Final Program

Interactive Poster Presentations can be found in the program below.
View the posters, virtual posters, and silent abstracts.

View the abstracts for the program.


SATURDAY, JUNE 4

7:00 am – 7:00 pmRegistration
Pacific Foyer
8:00 am – 12:00 pmSIVB Board of Directors Meeting
Palm 7
3:00 pm – 6:00 pmPoster Set-up
Pacific A
3:30 pm – 4:30 pm2022 Program Planning Committee Meeting
Palm 8
4:30 pm – 5:00 pmFinance Committee Meeting
Palm 7
7:00 pm – 9:00 pm2022 In Vitro Biology Meeting Welcome Reception
Pacific A
7:00 pm – 9:00 pmThe Mission Bay Silent Auction Kickoff
Pacific A
7:30 pm – 8:30 pmPoster Session
Even Poster Authors will be present
See list of posters
Pacific A
8:30 pm – 10:00 pm

DESIGN OF EXPERIMENTS WORKSHOP*

Pacific E
PLANT WORKSHOPConveners: Uyen Cao Chu, Corteva Agriscience, Randall P. Niedz, USDA, and Todd J. Jones, Corteva Agriscience
Read Session Description

Determining optimal formulations and conditions for improved in vitro growth is often done by testing one ingredient or set of conditions at a time. Design of Experiment (DoE) methodology is a particularly useful set of techniques that allows testing of multiple compounds, processes, or environmental conditions simultaneously in multiplex systems. The principles and statistics behind DoE are complex and require DOE software to design and analyze the experiments. In this workshop, the audience will get an introduction to the use of DOE principles and tools by participating in the design and data analysis of prepared examples relevant to various in vitro culture scenarios.

8:30Introduction (U. C. Chu and T. J. Jones)
8:35P-30DoE Methodology vs. “Standard Stats” – Software for DoE Methodology – DOE Principles and Types of Experiments
Randall P. Niedz, USDA
9:00PS-31DOE Experiment Walkthrough
Uyen Cao Chu, Corteva Agriscience
9:25Discussion

SUNDAY, JUNE 5

7:30am – 5:30 pmRegistrationPacific Foyer
7:00 am – 8:00 amIn Vitro Animal Cell Sciences Program Committee MeetingPacific H&I
8:00 am – 10:00 am

ADVANCED APPLICATIONS OF SITE DIRECTED NUCLEASES*

Pacific C&D
PLENARY
SYMPOSIUM
Conveners: Durga Attili, University of Michigan Medical School, Pierluigi Barone, Corteva Agriscience, Raj Deepika Chauhan, Pairwise, and Michael Dame, University of Michigan Medical School
Read Session Description

In the last two decades the development of different site directed nucleases (e.g. ZFNs, TALENs and CRISPR-Cas) has provided tools to precisely and efficiently introduce a variety of genetic modifications in a broad spectrum of cell types and organisms. The most recent deployment of these programmable nucleases is represented by new applications including CRISPR gene drive, targeted induction of chromosomal rearrangements and diagnostic screening for genetic mutations and development of nutritious, flavorful new varieties of fruits and vegetables. In this session a comprehensive review and exciting recent developments in this area will be presented.

8:00Introduction (D. Attili, P. Barone, R.D. Chauhan, and M. Dame)
8:05PS-1Applications of and Considerations for CRISPR/Cas9 Mediated Gene Conversion in Rodents
Kimberly Cooper, University of California, San Diego
8:35PS-2Chromosome Engineering for Crop Improvement
Sergei Svitashev, Corteva Agriscience
9:05
PS-3
CRISPR-Powered Microchips: A Paradigm Shift for Amplification-free DNA Detection
Kiana Aran, Keck Graduate Institute; Cardea Bio
9:35
PS-11
Application of Genome Editing to Improve Nutritious, Fresh Produce Crops for the Consumer
Ryan Rapp, Pairwise
10:00 am – 10:30 amCoffee BreakPacific A
10:00 am – 10:30 amPublic Policy Committee MeetingPalm 8
10:30 am – 12:30 pm

FRONTIERS IN GENE EDITING FOR CROP DEVELOPMENT*

Pacific C&D
PLANT SYMPOSIUMConveners: Fredy Altpeter, University of Florida – IFAS, and Yiping Qi, University of Maryland-College Park
Read Session Description

The development of innovative CRISPR/Cas gene editing technologies is refining the speed and precision of plant breeding as urgently needed to feed a growing population on a warming globe. Topics covered in this session will include the development of improved approaches and tools for delivery of gene editing reagents to crops, advances with the precision technologies base editing and prime editing supporting targeted nucleotide substitutions, as well as strategies and examples of gene editing and synthetic directed evolution for crop improvement.

10:30Introduction (F. Altpeter and Y Qi)
10:35P-1AEngineering the “Engineers”: Efforts in the Biolistic Gun Improvement and Agrobacterium Genome Engineering
Kan Wang, Iowa State University
11:00P-2Developing Highly Efficient Base Editing and Prime Editing Tools in Plants
Yiping Qi, University of Maryland – College Park
11:25
P-3
Gene Editing in Plants Using Plant Viruses
Savithramma Dinesh-Kumar, University of California – Davis
11:50
P-4
Transforming the Food System with the Inari SEEDesignTM Platform
Helene Berges, Inari Agriculture Inc.
12:15Discussion
P-1*Synthetic Directed Evolution in Plants: Unlocking Trait Engineering and Improvement
Magdy M. Mahfouz, KAUST, Saudi Arabia
*Presentation will be available online only
10:30 am – 12:30 pm

ADVANCES IN DOUBLE HAPLOID TECHNOLOGY

Pacific E
PLANT SYMPOSIUMConveners: Cliff Hunter, Consultant, and Charles L. Armstrong, Plastomics
Read Session Description

Doubled haploid technology is now a cornerstone of plant improvement programs for many of the world’s most important crops, including maize, wheat, oilseed rape, and a wide variety of vegetable / horticultural species. Significant, steady improvement has been made over the years in improving the rate of haploid induction, the identification and selection of haploids, and the efficiency of chromosome doubling and recovery of dihaploid seed. In recent years, important breakthroughs have been made in understanding the molecular basis of haploid induction. These include cloning of a major gene involved in haploid induction in maize and development of a novel chromosome elimination mechanism (modified CENH3), each of which has been demonstrated to work across species. Haploid induction has also been combined with gene editing to increase the speed with which edited traits can be fixed and evaluated in relevant germplasm. In this session, we will bring together representatives of several major seed companies to present selected highlights of recent work on dihaploid technology within their organizations.

10:30Introduction (C. Hunter and C. L. Armstrong)
10:35P-5Accelerating Precision Breeding through Double Haploids
Lorena Moeller, Bayer Crop Science
11:10P-6Breeding Technology & Discovery Progress at Syngenta
Weiguo Liu, Syngenta Crop Protection, LLC
11:45
P-7
Deciphering In Planta Haploidization In Maize
Nathanaël Jacquier, Limagrain
12:20Discussion
10:30 am – 12:30 pm

ORGANOID TECHNOLOGY

Pacific H&I
ANIMAL
SYMPOSIUM
Conveners: Terry Riss, Promega Corporation, and Kristina Martinez-Guryn, Midwestern University
Read Session Description

The use of organoids has grown rapidly over the past several years. As a 3D culture model system, they have been demonstrated to more closely represent in vivo biology compared to cells cultured as a monolayer on plastic. The technology to produce organoids has improved greatly, resulting in protocols to generate organoids representing a variety of tissue types. There are many definitions of organoids; but a key element is they are derived from stem cells or organ progenitors that are driven to differentiate into several cell types that self-organize similar to the process in vivo. The speakers in this Symposia will present a general overview of organoids and provide examples of their generation and practical applications to advance our understanding of biology.

