Sunday, June 9

Daily Program-at-a-Glance

Time Event Location
7:00 am – 5:30 pm Registration Grand Salons A-D Foyer
10:00 am – 2:00 pm
5:30 pm – 7:30 pm
Exhibits and Posters Grand Salons A-E
Morning 7:00 am – 12:30 pm
7:00 am – 8:00 am In Vitro Animal Cell Sciences Program Committee Meeting Meeting Room 12
8:00 am – 10:00 am Frontiers of In Vitro and Synthetic Biology Grand Salon F
10:00 am – 10:30 am Coffee Break Grand Salons A-E
Nominating Committee Meeting Meeting Room 10
Public Policy Committee Meeting Meeting Room 11
10:30 am – 12:30 pm Biological Sensors: Organoids to Organisms for Answering Medicinal, Agricultural and Environmental Questions      Meeting Room 12
Engineering Stress Tolerance in Crop Plants Grand Salons G-J
Micropropagation Best Practices Grand Salon F
11:00 am – 12:00 pm SIVB/IAPB/Springer Business Meeting Meeting Room 10
Afternoon 12:30 pm – 5:30 pm
12:30 pm – 1:30 pm Exhibitors Refreshment Break Grand Salons A-E
Membership Committee Meeting Meeting Room 11
Micropropagation Best Practices Panel     Meeting Rooms 8&9
1:30 pm – 2:45 pm In Vitro Biotechnology Grand Salon F
1:30 pm – 3:00 pm Metabolic Engineering for Value Added Plant Products and Biofuels Grand Salons G-J
Stem Cell Differentiation in Human Models Meeting Room 12
3:15 pm – 5:30 pm 2019 In Vitro Biology Meeting Opening Ceremony

3:15 Welcome and Opening Remarks:
3:25 Robert H. Lawrence, Jr. Keynote Symposium
4:15 Questions and Answers
4:30 2019 Awards Ceremony
4:35 Distinguished, Fellow, and Young Scientist Award
Awards to be presented at Section Meetings
4:40 Distinguished Service Award Presentations
5:00 Lifetime Achievement Award Presentations
5:30 Group photo with Dr. Medford and Student attendees
Grand Salon F
Evening 5:30 pm – 9:30 pm
5:30 pm – 6:30 pm Opening Ceremony Reception Grand Salons A-E
6:30 pm – 7:30 pm Poster Session
Odd Poster Authors will be present
Grand Salons A-E
7:30 pm – 9:30 pm Student Workshop: Hands-on RNA-Seq Workshop Using the CyVerse Computational Infrastructure Meeting Rooms 8&9

Sunday, June 9

7:00 am – 5:30 pm Registration Grand Salons A-D Foyer


FRONTIERS OF IN VITRO AND SYNTHETIC BIOLOGY

Conveners:     Fredy Altpeter, University of Florida, and Michael K. Dame, University of Michigan Medical School

 

 8:00 am – 10:00 am  Plenary Symposium  Grand Salon F

Synthetic biology applies engineering principles to biology by targeting the design and construction of new biological parts, devices, and systems, and the re-design of existing, natural biological systems for broad applications including biofuels, agriculture, bio-materials, biopharmaceuticals, sustainability and more. Session topics will focus on development of tools to enable rational design of novel traits. The session will also present recently pioneered human organoid systems, derived from three-dimensional cell cultures or biopsy tissue samples. These unique human “organs in a dish” incorporate some of the key features of the represented organs and are powerful tools to study and understand molecular mechanisms controlling developmental processes, stem/progenitor cell regulation, cell fate decision-making, disease, and repair.

8:00

Introduction (F. Altpeter and M. K. Dame)

8:05 PS-1 Beyond Gene Editing: Current Status and Future Applications of Synthetic Biology in Plants
C. Neal Stewart, Jr.,  University of Tennessee
8:40 PS-2 Developing Plant Synthetic Biology Tools for Complex Metabolic Engineering
Patrick Shih, The Joint Bioenergy Institute
9:15 PS-3 Profiling the Diversity and Development of Human Organs and Organoids at Single Cell Resolution
Jason Spence, University of Michigan
9:50 Discussion


10:00 am – 10:30 am Coffee Break Grand Salons A-E

BIOLOGICAL SENSORS: ORGANOIDS TO ORGANISMS FOR ANSWERING MEDICINAL, AGRICULTURAL AND ENVIRONMENTAL QUESTIONS

Conveners:     Allan R. Wenck, BASF, and Michael K. Dame, University of Michigan Medical School

