Monday, June 10

Daily Program-at-a-Glance

Time Event Location
7:00 am – 6:00 pm Registration Grand Salons A-D Foyer
10:00 am – 3:30 pm Exhibits and Posters Grand Salons A-E
Morning 7:00 am – 12:30 pm
7:00 am – 8:00 am Plant Biotechnology Program Committee Meeting Meeting Rooms 8&9
Publications Committee Meeting Meeting Room 10
8:00 am – 10:00 am Delivery of Genome Editing Reagents Grand Salon F
10:00 am – 10:30 am Coffee Break Grand Salons A-E
Awards Committee Meeting Meeting Room 11
10:30 am – 12:10 pm Plant Biotechnology Post Doctoral Oral Presentation Competition Meeting Rooms 8&9
 10:30 am – 12:30 pm 3D Toxicology: Emerging Technologies Directed Towards the Prediction of Human Responses Meeting Room 12
Plant Biotechnology Student Oral Presentation Competition Grand Salons G-J
Genome Editing for Crop Improvement Grand Salon F
Afternoon 12:30 pm – 6:00 pm
12:30 pm – 1:30 pm Student Networking Luncheon: Employer Engagement Meeting Rooms 8&9
Long Range Planning Committee Meeting Meeting Room 10
Refreshment Break Grand Salons A-E
1:30 pm – 2:30 pm In Vitro Animal Cell Sciences Student and Post Doctoral Oral Presentation Competition Meeting Room 12
Interactive Poster Sessions

In Vitro
Genetic Transformation

Grand Salons A-E
2:30 pm – 3:00 pm Animal Poster Session
Animal Even Poster Authors will be present
Grand Salons A-E
 2:30 pm – 3:30 pm Education and Plant Poster Session
Even Poster Authors will be present
Grand Salons A-E
 3:30 pm – 5:00 pm Importance of Signaling Molecules in Establishing Cell Cultures Meeting Room 12
Protoplast Technology for Genome Editing Grand Salon F
Controlled Environments for Plant Tissue Culture Grand Salons G-J
Non-competitive Student Oral Presentations Meeting Rooms 8&9
5:00 pm – 6:00 pm In Vitro Animal Cell Sciences Contributed Papers Meeting Room 12
Late Breaking Topics/Young Investigator Presentations in Plant Biotech Grand Salon F
Micropropagation Grand Salons G-J
Evening 6:00 pm – 10:00 pm
6:00 pm – 7:30 pm In Vitro Animal Cell Sciences Section Meeting Meeting Room 12
Plant Biotechnology Section Meeting Meeting Rooms 8&9
7:30 pm – 10:00 pm Joint Sections Social Il Terrazzo
8:30 pm – 9:30 pm Panel Discussion: Future Directions in Development and Applications of Cell Lines Meeting Room 12

Monday, June 10

7:00 am – 6:00 pm Registration Grand Salons A-D Foyer


DELIVERY OF GENOME EDITING REAGENTS

Conveners: Ajith Anand, CORTEVA AgriscienceTM, Agriculture Division of DowDuPontTM, and Joshua Gasiorowski, Midwestern University

8:00 am – 10:00 am Plenary Symposium Grand Salon F

Genome editing technology is powerful tool for modern biotechnology with the potential to modify microbial, plant and animal genomes. The targeted modification of genomic sequences has been accomplished using programmable nucleases, including Meganucleases, zin finer nuclease (ZFNs), transcription activator-like nucleases (TALENs), and the clustered regularly-interspaced short palindromic repeat-associated nuclease Cas9 (CRISPR-Cas9). There is a good understanding of the basic mechanisms of the CRISPR/Cas9 system and its predecessors (Meganucleases, ZFNs and TALENs). Unfortunately, one of the major limitations in genome editing is the ability to “deliver” the different cargo types and editing reagents to a target cell. This session will deliberate on the various vehicles and techniques reported for biomolecule and nucleic acid delivery and discuss their relative merits.  Our speakers will discuss very recent advances using electroporation, diffusion-based, and nanoparticle-based delivery methods currently being explored to effectively introduce genes and genome editing reagents into plant and animal cells for agricultural and medical applications.

