2022 Program

Below is the current program for the 2022 In Vitro Biology Meeting

This program is subject to change.

Saturday, June 4

8:30 PM – 10:00 PM

Design of Experiments Workshop

WORKSHOP

Conveners: Uyen Cao Chu, Corteva Agriscience, Randall P. Niedz, USDA/ARS, and Todd Jones, Corteva Agriscience

Determining optimal formulations and conditions for improved in vitro growth is often done by testing one ingredient or set of conditions at a time. Design of Experiment (DoE) methodology is a particularly useful set of techniques that allows testing of multiple compounds, processes or environmental conditions simultaneously in multiplex systems. The principles and statistics behind DoE are complex, however, and proper experiment design and data analysis relies on computer programs designed specifically for DoE applications. In this workshop, the audience will get an introduction to the use of the principles and tools of DoE design by participating in the design and data analysis of prepared examples relevant to various in vitro culture scenarios.


Sunday, June 5

8:00 AM – 10:00 AM

Advanced Applications of Site Directed Nucleases

PLENARY SYMPOSIUM

Conveners: Durga Attili, University of Michigan Medical School, Pierluigi Barone, Corteva Agriscience, Raj Deepika Chauhan, Pairwise, and Michael Dame, University of Michigan Medical School

Speakers:
Kimberly Cooper, University of California, San Diego
Sergei Svitashev, Corteva Agriscience
Kiana Aran, Keck Graduate Institute

In the last two decades the development of different site directed nucleases (e.g. ZFNs, TALENs and CRISPR-Cas) has provided tools to precisely and efficiently introduce a variety of genetic modifications in a broad spectrum of cell types and organisms. The most recent deployment of these programmable nucleases is represented by new applications including CRISPR gene drive, targeted induction of chromosomal rearrangements and diagnostic screening for genetic mutations. In this session a comprehensive review and exciting recent developments in this area will be presented.

10:30 AM – 12:30 PM

Frontiers in Gene Editing for Crop Improvement

PLANT SYMPOSIUM

Conveners: Fredy Altpeter, University of Florida – IFAS, and Yiping Qi, University of Maryland-College Park

Speakers:
Synthetic Directed Evolution in Plants: Unlocking Trait Engineering and Improvement
Magdy M. Mahfouz,
KAUST, Saudi Arabia
Developing Highly Efficient Base Editing and Prime Editing Tools in Plants
Yiping Qi,
University of Maryland – College Park
Gene Editing in Plants Using Plant Viruses
Savithramma Dinesh-Kumar,
University of California – Davis
Restoring Diversity in the Food System through Inari’s SEEDesign™ Platform Catherine Feuillet, Inari Agriculture Inc.
Catherine Feuillet, Inari Agriculture Inc.

The development of innovative CRISPR/Cas gene editing technologies is refining the speed and precision of plant breeding as urgently needed to feed a growing population on a warming globe. Topics covered in this session will include the development of improved approaches and tools for delivery of gene editing reagents to crops, advances with the precision technologies base editing and prime editing supporting targeted nucleotide substitutions, as well as strategies and examples of gene editing and synthetic directed evolution for crop improvement.

10:30 AM – 12:30 PM

Organoid Technology

ANIMAL SYMPOSIUM

Convener: Terry Riss, Promega Corporation, and Kristina Martinez-Guryn, Midwestern University

Speakers:
Introducing a ‘Phase 0’ in Clinical Trials with Precise Organoid-based Disease Models
Courtney Tindle, University of California, San Diego
James M. Clinton, American Type Culture Collection
Pinar Mesci, University of San Diego

The use of organoids has grown rapidly over the past several years. As a 3D culture model system, they have been demonstrated to more closely represent in vivo biology compared to cells cultured as a monolayer on plastic. The technology to produce organoids has improved greatly, resulting in protocols to generate organoids representing a variety of tissue types. There are many definitions of organoids; but a key element is they are derived from stem cells or organ progenitors that are driven to differentiate into several cell types that self-organize similar to the process in vivo. The speakers in this Symposia will present a general overview of organoids and provide examples of their generation and practical applications to advance our understanding of biology.