10:30 am – 12:30 pm Joint Symposium Meeting Room 12

3D organoid cultures established from patient-derived tissues serve as an highly accurate model to assess efficacy and safety of various agents across multiple organ systems. In a breast stem cell-derived organoid model, as well as in 2D primary monolayers, the impact of the environmental risk factor cadmium is characterized across racially diverse donors. Secondly, a study employing patient-derived colon organoids, evaluates a red marine algae product, currently in clinical trial, for colon cancer chemopreventative properties. The enhancement of the intestinal barrier properties are assessed with a 2D transwell monolayer system. Finally, work will be presented here demonstrating the novel use of whole plant organisms to screen environmental toxins. These sophisticated multicellular models have the potential to accurately evaluate responses across many applications and questions.

10:30 Introduction (A. R. Wenck and M. K. Dame)
10:35 J-1 2D and 3D Primary Human Breast Tissue Culture Models to Understand Environmental Impacts on Stem Cells in Cancer
Justin Colacino, University of Michigan School of Public Health
11:10 J-2 Differentiation in the Colonic Mucosa with a Mineral Supplement Derived from the Marine Algae Lithothamnion sp.: Clinical Trial Outcomes Compared to Response in Human Colonoid Culture
James Varani, University of Michigan
11:45 J-3 TBD
C. Neal Stewart, Jr.,  University of Tennessee
12:20 Discussion


ENGINEERING STRESS TOLERANCE IN CROP PLANTS

Conveners:     Sadanand Dhekney, University of Maryland Eastern Shore, and Prakash Kumar, National University of Singapore

10:30 am – 12:30 pm Plant Symposium Grand Salons G-J

Plant growth and productivity are under constant threat by the environmental challenges. Some of the crucial factors that have a major influence on plants include abiotic stresses such as salinity, drought, and cold. Salinity stress alone affects plant growth at various developmental stages leading to a reduction in crop yield. With the rapidly growing population, improving crop tolerance to abiotic stress will be a key solution to increase agricultural production. Technological advances in recent years have resulted in the identification of several biomarkers that control plant growth and development and play a crucial role during abiotic stresses. Enhanced expression of some of the genes that regulate synthesis of different osmolytes and sugars, e.g., trehalose has been shown to confer salinity tolerance in crop plants. The involvement of ABA signalling in abiotic stress tolerance is well known. However, the various signalling players involved are only partly known. Talks in this session will focus on identifying genes and molecular mechanism underlying stress response, and how selected candidate genes may help in generating crop plants with enhanced abiotic stress tolerance in the coming decades.

10:30 Introduction (S. Dhekney and P. Kumar)
10:35 P-1 Use of OsTPS8 for Molecular Genetic Enhancement of Rice Yield and Resilience Under Salt Stress
Prakash Kumar, National University of Singapore
11:10 P-2 H+-PPases Are Key Versatile Enzymes Involved in Carbohydrates Translocation and Metabolism in Plants
Roberto Gaxiola, Arizona State University
11:45 P-3 Translating Transcriptome Resources of a Grass Extremeophile to Engineer Salt and Drought Tolerant Rice
Niranjan Baisakh, Louisiana State University Agricultural Center
12:20 Discussion


MICROPROPAGATION BEST PRACTICES

Conveners:     Angela Labrum, Carlton Plants Nursery, and Joyce Van Eck, The Boyce Thompson Institute

10:30 am – 12:30 pm Plant Symposium Grand Salon F

Micropropagation has many applications including, but not limited to, conservation efforts of threatened and endangered species and generation of large numbers of plants for nursery production.  Factors such as the health of the starting material, medium components, and environmental factors (lighting and temperature) can influence in vitro plant growth.  The speakers in this session will share some of the most recent advances related to the effects of these factors on micropropagation and share information related to case studies where these advances were put into practice.   The topics will include stock plant maintenance, insect control, approaches for contaminant elimination and acclimatization.

10:30 Introduction (A. Labrum and J. Van Eck)
10:35 P-4 Stock Plant Maintenance and Initiation
Kara Gregory, Spring Meadow Nursery
11:10 P-5 In-Vitro Insect Control
Calvin Anderson, Better Blooming Orchids
11:45 P-6 Quality Plants for Acclimatization
Ray Gillis, Oglesby Plants International
12:20 Discussion


MICROPROPAGATION BEST PRACTICES PANEL DISCUSSION

Moderators: Angela Labrum, Carlton Plants Nursery, and Joyce Van Eck, The Boyce Thompson Institute

12:30 pm – 1:30 pm Plant Panel Discussion Meeting Rooms 8&9

We hope you will join us for an open format question and answer session immediately following the Best Practices Session. There will be three micropropagation professionals serving as panel members to facilitate the discussion. Our desire is that this is a time of problem solving, idea sharing and networking.

