Below is the current program for the 2020 World Congress on In Vitro Biology.

This program is subject to change

Saturday, June 6

}

9:00 am – 5:00 pm

Grow with the Flow: Creative Change on Advanced Flow Cytometry

Convener: J. Pon Samuel, Corteva Agriscience

Advanced Registration is required to participate in this workshop

In continuation of the success of Flow Cytometry workshop in the last two years, this year’s Grow with the Flow workshop will focus on various advanced areas in flow cytometry and fluidics for targeting and gene editing with specific biological applications. Experts will present and be available for discussions on these advance areas including characterization of nano conjugates using flow cytometry.  In addition, presentations on technology updates and Flow Cytometry best practices will be discussed. During the workshop a panel would help with the tips on interpretation of results on flow data with the world expert who invented the high-speed flow cytometry. Following the workshop, experts will be available to answer questions on the capabilities of the technology and the equipment in addition to any other relevant information at the exhibition hall during the rest of the conference.

 

}

9:15 am – 5:30 pm

15th International Conference on Invertebrate and Fish Cell Culture

Organizers: Lucy Lee, University of the Fraser Valley; Cynthia Goodman, USDA, ARS, BCIRL; Guy Smagghe, Ghent University; Kavita Bitra, BASF; and Jessica Monserrate, BASF

9:15 am – 12:15 pm
Cellular Aquaculture/Agriculture

Convener: Lucy Lee, University of the Fraser Valley

Speakers:
The Importance of Basic Research on Cellular Physiology for Improving Cellular Aquaculture Applications
Martin Tresguerres, The Scripps Institution of Oceanography
Using Zebrafish to Optimize Cellular Agriculture
David Stachura, California State University, Chico
Cell Line Development from Finfish for Manufacturing of Cell-based Seafood
Lauran Madden, BlueNalu, Inc.
Seafood without the Catch – Advancing R&D Toward Product Launch in the Cellular Agriculture Industry 
Brandon Chen, Finless Foods
Insect-based Tissue Engineering for Food
Natalie Rubio, Tufts University

Cellular agriculture is the implementation of cell and tissue culture systems to produce agricultural products in place of whole plants or animals. A variety of these systems (involving fish and invertebrates) and potential products will be explored. We will discuss the engineering of insect tissues for use as food sources. The benefits of aquaculture will be examined, looking at the practical potential of selected marine products. The development of technologies leading to the production of seafood (without the negative environmental impacts that can come from over-fishing) will also be reviewed, with an emphasis on sustainability and affordability.

 

1:55 pm – 4:30 pm
Monitoring and Screening of Contaminants and Bioactives

Conveners: Cynthia Goodman, USDA, ARS, BCIRL, and Guy Smagghe, Ghent University

Speakers:
Use of Fish Cell Lines to Assess the Toxicity of Heavy Fuel Oil (HFO)
Niels Bols, University of Waterloo
The Development of New Genomics and Cell Biology Tools for the Atlantic Horseshoe Crab
Rachel O’Neill, University of Connecticut
Cell Culture System: Production of an Infectious Clone of Dicistrovirus and Iflaviruses
Kavita Bitra, BASF Corporation
TBD
Guy Smagghe, Ghent University
Advances in Insect Cell Engineering Approaches with Potential Application for Monitoring and Screening Contaminants and Bioactives
Donald Jarvis, University of Wyoming

Fish and invertebrate cell cultures serve as valuable tools in the detection of environmental pollutants and the development of agriculturally relevant products. Our session will focus on the deployment of several culture systems for these purposes and consider ways to modernize techniques to improve relevancy for industrial and environmental applications. We will hear about the use of fish cell lines to accurately assess the impact of potential toxins on the aquatic environment. This will be followed by a discussion on the study of genome biology of marine organisms aimed at understanding the dynamic response of the genome to environmental queues, such as global warming. The development of cell cultures from marine invertebrates will also be covered. Our next speaker will describe the cloning of RNA viruses, infectious to insects, via cell lines ultimately to generate effective biological control agents. We will then hear about the use of cell cultures to evaluate the impact of cellular uptake of dsRNA (involved in RNAi) and in order to increase the potency of RNA-based insecticides. Our last speaker will discuss state-of-the art molecular approaches important in the development of novel cell culture systems useful in both basic and applied research efforts.

 

4:30 pm – 5:30 pm
Panel Discussion

Moderators: Lucy Lee, University of the Fraser Valley, Cynthia Goodman, USDA, ARS, BCIRL, and Guy Smagghe, Ghent University

Conference speakers will serve as panelists during this discussion period. Topics to be covered will include those introduced during the presentations as well as related topics, such as cell line initiation strategies and media development.

