Tuesday, June 11

Daily Program at-a-Glance

Time Event Location
7:00 am – 5:00 pm Registration Grand Salons A-D Foyer
10:00 am – 3:30 pm Exhibits and Posters Grand Salons A-E
Morning 7:00 am – 12:30 pm
7:00 am – 8:00 am In Vitro – Animal Editorial Board Meeting Meeting Room 10
Student Affairs Breakfast Meeting Rooms 8&9
8:00 am – 10:00 am Maximizing Gene Editing Target Specificity Grand Salon F
10:00 am – 10:30 am Coffee Break Grand Salons A-E
Constitution and Bylaws Committee Meeting Meeting Room 10
Education Committee Meeting Meeting Room 11
10:30 am Closing of The Big Guava Silent Auction Grand Salons A-E
10:30 am – 12:30 pm Biotechnology and Its Importance for the Citrus Industry Grand Salon F
Cellular Agriculture and the Use of Cell Lines for Meat Production            Meeting Room 12
Recalcitrance in Micropropagation, Regeneration and Transformation Grand Salons G-J
Afternoon 12:30 pm – 5:30 pm
12:30 Announcement of The Big Guava Silent Auction Winners Grand Salons A-D Foyer
12:30 pm – 1:30 pm 2020 Program Planning Committee Meeting Meeting Room 10
Development Committee Meeting Meeting Room 11
1:30 pm – 2:30 pm Interactive Poster Sessions
In Vitro Animal Cell Sciences
Micropropagation
Genome Editing
Grand Salons A-E
2:30 pm – 3:30 pm Poster Session
Odd Poster Authors will be present
Grand Salons A-E
3:30 pm – 4:00 pm Poster Breakdown and Removal Grand Salons A-E
3:30 pm – 5:00 pm 3D Cell Cultures in Cancer Research: Modeling In Vivo Tumors Meeting Room 12
Regulation of Morphogenesis Grand Salon F
In Vitro Ecophysiology Grand Salons G-J
 5:00 pm – 5:30 pm SIVB Business Meeting
(All Members Are Urged to Attend)
Student Award Presentations
Meeting Rooms 8&9
 5:45 pm – 9:00 pm Its a Whale of a Time: An Evening at the Florida Aquarium
Admittance by Advance Ticket Holders Only
Meet buses at 5:40 pm
 Off Property

Tuesday, June 11

7:00 am – 5:00 pm Registration Grand Salons A-D Foyer

MAXIMIZING GENE EDITING TARGET SPECIFICITY

Conveners:    Mary Ann Saltarikos, Bayer U. S. – Crop Science, and Michael J. Fay, Midwestern University

8:00 am – 10:00 am Plenary Symposium Grand Salon F

The CRISPR-Cas9 system has become an important method for genome editing in both plant and animal cells providing unprecedented opportunities for crop improvements and therapeutic applications.  A possible important hurdle of this technology is the extent of its off-target activity and despite the importance of these off-target cleavage events, we still lack a full understanding of the biophysical basis for the specificity of CRISPR-Cas enzymes. The speakers in this plenary session will discuss recent developments regarding improvements in the specificity of CRISPR/Cas9 and off-target detection methods.

8:00

Introduction (M. A. Saltarikos and M. J. Fay)

8:05 PS-7 Improving CRISPR-Cas9 Activity and Specificity by Chemical Modification of the sgRNA Backbone
Dan Ryan, Agilent Technologies
8:40 PS-8 Activity and Specificity of CRISPR-Cas9 and Cas12a Systems in Plant Genome Editing
Yiping Qi, University of Maryland – College Park
9:15 PS-9 Systematic Evaluation of CRISPR-Cas9 Specificity and Its Relevance in Crop Improvement
Sandeep Kumar, Corteva AgriscienceTM, Agriculture Division of DowDuPontTM
9:50 Discussion