10:30Introduction (T. Riss)
10:35A-1Fitting Organoids into the Spectrum of Available 3D Culture Models
Terry Riss, Promega Corporation
11:00A-2Introducing a ‘Phase 0’ in Clinical Trials with Precise Organoid-based Disease Models
Courtney Tindle, University of California, San Diego
11:25
A-3
Initiation, Expansion, and Cryopreservation of Patient-derived Organoids from the Human Cancer Models Initiative
James M. Clinton, American Type Culture Collection
11:50A-4Brain Organoid Technology: a Versatile Tool to Study the Human Brain
Francesca Puppo, University of California San Diego
12:15Discussion
11:00 am – 12:00 pmSIVB/IAPB/Springer MeetingPalm 7
12:30 pm – 1:30 pmExhibitors Refreshment BreakPacific A
12:30 pm – 1:30 pmMembership Committee MeetingPalm 8
12:30 pm – 1:30 pmIn Vitro – Plant Editorial Board MeetingPalm 7
1:30 pm – 3:00 pm

EXTRACELLULAR MATRIX AND TISSUE ENGINEERING

Pacific H&I
ANIMAL
SYMPOSIUM
Conveners: Joshua Z. Gasiorowski, Midwestern University, and Michael K. Dame, University of Michigan Medical School
Read Session Description

Tissue engineering has had exciting clinical successes, but new translational breakthroughs in the field will be dependent upon researchers fully understanding the properties of endogenous extracellular matrices so they can design biologically relevant mimics. The extracellular matrix (ECM) is a three-dimensional proteinaceous meshwork that provides tensile strength and connective, anchoring material for all tissues. However, the ECM is also a rich source of signals that influence and control cell behaviors. The biochemical composition of collagens, laminins, and other fibrous proteins act as ligands for cell signaling receptors while the biophysical cues in the form of compliance, elasticity, and topography directly mediate independent cellular responses. This session will highlight recent advances in understanding the basic science properties of the extracellular matrix, in addition to discussing ECM mimics that are designed to serve as tissue engineering scaffolds for regenerative medicine.

1:30Introduction (J. Z. Gasioiorowski and M. K. Dame)
1:35A-5Beyond Cultrex BME: Ultimatrix and Other Matrices for Stem Cells and Organoids
Kevin Flynn, Bio-Techne
2:15A-6Dentin Extracellular Matrix: Biological and Mechanical Properties for Bioinspired Tissue Engineering
Marcela R. O. Carrilho, Midwestern University
2:55

Discussion
1:30 pm – 3:00 pm

NOVEL DELIVERY TECHNOLOGIES – OVERCOMING BOTTLENECKS

Pacific E
PLANT SYMPOSIUMConvener: Gözde Demirer, California Institute of Technology
Read Session Description

The need to accelerate crop breeding programs has never been greater, as the world population is exponentially increasing, climate is changing, and resources are limited. Breeding relies on genetic variation, be it natural, induced, or introduced. Given the recent development and rapid advances in the field of gene editing through the CRISPR/Cas technology, plant genetic engineering has become a highly promising approach for increasing crop yields and nutritional value, and to generate high-yielding cultivars that are resilient to various biotic and abiotic stresses that can grow with less water and nutrient resources. Despite the recent progress in genome editing, most plant species remain impossible or difficult to genetically engineer, retarding the progress in plant biology research and crop improvement. The two most critical bottlenecks of generating engineered plants are i) biomolecule delivery into plant cells with walls and ii) tissue culture-based plant transformation. The common transformation tools, Agrobacterium and gene gun, are not able to transform plant reproductive organs and germ cells directly, except in a few model species, therefore necessitating regeneration of plants from the transformed explants. Plant regeneration in tissue culture is a low-throughput and laborious process that requires specialized methodology development for each species and genotype, and not all plants are amenable to tissue culture. Moreover, Agrobacterium itself is a highly species-dependent tool, limiting the extension of developed biotechnologies to a broad range of plants. Given these limitations, there is an immense need to develop broadly-applicable and innovative plant delivery and transformation technologies. In this session, we will discuss these new and novel delivery technologies to plants with impactful applications in agriculture and fundamental plant biology research.

1:30Introduction (G. Demirer)
1:35P-8Overcoming Bottlenecks that Prevent Efficient Foliar Uptake and Translocation of Polymer-based Nanoparticles in Crop Plants
Gregory Lowry, Carnegie Mellon University
2:00P-9TBD
Michael Maher, Invaio Sciences
2:25P-10
Nanomaterials for Plant Genetic Engineering
Gözde Demirer, California Institute of Technology
2:50Discussion
1:30 pm – 3:00 pm

PLANT GROWTH REGULATORS

Pacific F&G
PLANT SYMPOSIUMConvener: Geny Anthony, Corteva Agriscience, and Kristian Adamek, University of Guelph
Read Session Description

Plant Growth Regulators (PGRs) are fundamental to growth and development of plants at the cellular, tissue and organ levels. PGRs have the capacity to influence cell division, cell expansion, and cell structure and function, in addition to mediating environmental stress. Since the recognition of their functional importance in the 1930s, the main classes of PGRs (such as auxins, gibberellins, cytokinins, abscisic acid and ethylene) have been well researched. Apart from their use as growth enhancers in agriculture and horticulture, PGRs and their synthetic analogues have been extensively used for in vitro manipulation of plant tissues for research and commercial purposes. PGRs rarely act alone and are almost always involved in pathways where multiple PGRs interact. Recent advances in this field have unraveled their complex mechanisms and pathways. Plus, many new structurally similar and/or chemically unrelated compounds with PGR-like effects have been discovered. This session will inform the audience about the latest advances in understanding the complex mechanism and pathways of PGRs.

1:30Introduction (G. Anthony and K. Adamek)
1:35P-11Control Plant Growth by Modulating Auxin Biosynthesis and Degradation
Yunde Zhao, University of California, San Diego
2:15P-12ABA Receptors: Agonists, Antagonists, and Scaffolds for Biosensors Development
Sean Cutler, University of California, Riverside
2:55Discussion
3:10 pm – 5:30 pm

2022 IN VITRO BIOLOGY MEETING OPENING CEREMONY

Pacific C&D
KEYNOTE SYMPOSIUMConveners: Mae Ciancio, Midwestern University, and Addy Alt-Holland, Tufts University
3:10KS-1Welcome and Opening Remarks
Mae Ciancio, Midwestern University
Addy Alt-Holland, President, Society for In Vitro Biology
3:20ROBERT H. LAWRENCE, JR. KEYNOTE SYMPOSIUM
Introduction (J. W. Harbell)
KS-1Advancing Cell Culture to Meet Scientific and Societal Needs
Thomas Hartung, Director of the Center for Alternatives to Animal Testing (CAAT) and Inaugural Holder of the Doerenkamp-Zbinden
Endowed Chair in Evidence-based Toxicology in the Department of Environmental Health Sciences at the Johns Hopkins
Bloomberg School of Public Health
4:10Questions and Answers
4:202022 SOCIETY FOR IN VITRO BIOLOGY AWARDS CEREMONY
Addy Alt-Holland, President, Society for In Vitro Biology
2022 Lifetime Achievement Award Presentations
4:25(Introduction by Sandra L. Schneider, Acknowledgement by Dr. Pomponi to follow)
Shirley A. Pomponi, Harbor Branch Oceanographic Institute, Florida Atlantic University
4:40(Introduction by Wayne A. Parrott; Acknowledgement by Dr. Wang to follow)
Kan Wang, Iowa State University
4:55Acknowledgement of the 2020 and 2021 Lifetime Achievement Award Recipients
Dwight T. Tomes, 2020 Lifetime Achievement Award Recipient
Cynthia L. Goodman, 2021 Lifetime Achievement Award Recipient
5:102022 Fellow Award Recipients (Awards to be presented at Section Meetings)
Mae Ciancio, Midwestern University
Maria M. Jenderek, USDA
Kolbrun Kristjansdottir, Midwestern University
J. Pon Samuel, Corteva Agriscience
5:152022 Distinguished Service Award Presentations
(Awards to be presented by Allan R. Wenck):
Ad Hoc Webinar Committee
Rakhi Chaturvedi, Indian Institute of Technology-Guwahati
Ian Scott Curtis,
Consultant
Ahmad Al-Sayed Omar
, University of Florida
Brad L. Upham, Michigan State University
Ad Hoc Social Engagement Committee
Christopher Bagley, INARI
Anissa Belfemi, Harvard University
Sarbesh Das Dangol,
Tribhuvan University