 

Sunday, June 7

}

8:00 am – 10:00 am

20/20 on 2020 and Beyond:  Emerging In Vitro Technologies

Conveners: J. Pon Samuel, Corteva Agriscience, Mike Mann, Pairwise, Michael Dame, University of Michigan Medical School, and Durga Attili, University of Michigan Medical School

Speakers:
Universal Mechanisms Controlling Biomineralization in Microbes, Plants and Animals
Bruce W. Fouke, University of Illinois Urbana-Champaign
Beyond Basepairs: Integrative Genome Science at the JGI
Nigel
J. Mouncey, DOE Joint Genome Institute
Modeling Epithelial-Mesenchymal Plasticity in 3D Epithelial Organoids
Jing Yang, University of California, San Diego

 

This session captures the remarkable diversity of innovative in vitro models, recapitulating complex systems across phyla and function. We’ll learn about modeling the process of biomineralization which impacts a vast range of vital processes. Living cells biologically regulate mineral production and deposition as characterized in plant, animal and microbiota. Then to interrogating the complex world of metabolomics with novel genomic platforms. Lastly, organoid models have demonstrated significant potential to explore a multitude of questions to which could not be addressed with previously available cell lines and animal models. 3-D organoids reproduce many of the central aspects of normal and neoplastic growth including polarized expression of differentiation and stem cell markers. These discussions will introduce some exciting new capabilities for the future of in vitro modeling.

}

10:30 am – 12:30 pm

Best Practices for Plant Tissue Culture

Conveners: Valerie Pence, Cincinnati Zoo and Botanical Garden, and Maria Jenderek, USDA-ARS

Speakers:
Conservation of Magnolia Spp Using Cryobiotechnology: From Wild Collection to Ex Vitro Hardening
Raquel Folgado, The Huntington Library, Art Collections and Botanical Gardens
Bumps, Potholes and Crashes Along the Way to Successful Micropropagation: Prevention and Intervention
Carolyn Sluis
, Tissue Grown Corporation
Best Practices for Explant Selection, Initiation, Stabilization and Contaminant Indexing
Michael E. Kane, University of Florida
Overview of Plant Tissue Culture Media and Practices
Gregory C. Phillips, Arkansas State University

While there are many uses for plant tissue culture, there are some practices, principles, and challenges common throughout plant in vitro systems. This session will explore some of these including dealing with contaminants during culture initiation, the characteristics of different media and plant growth regulators, examples of application of these practices to specific plant taxa, as well as some of the major hazards to in vitro work in industry and the strategies for avoiding, minimizing, and overcoming them.

 

Genetic Transformation and Accelerated Breeding in Woody Plant Species

Conveners: Sadanand Dhekney, University of Maryland Eastern Shore, and Song Zhang, Pairwise

Speakers:
Flowering Mechanism in Woody Plants: Manipulation of Blueberry Flowering Pathway Genes for Accelerated Breeding and Yield Increase
Guoqing Song, Michigan State University
Jayasankar Subramanian, University of Guelph
Terrence Frett, Sun World Innovations
Ralph Scorza, USDA-ARS

Perennial woody tree species are considered to be recalcitrant to plant regeneration and genetic transformation. The complex genetic background of cultivars makes the transformation very genotype-dependent. Additionally, a long juvenile period for woody plant species makes the breeding of fruit and forest trees more time-consuming compared to the time required for breeding annual crops. It can take more than 15 to 20 years to breed new fruit tree cultivars. Based on modern technologies, flowering mechanism and key genes to shorten tree juvenility have been discovered and utilized in woody plant breeding. Our invited speakers will share their perspective, experience and knowledge in woody plant genetic transformation and accelerated breeding techniques for the development of improved cultivars.

 

Organoid Models: Windows into Human Disorders

Conveners: Durga Attilli, University of Michigan Medical School, and Michael Dame, University of Michigan Medical School

Speakers:
Integrative Modeling of Human Brain Development and Neurodevelopment Disorders
Bennett Novitch, David Geffen School of Medicine at UCLA
“Gut-in-a-Dish” Model for Developing Personalized Therapies for Chronic Diseases
Soumita Das, University of California, San Diego
From Brain Organoids to Animal Chimera: Novel Platforms for Studying Human Brain Development and Disease
Abed Alfattah Mansour, The Salk Institute for Biological Studies