Panelists:
Steve McCulloch, Mountain Shadow Nursery
David Lawson, Agri Starts
Barbara M. Reed, Retired, USDA

IN VITRO BIOTECHNOLOGY

Moderator:      Jessica L. Rupp, Kansas State University

1:30 pm – 2:45 pm Plant Contributed Paper Session Grand Salon F
1:30 P-1000 In Vitro and In Silico Screening of Potential Cancer Inhibitors from Cullen corylifolium (L.) Medik., Their Isolation and Elicitation Through Cotyledon-Callus Cultures
Veena Agrawal, University of Delhi, and Tikkam Singh
1:45 P-1001 Assessment of Thiaminase and Arginase on Mesocarp and Seeds of Cherry Tomato
Ajiboye Abiodun Akeem, Federal University Oye-Ekiti, O. O. Oketayo, O. A. David, J. O. Agbolade, T. O. Ajewole, M. A. Adekoya, R. J. Komolafe, and B. Ofoyebi
2:00 P-1002 Beneficial Interactions of P. putida with Different Dicot Plants in In Vitro Conditions
Özlem Akkaya, Gebze Technical University, and Ebru Arslan
2:15 P-1003 Development of Insect Resistant Potato Transplastomic Lines
Allah Bakhsh, Nigde Omer Halisdemir University, Faiz Ahmad Joyia, Md. Jakir Hossain, Emre Aksoy, Neslihan Zahide Öztürk Gökçe, Ghulam Mustafa, and Muhammad Sarwar Khan
2:30 P-1004 Cloning and Characterization of Dehydrin Genes from Atriplex Species
Monther T. Sadder, University of Jordan, Anas Musallam, and Saeid Abu-Romman

METABOLIC ENGINEERING FOR VALUE ADDED PLANT PRODUCTS AND BIOFUELS

Convener:       J. Pon Samuel, CORTEVA AgriscienceTM, Agriculture Division of DowDuPontTM, and Ming Cheng, BASF Plant Sciences

1:30 pm – 3:00 pm Plant Symposium Grand Salons G-J

Metabolic engineering is the targeted improvement of the cellular metabolic pathway using recombinant DNA technology with the goal of high-yield production of value added plant products including fuels. Using synthetic biology approaches facilitates the modular assembly of multigenic constructs and allows programming of plant cells or microbes with more precise control and more dynamic gene expression for generating plant products that are scalable and thus commercially viable. In the recent past, metabolic engineering has scaled up production of fragrances, food flavors, medicinal components or fuels. This session emphasizes targeted pathway engineering for high-yield production of plant-based metabolites and fuels with options for next generation products in conventional and nonconventional platforms.

1:30 Introduction (J. P. Samuel and M. Cheng)
1:35 P-7 Engineering Microbes for Agricultural Products
Babu Raman, CORTEVA AgriscienceTM, Agriculture Division of DowDuPontTM
2:00 P-8 Converting Sugarcane into Oilcane by Metabolic Engineering
Fredy Altpeter, University of Florida
2:25 P-9 Systematic Genetic Engineering Approaches for Improvement of Full Scale Fermentation Economics
Patrick Westfall, Zymergen
2:50 Discussion


STEM CELL DIFFERENTIATION IN HUMAN MODELS

Conveners:    Tetsuji Okamoto, Hiroshima University, and J. Denry Sato, Manzanar Project Foundation

1:30 pm – 3:00 pm Animal Symposium Meeting Room 12

The demonstrations that human pluripotent stem cell lines could be isolated from embryos (ES) or induced from differentiated cells (iPS) have held out the prospect and promise of cellular therapies for degenerative or genetic diseases. In addition, the ability to generate iPS cell lines from patients with genetic diseases allows those diseases to be studied with new precision. This session will address a method to generate disease-specific iPS cell lines without genetically transforming them and developing disease models based on differentiation of iPS cells. Pluripotent stem cells may hold promise for future cellular therapies, but multipotent adult stem cells may be more likely to give rise to therapies in the near term. The last talk will focus on the application of oral and craniofacial stem cells in regeneration.