10:30 AM – 12:30 PM

Advances in Double Haploid Technology

PLANT SYMPOSIUM

Conveners: Cliff Hunter, Consultant, and Chuck Armstrong, Plastomics

Speakers:
Accelerating Precision Breeding through Double Haploids
Lorena Moeller, Bayer Crop Science

Breeding Technology & Discovery Progress at Syngenta
Weiguo Liu, Syngenta Seeds Research
Nathanaël Jacquier, Limagrain

Doubled haploid technology is now a cornerstone of plant improvement programs for many of the world’s most important crops, including maize, wheat, oilseed rape, and a wide variety of vegetable / horticultural species. Significant, steady improvement has been made over the years in improving the rate of haploid induction, the identification and selection of haploids, and the efficiency of chromosome doubling and recovery of dihaploid seed. In recent years, important breakthroughs have been made in understanding the molecular basis of haploid induction. These include cloning of a major gene involved in haploid induction in maize and development of a novel chromosome elimination mechanism (modified CENH3), each of which has been demonstrated to work across species. Haploid induction has also been combined with gene editing to increase the speed with which edited traits can be fixed and evaluated in relevant germplasm. In this session, we will bring together representatives of several major seed companies to present selected highlights of recent work on dihaploid technology within their organizations.

1:30 PM – 3:00 PM

Novel Delivery Technologies – Overcoming Bottlenecks

PLANT SYMPOSIUM

Convener: Gözde Demirer, California Institute of Technology

Speakers:
Gregory Lowry, Carnegie Mellon University
Michael Maher, Invaio Sciences
Gözde Demirer, California Institute of Technology

The need to accelerate crop breeding programs has never been greater, as the world population is exponentially increasing, climate is changing, and resources are limited. Breeding relies on genetic variation, be it natural, induced, or introduced. Given the recent development and rapid advances in the field of gene editing through the CRISPR/Cas technology, plant genetic engineering has become a highly promising approach for increasing crop yields and nutritional value, and to generate high-yielding cultivars that are resilient to various biotic and abiotic stresses that can grow with less water and nutrient resources. Despite the recent progress in genome editing, most plant species remain impossible or difficult to genetically engineer, retarding the progress in plant biology research and crop improvement. The two most critical bottlenecks of generating engineered plants are i) biomolecule delivery into plant cells with walls and ii) tissue culture-based plant transformation. The common transformation tools, Agrobacterium and gene gun, are not able to transform plant reproductive organs and germ cells directly, except in a few model species, therefore necessitating regeneration of plants from the transformed explants. Plant regeneration in tissue culture is a low-throughput and laborious process that requires specialized methodology development for each species and genotype, and not all plants are amenable to tissue culture. Moreover, Agrobacterium itself is a highly species-dependent tool, limiting the extension of developed biotechnologies to a broad range of plants. Given these limitations, there is an immense need to develop broadly-applicable and innovative plant delivery and transformation technologies. In this session, we will discuss these new and novel delivery technologies to plants with impactful applications in agriculture and fundamental plant biology research.

1:30 PM – 3:00 PM

Extracellular Matrix and Tissue Engineering

ANIMAL SYMPOSIUM

Convener: Joshua Gasiorowski, Midwestern University

Tissue engineering has had exciting clinical successes, but new translational breakthroughs in the field will be dependent upon researchers fully understanding the properties of endogenous extracellular matrices so they can design biologically relevant mimics. The extracellular matrix (ECM) is a three-dimensional proteinaceous meshwork that provides tensile strength and connective, anchoring material for all tissues. However, the ECM is also a rich source of signals that influence and control cell behaviors. The biochemical composition of collagens, laminins, and other fibrous proteins act as ligands for cell signaling receptors while the biophysical cues in the form of compliance, elasticity, and topography directly mediate independent cellular responses. This session will highlight recent advances in understanding the basic science properties of the extracellular matrix, in addition to discussing ECM mimics that are designed to serve as tissue engineering scaffolds for regenerative medicine.