10:00 am – 10:30 pm Coffee Break Grand Salons A-E

BIOTECHNOLOGY AND ITS IMPORTANCE FOR THE CITRUS INDUSTRY

Conveners:   Ahmad Omar, University of Florida, and Randall P. Niedz, USDA-US Horticultural Research Laboratory

10:30 am – 12:30 pm Plant Symposium Grand Salon F

Citrus is one of the most important commercial and nutritional fruit crops in the world, but faces a number of challenges including concerns about the environmental impacts of production, farm labor shortages, diverse and changing consumer demands, and the global movement of insect pests and diseases.  Improved cultivars can address each of these challenges. However, the conventional breeding of citrus is difficult because of the combined effects of facultative apomixis, long juvenile periods, high heterozygosity, sexual incompatibility, pollen/ovule sterility, and little knowledge of the inheritance of horticultural traits. Biotechnology provides approaches that can potentially overcome many of these biological limitations, as well as address the challenges, particularly those related to serious pests and diseases. Huanglongbing (HLB), also known as citrus greening disease, is the most serious citrus disease worldwide.  HLB is a significant threat to global citrus production because it is found in many of the major citrus growing areas. Producing disease/(HLB)-resistant genotypes using biotechnology is expected to result in cultivars with good field performance that produce fruit economically and with acceptable consumer traits. A range of plant biotechnological techniques and approaches such as cell and tissue culture, genetic engineering, molecular genetics, and genome editing will result in long-term and rapid improvements.

10:30 Introduction (R. P. Niedz and A. Omar)
10:35 P-19 Aiming at Huanglongbing, and the Search for Genetic Ammunition
Fred Gmitter, University of Florida
11:00 P-20 Application of Biotechnology to Develop Huanglongbing Resistant /Tolerant Citrus Scions
Ed Stover, USDA, US Horticultural Research Laboratory
11:25 P-21 Recombinase Technology for Transgene Manipulation
James Thomson, USDA, Crop Improvement and Genetics Research
11:50 P-22 Generating Disease Resistant Citrus Varieties Using the CRISPR Technology
Nian Wang, University of Florida
12:15  Discussion



CELLULAR AGRICULTURE AND THE USE OF CELL LINES FOR MEAT PRODUCTION

Conveners:    Lucy E. J. Lee, University of the Fraser Valley, and Vivian R. Dayeh, University of Waterloo

10:30 am – 12:30 pm Animal Symposium Meeting Room 12

As the demand for clean meat increases, scientists are looking for ethically sourced, contaminant-free meats. Invitromaticists (cell culture researchers) have begun to mass produce the meat invitrome, that is, fibroblasts, muscle and fat cells, that are the cellular components of meat, under in vitro conditions. Additionally, molecular biologists and tissue bioengineers are collaborating with the invitromaticians to bring muscle mass for bioreactor production and scale-up. This revolution in meat production without the need to continuously kill animals is spawning new biotech companies specializing in the production of select meat invitromes, ie. using continuously growing cell lines derived from the species of interest, including fish, birds and mammals, for clean meat production. This session will provide a forum for discussion and knowledge sharing of the current state in cellular agriculture or meat invitromatics.

10:30 Introduction (L. E. J. Lee and V. R. Dayeh)
10:35 A-11 The Benefits of Cell-based Seafood
Mike Selden, Finless Foods
11:00 A-12 Breaking Down a Fish, the Cellular Way!
Jennie Tung, Finless Foods
11:25 A-13 Automation in Cell-based Meat Production
Nicholas Legendre, New Age Meat
11:50 A-14 Creating Cellular Agriculture as an Interdiscipline
Kate Krueger, New Harvest
12:15 Discussion


RECALCITRANCE IN MICROPROPAGATION, REGENERATION AND TRANSFORMATION

Conveners:  Jeffrey Adelberg, Clemson University, and Theodore Klein, Meristematic Inc