Grant Support
Sadanand A. Dhekney, University of Maryland, Eastern Shore

Outgoing Board of Directors
John W. Harbell, JHarbell Consulting
Sukhpreet Sandhu, HMClause
Kan Wang, Iowa State University
5:25Acknowledgement of the 2020 and 2021 Distinguished Service Award Presentations
Barbara B. Doonan, New York Medical College
John J. Finer, The Ohio State University
Sukhpreet Sandhu
, HMClause
Dwight T. Tomes
Brad L. Upham
, Michigan State University
Harold N. Trick
, Kansas State University
Kan Wang
, Iowa State University
Allan R. Wenck, Syngenta Crop Protection LLC
2021 Distinguished Service Award Recipients
Addy Alt-Holland, Tufts University
Pierluigi Barone, Corteva Agriscience
Barbara B. Doonan,New York Medical College
Raj Deepika Chauhan, Pairwise
John W. Harbell, JHarbell Consulting, LLC
Evan M. Hill, University of Michigan
Max Jones, University of Guelph
Angela Labrum, Bailey Nursery
Kristina Martinez-Guryn, Midwestern University
Alperen Ozturk, Nigde Omer Halisdemir University
Wayne A. Parrott, University of Georgia
M. Annie Saltarikos, Bayer U.S. – Crop Science
Sukhpreet Sandhu,
HMClause
Marietta Wheaton Saunders
, New Beginnings Management, Inc.
Michele G. Schultz, New Beginnings Management, Inc.
Brad L. Upham, Michigan State University
5:30Adjourn
Group photo with Dr. Hartung and Student attendees
5:30 pm – 6:30 pm
2022 IN VITRO BIOLOGY MEETING OPENING CEREMONY RECEPTIONPacific A
6:30 pm – 7:30 pmPoster Session
Odd Poster Authors will be present.
See list of posters
Pacific A
7:30 pm – 9:30 pm

STUDENT WORKSHOP: STATE-OF-THE-ART CELL IMAGING TECHNOLOGIES

Pacific F&G/Pacific A
EDUCATION WORKSHOPMubeen ul Hasan, Nigde Omer Halisdemir University, Muneeb Hassan Hashmi, Nigde Omer Halisdemir University and Brad Upham, Michigan State University
Read Session Description

Since the earliest microscopy efforts in the 1600s, cell imaging has significantly modernized and became a ubiquitous tool in cell biology laboratories. Light microscopy was the earliest technique used in biology and has remained a main staple of cell biology and continues to evolve into more advanced techniques, which has been fueled by developments in computation, image detection devices, labelling, and sample preparation strategies. This student-sponsored workshop is to provide hands-on and virtual experiences in some of the latest imaging technologies.

7:30Introduction (M ul Hasan and M. H. Hashmi)
7:35Panel Discussion
Panelists:
Addy Alt-Holland, Tufts University
Michael J. Fay, Midwestern University
Brad L. Upham, Michigan State University
Kan Wang, Iowa State University

MONDAY, JUNE 6

7:00 am – 6:00 pmRegistrationPacific Foyer
7:00 am – 8:00 amPlant Biotechnology Program Committee MeetingPacific F&G
7:00 am – 8:00 amPublications Committee MeetingPalm 7
8:00 am – 10:00 am

MICHAEL E. HORN EMERGING TECHNOLOGIES SYMPOSIUM: FUTURE FOODS

Pacific C&D
PLENARY
SYMPOSIUM
Vivian R. Dayeh, University of Waterloo, Veena Veena, Donald Danforth Plant Science Center, and Sukhpreet Sandhu, HMClause
Read Session Description

Meats grown in a petridish, chocolate produced without cacao trees, and milk produced without ever stepping on a farm. Is this the future of the foods we eat? Increasing threats to world’s food production systems and the need to creating sustainable food products and reducing carbon emissions from agriculture, has been driving the growth of plant based and cell based foods. One of these emerging areas is cellular agriculture that relies on in vitro methods instead of whole plants or animals for food production. The Future Foods plenary session will present cutting edge research on alternative food sources including plant and animal cell cultures and how the technology will change the food system in the future. The speakers in this symposium will excite the audience on the future of alternative seafoods, chocolate, and milk products, and how these innovations will revolutionize agriculture.

8:00Announcement of the Michael E. Horn Emerging Technologies Symposium
Addy Alt-Holland, President, Society for In Vitro Biology
8:05Introduction (V. R. Dayeh, V. Veena, and S. Sandhu)
8:10PS-4Protein Reimagined: Transforming the Global Food System
Bruce Friedrich, The Good Food Institute
8:35PS-5Plant-based and Cell-cultured Seafood for a More Sustainable Future and Healthier Ocean
Brandon Chen, Finless Foods
9:00
PS-6
Leveraging Bioprocessing and Fermentation Technologies for the Generation of Cell-based Milk and Bioactive Ingredients  
Aletta Schnitzler, Turtle Tree
9:25
PS-7
The Fall of Specialty Monoculture Farming, and the Rise of Cell Cultured Foods
Alan Perlstein, California Cultured
9:50Discussion
10:00 am – 10:30 amCoffee BreakPacific A
10:00 am – 10:30 amAwards Committee MeetingPalm 7
10:30 am – 12:30 pm

MOVING THE FIELD FORWARD: APPLICATION OF NEW TECHNOLOGIES IN ACADEMIC, INDUSTRY AND REGULATORY AGENCIES PERSPECTIVES

Pacific H&I
ANIMAL
SYMPOSIUM
Conveners: Addy Alt-Holland, Tufts University, Kenneth Kandaras, International Foundation for Ethical Research, and John W. Harbell, JHarbell Consulting LLC
Read Session Description

Academic institutions are centers not only for learning but also for innovation that varies from highly exploratory framework to a more commercially aligned approach. With increasing frequency, academic researchers are interested in moving their ”basic” research program discoveries along the innovation pathway to commercial translation and development of new technologies for the benefit of humankind. The original basis of the biopharmaceutical industry, 3-D bioengineered tissue models, and organs-on-a-chip technologies are only a few examples of this transition, which started as basic science questions that grew into applied technologies. However, although numerous invention disclosures, intellectual property, and patents are generated in academic institutions, they are not always translated to licenses and spin-outs of successful start-up companies. The translation of research to commercialized innovations is often hindered by multiple factors, such as professional motivations and interest, knowledge of the innovation process, and expectations of outcomes. Moreover, the gap from conceiving research-based ideas in the academic arena to their translation into industry-based, innovative and applicable products in the healthcare settings consists of multiple barriers. These span from inadequate design of research programs, to insufficient marketing of the ideas, to technical development and manufacturing (including the reliability, intended objective efficacy and predictive capacity of the innovative end product), to complex regulatory procedures, approvals and policies that can influence the acceptance and commercial viability of new technologies. As moving from research idea towards a commercial product is a complex process, innovation in healthcare requires multidisciplinary teams that are based on engaged stakeholders from both academic and industry. Do you have a technology that you feel is worth commercializing? This symposium will examine the factors that influence moving the innovation filed forward through the experiences of three speakers from organizations that are bringing new technologies to the scientific academic, industrial, and regulatory communities. Our speakers will discuss their experience, challenges and perspectives on the translation of research to commercialized innovations, how academic motivations and work practices can shape successful research translation, as well as the characteristics and expectations involved in healthcare innovation in industry.