8:00

Introduction (A. Anand and J. Gasiorowski)

8:05 PS-4 Enhanced Gene Delivery Utilizing Non-viral Approaches
Richard Heller, Old Dominion University
8:40 PS-5 High Aspect Ratio Nanomaterials Enable Delivery of Functional Genetic Material Without DNA Integration in Mature Plants
Markita del Carpio Landry, University of California – Berkeley
9:15 PS-6 Current and Future Gene Editing Delivery Methods for Plant Genome Modification
Doane Chilcoat, Applied Science and Technology, Corteva AgriscienceTM, Agriculture Division of DowDuPontTM
9:50 Discussion


10:00 am – 10:30 pm Coffee Break Grand Salons A-E

3D TOXICOLOGY: EMERGING TECHNOLOGIES DIRECTED TOWARDS THE PREDICTION OF HUMAN RESPONSES

 

Conveners: John W. Harbell, JHarbell Consulting LLC, and Seyoum Ayehuine, MatTek Corporation

10:30 am – 12:30 pm Animal Symposium Meeting Room 12

Toxicology is moving from an observational science based on macro-responses of surrogate animal species to one based on hypothesis testing to predict effects in the actual species of interest (often humans). This change is by no means complete but is being driven by an understanding that much of the toxic response is based perturbation or activation of critical pathways (Toxicology in the 21st Century) that can be quantitatively different in the species of interest. Understanding these pathways provides physiologically-based guidance for identifying upstream endpoint measures predictive of downstream effects. The proper selection of endpoint measures has become a key element in the development of in vitro toxicological methods. It has also become clear that the exposure kinetics of the target tissue (test system) and the required pathways can be dependent on the 3-dimensional (3D) milieu of that test system. The 3D architecture has a polarized tissue structure, allows more normal cell-cell communication, can include multiple cell types, is characteristic of the tissue in vivo, and provides progressive exposure into the tissue not possible with a single layer of cells. This symposium will focus on next generation of in vitro test methods with particular focus on the emerging technologies in 3D tissue systems and the application of these systems to regulatory toxicology. In the past, developers of in vitro systems (2D or 3D) brought their assays to the regulatory agencies without extensive input from those agencies. Now the agencies are poised to be active participants in the development process and to provide guidance as to specific agency needs.

10:30 Introduction (J. W. Harbell and S. Ayehuine)
10:35 A-4 3D Tissue Models a Critical Component of Regulatory Toxicology in the 21st Century
Warren Casey, National Institute for Environmental Sciences
11:10 A-5 Cutaneous Models for Evaluating Pollution Induced Skin OxInflammation
Guiseppe Valacchi, North Carolina State University
11:45 A-6 Three Dimensional Test Systems for Predicting the Degree of Corneal Injury from Topical Exposure to Chemicals
John W. Harbell, JHarbell Consulting LLC
12:20 Discussion


PLANT BIOTECHNOLOGY POST DOCTORAL ORAL PRESENTATION COMPETITION

Moderator: Geny Anthony, CORTEVA AgriscienceTM, Agriculture Division of DowDuPontTM

10:30 am – 12:10 pm Plant Contributed Paper Competition Meeting Rooms 8&9

Panel of Judges reviewing the presentations: Prakash Kumar, University of Singapore, Lori Marcum, CORTEVA AgriscienceTM, Agriculture Division of DowDuPontTM, and Nagesh Sardesai, CORTEVA AgriscienceTM, Agriculture Division of DowDuPontTM

To support the Society’s vision to encourage education and scientific informational exchange and recognize outstanding post docs, the Plant Biotechnology Section is pleased to announce the 2019 Post-Doctoral Oral Presentation Competition. Competition finalists were selected based on the quality of the abstracts. A panel of judges will evaluate the presentations at the meeting. Criteria for the evaluation include experimental design, data analysis, proper interpretation of the results, originality of the study, technical difficulty, appearance and ability of the student candidate to present it. Winners will be presented with a certificate and a cash award at the meeting.