1:30 PM – 3:00 PM

Plant Growth Regulators

PLANT SYMPOSIUM

Conveners: Geny Anthony, Corteva Agrisciences, and Kristian Adamek, University of Guelph

Speakers:
Yunde Zhao, University of California, San Diego
Sean Cutler, University of California, Riverside

Plant Growth Regulators (PGRs) are fundamental to growth and development of plants at the cellular, tissue and organ levels. PGRs have the capacity to influence cell division, cell expansion, and cell structure and function, in addition to mediating environmental stress. Since the recognition of their functional importance in the 1930s, the main classes of PGRs (such as auxins, gibberellins, cytokinins, abscisic acid and ethylene) have been well researched. Apart from their use as growth enhancers in agriculture and horticulture, PGRs and their synthetic analogues have been extensively used for in vitro manipulation of plant tissues for research and commercial purposes. PGRs rarely act alone and are almost always involved in pathways where multiple PGRs interact. Recent advances in this field have unraveled their complex mechanisms and pathways. Plus, many new structurally similar and/or chemically unrelated compounds with PGR-like effects have been discovered. This session will inform the audience about the latest advances in understanding the complex mechanism and pathways of PGRs.

3:15 PM – 5:30 PM

Opening Ceremony and Keynote Symposium

KEYNOTE SYMPOSIUM

Keynote Speaker:
Advancing Cell Culture to Meet Scientific and Societal Needs
Thomas Hartung, MD, PhD, Director of the Center for Alternatives to Animal Testing (CAAT) and inaugural holder of the Doerenkamp-Zbinden Endowed Chair in Evidence-based Toxicology in the Department of Environmental Health Sciences at the Johns Hopkins Bloomberg School of Public Health


7:30 pm – 9:30 pm

Student Workshop: State-of-the-Art Cell Imaging Technologies

EDUCATION SYMPOSIUM

Conveners: Mubeen ul Hasan, Nigde Omer University, and Muneeb Hassan Hashmi, Nigde Omer Halisdemir University

Since the earliest microscopy efforts in the 1600s, cell imaging has significantly modernized and became a ubiquitous tool in cell biology laboratories. Light microscopy was the earliest technique used in biology and has remained a main staple of cell biology and continues to evolve into more advanced techniques, which has been fueled by developments in computation, image detection devices, labelling, and sample preparation strategies. This student-sponsored workshop is to provide hands-on and virtual experiences in some of the latest imaging technologies.


Monday, June 6

8:00 AM – 10:00 AM

Future Foods

PLENARY SYMPOSIUM

Conveners: Vivian Dayeh, University of Waterloo, Veena Veena, Donald Danforth Plant Science Center, and Sukhpreet Sandhu, HM. Clause

Speakers:
Bruce Friedrich, The Good Food Institute
Brandon Chen, Finless Foods

Aletta Schnitzler, Turtle Tree
Alan Perlstein, California Cultured

Meats grown in a petri dish, chocolate produced without cacao trees, and milk produced without ever stepping on a farm. Is this the future of the foods we eat? Increasing threats to world’s food production systems and the need to creating sustainable food products and reducing carbon emissions from agriculture, has been driving the growth of plant based and cell based foods. One of these emerging areas is cellular agriculture that relies on in vitro methods instead of whole plants or animals for food production. The Future Foods plenary session will present cutting edge research on alternative food sources including plant and animal cell cultures and how the technology will change the food system in the future. The speakers in this symposium will excite the audience on the future of alternative seafoods, chocolate, and milk products, and how these innovations will revolutionize agriculture.