10:30Introduction (J. W. Harbell, K. Kandaras and A. Alt-Holland)
10:35A-7From Assay to Products – Lessons Learned
Thomas Hartung, Center for Alternatives to Animal Testing
11:10A-8From Lab to Product: Commercialization Paths for Medical Diagnostic Tests
Hadley Sikes, Massachusetts Institute of Technology
11:45A-9From Academia to Industry – the Importance of Good Laboratory Practices Guidelines for Developing Consistent Study Execution
John W. Harbell, JHarbell Consulting LLC
12:20Discussion
10:30 AM –12:10 PM

PLANT BIOTECHNOLOGY POST-DOCTORAL ORAL PRESENTATION COMPETITION

Pacific E
PLANT CONTRIBUTED
PAPER SESSION
Moderator: Ahmad Al-Sayed Omar, University of Florida
Panel of Plant Biotechnology Experts Evaluating the Contestants: Pal Maliga, Rutgers University, Carlos M. Hernandez-Garcia, CTC Gemonics, and Terrence Frett, Sun World Innovations
Read Session Description

To support the Society’s vision to encourage education and scientific informational exchange and recognize outstanding post docs, the Plant Biotechnology Section is pleased to announce the 2022 Post-Doctoral Oral Presentation Competition. Competition finalists were selected based on the quality of the abstracts. A panel of judges will evaluate the presentations at the meeting. Criteria for the evaluation include experimental design, data analysis, proper interpretation of the results, originality of the study, technical difficulty, appearance and ability of the Post-Doctoral candidate to present it. Winners will be presented with a certificate and a cash award at the meeting.

10:30P-1000Field Evaluation of Metabolically Engineered Energycane for Hyperaccumulation of Triacylglycerol
Viet Dang Cao, DOE Center for Advanced Bioenergy and Bioproducts Innovation, Baskaran Kannan, Shelby Korynta, Hui Liu, Steve Long, John Shanklin, and Fredy Altpeter
10:50P-1001Author is unable to attend.
11:10P-1002Optimizing CRISPR/Cas9-mediated Genome Editing in Vitis Papaiah Sardaru, University of Maryland Eastern Shore
C. Jackson, A. Junior, B. Khatabi, X. Dai, Y. Zhao and S. A. Dhekney
11:30P-1003Comparison of Constitutive Promoters for Spatial Regulation of Transgenes in Different Vegetative Tissues of Sugarcane
Rajesh Yarra, University of Florida, Evelyn Zuniga-Soto, and Fredy Altpeter
11:50P-1004Author is unable to attend.
10:30 am – 12:30 pm

PLANT BIOTECHNOLOGY STUDENT ORAL PRESENTATION COMPETITION

Pacific C&D
PLANT CONTRIBUTED
PAPER SESSION
Moderator:      Alex da Silva Conceicao, Calyxt
Panel of Plant Biotechnology Experts Evaluating the Contestants: Max Jones, University of Guelph, Satya Swathi Nadakuduti, University of Florida, Jeff Beringer, Inari Agriculture, and Uyen Cao Chu, Corteva Agriscience
Read Session Description

To support the Society’s vision to encourage education and scientific informational exchange, the Plant Biotechnology Section is pleased to announce the 2022 Student Oral Presentation Competition. Student competition finalists were selected based on the quality of the abstracts. A panel of judges will evaluate the presentations at the meeting. Criteria for the evaluation include experimental design, data analysis, proper interpretation of the results, originality of the study, technical difficulty, appearance and ability of the student candidate to present it. Winners will be presented with a certificate and a cash award at the meeting.

10:30

P-1005

Highly Conserved sgRNA Target Sequences Support Cas9-mediated Mutagenesis of LIGULELESS1 in Both Sorghum and Sugarcane
Eleanor J. Brant, University of Florida, Ayman Eid, Mehmet Cengiz Baloglu, Aalap Parikh, and Fredy Altpeter

10:50

P-1006

Knock-down of Vital Gene(s) of Tuta absoluta (Meyrick) (Lepidoptra: Gelechiidae) Using in Planta RNAi
M. H. Hashmi, Niğde Ömer Halisdemir University, U. Demirel, and A. Bakhsh

11:10

P-2000

Comparative Assessment of Antioxidant Activity of Prenylated Stilbenoid-Rich Extracts from Elicited Hairy Root Cultures of Three Different Cultivar of Peanut

G. Gajurel, Arkansas Biosciences Institute, R. Hasan, and F. Medina-Bolivar

11:30

P-1008

Efficient, Multi-allelic Editing for the Genetic Improvement of Bahiagrass (Paspalum notatum Flüggé)

David May, University of Florida, Jennifer Gilby, Sara Sanchez, and Fredy Altpeter

11:50

P-1009

Targeted Mutagenesis of Vacuolar H+ Translocating Pyrophosphatase (V-PPase) Promoter Limits Sucrose Formation and Disturbs Cytosolic pH During Germination in Rice

D. Dharwadker, University of Arkansas, P. J. I. Gann, C. Maurya, S. Nandy, S. Zhao and V. Srivastava

12:10

P-1010

Deletion in the GATA Promoter Element of Vacuolar H+ Translocating Pyrophosphatase (V-PPase) by CRISPR/Cas9 Reduces Chalkiness in Rice

Peter James Icalia Gann, University of Arkansas, Dominic Dharwadker, Chandan Mayura, Soumen Nandy, Shan Zao, and Vibha Srivastava

12:30 pm – 1:30 pmLong Range Planning Committee MeetingPalm 7
12:30 pm – 1:30 pmRefreshment BreakPacific A
12:30 pm – 1:30 pm

STUDENT NETWORKING LUNCHEON: RESEARCH PROPOSAL AND GRANT WRITING

Pacific F&G
EDUCATION WORKSHOPConveners:    Mubeen ul Hasan, Nigde Omer Halisdemir University, Muneeb Hassan Hashmi, Nigde Omer Halisdemir University, and Brad L. Upham, Michigan State University
Read Session Description

Have you been looking for how to write a successful research proposal and how to get funding for your research as a graduate student? Have you been searching for how to find the right funding agency for your research or even thought about writing a proposal that will be accepted by your funding agency or your supervisor? Or are you confused about how to target the key areas of a topic that will make your research proposal interesting for the jury and funding agency? If that’s the case, this is your golden opportunity! Come to the student networking luncheon to find all the answers you have been waiting for. We are putting together a panel of researchers that will answer all of your questions and will help alleviate any confusion. If you are a new Master’s or Ph.D. student who needs to write a proposal, then researchers will be available to assist you and provide you with information about how to find the perfect topic for your research as well as all the material that you need to write a proposal. If you are a student and you need to find a public or private funding agency to fund your research, our professionals will help you understand the requirements of the various funding agencies. Similarly, if you want to apply for a scholarship to study abroad and your scholarship requires a strong research proposal, this panel will tell you how they chose students for these positions which can help you get answers to your questions. Professionals will also share their academic experiences and discuss how they coped while pursuing their graduate degrees. This workshop may also introduce you to your new research mentor, supervisor, collaborator, and/or external internship opportunity.

12:30Introduction (M ul Hasan and M. H. Hashmi)
12:35Discussion
1:30 pm – 2:30 pm

IN VITRO ANIMAL CELL SCIENCES STUDENT AND POST DOCTORAL ORAL PRESENTATION COMPETITION

 Pacific H&I
ANIMAL CONTRIBUTED
PAPER SESSION
Moderators:     Addy Alt-Holland, Tufts University, and Kolla Kristjansdottir, Midwestern University
Panel of In Vitro Animal Cell Sciences Experts Evaluating the Contestants: Addy Alt-Holland, Tufts University, Kolla Kristjansdottir, Midwestern University, John W. Harbell, JHarbell Consulting, LLC, Barbara Doonan, New York Medical College, Michael J. Fay, Midwestern University, Brad L. Upham, Michigan State University, Michael Dame, University of Michigan, Mae Ciancio, Midwestern University, Anissa Belfetmi, Harvard University, and Zoe Xiaofang Zhu, Tufts University
Read Session Description

The In Vitro Animal Cell Sciences Section (IVACS) of the Society for In Vitro Biology is pleased to announce the 2022 Student and Post-Doctoral Oral Presentation Competition during the SIVB meeting in San Diego, CA. This competition encourages the exchange of scientific information between the presenters, attendees and judges. Additionally, it provides an invaluable opportunity for students and post-docs to practice and improve their presentation delivery and public speaking skills. The oral presentations will be discussed and graded by a panel of expert judges. Evaluation criteria will include experimental design, data analysis, proper interpretation of the results, originality of the study, technical difficulty, professionalism and ability of the finalist to explain the research and answer questions, and importantly, adherence to the allocated time for the presentation. The Student and Post-Doctoral Oral Presentation Competition session serves to recognize and reward the research and achievements of outstanding students and post-docs. The three finalists will be presented with a certificate and a cash award during the meeting.