1:30 Introduction (T. Okamoto and J. D. Sato)
1:35 A-1 Establishment and Characterization of Disease-specific Human iPSCs in Serum-, Integration- and Feeder-free Cultures
Atsuko Hamada, Hiroshima University
2:00 A-2 Disease Modeling Using Disease-specific iPS Cell Collection in RIKEN Cell Bank
Yohei Hayashi, RIKEN BioResource Research Center
2:25 A-3 Application of Neural Crest Stem-like Cells Induced from Human Gingiva-derived MSCs in Peripheral Nerve Regeneration
Qunzhou Zhang, University of Pennsylvania School of Dental Medicine
2:50 Discussion


 

2019 IN VITRO BIOLOGY MEETING OPENING CEREMONY

Program Chair: Fredy Altpeter, University of Florida

3:15 pm – 5:30 pm Opening Ceremony Grand Salon F

Conveners: Fredy Altpeter, University of Florida, and John W. Harbell, JHarbell Consulting

3:15 Welcome and Opening Remarks:
John W. Harbell, President, Society for In Vitro Biology
3:25 KS-1 ROBERT H. LAWRENCE, JR. KEYNOTE SYMPOSIUM

Introduction (F. Altpeter)

Synthetic Biology for Engineering Plant Genetic Circuits: from Predictable Electronic-like Functions to Innovative Desalination
June Medford, Professor of Biology at Colorado State University

4:15 Questions and Answers
4:30 2019 SOCIETY FOR IN VITRO BIOLOGY AWARDS CEREMONY
John W. Harbell, President, Society for In Vitro Biology
4:35 2019 Distinguished, Fellow, and Young Scientist Award Recipients (Awards to be presented at Section Meetings)

Distinguished Scientist: Harold N. Trick, Kansas State University
Fellow: Jeffrey W. Adelberg, Clemson University
C. Neal Stewart, Jr., University of Tennessee
Young Scientist: Raj Deepika Chauhan, Pairwise
4:40 2019 Distinguished Service Award Presentations
(Awards to be presented by John W. Harbell):
Barbara B. Doonan, New York Medical College
Maria M. Jenderek, USDA-ARS
Albert P. Kausch, University of Rhode Island
Pamela J. Weathers, Worchester Polytechnic Institute
5:00 2019 Lifetime Achievement Award Presentations:
(Introduction by J. Denry Sato, Acknowledgement by Dr. Okamoto to follow)
Tetsuji Okamoto, Hiroshima University
 5:15 (Introduction by Valerie C. Pence; Acknowledgement by Dr. Reed to follow)
Barbara M. Reed, Retired, USDA
 5:30 Adjourn
Group photo with Dr. Medford and Student attendees
5:30 pm – 6:30 pm 2019 IN VITRO BIOLOGY MEETING
OPENING CEREMONY RECEPTION
Grand Salons A-E

5:30 pm – 7:30 pm Exhibits and Posters Grand Salons A-E

Sunday, June 9
Odd Poster Authors will be present
6:30 pm – 7:30 pm
 


STUDENT WORKSHOP: HANDS-ON RNA-SEQ WORKSHOP USING THE CYVERSE COMPUTATIONAL INFRASTRUCTURE

Moderators: Adrianne Brown, Tuskegee University, and Sepideh Mohammadhosseinpour, Arkansas State University

7:30 pm – 9:30 pm Education Workshop Meeting Rooms 8&9

RNA-Seq is a powerful and easily assessable method to examine and compare gene expression on a broad scale. This workshop will focus on introducing researchers to the principles behind RNA-Seq analysis and will provide a hands-on introduction to software and analysis pipelines for RNA-Seq. The workshop is aimed at teaching the computational process that takes the raw data all the way through the high level analysis. Theory and hands-on application will be centered around two RNA-Seq analysis suites: (1) Cufflinks and Stingtie are expression analysis modules that assemble raw reads into transcripts and then evaluate expression, and (2) DeSeq2 which is an R package that works directly on count data.  There is no pre-requisite programming experience necessary. Raw and analyzed data will be hosted on CyVerse – an NSF funded life sciences cyber-infrastructure platform – that allows analysis to be performed in a web-based environment. Data analysis and demonstrations will be performed on the CyVerse Discovery Environment (DE). For the best experience, students are requested to establish a free CyVerse account (www.cyverse.org) prior to the workshop and should have access to a networked laptop.

7:30 Introduction (A. Brown and S. Mohammadhosseinpour)
7:35 E-1 Hands-on RNA-Seq Workshop Using the CyVerse Computational Infrastructure
William Bradley Barbazuk, University of Florida

 

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