Recent improvements in stem cell technology allow differentiation of pluripotent stem cells derived from patient skin cells to form 3D multicellular organoids to study human development and disease. These techniques advanced the ability to grow stem cell derived brain organoids, “mini brains in a dish”, and offered unparalleled opportunity to study human brain development and mechanisms of disease that previously lacked reliable model systems. In this session, speakers will present data from recently pioneered human brain organoid systems to understand the basis of neurodevelopmental disorders, molecular mechanisms controlling developmental processes, stem/progenitor cell regulation, cell fate decision-making, repair and neuropsychiatric disorders. Finally, work with human gastrointestinal organoids will be presented to demonstrate the novel use of organoid models to develop diagnoses and therapies for a spectrum of chronic gastrointestinal diseases including infectious disease and cancer.Recent improvements in stem cell technology allow differentiation of pluripotent stem cells derived from patient skin cells to form 3D multicellular organoids to study human development and disease. These techniques advanced the ability to grow stem cell derived brain organoids, “mini brains in a dish”, and offered unparalleled opportunity to study human brain development and mechanisms of disease that previously lacked reliable model systems. In this session, speakers will present data from recently pioneered human brain organoid systems to understand the basis of neurodevelopmental disorders, molecular mechanisms controlling developmental processes, stem/progenitor cell regulation, cell fate decision-making, repair and neuropsychiatric disorders. Finally, work with human gastrointestinal organoids will be presented to demonstrate the novel use of organoid models to develop diagnoses and therapies for a spectrum of chronic gastrointestinal diseases including infectious disease and cance

}

12:30 pm – 1:30 pm

Leadership and Best Practices in Commercial Laboratories -21st Century and Beyond

Conveners: Anthony Nwangwu, Duarte Nursery, and Benjamin Hughes

}

1:30 pm – 3:00 pm

New Approach Methods for Drug Discovery in Japan

Conveners: Hajime Kojima, National Institute of Health Sciences and Yohei Hayashi, RIKEN Bioresource Research Center

Speakers:
Collagen Vitrigel Membrane Useful for Fabricating Three-dimensional Culture Models and Their Application to the Development of Test Methods for Predicting ADME/Tox of Chemicals
Toshiaki Takezawa, National Agriculture and Food Research Organization
Drug Discovery Using Disease-specific iPS Cell Collection in RIKEN Cell Bank
Yohei Hayashi, RIKEN Bioresource Research Center
Development of Novel Drug Safety Assessments Using Human iPS Cell Technology
Yasunari Kanda, National Institute of Health Sciences
Consideration Points for the Development of Microphysiological System
Seiichi Ishida, National Institute of Health Sciences

Seeking to become a global leader in the field of stem cell technology since Dr. S. Yamanaka’s Novel prize on the development of human induced pluripotent stem cell (hiPSC) in 2012, the Japan Agency for Medical Research and Development (AMED) has provided support for the research and development into clinical application of regenerative medicine and a useful tool for conducting risk assessments of drug candidates with stem cell technology. In this symposium, we focus the projects on drug discovery with hiPSC under the Japanese funding agency and introduce the current researches of new safety pharmacology test, new scaffolds for the cells, patient-derived iPSC bank and the Microphysiological Systems (MPS).

Plan(t)s for the Future Planet

Conveners: Pierluigi Barone, Corteva Agriscience, and Todd J. Jones, Corteva Agriscience

Speakers:
Deciphering the Molecular and Cellular Development of a C4 Photosynthetic Leaf
Tammy Sage, University of Toronto
Harnessing Plant Biology to Address Climate Change
Wolfgang Busch, The Salk Institute for Biological Studies
Engineering the Nitrogen-fixing Nodulation Trait Using Legumes and the Tropical Parasponia Tree (Cannabaceae) as Templates
Rene Geurts,
Wageningen University & Research

In the coming decades agricultural production faces tremendous challenges in terms of productivity growth and natural resource use efficiency and environmental stewardship. The increased demand for food and feed associated with climatic changes will require the development of plants able to use fertilizers more efficiently, remove more carbon from the environment, and maintain soil and water quality. In this symposium our speakers will share some of the most recent efforts to translate advances in applied plant biology and biotechnology to accelerate crop improvement and sustainability.

 

In Vitro Technologies for Clean Plant Production in Cannabis

Conveners: Max Jones, University of Guelph, and Hemant Lata, Mississippi State University

Speakers:
David Joly, Université de Moncton
Max Jones, University of Guelph
Jeremy Warren, Dark Heart Nursery

}

3:15 pm – 5:30 pm

Opening Ceremony and Keynote Symposium

Emergence of Spontaneous Oscillatory Networks from Human Brain Organoids

Keynote Speaker: Alysson R. Muotri, Professor, Sanford Consortium, University of California, San Diego

The complexity of the human brain, with thousands of neuronal types, permits the development of sophisticated behavioral repertoires, such as language, tool use, self-awareness, symbolic thought, cultural learning and consciousness. Understanding what produces neuronal diversification during brain development has been a longstanding challenge for neuroscientists and may bring insights into the evolution of human cognition. Human pluripotent stem cells have the ability to differentiate in specialized cell types, such as neurons and glia. Moreover, induced pluripotent stem cells can be achieved from living individuals by reprogramming somatic cells that would capture their entire genome in a pluripotent state. From these pluripotent state, it is possible to generate models of the human brain, such as brain organoids. We have been using brain-model technology (BMT) to gain insights on several biological processes, such as human neurodevelopment and evolution. We also applied BMT to measure the impact of genetic variants in autism spectrum disorders and for evolutionary studies. The reconstruction of human synchronized network activity in a dish can help to understand how neural network oscillations might contribute to the social brain. Our findings suggest a potential bridge to the gap between the microscale in vitro neural networks electrophysiology and non-invasive electroencephalogram.