1:30

A-1000

Habitat Restoration and Production of Bioactive Compounds from 3D Sponge Cell Cultures

Megan Conkling, Harbor Branch Oceanographic Institute, Elizabeth Urban-Gedamke, Amy E. Wright, and Shirley A. Pomponi

1:50

A-1001

Assessing the Apoptosis Effect of Prenylated Stilbenoids Combined with Paclitaxel in Triple-negative Breast Cancer Cells

Sepideh Mohammadhosseinpour, Arkansas State University, A. Weaver, L-C. Ho, and F. Medina-Bolivar

2:10

A-1002

A Flavonoid-rich Extract of Moringa oleifera (Moringaceae) Leaf Cultivated in Brazil Inhibited Inflammatory Mediators in Lipopolysaccharide-treated Macrophages

Larissa Marina Pereira Silva, North Carolina State University, Jade Schlam, Leandro Ferreira, Silvana Zucolotto, and Debora Esposito

1:30 pm – 2:30 pm

BIOTECHNOLOGY

Pacific A
PLANT INTERACTIVE
POSTER SESSION
Moderator:      Baskaran Kannan, University of Florida
P-2000Comparative Assessment of Antioxidant Activity of Prenylated Stilbenoid-Rich Extracts from Elicited Hairy Root Cultures of Three Different Cultivar of Peanut
G. Gajurel, Arkansas Biosciences Institute, R. Hasan, and F. Medina-Bolivar
P-2001In Vitro Selection for Fusaric Acid Resistant Egyptian Cumin Plants (Cuminum cyminum L.).
Hossam M. Kamel, Minia University, Kasem Z. Ahmed, and Mohamed K. A. Aly
P-2002RNAi Mediated Suppression of Flowering in Energycane to Enhance Biocontainment.
Baskaran Kannan, University of Florida, Thaibinhduong Nguyen, Niki Koukoulidis, Qasim Ali, Frico Situmeang, and Fredy Altpeter
1:30 pm – 2:30 pm

BIOTECHNOLOGY, GENOMICS, AND PLANT PHYSIOLOGY

Pacific A
PLANT INTERACTIVE
POSTER SESSION
Moderator: Savanah Senn, Los Angeles Pierce College

P-3000

A Rapid and Simplified Transformation and Genome Editing Method for Maize Inbred B104 Using Agrobacterium Ternary Vector System and Immature Embryos

Minjeong Kang, Iowa State University, Keunsub Lee, Todd Finley, Hal Chappell, Veena Veena, and Kan Wang

P-3001

CRISPR/Cas9 Mediated Cell Wall Engineering of Plant Cells for Enhanced Recombinant Protein Production

Uddhab Karki, Arkansas State University, Hong Fang, and Jianfeng Xu

P-3002

Post-wildfire Soil Microbiome Analysis of eDNA from the Angeles National Forest

Savanah Senn, Los Angeles Pierce College, Gerald Presley, and Sharmodeep Bhattacharyya

P-3003

Advanced Plant Reporter Genes for Transient Expression

Nathan Vorodi, Pennsylvania State University, Natalie Thompson, David Samson, Rekha Kandaswamy, Vijay Sheri, Aliya F. Anwar, Michael Ream, Anna Filipkowski, Maia Clipsham, Sairam Rudrabhatla, and Wayne R. Curtis

P-3004

Establishment of an Axenic Whitefly Colony for In Vitro Virus Transmission Studies

Wayne R. Curtis, The Pennsylvania State University, Natalie Thompson, David Krum, Yun-Ru Chen, Mariela Torres, Marena Trauger, Dalton Strike, Zach Weston, April Hile, and Jane Polston

P-3005

Genome-wide Analysis of Alpha-amylase Gene Family in Major Cereal Crops

Shabda Verma, Punjab Agricultural University, Kanwardeep S. Rawale, Navraj Kaur Sarao, Johar Singh Saini, Gagandeep Singla, and Kulvinder S Gill

2:30 pm – 3:30 pmPoster Session
Even Poster Authors will be present.
See list of posters
Pacific A
3:30 pm – 5:00 pm

ALTERNATIVE APPROACHES TO PLASTID ENGINEERING

Pacific E
PLANT SYMPOSIUMConvener:    Jeffrey Staub, Plastomics
Read Session Description

Stable plastid (chloroplast) transformation technology was developed more than 30 years ago. The technology has been used extensively to study plastid biology and to introduce a variety of traits with benefits to growers and consumers alike. However, only a limited number of model plant species are routinely transformed and no plants carrying engineered plastids have yet been commercialized, in part due to complexities of the technology and bottlenecks in tissue culture of agronomically important crops. More recently, alternative technologies are emerging as potential ways to engineer plastids that circumvent some of the previous challenges. This session will highlight some of those emerging technologies for plastid engineering and will discuss novel traits developed using those technologies.

3:30

Introduction (J. Staub)

3:35

P-13

Agrobacterium-mediated Transformation of the Plastid Genome

Pal Maliga, Rutgers University

4:00

P-14

Modification of the Plastid Genome Using Base Editing

Shin-Ichi Arimura, The University of Tokyo

4:25

P-15

Targeted Delivery of Plasmid DNA to Chloroplasts by Nanomaterials

Juan Pablo Giraldo, University of California Riverside

4:50

Discussion

3:30 pm – 5:00 pm

NEW APPROACHES TO VACCINE DEVELOPMENT

Pacific C&D
JOINT SYMPOSIUMConveners:     Julie Swartzendruber, Midwestern University, Barbara B. Doonan, New York Medical College, and J. Pon Samuel, Corteva Agriscience
Read Session Description

This joint symposium will focus on new developments and issues related to the use of vaccines. The rapid development and deployment of the COVID-19 vaccine has generated a lot of interest around how vaccines are developed. This session will invite expert faculty to discuss the latest information on vaccines including the future of vaccine development and surveillance

3:30

 

Introduction (J. Swartzendruber, B. B. Doonan and J. P. Samuel)

3:35

J-1

Plant-made Vaccines and Therapeutics for COVID-19

Henry Daniell, University of Pennsylvania

4:15

J-2

Biotechnology for the Next Generation of Veterinary Antiviral Vaccines

Hiep Vu, Nebraska Center for Virology

4:55

 

Discussion

3:30 pm – 5:00 pm

NON-COMPETITIVE STUDENT ORAL PRESENTATIONS

Pacific F&G
EDUCATION SYMPOSIUMModerators:  Mubeen ul Hasan, Nigde Omer Halisdemir University, and Muneeb Hassan Hashmi, Nigde Omer Halisdemir University
Read Session Description

The Society for In Vitro Biology (SIVB) takes pride in showcasing some of the most innovative research topics in both plant and animal sciences. Our mission for the non-competitive oral presentations is to provide a platform for student researchers to achieve recognition and share their research work with their peers as well as academic and industry professionals, who can take their projects to the next level without the pressure of competing. This session is designated for those who are looking to gain experience in presenting scientific information, as well as for those who are developing effective scientific presentation skills.