10:30 P-1005 Application of Protoplast Technology for Genome Editing in Physalis Species
Nathan T. Reem, Boyce Thompson Institute, Linnell B. Randall, and Joyce Van Eck
10:50 P-1006 Transcriptomic Response of the Novel Plant Engineering Bacterium Ensifer adhaerens OV14 During Colonization of A. thaliana roots
Evelyn Zuniga-Soto, Teagasc and University College Dublin, David Fitzpatrick, Fiona Doohan, and Ewen Mullins
11:10 P-1007 Improving Homology Directed Repair Mediated Genome Editing in Plants
Raviraj Banakar, University of Minnesota, Xiaojun Kang, and Feng Zhang
11:30 P-1008 This presentation is not participating as part of the Post Doctoral competition.
Towards Engineering Multiple Stress Tolerance in Vigna mungo (Black gram) Utilizing the Aldose Reductase Gene from a Resurrection Plant
D. Kumar, Central University of Jammu, P. Singh, and N. B. Sarin
11:50 P-1009 GAANTRY: A Precise and Robust Agrobacterium-based Gene Stacking System for Crop Improvement
Leyla Hathwaik, USDA-ARS, James Thomson, and Roger Thilmony

PLANT BIOTECHNOLOGY STUDENT ORAL PRESENTATION COMPETITION

Moderator: Veena Veena, Donald Danforth Plant Science Center

10:30 am – 12:30 pm Plant Contributed Paper Competition Grand Salons G-J

Panel of Plant Biotechnology Experts Evaluating the Contestants: Ajith Anand, CORTEVA AgriscienceTM, Agriculture Division of DowDuPontTM, Carlos Hernandez-Garcia, CTC Genomics, and Randall P. Niedz, USDA-US Horticultural Research Laboratory

To support the Society’s vision to encourage education and scientific informational exchange and recognize outstanding students, the Plant Biotechnology Section is pleased to announce the 2019 Student Oral Presentation Competition. Student competition finalists were selected based on the quality of the abstracts. A panel of judges will evaluate the presentations at the meeting. Criteria for the evaluation include experimental design, data analysis, proper interpretation of the results, originality of the study, technical difficulty, appearance and ability of the student candidate to present it. Winners will be presented with a certificate and a cash award at the meeting.

10:30 P-1010 Knockout of Potato Invertase Inhibitor Gene by CRISPR/CAS9 Based Approach
S. D. Dangol, Nigde Omer Halisdemir University, A. Barakate, M. E. Caliskan, and A. Bakhsh
10:50 P-1011 Author is unable to attend
11:10 P-1012 Development of Variegated Lettuce Using CRISPR/Cas9 Technology
Chi L Nguyen, University of Florida, Juncheng Li, Shoumian Li, Baiquan Mou, and Heqiang Huo
11:30 P-1013 Development and Validation of a Novel Soybean (Glycine max (L.) Merr.) Cell Suspension Culture for High-throughput Promoter Screening
Mst Shamira Sultana, University of Tennessee, Taylor P. Frazier-Douglas, Reginald J. Millwood, Scott C. Lenaghan, and C. Neal Stewart, Jr.
11:50 P-1014 Multiplex Genome Editing in the Illinois Long Term Selection Experiment
Stephen J. Jinga, University of Illinois Urbana-Champaign, Brian Rhodes, Eddie Ross, Anna Parkinson, and Stephen Moose
12:10 P-1015 In Vitro Propagation of Asclepias spp. for Monarch Butterfly Habitat Restoration
Angelo Alvarez, Los Angeles Pierce College, Savannah St. Clair, John Creedon, Elnura Maine, and Daila Melendez