10:30 AM – 12:30 PM

Plant Biotechnology Post-Doctoral Oral Presentation Competition

PLANT CONTRIBUTED PAPER SESSION

Moderator: Massimo Bosacchi, HM. Clause

10:30 AM – 12:30 PM

Plant Biotechnology Student Oral Presentation Competition

PLANT CONTRIBUTED PAPER SESSION

Moderator: Alex da Silva Conceicao, Calyxt

To support the Society’s vision to encourage education and scientific informational exchange, the Plant Biotechnology Section is pleased to announce the “Plant Biotechnology Student Oral Presentation Competition” at the 2022 World Congress on In Vitro Biology being held from June 4-7, 2022 in San Diego, CA at the Town and Country San Diego. Students wishing to participate in this competition should submit a copy of their abstract with its title and submission ID number to Alex da Silva Conceicao ( alexandre.dasilvaconceicao@calyxt.com ) and check that option when they submit their abstract. Student competition finalists will be selected based on the quality of the abstracts. The abstract should address the following: Background, Objectives, Methods, Results, Discussion and Conclusions. Where appropriate, the methods section should include a description of how reproducible results were ensured. The abstract must not include references. The abstract text must not exceed 1800 characters. A panel of judges will evaluate the presentations at the meeting. Criteria for the evaluation include experimental design, data analysis, proper interpretation of the results, originality of the study, technical difficulty, appearance, and ability of the candidate to communicate the work. Winners will be presented with a certificate and a cash award at the meeting. Please note that the DEADLINE to submit your abstract for the SIVB Oral Presentation Competition is January 31, 2022.

10:30 AM – 12:30 PM

Moving the Field Forward: Application of New Technologies in Academic, Industry and Regulatory Agencies Perspectives

ANIMAL SYMPOSIUM

Conveners: Addy Alt-Holland, Tufts University, Kenneth Kandaras, International Foundation for Ethical Research, and John Harbell, JHarbell Consulting

12:30 PM – 1:30 PM

Student Networking Luncheon: Research Proposal and Grant Writing

EDUCATION WORKSHOP

Moderators: Mubeen ul Hasan, Nigde Omer Halisdemir University, and Muneeb Hassan Hashmi, Nigde Omer Halisdemir University

Have you been looking for how to write a successful research proposal and how to get funding for your research as a graduate student? Have you been searching for how to find the right funding agency for your research or even thought about writing a proposal that will be accepted by your funding agency or your supervisor? Or are you confused about how to target the key areas of a topic that will make your research proposal interesting for the jury and funding agency? If that’s the case, this is your golden opportunity! Come to the student networking luncheon to find all the answers you have been waiting for. We are putting together a panel of researchers that will answer all of your questions and will help alleviate any confusion. If you are a new Master’s or Ph.D. student who needs to write a proposal, then researchers will be available to assist you and provide you with information about how to find the perfect topic for your research as well as all the material that you need to write a proposal. If you are a student and you need to find a public or private funding agency to fund your research, our professionals will help you understand the requirements of the various funding agencies. Similarly, if you want to apply for a scholarship to study abroad and your scholarship requires a strong research proposal, this panel will tell you how they chose students for these positions which can help you get answers to your questions. Professionals will also share their academic experiences and discuss how they coped while pursuing their graduate degrees. This workshop may also introduce you to your new research mentor, supervisor, collaborator, and/or external internship opportunity.

1:30 PM – 2:30 PM

In Vitro Animal Cell Sciences Student and Post-Doctoral Oral Presentation Competition

ANIMAL CONTRIBUTED PAPER SESSION

Moderators: Addy Alt-Holland, Tufts University, and Kolla Kristjansdottir, Midwestern University

The In Vitro Animal Cell Sciences Section (IVACS) of the Society for In Vitro Biology is pleased to announce the 2022 Student and Post-Doctoral Oral Presentation Competition during the SIVB meeting in San Diego, CA. This competition encourages the exchange of scientific information between the presenters, attendees and judges. Additionally, it provides an invaluable opportunity for students and post-docs to practice and improve their presentation delivery and public speaking skills. Students and post-docs who wish to participate in this competition should check that option during the submission of their abstract to the 2022 SIVB’s meeting. Applicants should also e-mail a copy of their complete abstract and submission ID number to the session moderators, Dr. Addy Alt-Holland (addy.alt_holland@tufts.edu) and Dr. Kolla Kristjansdottir (kkrist@midwestern.edu). The top three finalists will be selected for this competition based on the quality of their abstracts, as well as the merit of their research and scientific findings. The text of the abstract should include the following sections: Background, Objectives, Methods, Results, Discussion and Conclusions. Where appropriate, the Methods section should include relevant statistical analysis. The abstract text should not exceed 1800 characters and should not include references. The oral presentations will be discussed and graded by a panel of expert judges. Evaluation criteria will include experimental design, data analysis, proper interpretation of the results, originality of the study, technical difficulty, professionalism and ability of the finalist to explain the research and answer questions, and importantly, adherence to the allocated time for the presentation. The Student and Post-Doctoral Oral Presentation Competition session serves to recognize and reward the research and achievements of outstanding students and post-docs. The three finalists will be presented with a certificate and a cash award during the meeting. The DEADLINE for abstract submission for the Student and Post-Doctoral Oral Presentation Competition is January 31, 2022. Should you have any questions, please contact the SIVB office or the session moderators. We are looking forward to review your abstract!