10:30 am – 12:30 pm Plant Symposium Grand Salons G-J

Overcoming recalcitrance and expressing competence has been a vexing problem among the community of biotechnologists involved with regeneration and genetic transformation of non-model crop species. For an even longer period of time, recalcitrant and permissive genotypes have made commercial micropropagation of some varieties erratic, when other apparently similar plants are reliably propagated via tissue culture. Recalcitrance and competence are phenotypes, with their expression determined by genetic and environmental factors. Therefore, both environmental and genetic factors need to be considered in efforts to overcome recalcitrance. Our invited speakers will share perspective along an array of basic approaches and practical experiences with recalcitrance. We draw from both academic and commercial practitioners to share their best ideas in understanding and manipulating recalcitrance.

10:30 Introduction (J. Adelberg and T. Klein)
10:35 P-23 Deciphering the Genetic Mechanisms Underlying Somatic Embryogenesis in Plant Regeneration of Maize
Heidi Kaeppler, University of Wisconsin
11:15 P-24 Re-conceptualizing the In Vitro Production Pipeline for Recalcitrant Perennial Crops
Amit Dhingra, University of Washington
11:35 P-25 A Rapid Sunflower Transformation Method: Applying New Tools to an Old Method
George Hoerster, Corteva Agriscience
11:55 P-26 Dynamic Transcriptional Regulation of DNA Methylation and Transcription Factors Control the Transition from Non-embryogenic Callus to Embryogenic Callus in Upland Cotton
Christopher Saski, Clemson University
12:15 Discussion


IN VITRO ANIMAL CELL SCIENCES 

Moderator:             Kolla Kristjansdottir, Midwestern University

1:30 pm – 2:30 pm Animal Interactive Poster Session Grand Salons A-E
A-2000 Efficient Serum-free Cryopreservation of Cell Lines at -20oC and -80oC
Cynthia L. Goodman, USDA/ARS Biological Control of Insects Research Laboratory, Xu Han, Yuping Huang, Claire Dubos, Joseph Ringbauer, Jr., Megan M. Augustin, and David Stanley
A-2001 Evaluating Potential Genotoxicity of Imidacloprid with Fish Cell Lines
Brenna N. Hay, University of the Fraser Valley, and Lucy E. J. Lee
A-2002 The Role of Gut Microbes in Regulating the Constitutive Androstane Receptor and Metabolism.
Elesa Poteres, Midwestern University, Iara Ibay, Lauren Alt, Kelly Keeler, Matt Pytynia, Allison Isabelli, and Kristina Martinez-Guryn
A-2003 Bioavailability of Artemisinin from Artemisia annua: Gender Differences in Absorption Distribution Metabolism and Excretion and Inhibition of Hepatic Cytochrome P450 Enzymes by A. annua Extracts and Phytochemicals
Matthew Desrosiers, Worcester Polytechnic Institute, Alexis Mitteleman, and Pamela J. Weathers
A-2004 Fabricating Three-dimensional Plant-based Biomaterials to Support Mammalian Cell Growth and Tissue Formation
C. J. Niemiec, Midwestern University, and J. Z. Gasiorowski

 