GENOME EDITING FOR CROP IMPROVEMENT

Conveners: Kan Wang, Iowa State University, and Harold N. Trick, Kansas State University

10:30 am – 12:30 pm Plant Symposium Grand Salon F

Innovations in gene editing and its application in crop engineering have tremendous potential in terms of increasing crop production, enhancing crop protection, and advancing breeding programs. In the symposium we will hear from both academic and industrial scientists who are using gene editing in their research programs. Their progress and lessons learnt from the process can provide the community with new insight for future success.

10:30 Introduction (K. Wang and H. N. Trick)
10:35 P-10 Applications of Gene Editing to Improve Yield Component Traits in Wheat
Wei Wang, Kansas State University
11:00 P-11 Genome Editing: A Powerful Tool for Ideotype Breeding in Polyploid Grasses
Shuizhang Fei, Iowa State University
11:25 P-12 Improvement of Goldenberry and Groundcherry by CRISPR: Ripening the Potential of Underutilized Fruit Crops
Joyce Van Eck, The Boyce Thompson Institute
11:50 P-13 Improving Targeted Genome Optimization in Plants
Allan R. Wenck, BASF
12:15 Discussion


STUDENT NETWORKING LUNCHEON: EMPLOYER ENGAGEMENT

Conveners: Adrianne Brown, Tuskegee University, and Sepideh Mohammadhosseinpour, Arkansas State University

12:30 pm – 1:30 pm Education Workshop Meeting Rooms 8&9

Have you been looking for a dream job and/or research position as a graduate student? Have you been searching for networking opportunities or even thought about your future and who can guide you through it? If that’s the case, this is your golden opportunity! Come to the student networking luncheon to find all the answers you have been waiting for. This luncheon will provide students with a chance to interact with professors and experts from various fields, including academia and industry. If a career in academia is what you are seeking, then doctors will be available to assist you and provide you with information about how to designate your resume, research opportunities and grant writing. If students are interested in an industry position, then selected employers will provide you with the proper feedback needed to select major requirements, develop skills, interview questions, etc. During the close, professionals will also share their academic experiences and discuss stressful experiences and how they have coped while pursuing their graduate degrees. Generally, this workshop could simply introduce you to your new research mentor or your boss, collaborator and external internship possibility.

 12:30 Introduction (A. Brown and Sepideh Mohammadhosseinpour)
 12:35 Panel Discussion


IN VITRO ANIMAL CELL SCIENCES STUDENT AND POST DOCTORAL ORAL PRESENTATION COMPETITION

Moderators: Addy Alt-Holland, Tufts University, and Kolla Kristjansdottir, Midwestern University

1:30 pm – 2:30 pm Animal Contributed Paper Competition Meeting Room 12

Panel of In Vitro Animal Cell Sciences Experts Evaluating the Contestants: Addy Alt-Holland, Tufts University, Kolla Kristjansdottir, Midwestern University, John W. Harbell, JHarbell Consulting, LLC, Barbara Doonan, New York Medical College, Michael J. Fay, Midwestern University, Barbara B. Doonan, New York Medical College, Brad L. Upham, Michigan State University, Michael Dame, University of Michigan Medical School, Mae Ciancio, Midwestern University, and Joshua Gasiorowski, Midwestern University

The In Vitro Animal Cell Sciences Section of the Society for In Vitro Biology is pleased to announce the 2019 Student and Post-Doctoral Oral Presentation Competition. This competition serves as a platform to recognize and reward the research work and achievements of outstanding students and talented post-docs. It aims to encourage the exchange of scientific information during the conference and provides an invaluable opportunity for students and post-docs to enhance their public speaking and presentation skills. The competition will include three finalists selected based on the quality of the abstracts and the merit of the scientific findings. During the Oral Presentation Competition session, a panel of expert judges will grade the presentations. Criteria that would be evaluated include the experimental design, data analysis, proper interpretation of the results, originality of the study, technical difficulty, professionalism and ability of the finalist to explain the research work and answer questions, as well as adherence to the allocated time for the presentation. The three finalists will be presented with a certificate and a cash award during the conference.