3:30 PM – 5:00 PM

Alternative Approaches to Plastid Engineering

PLANT SYMPOSIUM

Convener: Jeffrey Staub, Plastomics

Speakers:
Agrobacterium-mediated Transformation of the Plastid Genome
Pal Maliga, Rutgers University
Modification of the Plastid Genome Using Base Editing
Shin-Ichi Ariumura, The University of Tokyo

Stable plastid (chloroplast) transformation technology was developed more than 30 years ago. The technology has been used extensively to study plastid biology and to introduce a variety of traits with benefits to growers and consumers alike. However, only a limited number of model plant species are routinely transformed and no plants carrying engineered plastids have yet been commercialized, in part due to complexities of the technology and bottlenecks in tissue culture of agronomically important crops. More recently, alternative technologies are emerging as potential ways to engineer plastids that circumvent some of the previous challenges. This session will highlight some of those emerging technologies for plastid engineering and will discuss novel traits developed using those technologies.

3:30 PM – 5:00 PM

Non-competitive Student Oral Presentations

EDUCATION CONTRIBUTED PAPER SESSION

Conveners: Mubeen ul Hasan, Nigde Omer Halisdemir University, and Muneeb Hassan Hashmi, Nigde Omer Halisdemir University

The Society for In Vitro Biology (SIVB) takes pride in showcasing some of the most innovative research topics in both plant and animal sciences. Our mission for the non-competitive oral presentations is to provide a platform for student researchers to achieve recognition and share their research work with their peers as well as academic and industry professionals, who can take their projects to the next level without the pressure of competing. This section is designated for those who are looking to gain experience in presenting scientific information, as well as for those who are developing effective scientific presentation skills.

3:30 PM – 5:00 PM

New Approaches to Vaccine Development

JOINT SYMPOSIUM

Conveners: Julie Swartzendruber, Midwestern University, Barbara B. Doonan, New York Medical College, and J. Pon Samuel, Corteva Agriscience

Speaker:
Protection from SARS-CoV-2 Infection and Transmission Using Vaccines and Therapeutics Made in Plant Cells
Henry Daniel, University of Pennsylvania

This joint symposium will focus on new developments and issues related to the use of vaccines. The rapid development and deployment of the COVID-19 vaccine has generated a lot of interest around how vaccines are developed. This session will invite expert faculty to discuss the latest information on vaccines including the future of vaccine development and surveillance.

5:00 PM – 6:00 PM

IVACS Contributed Paper Session

ANIMAL CONTRIBUTED PAPER SESSION

Moderator: Mae Ciancio, Midwestern University

5:00 PM – 6:00 PM

Plant Contributed Paper Session I

PLANT CONTRIBUTED PAPER SESSION

Moderator: TBD

5:00 PM – 6:00 PM

Plant Contributed Paper Session II

PLANT CONTRIBUTED PAPER SESSION

Moderator: TBD

8:30 PM – 10:00 PM

Public-Private Partnerships for Sustainable Innovation Workshop

WORKSHOP

Convener: Sukhpreet Sandhu, HM. Clause, and Kathy Munkvold, Corteva Agriscience

The global scientific community can do truly innovative work when we collaborate. Public-private partnerships (PPPs) between government agencies, universities, and other public research institutions with private sector companies fuel innovation by bringing unique capabilities and diverse thinking together to solve important challenges. Through common objectives, shared benefits and complementarity of human and financial resources, PPPs can connect the dots between a technology or solution to a problem and deploying that solution at scale. PPPs are increasingly used (in ag and biotech innovation) to enhance efficiency and accelerate innovation while fostering wider and faster uptake by leveraging public funds, mobilizing policy or government action. However, the success rate of many PPPs is variable with some failing to deliver the expected value both for the partners and the stakeholders. In this workshop, we will dive into common pitfalls for PPPs, risk management, best practices and guidelines on how to build PPPs for sustainable innovation.


