The Society for In Vitro Biology is pleased to share our new webinar series, “A Closer Look: Learning from the Past, Considering the Future.” This webinar series features experts from a variety of different subject matters who provide historical reviews on topics of interest to the SIVB community. Join your colleagues for these special programs held throughout the year and check back to see new installments as they are announced.
Registration is free for all current members of SIVB.
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|January 26, 2021
2:00 pm ESTProf. Arun K. Bhunia, BvSc, PhDProfessor of Food Microbiology in the Department of Food Science at Purdue University, affiliated with the Department of Comparative Pathobiology, Purdue Institute of Inflammation, Immunology and Infectious Disease (PI4D), and Purdue University Life Science (PULSe), and Chair of the Food Science Graduate Program
Receptor-targeted next-generation bioengineered probiotics to improve gut health and prevent infectious disease
The gastrointestinal mucosa represents the first site for the dynamic interaction of the enteric pathogens with the host. Therefore, averting this critical pathogen interaction step, especially binding to host cell receptors, should help prevent infection. Two major host cell receptors, E-cadherin and Hsp60 used by Listeria monocytogenes Internalin A (InlA) and Listeria adhesion protein (LAP), respectively, were used as targets to generate our next-generation bioengineered Lactobacillus probiotics. Though the InlA-expressing probiotic was effective in vitro, LAP-expressing probiotic significantly dampened NF-?B signaling and myosin light-chain kinase (MLCK) activity and protected intestinal epithelial barrier integrity, reduced inflammatory response, and modulated immune response in mice. This probiotic also prevented fetoplacental transmission of Listeria in pregnant guinea pigs. The rational design of bioengineered probiotics targeting host cell receptors could be useful for not only improving gut health due to intimate contact of probiotics with enterocytes but also preventing infectious diseases
Professor Bhunia’s expertise is in the area of microbial pathogenesis, probiotic bioengineering, and foodborne pathogen detection. To date, he has co-authored 204 peer-reviewed research publications, 2 textbooks (Fundamental Food Microbiology and Foodborne Microbial Pathogens ), edited 4 books, and delivered over 155 talks. He served in the USDA National Advisory Committee on Microbiological Criteria for Foods (NACMCF) and has received Purdue Agriculture Research Award, Purdue Faculty Scholar, Purdue Outstanding Graduate Educator, IFT R&D Award Fulbright Specialist, and Maurice Weber Laboratorian Award (IAFP). He is serving as the Editor-in-Chief of Foods, and an Associate Editor for Frontiers in Microbiology, PLoS One, Frontiers in Sustainable Food Systems, and BMC Microbiology.
|October 26, 2021
Dr. Adam Sowalsky, PhD
Investigator in the Laboratory of Genitourinary Cancer Pathogenesis at the National Cancer Institute
|Tumor evolution as a window into prostate cancer treatment resistance
Like most human solid tumors, localized prostate is readily curable if treated early but metastatic disease is invariably lethal. The Androgen Receptor (AR) transcription factor is well established as the molecular driver of prostate cancer, and efforts to target AR show tremendous therapeutic efficacy. Nonetheless, resistance to AR-targeted therapies always develop with time. For the last decade, our laboratory examined the relationship between prostate tumor aggressivity and intratumoral heterogeneity, using patient specimens from phase two clinical trials as human model systems of tumor evolution. Although evolutionary processes related to diversity are known to be associated with increased fitness, our laboratory has recently established a direct correlation between treatment response to AR-targeted therapies and the extent of within-patient diversity prior to therapy. In this presentation, I will describe the historical context of treatment for prostate cancer, recent advances in understanding genomic alterations driving prostate cancer progression, and how the timing of these events sets the fate of a tumor to progress or respond to therapy. I will also describe efforts to improve in vitro models of prostate cancer that recapitulate the genomic complexity of disease and recent progress to target mechanisms of resistance to AR-targeted therapies.
|April 28, 2021
Dr. Stanton Gelvin, PhD
H. Edwin Umbarger Distinguished Professor of Biological Sciences at Purdue University
|Plant genes important for T-DNA Trafficking and integration in plant cells
Agrobacterium-mediated plant genetic transformation is a core technology for basic plant science and for the agricultural biotechnology industry. More than 40 years of intensive research has given us a good, if not complete, understanding of early transformation events that occur within the bacterium. This understanding has guided the research community in generating novel Agrobacterium strains with increased virulence. However, many plant species, or particular varieties/cultivars, remain highly recalcitrant to Agrobacterium-mediated transformation. To some extent, this recalcitrance is a tissue culture problem, but our understanding of the molecular events that occur in the plant cell, and plant genes/proteins contributing to transformation, lag far behind our knowledge of the events that occur within the bacterium. For the past ~25 years, our laboratory has investigated transformation processes that occur within the plant cell. Our emphasis has been on identifying plant proteins, often interacting with transferred Agrobacterium virulence (vir) effector proteins, that mediate T-strand cytoplasmic trafficking, nuclear entry, and integration into the plant genome. In this webinar, I shall discuss our current knowledge of processes which occur within the plant cell that underlie successful transformation. I shall emphasize our current understanding of how T-DNA integrates into plant chromosomes.