1:30 A-1000 Author is unable to attend
 1:50 A-1001 Dietary Supplementation with Anthocyanin-rich Berries Promotes Healthy Muscle Development Gene Expression Profiles in Diet-induced Obese Mice
Jia Xiong, North Carolina State University, John Overall, Slavko Komarnytsky, and Debora Esposito
 2:10 A-1002 Characterization of Polymeric Electrospun Fibers for Tissue Engineering and Biologic Delivery
Alyssa G. Togliatti, Midwestern University, and Joshua Z. Gasiorowski

IN VITRO

Moderator:     Randall P. Niedz, USDA-US Horticultural Research Laboratory

1:30 pm – 2:30 pm Plant Interactive Poster Session Grand Salons A-E
P-2000 Effect of Light Quality and Intensity on Plant Growth and Essential Oil of Lippia rotundifolia
José Eduardo Pinto, Lavras University, Bety S. de Hsie, Ana Izabela S. Bueno, Alexandre A. de Carvalho, and Suzan Kelly V. Bertolucci
P-2001 Unraveling the Biosynthesis of Prenylated Stilbenoids in Peanut and Their Anti-inflammatory Activities In Vitro
Rokib Hasan, Arkansas State University and Arkansas Bioscience Institute, Maria Elena Gonzalez Romero, and Fabricio Medina-Bolivar  
P-2002 Grafted Aptenia cordifolia (L. f.) Schwant Leaves as Perfusable Tissue Engineered Scaffolds
Yueqing Wang, Worcester Polytechnic Institute, Tanja Dominko, Glenn R. Gaudette, and Pamela J. Weathers
P-2003 Hydroxyproline-O-glycan Engineering in Tobacco Transient Protein Expression: Fish IL-22 and eGFP as Model Systems
Cristofer Calvo, Arkansas State Bioscience Institute, Jianfeng Xu, and Maureen C. Dolan
P-2004 Investigation of Physiological Mechanisms in Drought Tolerant Digitaria exilis
Oyinade Aderoju David, Federal University Oye-Ekiti,and Oluwole Osonubi
P-2005 Development of an Efficient Strategy to Produce Spray Dried Lycopene-rich Watermelon-protein Particles: In Vitro Evaluation of Phytochemicals and Skin Fibroblast Migration
Roberta T. Hoskin, Federal University of Rio Grande do Norte (UFRN), Jia Xiong, Penelope Perkins-Veazie, and Debora A. Esposito

GENETIC TRANSFORMATION

Moderator:     Sadanand Dhekney, University of Maryland, Eastern Shore

1:30 pm – 2:30 pm Plant Interactive Poster Session Grand Salons A-E


P-2013 Replicon Vectors for Whole-plant Transformation in Monocots
James Chamness, University of Minnesota, Nathaniel Graham, Hiroaki Saika, Matthew Nelson, and Daniel Voytas
P-2014 Establishment of Agrobacterium-mediated Genetic Transformation of Miscanthus sinensis
Prabin Dhungana, Mississippi State University,and Nancy A. Reichert
P-2015 Understanding Agrobacterium and Soybean Interactions to Overcome Poor Transformation
Timothy M. Chappell, University of Georgia, Brian Kvitko, Zenglu Li, and Wayne Parrott
P-2017 Developing a CRISPR Protocol for Blueberry Vaccinium corymbosum L.
Francesco Cappai, University of Florida, Ryan Cullen, Alexandria Garcia, Adina Grossman, Heqiang Huo, and Patricio Munoz
P-2018 Plant Genetic Transformation Protocols: An Essential Platform and Bottleneck for Genome Editing
Murray Boase, The New Zealand Institute for Plant and Food Research Limited, Huaibi Zhang, Lei Wang, Ian King, Steve Arathoon, Nick Albert, Sarina Manandhar, David Lewis, Kathy Schwinn, Kevin Davies, and Simon Deroles