MICROPROPAGATION

Moderator:     David May, University of Florida

1:30 pm – 2:30 pm Plant Interactive Poster Session Grand Salons A-E
P-2006 Developing a Tissue Culture Protocol for the Propagation of Reduced Juvenility Transgenic ‘Carrizo’ Citrange Rootstock
K. Aljasim, University of Florida, M. Dutt, A. A. Omar, and J. W. Grosser
P-2007 In Vitro Propagation of Peach Fruit Tree Is Not an Easy Job.
Bipul Kumar Biswas, Fort Valley State University, Sylvia Chirchir, Nabin P. Sedhain, and Monice McKinney
P-2008 Cannabis sativa Synthetic Seed Cold Preservation and Regeneration
Angelo Alvarez, Knockout Genetics, Savanah St. Clair, and Norman Senn
P-2009 Establishment of an Improved Micropropagation Protocol for an Industrially Important Plant, Hedychium coronarium and Assessment of Genetic and Biochemical Stability
Soumendra K. Naik, Ravenshaw University, Shashikanta Behera, Pradeep K. Kamila, Kedar K. Rout, Durga P. Barik, and Pratap C. Panda
P-2010 Silicon Nanoparticles Mitigate the Adverse Effect of Drought Induced by Polyethylene Glycol of In Vitro Banana Shoots
Lamiaa M. Mahmoud, Mansoura University, M. E. EL-Kady, M. S. Elboray, A. N. Shalan, Y. M. Shabana, and Jude W. Grosser
P-2011 Sustainable Commercial Black Pepper Production Through Micropropagation and Organic Agriculture
Virendra M. Verma, Micronesia Plant Propagation Research Center
P-2012 Benefits of Ex Vitro Rooting in Cannabis sativa
Norman Senn, Knockout Genetics, Savanah St. Clair, and Angelo Alvarez

 

GENOME EDITING

Moderator:     Raj Deepika Chauhan, Pairwise

1:30 pm – 2:30 pm Plant Interactive Poster Session Grand Salons A-E
P-2020 Plant Genome Editing and DNA Repair in the Context of DNA Methylation and Chromatin Dynamics
Trevor J. Weiss, University of Minnesota, Peter Crisp, Nathan Springer, and Feng Zhang
P-2021 Genome Editing of the NRT1.1 Gene Family in Maize
Brian H. Rhodes, University of Illinois, Stephen Jinga, and Stephen Moose
P-2022 Genome Editing of Tomato with CRISPR/Cas 9 Targeting Fruit Softening Genes
Murray Boase, The New Zealand Institute for Plant and Food Research Limited, Heather Tiffin, Huaibi Zhang, Donald Hunter, Zoe Erridge, Lei Wang, Sarina Mandhandhar, Ian King, Nathanael Napier, David Brummell, and Robert Schaffer
P-2023 Wheat Genome Engineering via CRISPR/Cas9 to Generate Virus Resistance
Monica Navia-Urrutia, Kansas State University, Jessica L. Rupp, John P. Fellers, and Harold N. Trick
P-2024 Building a Suite of CRISPR/Cas9 Tools for Efficient Switchgrass Gene Editing
Eudald Illa-Berenguer, University of Georgia, Peter R. LaFayette, Gary A. Orr, and Wayne Parrott
P-2025 Optimization and Application of Homology Independent Targeted Integration in Plants
Andika Gunadi, The Ohio State University, Nuananong Orchard, Feng Qu, and John J. Finer
P-2026 Precise Genome Editing in Plants Using Homology Directed Repair
M. Becker, Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), and G. Hensel

 

Tuesday, June 11
Odd Poster Authors will be present
2:30 pm – 3:30 pm

 

3D CELL CULTURES IN CANCER RESEARCH: MODELING IN VIVO TUMORS

 

Conveners:     Addy Alt-Holland, Tufts University and Brad L. Upham, Michigan State University

3:30 pm – 5:00 pm Animal Symposium Meeting Room 12

Cells grown in traditional monolayer cultures allow scientists not only to study many fundamental biological processes, such as cell adhesion and migration, growth, metabolism and response to different therapeutic agents, but also to decipher and interrogate molecular mechanisms that are involved in these processes. Unlike monolayer cultures, more complex, in vitro 3D models, including spheroids, tumoroids and bioengineered tissues, can better provide cancer cells with physiologically relevant microenvironments, tissue architecture and molecular cues for the development of in vivo-like tumors. These models allow the investigation of complex processes associated with cancer progression, invasion and metastasis, as well as the identification of key signaling pathways that drive these processes. Furthermore, the use of 3D systems in cancer research is critical for the development of new anti-cancer drugs, prediction and validation of their efficacy, improved diagnostic strategies, and advancement of personalized and patient-tailored cancer treatments. This session will highlight the development and applications of 3D tissue models in cancer research.