Tuesday, June 7

8:00 AM – 10:00 AM

Artificial Intelligence and Machine Learning in Plant and Animal Sciences

PLENARY SYMPOSIUM

Conveners: Mae Ciancio, Midwestern University, Annie Saltarikos, Bayer U.S. – Crop Science, and Evan Hill, University of Michigan

10:30 AM – 12:30 PM

Advances in Cannabis Biotechnology

PLANT SYMPOSIUM

Conveners: Ian Cole, Zenlabs

Speakers:
Jeffrey Adelberg, Clemson University
Hope Jones, Emergent Crop Sciences (ECS)
Chris Leavitt, Node Labs
Joseph Ramahi, Apikal Biotek

The application of tissue culture and biotechnology is becoming a vital component of Cannabis businesses, especially as the market grows globally. Recent studies have found that plant pathogens have led to billions of dollars in damages to the Cannabis industry in North America, and advances in Cannabis Biotechnology have been identified as a potential solution to some of these problems. Additionally, the industry has had to innovate and draw from research and academia to fulfill the skyrocketing demand for healthy plants. This session brings together industry and academic researchers who are actively attempting to understand the challenges faced by current Cannabis businesses and the gaps that remain in our understanding of the horticulture and biology of Cannabis in the modern context. We hope to present information on treating pathogens (especially hop latent viroid), advances to Cannabis tissue culture techniques and advances in acclimatization of lab-grown plants back to greenhouses to be ready for cultivation.

10:30 AM – 12:30 PM

The Art and Science of Cell Imaging

ANIMAL SYMPOSIUM

Conveners: Brad Upham, Michigan State University, Debora Esposito, North Carolina State University, and Cynthia L. Goodman, USDA/ARS/BCIRL

Student Conveners: Muneeb Hassan Hashmi, Nigde Omer Halisdemir University, and Mubeen Hasan, Nigde Omer Halisdemir University

Since the earliest microscopy efforts in the 1600s, cell imaging has significantly modernized and became a ubiquitous tool in cell biology laboratories. Light microscopy was the earliest technique used in biology and has remained a main staple of cell biology and continues to evolve into more advanced techniques, which has been fueled by developments in computation, image detection devices, labelling, and sample preparation strategies. Some technological advances in this field include single molecule localization microscopy for imaging of living cells at or near nanometer spatial resolution. CRISPR-labeled fluorescence imaging of 3D structure of the genome in live cells. Brillouin microscopy to probe the viscoelastic properties of biological samples with diffraction-limited resolution in 3D. Holo-tomographic microscopy that quantitates phase microscopy into a hologram. High content-analysis microscopy that can track cells in 2D over time as they grow continuously over extended times by integrating microscopy inside incubators. Light sheet fluorescence microscopy that is designed to illuminate only the thin imaging focal planes, thus minimizing out-of-focus fluorescence and photobleaching. In this session speakers will discuss some of these state-of-the-art imaging methods to evaluate different aspects of cell biology including signal transduction, regulatory responses to stress, and disease states.

10:30 AM – 12:30 PM

Model Systems for Developing CRISPR/Cas Technology in Plants

PLANT SYMPOSIUM

Convener: Shubha Subbarao, Bayer U. S. – Crop Science, and Jeffrey Beringer, Inari Agriculture

Genome editing has the potential to speed up the pace of plant breeding and trait development. As researchers seek to gain a greater understanding of CRISPR/Cas and related technologies the need to rapidly test the effectiveness of a multitude of editing concepts has become a pressing need. Rapid assay systems provide the opportunity to evaluate large numbers of constructs without engaging in the lengthy regeneration of mature plants, thereby helping manage costs and resources effectively.