}

7:30 pm – 9:30 pm

Student Symposium

Conveners: Cristofer Calvo, Cincinnati Children’s Hospital Medical Center, and Bretton Hale, Arkansas State University

Monday, June 8

}

8:00 am – 10:00 am

Frontiers in Single Cell Technologies

Conveners: Yue Yun, Corteva Agriscience, Bretton Hale, Arkansas State University, Brad Upham, Michigan State University, and Joshua Gasiorowski, Midwestern University

Speaker:
Christine Shulse, Lawrence Berkeley National Laboratory
Hillary Sullivan,Corteva Agrisciences

}

10:30 am – 12:30 pm

Applications of Omic’s Technologies

Conveners: Kolla Kristjansdottir, Midwestern University, and Addy Alt-Holland, Tufts University

In the last decade, innovations in state-of-the-art equipment, research methodologies and data analyses have revolutionized the omics fields. At the DNA and RNA levels, transcriptomics, genome wide associations, and most recently next-generation sequencing, have made genomic studies common even in small academic and agricultural labs. At the protein level, proteomics can be used to discover the identity of thousands of proteins and post-translational modifications in complex samples or to provide highly sensitive targeted quantification. At the cellular and systemic metabolism levels, metabolomics, is understudied partly because the metabolome cannot be determined from the genetic sequence of an organism. Comprehensive databases from experimental and clinical data are being compiled for use in toxicology, disease research, environmental analysis and agriculture. Collectively, the omics fields generate staggering amounts of data. The current challenge is to integrate this data with traditional cell and biochemical studies in order to provide a more complete and accurate picture of working biological systems. This session will discuss key applications, techniques and recent advances in genomics, proteomics and metabolomics.

Plant Biotechnology Post-Doctoral Oral Presentation Competition

Moderator: Carlos H. Garcia, CTC Genomics

To support the Society’s vision to encourage education and scientific informational exchange and recognize outstanding post docs, the Plant Biotechnology Section is pleased to announce the 2020 Post-Doctoral Oral Presentation Competition. Post-Doctoral Candidates wishing to participate in this competition should submit a copy of their abstract with its title and submission ID number to Carlos Hernandez-Garcia and check that option when they submit their abstract. Competition finalists will be selected based on the quality of the abstracts. The abstract should address the following: Background, Objectives, Methods, Results, Discussion and Conclusions. Where appropriate, the methods section should include a description of how reproducible results were ensured. The abstract must not include references. The abstract text must not exceed 1800 characters. A panel of judges will evaluate the presentations at the meeting. Criteria for the evaluation include experimental design, data analysis, proper interpretation of the results, originality of the study, technical difficulty, appearance and ability of the post-doctoral candidate to present it. Winners will be presented with a certificate and a cash award at the meeting. Please note that the DEADLINE to submit your abstract for the SIVB Oral Presentation Competition is January 31, 2020.

Plant Biotechnology Student Oral Presentation Competition

Moderator: Veena Veena, Donald Danforth Plant Science Center

To support the Society’s vision to encourage education and scientific informational exchange and recognize outstanding post docs, the Plant Biotechnology Section is pleased to announce the “Plant Biotechnology Student Oral Presentation Competition” at the 2020 World Congress on In Vitro Biology being held from June 6-10, 2020 in San Diego, California at the Town and Country San Diego. Students wishing to participate in this competition should submit a copy of their abstract with its title and submission ID number to Dr. Veena Veena and check that option when they submit their abstract. Student competition finalists will be selected based on the quality of the abstracts. The abstract should address the following: Background, Objectives, Methods, Results, Discussion and Conclusions. Where appropriate, the methods section should include a description of how reproducible results were ensured. The abstract must not include references. The abstract text must not exceed 1800 characters. A panel of judges will evaluate the presentations at the meeting. Criteria for the evaluation include experimental design, data analysis, proper interpretation of the results, originality of the study, technical difficulty, appearance and ability of the student candidate to present it. Winners will be presented with a certificate and a cash award at the meeting. Please note that the DEADLINE to submit your abstract for the SIVB Oral Presentation Competition is January 31, 2020.