3:30

Introduction (M. ul Hasan, and M.H. Hashmi)

3:35

A-2007

Novel Mammalian Fibroblast Cell Culture Media Technique for Ultraviolet Cell Reduction
J. Hoeger, University of Dubuque

3:55

A-3013

Sex linked Aberrant Behavior and Hippocampal Gene
Expression in Egr1 Conditional Knockout Mice
C. Swilley, Virginia-Maryland College of Veterinary Medicine, and H. Xie

4:15

P-3013

Inducible Expression for Acceleration of Design-Build-Test-Learn Cycles in the Metabolic Engineering of Oilcane
Moni Qiande, University of Florida – IFAS, Dang Viet Cao, Hui Liu, John Shanklin, and Fredy Altpeter

4:35

A-3007

Cell Cycle Analysis of Vismodegib-treated Human Basal Cell Carcinoma Cells In-vitro
B. Senfi, Tufts University School of Dental Medicine, T. Mendez, M. Evers, S. Pagni, J. Cowan, and A. Alt-Holland

4:55

Discussion

5:00 pm – 6:00 pm

IN VITRO ANIMAL CELL SCIENCES CONTRIBUTED PAPERS

Pacific H&I
ANIMAL CONTRIBUTED
PAPER SESSION
Moderator:      Mae Ciancio, Midwestern University

5:00

A-1003

Successful Scale Up of Marine Sponge Cell Cultures in 3D Bioprinted Hydrogel Microdroplets

Elizabeth H. Urban-Gedamke, Harbor Branch Oceanographic Institute, Megan Conkling, and Shirley A. Pomponi

5:12

A-1004

Antimicrobial Studies of 1,3-Diphenylpyrazole-derived Anilines Against Methicillin-resistant Staphylococcus aureus

Hansa Raj KC, Arkansas State University, David Gilmore, and Mohammad A. Alam

5:24

A-1005

Moderate Heat Assisted Electrotransfer as an Effective Means for Delivering Molecules to Cells and Tissue

Richard Heller, University of South Florida, Jody Synowiec, Samantha Mannarino, Julie Singh, and Guilan Shi

5:36

A-1006

LncRNA APDC, a Long Non-coding RNA, Plays Important Regulatory Roles in Metabolism of Bone and Adipose Tissues

Zoe Zhu, Tufts University School of Dental Medicine, Yao Liu, Elissa K. Zboinski and Jake Chen

5:48

A-1007

Developing Cell Lines from Fish and Shellfish – Cellular Aquaculture Invitromatics

Lucy E. J. Lee, University of the Fraser Valley

5:00 pm – 6:00 pm

BIOTECHNOLOGY

Pacific C&D
PLANT CONTRIBUTED
PAPER SESSION
Moderator:     Nagesh Sardesai, Corteva Agriscience

5:00

P-1011

Somatic Embryogenesis in Cell Suspension Cultures of Sweetpotato (Ipomoea batatas)

Kedong Da, North Carolina State University, H. Leng, W. Liu, and G. C. Yencho

5:15

P-1012

Agrobacterium-mediated Genetic Transformation of Rose Embryogenic Cell Suspension Cultures

Kedong Da, North Carolina State University, H. Leng, D. Harmon, A. Nelson, N. Maren, W. Liu, and T. Ranney

5:30

P-1013

Identification and Validation of an Embryogenic Tissue Culture Response Gene from Maize Inbred Line A188 That Induces Embryogenic Callus Formation and Somatic Embryogenesis in Recalcitrant Maize Inbred B73

Frank McFarland, University of Wisconsin, Ray Collier, Kaitlyn Vondracek, Dalia Macias Martinez, and Heidi Kaeppler

5:45

P-1014

Reproduction Control Tools for Eucalyptus: Knockout of Flowering and Meiosis Genes Using CRISPR/Cas9 Mitigates Concerns about Gene Dispersal While Maintaining Normal Vegetative Development

Steven H, Strauss, Oregon State University, Michael F. Nagle, Surbhi Nahata, Bahiya Zahl, Alexa Niño de Rivera, Xavier V. Tacker, Estefania Elorriaga, Cathleen Ma, Greg Goralogia, Amy Klocko, Ellis Kline, and Michael Gordon
5:00 pm – 6:00 pm

BIOTECHNOLOGY, GENOME EDITING, AND GENETIC ENGINEERING

Pacific C&D
PLANT CONTRIBUTED
PAPER SESSION
Moderator:      Chhandak Basu, California State University, Northridge

5:00

P-1015

Molecular Physiological Responses of Paulownia and Turmeric Under Various Abiotic Stress Conditions

C. Basu, California State University, Northridge, M. Chaires, K. Cooper, D. Gupta, N. Joshee, N. Katiyar, S. Pakala, N. Ramadoss, B. Bharadwaj, and K. Musaev

5:15

P-1016

DNA-Free Genome Editing in Hexaploid Sweetpotato Directed by Preassembled CRISPR-Cas9 Ribonucleoprotein Complexes

Adrianne Brown, Tuskegee University Plant Biotechnology and Genomics Research Laboratory, M. Egnin, F. Bukari, D. Mortley, C. Bonsi, O. Idehen, D. Alexander, and G. Bernard

5:30

P-1017

Constitutive Expression of a miR169 Gene Alters Plant Development and Enhances Drought and Salt Tolerance in Transgenic Creeping Bentgrass

Xiaotong Chen, Clemson University, Jason Yeung, Andrew Fiorentino, Qian Hu, Morgan Kuess, and Hong Luo

5:45

P-1018

T-DNA-Free Gene Editing Through Transient Suppression of the POLQ Gene in Plants

Heqiang Huo, University of Florida, Guiluan Wang, and Zhanao Deng

6:00 pm – 7:30 pmIn Vitro Animal Cell Sciences Section Business MeetingPacific H&I
6:00 pm – 7:30 pmPlant Biotechnology Section Business MeetingPacific F&G
7:30 pm – 10:00 pmJoint Sections’ SocialPacific Foyer and Flamingo Lawn
8:30 pm – 10:00 pm

PUBLIC-PRIVATE COLLABORATIONS THAT SPUR INNOVATION WORKSHOP

Pacific C&D
PLANT WORKSHOPModerators: Sukhpreet Sandhu, HMClause, and Kathy Munkvold, Corteva Agriscience
Read Session Description

The global scientific community can do truly innovative work when we collaborate. Public-private partnerships (PPPs) between government agencies, universities, and other public research institutions with private sector companies fuel innovation by bringing unique capabilities and diverse thinking together to solve important challenges. Through common objectives, shared benefits and complementarity of human and financial resources, PPPs can connect the dots between a technology or solution to a problem and deploying that solution at scale. PPPs are increasingly used (in ag and biotech innovation) to enhance efficiency and accelerate innovation while fostering wider and faster uptake by leveraging public funds, mobilizing policy or government action. However, the success rate of many PPPs is variable with some failing to deliver the expected value both for the partners and the stakeholders. In this workshop, we will dive into common pitfalls for PPPs, risk management, best practices and guidelines on how to build PPPs for sustainable innovation.

8:30Introduction (S. Sandhu and K. Munkvold)
8:35Panel Discussion
Panelists:
Sukhpreet Sandhu, HMClause
Kathy Munkvold, Corteva Agriscience
Barbara Mazur, Pontifax AgTech
David D. Songstad, Songstad Consulting LLC
Brigitte Weston, Gates Ag One
Joyce Van Eck, Boyce Thompson Institute

TUESDAY, JUNE 7

7:00 am – 5:00 pmRegistrationPacific Foyer
7:00 am – 8:00 amIn Vitro – Animal Editorial Board MeetingPalm 7
7:00 am – 8:00 amStudent Affairs BreakfastPacific F&G
8:00 am – 10:00 am

ARTIFICIAL INTELLIGENCE AND MACHINE LEARNING IN PLANT AND ANIMAL SCIENCES

Pacific C&D
PLENARY SYMPOSIUMConveners: Mae Ciancio, Midwestern University, M. Annie Saltarikos, Bayer U.S. – Crop Science, and Evan M. Hill, University of Michigan
Student Convener: Babak Senfi, Tufts University
Read Session Description

Employing emergent technologies to optimize both human and plant health are important to the progress of our society and the world. Artificial intelligence and machine learning are changing the way scientists diagnose disease, identify effective drug modalities, analyze large datasets, and report outcomes. In this session, leaders in the field of AI and machine learning with specific interests in cancer, pharmacology, and crop sustainability will discuss current and ongoing efforts in this very exciting and dynamic field of investigation.