3:30 PM – 5:00 PM

Genetic Transformation and Regeneration of Recalcitrant Species (i.e., Fruit Trees, Orphan Crops), Challenges and Way Forward

PLANT SYMPOSIUM

Convener: Carlos M. Hernandez-Garcia, CTC Genomics, Juan Debernardi, University of California, Davis, and Pamela Vogel, Pairwise

Speakers:
Of Media and Miracles: Successes and Frustrations in the Search for Efficient Regeneration and Transformation Methods for Trees and Crops
Steven Strauss, Oregon State University
A CRISPR-combo Approach for Speed Breeding and Regeneration of Genome-edited Plants
Yiping Qi, University of Maryland-College Park
Efficient Plant Regeneration from Protoplasts Isolated from Multiple Grape Genotypes and Demonstration of PEG-mediated Gene Editing Using CRISPR/Cas9
David Tricoli, University of California-Davis

Plant genetic transformation has been fundamental to enable basic research in plant biology and has revolutionized commercial agriculture. Unfortunately, robust, reproducible, and efficient plant transformation methods remain highly genotype-dependent and have been limited to a few species and selected genotypes of major crops. Genome editing provides novel opportunities for both basic research and product development; however, its rapid adoption within the scientific community has clearly evidenced major bottlenecks in the transformation and regeneration processes. Interestingly, major recent breakthroughs in the use of developmental genes such as GRF, BBM, WUS have led to dramatic improvements in transformation and regeneration efficiencies in monocots and have broaden the number of species and genotypes amenable to transformation. This revives the hope for the development of more universal and genotype independent transformation protocols. The use of these developmental genes is also expected to be expanded to more local crops where recalcitrance is often a significant challenge for genetic engineering, further enabling research groups with less specialized expertise to succeed in transformation. In this session we will review the status of the transformation of recalcitrant crops and discuss about potential future developments in the field.

3:30 PM – 5:00 PM

In Vitro Technologies for Plant Conservation and Gene Banking

PLANT SYMPOSIUM

Conveners: Valerie C. Pence, Cincinnati Zoo and Botanical Garden, and Lori Marcum, Corteva Agriscience

Speakers:
In Vitro Methods for the Conservation of Rare Plants of the Southeastern U.S.
Emily Coffey, Atlanta Botanical Garden
In Vitro Methods for the Conservation of Threatened Magnolia Species
Raquel Folgado, The Huntington Library, Art Museum, and Botanical Gardens
Micropropagation in the Hawaiian Rare Plant Program
Devon Gordon, The University of Hawai’i at Mãnoa

With the increasing threats of habitat loss, unsustainable use, and climate change to the world’s plant biodiversity, the need for ex situ conservation of plant genetic resources is also increasing. This is most efficiently accomplished with seed banks, but there are predicted to be tens of thousands of species for which conventional seed banking will be unworkable. These species are collectively known as exceptional plants and most will require cryopreservation methods in order to conserve them long-term. In vitro methods will be critical for many of these species, for providing tissues for cryopreservation and for propagating and recovering plants to restore species in the wild. Thus, there is an urgent need for developing in vitro protocols for a wide range of threatened plant taxa. This session provides a look at the work of three laboratories that are focused on this goal, representing a variety of species and illustrating the approaches and challenges of this work.

3:30 PM – 5:00 PM

Single Cell Technology and Application in Biomedical Research

ANIMAL SYMPOSIUM

Conveners: Kristina Martinez-Guryn, Midwestern University, and Rosa Ventrella, Midwestern University

Speakers:
John Chang, University of California, San Diego
Scott Atwood, University of California, Irvine
Utilizing Single Cell Analyses to Characterize Models of Human Cortical Organoids
Aparna Bhaduri, University of California, Los Angeles

Single-cell omics technologies, such as genomics, epigenomics, transcriptomics, proteomics, and metabolomics have undergone rapid growth over the past decade. These technologies can analyze many cells at a high resolution making it a valuable tool in characterizing rare cell types and better understanding diverse cell states. Studying the cell-cell variation within cell populations can reveal unique mechanisms related to disease pathogenesis. The goal of this session is to describe the current state of research utilizing single-cell technologies and provide key examples of how these technologies are being used to identify how cellular heterogeneity contributes to human disease.