 

}

12:30 pm – 1:30 pm

Student Networking Luncheon

Conveners: Cristofer Calvo, Cincinnati Children’s Hospital Medical Center, and Bretton Hale, Arkansas State University

}

1:30 pm – 2:30 pm

In Vitro Animal Cell Sciences Student and Post-Doctoral Oral Presentation Competition

Moderators: Kolla Kristjansdottir, Midwestern University, and Addy Alt-Holland, Tufts University

 

The In Vitro Animal Cell Sciences Section (IVACS) of the Society for In Vitro Biology is pleased to announce the 2020 Student and Post-Doctoral Oral Presentation Competition during the World Congress on In Vitro Biology. This competition encourages the exchange of scientific information between the presenters, attendees and judges, and provides an invaluable opportunity for students and post-docs to enhance their presentation delivery and public speaking skills. Students and post-docs who wish to participate in this competition should check that option when they submit their abstract to the 2020 SIVB’s World Congress. In addition, applicants should e-mail a copy of their complete abstract and submission ID number to the session moderators, Dr. Addy Alt-Holland (addy.alt_holland@tufts.edu) and Dr. Kolla Kristjansdottir (kkrist@midwestern.edu). The top three finalists will be selected for the competition based on the quality of their abstracts, as well as the merit of their research and scientific findings. The text of the abstract should include the following sections: Background, Objectives, Methods, Results, Discussion and Conclusions. Where appropriate, the Methods section should include relevant statistical analysis. The abstract text should not exceed 1800 characters, and it should not include references. During the Oral Presentation Competition session, a panel of expert judges will grade the presentations. Evaluation criteria will include: experimental design, data analysis, proper interpretation of the results, originality of the study, technical difficulty, professionalism and ability of the finalist to explain the research and answer questions, and importantly, adherence to the allocated time for the presentation. The Student and Post-Doctoral Oral Presentation Competition session serves to recognize and reward the research and achievements of outstanding students and talented post-docs. Therefore, the three finalists will be presented with a certificate and a cash award during the World Congress. The DEADLINE for abstract submission for the Student and Post-Doctoral Oral Presentation Competition is January 31, 2020.

 

}

3:30 pm – 5:00 pm

Building Partnerships and Resources to Address Transformation Bottlenecks

Conveners: Mary Ann Saltarikos, Bayer U.S. – Crop Science, and Joyce Van Eck, Boyce Thompson Institute

Speakers:
Building Partnerships and Approaches for Overcoming Bottlenecks that Prevent Efficient Genetic Engineering and Gene Editing
Mary Ann Saltarikos, Bayer U.S. – Crop Science
Challenges that Hinder Progress of Genetic Engineering and Gene Editing
Joyce Van Eck, Boyce Thompson Institute

Panelists:
Raj Deepika Chauhan, Pairwise
William Gordon-Kamm, Corteva Agriscience
Javier Narvaez
, Cibus US LLC
Mary Ann Saltarikos, Bayer U.S. – Crop Science
Nigel Taylor, Donald Danforth Plant Science Center
Joyce Van Eck, Boyce Thompson Institute
Kan Wang, Iowa State University

 

Bottlenecks that thwart achieving efficient genetic engineering and gene editing approaches in plants can come at various stages including plant regeneration, transformation, and recovery of quality (low copy number, desirable phenotype) modified lines.  With the advent of gene editing technologies, tailor-made genetic modifications to help advance crop improvement can be realized and has resulted in a demand more than ever before for reliable, robust genetic engineering pipelines.  Efforts to develop these pipelines are underway by researchers in both the public and private sectors; however, progress is often slowed due to several factors such as access to germplasm, limited funding, and lack of resources that include infrastructure and experienced personnel.  The purpose of this session is to explore possibilities for building connections within and between public and private research organizations.  Through two presentations that will present overviews of the issues, a panel discussion, and audience participation we intend to establish a foundation that will help us move forward by identifying where the greatest needs are and how we can collaborate as a community to address them.

Digital Agriculture – Sensors, Machine Learning and Image Analysis

Conveners: Randall P. Niedz, USDA, and Kan Wang, Iowa State University

Speakers:
Precision  Agriculture Sensors
Liang Dong, Iowa State University
Image Analysis and Machine Learning
Beth Cimini, Broad Institute of MIT and Harvard
In Vitro Data Collection Using Image Analysis and Machine Learning
Randall Niedz, U.S. Horticultural Research Laboratory, USDA

A large number of devices/sensors exist that provide a quantitative and objective representation of the plant, in planta and ex planta, and its environment. Many of these devices elucidate properties, often in real-time, either not visible to the human eye or otherwise impossible or difficult to quantify without these devices. These devices typically generate large amounts of data, and software is required to collect, organize, and analyze these large data sets. The combination of these devices and software is redefining what is meant by “field observable” properties. The results include precision agriculture where all aspects of crop production are controlled more accurately, and agricultural research where these measures provide a new level of detail that is furthering our understanding of the genetic x environment interactions and the complexity that characterizes biological systems. The technological goal is the precise monitoring, quantification, and control of all aspects of plant growth and development. The session will discuss sensor technology, including microsensors for real-time individual plant phenotyping, the principles and advances in image analysis and machine learning, and the collection and use of data obtained by machine learning image analysis in classical and DOE plant tissue culture experiments.