3:30 Introduction (A. Alt-Holland and B. L. Upham)
3:35  A-15 3D Fibrous Scaffold
Subhra Mohapatra, University of South Florida
4:15  A-16 From Bioengineered Cancer Tissues to Human Clinical Studies – Translational Research in Skin Biology
Addy Alt-Holland, Tufts University
4:55 Discussion



REGULATION OF MORPHOGENESIS

Conveners:    Prakash Venglat, University of Saskatchewan, and William Gordon-Kamm, CORTEVA AgriscienceTM, Agriculture Division of DowDuPontTM

3:30 pm – 5:00 pm Plant Symposium Grand Salon F

Stem cells of plants are wired differently to differentiation programs compared to animals. This provides for an iterative process that elaborates the plant body over time and is developmentally and environmentally regulated. In addition, the organized and well-integrated development of embryos, shoots, and roots can be recapitulated in vitro using explants derived from different tissue types. This highlights the inherent plasticity of the plant cell to rewire their morphogenetic states and recover their regenerative potential. Progress in understanding the underlying molecular genetic control of meristem and embryo ontogeny has been spectacular and provides ample fuel to feed new strategies in transformation research. A number of morphogenic genes have been used to improve plant regeneration and/or transformation over the last 20+ years, but recently there has been renewed interest in these methods. Use of morphogenic genes such as WUS and BBM provides a good example of an unfolding story that increasingly facilitates transformation in cereals such as maize, sorghum and wheat. This session will highlight new developments in plant morphogenic gene technology and perspectives on new opportunities.

3:30 Introduction (P. Venglat and W. Gordon-Kamm)
3:35 P-27 Why Transgenics Are Important in Understand Meristem Regulation
David Jackson, Cold Spring Harbor Laboratory
3:55 P-28 miRNA Regulation of Early Embryogenesis in Arabidopsis thaliana
Stewart Gillmor, Langebio, CINVESTAV-IPN
4:15 P-29 Improved Maize Transformation Using Babyboom and Wuschel
Ning Wang, 
CORTEVA AgriscienceTM, Agriculture Division of DowDuPontTM
4:35 P-30 Gene Editing Through De Novo Induction of Meristems on Seedlings
Ryan Nasti, University of Minnesota
4:55 Discussion


IN VITRO ECOPHYSIOLOGY

Conveners:     Valerie Pence, Cincinnati Zoo & Botanical Garden, and Ben Hughes

3:30 pm – 5:00 pm Plant Symposium Grand Salons G-J

Plants have developed physiological adaptations to survive a wide range of environmental conditions in their native ecosystems such as photoperiod, water relations, nutrient availability, and soil microbiota. These responses are most commonly observed in vitro through differential growth between species, but also within a species, when grown in a common culture environment. How to address ecophysiological responses in vitro should be considered when producing plants in both the nursery industry and for conservation efforts. The aim of this session is to link ex vitro ecophysiological responses to those in vitro, provide examples using plants from different environments, and demonstrate practical applications for in vitro plant culture.

3:30 Introduction (V. Pence and B. Hughes)
3:35 P-31 Symbiotic Seed Germination of Orchids: Techniques and Perspectives
Larry Zettler, Illinois College
4:00 P-32 A Multi-species Evaluation of Factors Affecting Growth and Phenotype In Vitro
Valerie Pence
, Cincinnati Zoo & Botanical Garden
4:25 P-33 Lessons Learned from a Commercial Lab
Calvin Anderson,
Better Blooming Orchids
4:50 Discussion

 

5:00 pm – 5:30 pm SIVB Business Meeting
(All Members Are Urged to Attend)
Student Award Presentations
Meeting Rooms 8-9

5:45 pm – 9:00 pm Tuesday Evening at the Florida Aquarium
Admittance by Advance Ticket Holders Only
Off Property
Contact the Society for In Vitro Biology
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