbell, Institute for In Vitro Sciences
8:00 am – 10:00 am | Animal Symposium | Seacliff A & B |
For the past four to five decades, the Tissue Culture Associations, worldwide, have sought to promote cell-based research and set standards for cell models, study design and publications. These efforts have succeeded to the point where cell culture methods have evolved from strictly academic tools to mainstays of many fields including biotechnology (plant and animal), drug discovery, and, increasingly, toxicology. This wider application often falls into the general category of bioassays where the cell culture systems are used prospectively to predict an action on the whole organ or organism. For many reasons, these in vitro methods are being applied to regulatory questions as well (e.g., drug efficacy, drug safety, environmental toxicology). Any bioassay is designed around four components; a specific cell or tissue, defined exposure conditions, well characterized endpoint measures of the biological effect, and a predetermined relationship between the in vitro endpoint values and the effect being predicted (e.g., potency, organ toxicity, etc.). The predictive capacity of the assay is dependent on maintaining the integrity and consistency of the whole system over time and often across laboratories. This symposium will examine the “good science” aspects of cell/tissue culture studies and how they contribute to the quality of the data generated. The topics will include Good Cell Culture Practices, study design optimization, and understanding the predictive capacity of tests. Special emphasis will be given to the design and validation of bioassays intended for use in the regulated environment.
8:00 | Introduction (J. W. Harbell) | |
8:15 | A-29 | Good Cell Culture Practices Guidelines Sandra Coecke, European Centre for the Validation of Alternative Methods |
8:45 | A-30 | In Vitro Bioassays in Toxicology: Focus on Design, Validation, and Regulatory Acceptance John W. Harbell, Institute for In Vitro Sciences |
9:15 | A-31 | Development and Application of In Vitro Assays to Predict Human Response Eugene Elmore, University of California – Irvine |
10:00 am – 10:30 am | Coffee Break | Market Street Foyer |
MICROSCOPY FOR THE 21ST CENTURY: NEW WAYS TO “SEE” INSIDE THE CELL
Convener: Alda Vidrich, University of Virginia Health Sciences Center
10:30 am – 12:30 pm | State-of-the-Art Symposium | Bayview A |
Advances in our understanding of cellular biology and cellular architecture have been achieved, in large part, because of innovative engineering in microscopic technology. Today, more than ever, the microscope coupled with laser technology has become a powerful tool in the study of molecular processes and pathways at the single cell level. Proteomic and genomic techniques are dependent on obtaining homogeneous cell populations. Laser Capture Microdissection enables the directed isolation of pure populations of cells from heterogeneous samples. This technology allows cells, cell aggregates, or discrete morphological structures to be selected and captured from tissue sections. These captured cells are suitable for nucleic acid studies such as SNP analysis, endpoint and real-time RT-PCR and mRNA expression profiling. Differential protein-expression profiling by SDS-PAGE and 2D-PAGE as well as protein identification by mass spectrometric sequencing, peptide mass fingerprinting, in-gel zymography and Western blotting also can be carried out with laser microdissected cells. This SID and workshop will illustrate the range of downstream applications in pathology, cancer research, life sciences, medical diagnostics and biotechnology for Laser Microdissection Technology as well as offer a practical view of new advances in instrumentation and sample handling.