MicroRNA and Cellular Dysfunction

Conveners:  Jeff Kwak, University of Colorado, Denver, and Michael Fay, Midwestern University

Speakers:
Role of miR-145 in SMC Differentiation
Yi-Ting Yeh, University of California San Diego
MicroRNAs in Hematopoeitic Development and Cancer
Dinesh Rao, UCLA Health
The Role of Extracellular Vesicle MiRNAs in Promoting Breast Cancer Stemness
Shizhen (Emily) Wang, University of California, San Diego

MicroRNAs in Hematopoeitic Development and Cancer
Dinesh Rao, UCLA Health

MicroRNAs (miRNAs) are small (20-25 nt) non-coding RNAs that block gene expression at the post-transcriptional level. It is estimated that the expression of over 50% of mammalian protein coding genes are regulated by miRNAs, and a single miRNA may regulate expression of hundreds of mRNAs. Since the discovery of miRNAs, there has been great interest in determining the roles of miRNAs in regard to cellular function and dysfunction. This symposium will focus on the roles of non-coding RNAs with an emphasis on cancer and differentiation.

Non-competitive Student Oral Presentations

Moderators: Cristofer Calvo, Cincinnati Children’s Hospital Medical Center, and Bretton Hale, Arkansas State University

 

}

5:00 pm - 6:00 pm

Advances in Plant, Cell, Tissue, and Organ Culture Contributed Paper Session

Moderator: Nagesh Sardesai, Corteva Agriscience

IVACS Contributed Paper Session

Moderator: TBD

Plant Contributed Paper Session

Moderator: Ming Cheng, Pairwise

}

8:30 pm - 10:00 pm

Transformation of Maize and Other Cereals Using Morphogenic Genes: An Update and Practical Tips

Conveners: Bill Gordon-Kamm, Corteva Agriscience, Kan Wang, Iowa State University, and Ajith Anand, Corteva Agriscience

Panelists:
Bill Gordon-Kamm, Corteva Agriscience
Kan Wang, Iowa State University
Ajith Anand, Corteva Agriscience

Plant morphogenic genes can be used to improve genetic transformation of recalcitrant maize inbred genotypes as well as other cereal crops.  In this workshop, we intend to share our protocol details and tips for maize inbred transformation.  We will show video to demonstrate the step-by-step protocol for Agrobacterium-mediated immature embryo transformation.  This procedure has been successfully reproduced by researchers with minimum maize transformation training.  Following the video (with discussion), a presentation on maize leaf transformation will also be used to contrast differences between the two protocols.  Finally, we’d like to hear from researchers who have attempted using the morphogenic genes, emphasizing both what worked well and where there were problems, providing an opportunity to discuss trouble-shooting the process.   

Tuesday, June 9

}

8:00 am – 10:00 am

Bioethics and Public Policy for Benefits and Concerns in Plants and Animals: Genome Editing to the 3Rs

Conveners: Albert Kausch, University of Rhode Island, Kenneth Kandaras, International Foundation for Ethical Research, and Addy Alt-Holland, Tufts University

Speakers:
David Resnik, National Institute of Environmental Health Sciences

 

}

10:30 am – 12:30 pm

Emulating Human Liver in Preclinical Research and Regenerative Medicine

Conveners: Seyoum Ayehunie, MatTek Corporation, and John W. Harbell, JHarbell Consulting

Speakers:
Mechanistic Insight and Prediction of Drug-induced Organ Injury for Humans
Alison EM Vickers, Human Translational Models, LLC
Modeling Non-alcoholic Steatohepatitis (NASH) Using 3D Bioprinted Human Liver Tissue
Tatiana Kisseleva, University of California, San Diego
An In Vitro Model of Human Fatty Liver Disease
Aras Mattis, University of California, San Francisco

 