Monday, June 10
Even Poster Authors will be present
2:30 pm – 3:30 pm

 

IMPORTANCE OF SIGNALING MOLECULES IN ESTABLISHING CELL CULTURES

Conveners:   Cynthia L. Goodman, USDA, ARS, BCIRL, and Guy Smagghe, Ghent University

SPONSORED BY BASF

3:30 pm – 5:00 pm Animal Symposium Meeting Room 12

Cell cultures serve as valuable tools in agricultural and medical research for both basic and applied studies.  They are also heavily used by the industrial sector in high throughput screening assays, mechanism of action studies, and other aspects of product development.  The focus of this session will be on the development of cell cultures that express phenotypes predictive of in vivo responses.  The first two speakers will discuss the initiation of continuously replicating cell lines, as well as primary cultures, for agricultural applications.  The last two speakers will outline signaling transduction mechanisms and their relationship with physiological processes, such as immunity, and cell fate/differentiation. There will also be a discussion on the use of genetic modifications to encourage the development of tissue-specific cell lines.

3:30 A-8 Specific Distribution of Glycan Motifs at the Surface of Midgut Cells in the Cotton Leafworm
Guy Smagghe, Ghent University
3:55 A-9 Intracellular Cross-talk in Insect Immune Signaling
David Stanley, 
USDA, ARS, BCIRL
4:20  A-10 Genetic Approaches to Rapid Cell Line Generation in Drosophila
Amanda Simcox, 
The Ohio State University
4:45 Discussion


PROTOPLAST TECHNOLOGY FOR GENOME EDITING

Conveners:      Ian S. Curtis, Plastomics, and Carlos M. Hernandez-Garcia, CTC Genomics

3:30 pm – 5:00 pm Plant Symposium Grand Salon F

Protoplasts are osmotically fragile cells which have had their cell wall removed usually by digestion with enzymes. Isolated protoplasts are unique to a wide range of experimental procedures such as protoplast fusion enabling nuclear and cytoplasmic genomes to be combined and DNA transfer for transient and stable transformation in the nucleus and also organelle transformation to produce transplasmotic plants. Several parameters, particularly source of tissue, culture medium, environmental factors, influence the ability of a protoplast to develop into a fertile plant. However, despite over 50 years of research, many species remain recalcitrant in culture. Recently, there has been a resurgence in protoplast systems for rapid screening of gene silencing events through dsRNA, miRNA and siRNA and genome-editing using clustered regularly interspaced short palindromic repeats (CRISPR) and transcription activator-like effector nucleases (TALENS) using high-throughput automated platforms. In this session, we are going to showcase the recent advances in how protoplasts are being used in genomic research to accelerate and design crop improvement.

3:30 Introduction (I S. Curtis and C. M. Hernandez-Garcia)
3:35 P-14 Improving HDR-mediated Genome Editing Through Plant Protoplast Engineering
Feng Zhang, University of Minnesota
4:00 P-15 Plant Protoplast Automation: Production, Transfection and Screening
C. Neal Stewart, Jr., University of Tennessee
4:25 P-16 Application of Protoplast Technology to Plant Improvement in the 21st Century
Jude Grosser, University of Florida
4:50 Discussion


CONTROLLED ENVIRONMENTS FOR PLANT TISSUE CULTURE

Conveners: Steve McCulloch, Mountain Shadow Nursery LLC, and Jeffrey Beringer, Inari

3:30 pm – 5:00 pm Plant Symposium Grand Salons G-J

Environmental factors present in the tissue culture space that may influence the successful growth of in vitro plant cultures are often discounted or not seriously considered by researchers.  Light quality, variability in temperature control, humidity, and gas exchange are all influenced by the choice of engineering controls.  Managing all these factors to optimize culture growth can be a complex task.  Researchers must make a multitude of design choices to achieve their goals.  This session will examine various aspects of manipulating the environment that in vitro plants are grown in and how researchers can use this to their advantage.