10:30 | Introduction (A. Vidrich) | |
10:45 | SA-10 |
Laser Capture Microdissection and Analysis of Gene Expression of Airway Mucous and Serous Gland Cells
Walter Finkbeiner, University of California – San Francisco |
11:15 | SA-11 |
Profiling of Microdissected Gastric Epithelial Cells Reveals a Cell Type Specific Response to Helicobacter pylori Infection
Anne Mueller, Stanford University |
11:45 | SA-12 | Use of Laser Capture Microdissection to Isolate Plant Cells Lewis Feldman, University of California – Berkeley |
MICROSCOPY WORKSHOP
Convener: Alda Vidrich, University of Virginia Health Sciences Center
12:30 pm – 2:30 pm | State-of-the-Art Workshop | Bayview A |
12:30 | Introduction (A. Vidrich) | |
12:45 | W-4 | AS LMD New Advances in Laser Microdissection Technology Andy Lee, Leica Microsystems |
1:15 | W-5 | Sample Preparation and Molecular Analysis for Laser Microdissection Samples Janice Zhou |
1:45 | Hands-on Demo |
THE FUTURE OF IMPROVED NUTRITION THROUGH MODERN BIOTECHNOLOGY
Convener: Raymond D. Shillito, Bayer CropScience
2:30 pm – 4:30 pm | Plant Symposium | Grand Ballroom B |
Ensuring an ample and healthy food supply is needed for a number of reasons: a growing world population, rising consumer demand for improved food quality and safety, and an increasing interest in nutritional enhancements to foods. Traditional plant breeding has been successful in at least partially meeting these goals, and modern agricultural biotechnology is proving to be a powerful complement to traditional methods. This session will give an overview of the state of affairs regarding improvement of crops for improved nutrition, both by agricultural biotechnology and traditional means. It will cover the essentials of the process by which they are evaluated for their potential impact on human/animal nutrition and health, and describe new efforts to apply both traditional and modern methods for nutritional improvement of crops for the developing world.
2:30 | Introduction (R. Shillito) | |
2:45 | P-35 | Improved Nutrition Through Modern Biotechnology Martina Newell-McGloughlin, University of California at Davis |
3:15 | P-36 | Assessment Process for Nutritionally Enhanced Food and Feed Crops Bruce M. Chassy, University of Illinois at Urbana-Champaign |
3:45 | P-37 | HarvestPlus: Biofortification as a Feasible Means of Increasing the Availability of Nutrients to Malnourished Populations Ray Glahn, Cornell University |
PLANT REGENERATION AND MICROPROPAGATION
Moderator: Susan J. Murch, National Tropical Botanical Garden
2:30 am – 4:45 am | Plant Contributed Paper Session | Grand Ballroom C |
2:30 | P-1016 |
Photoautotrophic Medium and PPMTM Help to Alleviate Losses from Bacterial Contamination in Papaya Micropropagation
Maureen M. M. Fitch, USDA/ARS, T. Leong, N. Saito, G. Yamamoto, A. Dela Cruz, A. Yeh, S. White, S. Maeda, and P. Moore |
2:45 | P-1017 | In Vitro Conservation of Endangered Plants in Hawaii Susan J. Murch, National Tropical Botanical Garden |
3:00 | P-1018 |
Plant Regeneration of Taro (Colocasia esculenta (L.) Schott) via Somatic Embryogenesis and Organogenesis
Virendra Mohan Verma, College of the Marshall Islands, and John J. Cho |
3:15 | P-1019 | In Vitro Micropropagation of Ginger (Zingiber officinale Rosc.) and Turmeric (Curcuma longa L.) Xiaoqiang Ma, University of Arizona, and David R. Gang |
3:30 |
P-1020 |
Qualitative and Quantitative Monitoring of Gene Expression Patterns Among Micropropagated Plants of Tea Clones by Differential Screening of Randomly Amplified cDNAs Using RAPD Primers Soom Nath Raina, University of Delhi, and S. Goel |
3:45 | P-1021 | Micropropagation Saves Threatened Citrus Germplasm in Kosrae Puthiyaparambil Chacko Josekutty, College of Micronesia – FSM, E. B. Langu, S. S. Cornelius, T. N. Kilafwasru, and N. S. Nena |
4:00 | P-1022 | Somatic Embryogenic Response of Petiveria alliacea Leaf Explant-derived Callus to Sucrose and Glucose Enriched Media Supplemented with NAA and BAP Seymour Augustus Webster, University of the West Indies, S. A. Mitchell, and M. H. Ahmad |
4:15 | P-1023 | Conservation of Endangered Species Through Plant Tissue Culture in Mexican Botanical Gardens V. Chávez, Jardin Botanico Francisco Javier Clavijero, Victor Manuel Chávez-Avila, A. Martínez, R. Baltazar, E. Pérez, V. Salazar, P. Ortega, and R. Litz |
4:30 | P-1024 | Eugenol Content of In Vitro and In Vivo Ocimum Species – A Comparative Gas Chromatographic Analyses Sivaramakrishnan Sudhakaran, Asian Institute of Medicine, Science and Technology, and V. Sivasankari |