A chronic problem in pharmaceutical development has been the frequency that candidate compounds fail in clinical trials due to unexpected hepatic toxicity. In these cases, the preclinical testing in animal models had failed to identify a selective human toxicity. In addition, there is a growing need to model human liver damage such as non-alcoholic steatohepatitis (fatty liver disease) which is now driving a majority of liver transplantation cases. In vitro liver tissue models are gaining importance for safety and efficacy prediction of pharmacological research and disease modeling. Obtaining primary liver cells on a continuous basis is difficult at best. To overcome these limitations, emerging technologies provide alternative liver cell source including inducible pluripotent stem cells (iPSCs) for in vitro predictive models for liver physiology and functionality.  Such models would allow for the study of hepatotoxicity, xenobiotic metabolism and selective organ degeneration. The challenge has been compounded by the limited availability of donor organs, multiple cells types to address within the liver and the rapid reduction in xenobiotic metabolic competence (p450 activity) with time in traditional culture. To address these challenges, 3D culture systems have been developed to allow maintenance of normal cell shape and interaction. In some cases, multiple cell types can be incorporated. These and other features of the models allow the maintenance of some degree of p450 activity. This symposium will feature three approaches to address the promise and challenges in the development of physiologically functional 3D liver cell cultures. These presentations will include liver slide culture from mature liver, bioprinting of 3D structures from component cell types and development of iPSC-derived hepatocytes for research and regenerative medicine.
 

Plant Genome Engineering – From Lab to Consumers

Conveners: Harold Trick, Kansas State University, and Jyoti Rout, Intrexon Corporation

Speakers:
Powering Innovation in Food for a Healthier Planet and People
Sekhar Boddupalli, Intrexon Corp
Synthetic Apomixis for Hybrid Crop Propagation
Imtiyaz Khanday, University of California, Davis
Consumers Attitudes Towards Gene Editing
Brandon R. McFadden, University of Delaware

Plant Memory: The Importance of Assessing Culture Carry Over Effects During Micropropagation Protocol Development

Conveners: Michael E. Kane, University of Florida, and Benjamin Hughes

}

3:30 pm – 5:00 pm

Responsible Plant Genome Engineering: Science, Stewardship, and Social Licenses

Conveners: Wayne Parrott, University of Georgia, and Albert Kausch, University of Rhode Island

Speakers:
Background & Precedents
Wayne Parrott, University of Georgia
Social Contract & Self-policing Scientists
Albert Kausch, University of Rhode Island
Restricting Access Across the Board
Veena Veena, Donald Danforth Center Plant Science Center
Escape from the Lab
Joyce Van Eck, Boyce Thompson Institute
Escape from the Greenhouse
Albert Kausch, University of Rhode Island
Escape During Shipping
Kan Wang, Iowa State University
Mixups During Storage
Heidi Kaeppler, University of Wisconsin
Escape from Field Trials
Heidi Kaeppler, University of Wisconsin

Trust between science and society is essential for society to function. At a time when trust in science is at an all-time low, it is particularly important that scientists show they are responsible citizens and stewards of their plant research materials. Genetic modification remains a controversial topic, more so because it is remarkably easy for transgenes or edited events to ‘escape,’ and the associated press coverage undermines public trust and support for science. Furthermore, they can result in severe disruptions to trade, resulting in billions of dollars in economic losses for the people involved, not to mention financial liability for the scientist and the institution for which they work. Recent breakthroughs in transgenic technology, combined with advances in genome editing, mean that transgenes and edited plants will be more widely available and in more labs than ever before. At the same time, major regulatory rollbacks proposed by USDA will contribute to the perception that relaxed handling of transgenes is permissible, just as improved sequencing technology is making the detection of stray transgenes or genome edits easier than ever before. Therefore, within the context of the social contract that must exist between science and society, this session will highlight common practices that permit event escape, and describe simple, practical solutions for effective event containment.

 Imaging Analytics, Artificial Intelligence and Robotics in Tissue Culture and Transformation

Convener:  Yurong Chen, Bayer Crop Science

Speakers:
Recent Developments in Imaging Analytics, Artificial Intelligence and Robotics
Balathasan Giritharan, Bayer Crop Science
Phenomic System for Imaging and Quantification of In Vitro Plant Regeneration and Transformation
Steven Strauss, Oregon State University
RoboCut-smart Propagation, Better Plants
Max Welikow, BOCK Bio Science GmbH

Increasing shooting or transformation frequency through cell biology manipulation, improving consistency and reducing the labor costs are some of the main approaches to improve the tissue culture and transformation production efficiency. Most tissue culture and transformation systems are long and have multiple-stage process involving medium changes and manual transfers or manual cuttings in some applications. Imaging analytics and artificial intelligence can be applied to identify and quantify desirable cultures. Robotics are then used to cut or transfer the desirable cultures to fresh medium. This will help improve consistency, reduce cost, and increase production efficiency. With recent advancements in image sensing and breakthroughs in training deep architectures of neural networks, performance of machine learning has moved closer towards artificial intelligence. Particularly, deep learning has been successfully applied to complex image classification and retrieval tasks. This symposium will focus on the current status and future opportunities of leveraging imaging analytics, artificial intelligence and robotics to improve tissue culture and transformation.