3:30 Introduction (S. McCulloch and J. Beringer)
3:35  P-17 Engineering and Sciences in Controlled-Environment Agriculture: Lessons Learned
Yang Yang, Purdue University
4:00  P-18 LED Lighting for Indoor Plant Propagation
Eric Runkle, Michigan State University
4:25 Discussion with Panel of Industry Experts:
Ray Gillis, Oglesby Plants International
Jonathan Jasinski, Microplant Nurseries
Lisa Kovski-Alamillo, Carlton Plants
David Lawson, AgriStarts
Gayle Suttle, Microplant Nurseries

NON-COMPETITIVE STUDENT ORAL PRESENTATIONS

Moderators: Adrianne Brown, Tuskegee University, and Sepideh Mohammadhosseinpour, Arkansas State University

3:30 pm – 5:00 pm Education Symposium Meeting Rooms 8-9

The Society for In Vitro Biology (SIVB) takes pride in showcasing some of the most innovative research topics in both plant and animal sciences. Our mission for the non-competitive oral presentations are to provide a platform for student researchers to achieve recognition and share their research work with their peers as well as academic and industry professionals, who can take their projects to the next level without the pressure of competing. This section is designated for those who are looking to gain experience in presenting scientific information, as well as for those who are developing effective scientific presentation skills.

3:30 Introduction (A. Brown and S. Mohammadhosseinpour)
3:35 P-2037 Arabian Primrose Mediated Green Synthesis of Silver Nanoparticles and Evaluation of Their Anti-oxidant, Anti-cancer, Anti-microbial and Catalytic Potential
Shruti Nindawat, University of Delhi, and Veena Agrawal
3:55 P-2062 Elicitation of Prenylated Stimulations on Stilbenoids in Cell Suspension Cultures of Peanut
Abbas Karouni, Arkansas State University, and Fabricio Medina-Bolivar
4:15 P-2044 Effects of Chemical Stimulations on Shikonin and Derivatives in In Vitro Turkish Echium italicum L. Hairy Root Culture
Veysel Süzerer, Bingöl University and Istanbul University, Melike Tepe, Devrim Atilla, Engin Tilkat, Tamer Özcan, and Yelda Özden Çiftçi 

IN VITRO ANIMAL CELL SCIENCES CONTRIBUTED PAPERS

Moderator:     Mae Ciancio, Midwestern University

5:00 pm – 6:00 pm Animal Contributed Paper Session Meeting Room 12
5:00 A-1003 The Revivability and Growth Potential of Primary Chicken Embryo Fibroblast Cells Stored Frozen In Liquid Nitrogen
Girish Sarma, Hygieia Biological Laboratories
5:15 A-1004 Fish In Vitro Biology: Another Piece of the Puzzle for the Aquaculture of New Zealand Native Species Chrysophrys auratus
Georgina C. Dowd, The New Zealand Institute for Plant and Food Research Limited, Gavril Chong, Lucy E. J. Lee, Susan N. Marshall, and Maren Wellenreuther 
5:30 A-1005 Cisplatin-induced Apoptosis Requires Functional p53 in a Neuroblastoma Cell Line
Alexander Cairo, Midwestern University, Kelly Keeler, and Kolla Kristjansdottir
5:45 A-1006 Translating Large-scale Commercial Cell Culture Technologies to the Production of Cell-based Meat
Liz Specht, The Good Food Institute