8:00PS-8The State of the Art of Artificial Intelligence in Healthcare
Aziz Nazha, Sidney Kimmel Cancer Center, Thomas Jefferson University
8:40PS-9Transfer Learning in Plant Biology
William Link, Bayer U.S. – Crop Science
9:20PS-10TBD
Gabriel A. Silva, University of California San Diego
10:00 am – 10:30 amCoffee BreakPacific A
10:00 am – 10:30 amEducation Committee MeetingPalm 7
10:30 amClosing of the Mission Bay Silent AuctionPacific A
10:30 am – 12:30 pm

ADVANCES IN CANNABIS BIOTECHNOLOGY

Pacific C&D
PLANT SYMPOSIUMConvener: Max Jones, University of Guelph
Read Session Description

The application of tissue culture and biotechnology is becoming a vital component of Cannabis businesses, especially as the market grows globally. Recent studies have found that plant pathogens have led to billions of dollars in damages to the Cannabis industry in North America, and advances in Cannabis Biotechnology have been identified as a potential solution to some of these problems. Additionally, the industry has had to innovate and draw from research and academia to fulfill the skyrocketing demand for healthy plants. This session brings together industry and academic researchers who are actively attempting to understand the challenges faced by current Cannabis businesses and the gaps that remain in our understanding of the horticulture and biology of Cannabis in the modern context. We hope to present information on treating pathogens (especially hop latent viroid), advances to Cannabis tissue culture techniques and advances in acclimatization of lab-grown plants back to greenhouses to be ready for cultivation.

10:30Introduction (M. Jones)
10:35P-16Controlled Ex Vitro Environments to Root Cannabis Microcuttings Following Multiple Harvest in a Hedging System
Jeffrey Adelberg, Clemson University
11:00P-17TBD
Hope Jones, Emergent Crop Sciences (ECS)
11:25P-18Advances in Cannabis Biotechnology- innovations in Cleaning, Screening, and Germplasm Maintenance
Chris Leavitt, Node Labs
11:50P-19Cannabis Virology in 2022 – Diagnostic and Remediation Options
Joseph Ramahi, Apikal Biotek
12:15Discussion
10:30 am – 12:30 pm

THE ART AND SCIENCE OF CELL IMAGING

Pacific H&I
ANIMAL SYMPOSIUMConveners: Brad L. Upham, Michigan State University, Debora Esposito, North Carolina State University, and Cynthia L. Goodman, USDA/ARS/BCIRL
Student Conveners: Muneeb Hassan Hashmi, Nigde Omer Halisdemir University and Mubeen ul Hasan, Nigde Omer Halisdemir University
Read Session Description

Since the earliest microscopy efforts in the 1600s, cell imaging has significantly modernized and became a ubiquitous tool in cell biology laboratories. Light microscopy was the earliest technique used in biology and has remained a main staple of cell biology and continues to evolve into more advanced techniques, which has been fueled by developments in computation, image detection devices, labelling, and sample preparation strategies. Some technological advances in this field include single molecule localization microscopy for imaging of living cells at or near nanometer spatial resolution. CRISPR-labeled fluorescence imaging of 3D structure of the genome in live cells. Brillouin microscopy to probe the viscoelastic properties of biological samples with diffraction-limited resolution in 3D. Holo-tomographic microscopy that quantitates phase microscopy into a hologram. High content-analysis microscopy that can track cells in 2D over time as they grow continuously over extended times by integrating microscopy inside incubators. Light sheet fluorescence microscopy that is designed to illuminate only the thin imaging focal planes, thus minimizing out-of-focus fluorescence and photobleaching. In this session speakers will discuss some of these state-of-the-art imaging methods to evaluate different aspects of cell biology including signal transduction, regulatory responses to stress, and disease states.

10:30Introduction (B. L. Upham, C. L. Goodman, M. H. Hashimi, and M. ul Hasan)
10:40A-10Discovery and Development of Wound Healing Agents from Natural Sources- Brassinosteroids and Beyond
Debora Esposito, North Carolina State University
11:15A-11Illuminating the Biochemical Activity Architecture of the Cell
Jin Zhang, University of California, San Diego
11:50A-12Image Cytometry–based Method Used to Measure Cellular and Subcellular Biology for High-content Screening
Nicholas Radio, Thermo Fisher Scientific
12:25 Discussion
10:30 am – 12:30 pm

MODEL SYSTEMS FOR DEVELOPING CRISPR/CAS TECHNOLOGY IN PLANTS

Pacific E
PLANT SYMPOSIUMConveners: Shubha Subbarao, Bayer U. S. – Crop Science, and Jeff Beringer, Inari Agriculture
Read Session Description

Genome editing has the potential to speed up the pace of plant breeding and trait development. As researchers seek to gain a greater understanding of CRISPR/Cas and related technologies the need to rapidly test the effectiveness of a multitude of editing concepts has become a pressing need. Rapid assay systems provide the opportunity to evaluate large numbers of constructs without engaging in the lengthy regeneration of mature plants, thereby helping manage costs and resources effectively.

10:30

 

Introduction (S. Subbarao and J. Beringer)

10:35

P_20

Application of Plant Rapid Expression Systems in Accelerating Gene Expression and Gene Editing Research

Peizhen Yang, Bayer Crop Science

11:00

P-21

Pre-selection of Guide RNAs Enables Efficient Genome Editing in Planta

Yan Liang, Joint Genome Institute

11:25

P-22

CoverCress – A Novel Oilseed Winter Crop with Canola-like Composition That Helps Sequester Carbon and Prevent Soil Erosion

Tim Ulmasov, CoverCress Inc.

11:50

P-23

Transforming Blackberry from Niche Crop to High Throughput Platform for Precision Breeding Technologies

Raj Deepika Chauhan, Pairwise

12:15

 

Discussion

12:30 pmAnnouncement of the Mission Bay Silent Auction WinnersPacific Foyer
12:30 pm – 1:30 pm2023 Program Planning and Development Committee MeetingPalm 7
1:30 pm – 2:30 pm

GENETIC ENGINEERING AND METABOLIC ENGINEERING

Pacific A
PLANT INTERACTIVE
POSTER SESSION
Moderator: Heqiang Huo, University of Florida

P-3012

Increased Engineering and Editing Efficiency of Sorghum bicolor Using Morphogene-assisted Transformation

Kiflom Aregawi, University of California, Berkeley, Jianqiang Shen, Grady Pierroz, Manoj K. Sharma, Jeffery Dahlberg, Judith Owiti, and Peggy G. Lemaux

P-3013

Inducible Expression for Acceleration of Design-Build-Test-Learn Cycles in the Metabolic Engineering of Oilcane

Moni Qiande, University of Florida – IFAS, Dang Viet Cao, Hui Liu, John Shanklin, and Fredy Altpeter

P-3014

Application of Developmental Regulators to Improve In-Planta or In Vitro Transformation in Plants

Heqiang Huo, University of Florida, Zhaoyuan Lian, Chi Dinh Nguyen, Jianjun Chen, Sandra Wilson, and Peggy Ozias-Akins

P-3015

Assessment of Maize Lethal Necrosis Disease Severity at Various Stages of Five cv. of Maize Crop

M. Qais, Georg-August-University, and A. Abbas

P-3016

Protein Engineering in Chlamydomonas reinhardtii for Improved Production of Recombinant Proteins

Corbin England, Arkansas State University, and Jianfeng Xu

P-3017

Development of an In Vitro Regeneration and Transformation System for Hop (Humulus lupulus)

Christopher J. Willig, Oregon State University, Michele S. Wiseman, John A. Henning, David H. Gent, and Steven H. Straus

1:30 pm – 2:30 pm

IN VITRO ANIMAL CELL SCIENCES

Pacific A
ANIMAL INTERACTIVE
POSTER SESSION
Moderator: Vivian Dayeh, University of Waterloo

A-2000

Signaling Pathways of Modulated Electro-hyperthermia Induced Tumor Cell Killing

Enikő Major, Semmelweis University, Andrea Balogh, Balázs Besztercei, Anett Benedek, and Zoltán Benyó

A-2001

Toxic Assessment of Environmental Contaminants Using a Novel In Vitro High Throughput Bioassay Screening System of Gap Junctional Intercellular Communication

Brad L. Upham, Michigan State University, Lizbeth Lockwood, Jamie Liebold, Joo Hye Yeo, Jinu Lee, Alison K. Bauer, Erika Lisabeth, and Richard Neubig

A-2002

Effects of Sodium Butyrate and D- and L-lactate on Rainbow Trout Intestinal Epithelial Cells

Daylan Pritchard, University of the Fraser Valley, Eryn Braley, Patrick G. Pumputis, Vivian R. Dayeh, Niels C. Bols, and Lucy E. J. Lee