Microbiome in Mammalian Health

Conveners:  Debora Esposito, North Carolina State University, and Mae Ciancio, Midwestern University

Speakers:
Ex Vivo Fecal Fermentations: Biotransformation of Dietary Compounds to Screen for Bioactive Microbial Metabolites
Andrew Neilson, North Carolina State University
R. William DePaolo, University of  Washington

Wednesday, June 10

}

8:00 am – 10:00 am

Current Perspectives on Cannabis and Cannabinoids

Conveners: Max Jones, University of Guelph, Katya Boudko, Canopy Growth Corporation, Jeffery W. Adelberg, Clemson University, and Evan Hill, University of Michigan

Speakers:
John W. Wiley, University of Michigan
Oliver Kayser, Technische Universität Dortmund
Brian Reid
, Canopy Growth Corporation

Cannabis sativa L. is one of the first medicines known to man. However, evidence-based information on the role of cannabis constituents for human health and well-being has been hampered in modern time by strict prohibition policies. Many of these restrictions are rapidly disappearing in nations across the globe, opening a path for new approaches to bring forward information and products. Our speakers in this session on Current Perspectives on Cannabis and Cannabinoids cut across disciplines of In Vitro Biology to show by example, some of the varied approaches to develop a better understanding of this complex plant. Phytocannabinoids, terpenes, flavins, and other compounds from the cannabis plant are characterized to develop a better understanding of the broad landscape of medicinal, neutraceutical and recreational effects. Phytotherapy and rational clinical research may be supported by bioengineered cannabis or micro-organisms delivering specific compounds, and of special interest, compounds rare in planta. With the availability of high-quality phytochemicals, specific effects on the human gut and their perception by the brain are now elucidated. Transdisciplinary research is well demonstrated in these pursuits.

}

10:30 am – 12:30 pm

Exploring Microbiomes: Application to Humans and Agriculture

Conveners: Rodrigo Sarria, Agbiome and Kristina Martinez-Guryn, Midwestern University

Speakers:
Jack Gilbert, University of California, San Diego
Joseph F. Pierre, University of Tennessee

The session “Exploring Microbiomes: Application to Humans and Agriculture” is a joint session encompassing both plant and animal researchers and will highlight the importance of applying microbiome research to human health and agriculture. The goal of this session is to build an appreciation for the integral role of environmental and host microorganisms in facilitating physiological processes in humans and plants. The invited speakers are experts that cover a wide range of microbiome research and areas of application including the plant microbiome (Dr. Ben Holt, Core Program Leader – AgBiome Inc.), the fungal microbiome or mycobiome (Dr. Joseph F. Pierre, Assistant Professor – University of Tennessee Health Science Center), and microbial ecology (Jack Gilbert, Professor, University of California San Diego, co-founder of the Earth Microbiome Project and American Gut Project), thus offering comprehensive presentations on microbial ecology and important host-microbe interactions that contribute to health and disease.

Beyond KOs: Emerging Genome Editing Technologies

Conveners: Mary Ann Saltarikos, Bayer U. S. – CropScience, and Aaron Hummel, Pairwise

Speakers:
Investigating the Chemical and Cellular Mechanisms of Base Editing
Alexis Komor, University of California, Davis
Luca Comai,
University of California, Davis
C. Neal Stewart, Jr
., University of Tennessee

CRISPR has enabled tremendous progress in basic and applied biology across kingdoms. However, most studies are still using nuclease-mediated, loss of function strategies, focused on single genes in the nuclear genome. In anticipation of the future of the genome editing field, this session will examine new technologies enabling gain of function studies, targets and challenges for editing outside the nuclear genome, and progress toward upscaling from gene to genome editing. Topics will include base editing tools, plastid genome editing, and structural remodeling of the nuclear genome.

 

Advanced Synthetic Biology Tools for Plant Biotechnology

Conveners: Prakash Kumar, National University of Singapore, Alessandro Occhialini, University of Tennessee, and Lori Marcum, Corteva Agriscience

Speakers:
Scott Lenaghan, University of Tennessee
Cheryl Kerfeld, Lawrence Berkeley National Lab and Michigan State University
A Novel Plant Cell-free Synthetic Biology Platform and Its Applications
Krishna Madduri
, Corteva Agriscience

Synthetic biology is a rapidly growing interdisciplinary area that applies engineering principles of design-build-test cycles to synthesize entire new biological organisms or parts. The ability to introduce complex multi-gene pathways into organisms has found many applications in plant biotechnology to generate novel, favorable phenotypes and for production of valuable molecules in plants (vaccines and biofuels are only a few examples). This session will focus on advanced synthetic biology tools for precise engineering of plant cells and organelles. The possibility to perform analysis on single cells and subcellular compartments like plastids has considerably expanded the toolbox of plant synthetic biology. The session will also focus on progress made in design of synthetic plastid genomes and the prospect of assembling new proteinaceous micro-compartments for precise metabolic engineering.

Share this page