LATE BREAKING TOPICS/YOUNG INVESTIGATOR PRESENTATIONS IN PLANT BIOTECH

Moderators: Nagesh Sardesai, CORTEVA AgriscienceTM, Agriculture Division of DowDuPontTM, and Veena Agrawal, University of Delhi

5:00 pm – 6:00 pm Plant Contributed Paper Session Grand Salon F
5:00 Introduction (N. Sardesai and V. Agrawal)
5:05 P-1016 Recombinase Technology for Gene Stacking: From Microbes to Plant
James Thomson, USDA-ARS-CIG, Leyla Hathwaik, Upul Hathwaik, Troy Weeks, Nic Nottingham, and Roger Thilmony
5:30 P-1017 Improved Soybean Resistance to Potyviruses Through Genetic Engineering
Bin Tian, Kansas State University, Hyeonju Lee, Jessica Rupp, John P. Fellers, and Harold N. Trick
5:40 P-1018 GRF5, a Novel Regeneration Booster Gene that Improves Transformation of Monocot and Dicot Species
David Pacheco-Villalobos, KWS SAAT SE, Susana Martin-Ortigosa, Jixiang Kong, Wolfgang Koch, and Klaus Schmidt
5:50 P-1019 Organ-specific Expression of Dermaseptin B1-derivative Host Defense Peptide in Potato Tubers for Reduced Post-harvest Losses
Dmytro P. Yevtushenko, University of Lethbridge

MICROPROPAGATION

Moderator:     Maria M. Jenderek, USDA-ARS

5:00 pm – 6:00 pm Plant Contributed Paper Session Grand Salons G-J
5:00 P-1020 Different Conditions During Pinus radiata Somatic Embryogenesis Produce Different Hormonal Profiles in the Somatic Embryos and Plantlets Obtained
I. A. Montalbán, Neiker-Tecnalia, O. Novák, M. Strnad, T. Goicoa, M. D. Ugarte, and P. Moncaleán
5:15 P-1021 Improved Somatic Embryogenesis in Norway Spruce Using Temporary Immersion Bioreactors
U. Egertsdotter, Georgia Institute of Technology, N. H. A. Mamun, and C. K. Aidun
5:30 P-1022 Cryopreservation of In Vitro Cannabis sativa L. Nodal Segments Using Vitrification
Cassandra D. Downey, Canopy Growth Corporation, Gregory Golenia, Ekaterina Boudko, and Andrew Maxwell Phineas Jones
5:45 P-1023 Prolific Regeneration of Octoploid Strawberry via Indirect Somatic Embryogenesis from Tip Runner Tissue
Cheol-Min Yoo, University of Florida, Vance M. Whitaker, and Seonghee Lee
6:00 pm – 7:30 pm IN VITRO ANIMAL CELL SCIENCES SECTION BUSINESS MEETING Meeting Room 12

6:00 pm – 7:30 pm PLANT BIOTECHNOLOGY SECTION BUSINESS MEETING Meeting Rooms 8&9

7:30 pm – 10:00 pm JOINT SECTIONS’ SOCIAL Il Terrazzo

PANEL DISCUSSION: FUTURE DIRECTIONS IN DEVELOPMENT AND APPLICATIONS OF CELL LINES

Moderators:     John W. Harbell, JHarbell Consulting, LLC, and Jessica Monserrate, BASF

8:30 pm – 9:30 pm Panel Discussion Meeting Rooms 12

Speakers representing all In Vitro Animal Cell Sciences sessions will participate in a panel discussion to formulate strategies for the development and application of cell lines to meet future needs in the research community. Discussion points will include: historical perspective of the development of cell lines (lessons learned); how to induce/maintain differentiation in a variety of cell types (including the roles signaling molecules play); pros/cons of using 3D cultures; media development/optimization strategies; best practices for assessing cell phenotypes.

8:30 Introduction (J. W. Harbell and J. Monserrate)
8:35 Panel Discussion
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