A-2003

Explant Cultures of Rainbow Trout Olfactory Rosettes (ORs): Cell Outgrowth, Degeneration, Propagation, and Characterization

R. Goldbach, University of the Fraser Valley, J. B. Lee, N. C. Bols, and L. E. J. Lee

A-3000

The Establishment of a Sponge Cell Hybridoma

Cassady Dougan, Florida Atlantic University, Megan Conkling, Peter J. McCarthy, Amy E. Wright, and Shirley A. Pomponi

A-3001

ECEL1, PIEZO1 and NAV2 Genes Are Altered in the Enhanced and Directed Axonal Growth in Dorsal Root Ganglia Grown Ex Vivo on Nano-scale Grooved Topographical Surfaces

Julie Ellen Tamayo, Midwestern University, Kelly Keeler, Joshua Z. Gasiorowski, Michele Fornaro, and Kolla Kristjansdottir

1:30 pm – 2:30 pm

MICROPROPAGATION/MEDICINAL PLANTS

Pacific A
PLANT INTERACTIVE
POSTER SESSION
Moderator: Kristian Adamek, University of Guelph
P-3006Mutation Rates in Micropropagated Cannabis sativa Detected Through Genotyping by Sequencing, Are SSRs Antiquated?
Kristian Adamek, University of Guelph, Andrew Maxwell Phineas Jones, and Davoud Torkamaneh
P-3007Direct Field-to-Lab Introduction of Shoot Tips and Nodal Sections from Wild Nuttal’s Scrub Oak (Quercus dumosa) for the Purpose of Ex Situ Conservation Via Micropropagation
J. Ree, San Diego Zoo Wildlife Alliance, and C. Powell
P-3008Clonal Propagation of Avocado (Persea americana) from Adult Trees: Establishment In Vitro
 J. Tin, The Huntington Library, Art Museum, and Botanical Gardens, and R. Folgado
P-3009Isolation and Characterization of Two Novel Chryseobacterium sp. Genomotypes from the Rootzone of the Medicinal Plant Datura inoxia by Whole Genome Sequencing
Savanah Senn, Los Angeles Pierce College, Kelly Pangell, Maryam Saraylou, Adrianna Bowerman, Karu Smith, and Ryan O’halloran
P-3010Moringa oleifera Leaf Extract as a Potential Plant Growth Stimulant to Enhance Salt Stress Tolerance in Wheat
Talaat Ahmed, Qatar University, Mustafa Elshiekh, and Muhammad Fasih Khalid
2:30 pm – 3:30 pmPoster Session
Odd Poster Authors will present
See list of posters
Pacific A
3:30 pm – 4:00 pmPoster Breakdown and RemovalPacific A
3:30 pm – 5:00 pm

GENETIC TRANSFORMATION AND REGENERATION OF RECALCITRANT SPECIES (I.E., FRUIT TREES, ORPHAN CROPS), CHALLENGES AND WAY FORWARD

Pacific C&D
PLANT SYMPOSIUMConveners:     Carlos M. Hernandez-Garcia, CTC Genomics, Juan Debernardi, University of California, Davis, and Pamela Vogel, Pairwise
Read Session Description

Plant genetic transformation has been fundamental to enable basic research in plant biology and has revolutionized commercial agriculture. Unfortunately, robust, reproducible, and efficient plant transformation methods remain highly genotype-dependent and have been limited to a few species and selected genotypes of major crops. Genome editing provides novel opportunities for both basic research and product development; however, its rapid adoption within the scientific community has clearly evidenced major bottlenecks in the transformation and regeneration processes. Interestingly, major recent breakthroughs in the use of developmental genes such as GRF, BBM, WUS have led to dramatic improvements in transformation and regeneration efficiencies in monocots and have broaden the number of species and genotypes amenable to transformation. This revives the hope for the development of more universal and genotype independent transformation protocols. The use of these developmental genes is also expected to be expanded to more local crops where recalcitrance is often a significant challenge for genetic engineering, further enabling research groups with less specialized expertise to succeed in transformation. In this session we will review the status of the transformation of recalcitrant crops and discuss about potential future developments in the field.

3:30

 

Introduction (C. M. Hernandez-Garcia, J. Debernardi, and P. Vogel)

3:35

P-24

Of Media and Miracles: Successes and Frustrations in the Search for Efficient Regeneration and Transformation Methods for Trees and Crops

Steven Strauss, Oregon State University

4:00

P-25

A CRISPR-combo Approach for Speed Breeding and Regeneration of Genome-edited Plants

Yiping Qi, University of Maryland-College Park

4:25

P-26

Efficient Plant Regeneration from Protoplasts Isolated from Multiple Grape Genotypes and Demonstration of PEG-mediated Gene Editing Using CRISPR/Cas9

David Tricoli, University of California-Davis

4:50

 

Discussion

3:30 pm – 5:00 pm

IN VITRO TECHNOLOGIES FOR PLANT CONSERVATION AND GENE BANKING

Pacific E
PLANT SYMPOSIUMConvener:       Valerie C. Pence, Cincinnati Zoo & Botanical Garden, and Lori Marcum, Corteva Agriscience
Read Session Description

With the increasing threats of habitat loss, unsustainable use, and climate change to the world’s plant biodiversity, the need for ex situ conservation of plant genetic resources is also increasing. This is most efficiently accomplished with seed banks, but there are predicted to be tens of thousands of species for which conventional seed banking will be unworkable. These species are collectively known as exceptional plants and most will require cryopreservation methods in order to conserve them long-term. In vitro methods will be critical for many of these species, for providing tissues for cryopreservation and for propagating and recovering plants to restore species in the wild. Thus, there is an urgent need for developing in vitro protocols for a wide range of threatened plant taxa. This session provides a look at the work of three laboratories that are focused on this goal, representing a variety of species and illustrating the approaches and challenges of this work.

3:30

 

Introduction (V. C. Pence and L. Marcum)

3:35

P-27

In Vitro Methods for the Conservation of Rare Plants of the Southeastern U.S.

Emily Coffey, Atlanta Botanical Garden

4:00

P-28

The Creation of In Vitro Collections to Conserve Forest Genetic Resources: The Magnolia and Avocado Cases

Raquel Folgado, The Huntington Library, Art Museum, and Botanical Garden

4:25

P-29

Ex-Situ Conservation of Critically Endangered Species at the Hawaiian Rare Plant Program

Devon Gordon, The University of Hawai’i at Mãnoa

4:50

 

Discussion

3:30 pm – 5:00 pm

SINGLE CELL TECHNOLOGY AND APPLICATION IN BIOMEDICAL RESEARCH

Pacific H&I
ANIMAL SYMPOSIUM Conveners:       Kristina Martinez-Guryn, Midwestern University and Rosa Ventrella, Midwestern University 
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Single-cell omics technologies, such as genomics, epigenomics, transcriptomics, proteomics, and metabolomics have undergone rapid growth over the past decade. These technologies can analyze many cells at a high resolution making it a valuable tool in characterizing rare cell types and better understanding diverse cell states. Studying the cell-cell variation within cell populations can reveal unique mechanisms related to disease pathogenesis. The goal of this session is to describe the current state of research utilizing single-cell technologies and provide key examples of how these technologies are being used to identify how cellular heterogeneity contributes to human disease.

3:30

 

Introduction (R. Ventrella)

3:35

A-13

Use of Single-cell Approaches to Investigate the Role of T Cell Memory in Health and Disease
John Chang, University of California, San Diego

4:00

A-14

Defining Epithelial Development at the Single Cell Level
Scot Atwood, University of California, Irvine

4:25

A-15

Utilizing Single Cell Analyses to Characterize Models of Human Cortical Organoids
Aparna Bhaduri, University of California, Los Angeles

4:50

 

Discussion

5:00 pm – 5:30 pmSIVB Business Meeting
(All Members Are Urged to Attend)
Student Award Presentations
Pacific F&G
5:45 pm – 9:30 pmShips of the Seven Seas, Tuesday Evening at the Maritime Museum
Admittance by Advanced Ticket Holders only
Off Property